Restriction map for
pF1KB6722
(Circular) (Six-base) MAPSORT of: pF1KB6722.seq Check: 6091 from: 1 to: 3515
pF1KB6722 3515 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 553 553
Size: 3515
Acc65I G'GTAC_C
Cuts at: 531 531
Size: 3515
AclI AA'CG_TT
Cuts at: 3355 3355
Size: 3515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1340 1772 2519 2787 1340
Size: 432 747 268 2068
Fragments arranged by size:
2068 747 432 268
AfeI AGC'GCT
Cuts at: 313 313
Size: 3515
AflIII A'CryG_T
Cuts at: 867 1971 2948 867
Size: 1104 977 1434
Fragments arranged by size:
1434 1104 977
AgeI A'CCGG_T
Cuts at: 1903 1903
Size: 3515
AlwNI CAG_nnn'CTG
Cuts at: 120 363 375 820 2387 120
Size: 243 12 445 1567 1248
Fragments arranged by size:
1567 1248 445 243 12
ApaLI G'TGCA_C
Cuts at: 2285 2285
Size: 3515
ApoI r'AATT_y
Cuts at: 519 2868 3124 519
Size: 2349 256 910
Fragments arranged by size:
2349 910 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3515
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3515
AvaI C'yCGr_G
Cuts at: 535 535
Size: 3515
BamHI G'GATC_C
Cuts at: 540 540
Size: 3515
BanI G'GyrC_C
Cuts at: 531 945 1193 1228 531
Size: 414 248 35 2818
Fragments arranged by size:
2818 414 248 35
BanII G_rGCy'C
Cuts at: 192 350 463 529 1559 192
Size: 158 113 66 1030 2148
Fragments arranged by size:
2148 1030 158 113 66
BbeI G_GCGC'C
Cuts at: 949 1197 949
Size: 248 3267
BciVI GTATCCnnnnn_n'
Cuts at: 1413 2174 3515 1413
Size: 761 1341 1413
Fragments arranged by size:
1413 1341 761
BclI T'GATC_A
Cuts at: 1035 1035
Size: 3515
BglII A'GATC_T
Cuts at: 1030 3067 1030
Size: 2037 1478
BlpI GC'TnA_GC
Cuts at: 627 627
Size: 3515
Bme1580I G_kGCm'C
Cuts at: 1140 1233 2289 1140
Size: 93 1056 2366
Fragments arranged by size:
2366 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 727 808 1143 3287 727
Size: 81 335 2144 955
Fragments arranged by size:
2144 955 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1882 1882
Size: 3515
Bpu10I CC'TnA_GC
Cuts at: 191 270 191
Size: 79 3436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 691 1570 2062 2360 190
Size: 501 879 492 298 1345
Fragments arranged by size:
1345 879 501 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3219 3219
Size: 3515
BsaAI yAC'GTr
Cuts at: 868 868
Size: 3515
BsaBI GATnn'nnATC
Cuts at: 6 1029 3066 6
Size: 1023 2037 455
Fragments arranged by size:
2037 1023 455
BsaHI Gr'CG_yC
Cuts at: 946 1194 1896 3392 946
Size: 248 702 1496 1069
Fragments arranged by size:
1496 1069 702 248
BsaWI w'CCGG_w
Cuts at: 251 704 1225 1903 2177 2324 2735 251
Size: 453 521 678 274 147 411 1031
Fragments arranged by size:
1031 678 521 453 411 274 147
BseYI C'CCAG_C
Cuts at: 285 940 1698 2275 2771 285
Size: 655 758 577 496 1029
Fragments arranged by size:
1029 758 655 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 376 353
Size: 23 3492
BsiEI CG_ry'CG
Cuts at: 104 1057 1103 2311 104
Size: 953 46 1208 1308
Fragments arranged by size:
1308 1208 953 46
BsiHKAI G_wGCw'C
Cuts at: 350 529 1307 1497 2289 350
Size: 179 778 190 792 1576
Fragments arranged by size:
1576 792 778 190 179
Bsp1286I G_dGCh'C
Cuts at: 192 350 463 529 1140 1233 1307 1497 1559
Size: 158 113 66 611 93 74 190 62
Cuts at: 1559 2289 192
Size: 730 1418
Fragments arranged by size:
1418 730 611 190 158 113 93 74
66 62
BspEI T'CCGG_A
Cuts at: 2735 2735
Size: 3515
BspHI T'CATG_A
Cuts at: 2691 2691
Size: 3515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 565 779 1081 1462 565
Size: 214 302 381 2618
Fragments arranged by size:
2618 381 302 214
BsrBI CCG'CTC
Cuts at: 50 486 1807 1861 3345 3510 50
Size: 436 1321 54 1484 165 55
Fragments arranged by size:
1484 1321 436 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1427 1427
Size: 3515
BsrFI r'CCGG_y
Cuts at: 1513 1694 1903 1513
Size: 181 209 3125
Fragments arranged by size:
3125 209 181
BssSI C'ACGA_G
Cuts at: 1786 2144 1786
Size: 358 3157
BstAPI GCAn_nnn'nTGC
Cuts at: 366 892 366
Size: 526 2989
BstBI TT'CG_AA
Cuts at: 1876 1876
Size: 3515
BstYI r'GATC_y
Cuts at: 1 540 1007 1030 1365 1611 2623 2709 2721
Size: 539 467 23 335 246 1012 86 12
Cuts at: 2721 3067 1
Size: 346 449
Fragments arranged by size:
1012 539 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3515
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1465 1646 1809 1465
Size: 181 163 3171
Fragments arranged by size:
3171 181 163
BtsI GCAGTG_nn'
Cuts at: 183 758 183
Size: 575 2940
DraI TTT'AAA
Cuts at: 514 514
Size: 3515
DrdI GACnn_nn'nnGTC
Cuts at: 486 1221 2079 486
Size: 735 858 1922
Fragments arranged by size:
1922 858 735
EaeI y'GGCC_r
Cuts at: 1100 1274 1665 1692 3037 1100
Size: 174 391 27 1345 1578
Fragments arranged by size:
1578 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1100 1100
Size: 3515
EarI CTCTTCn'nnn_
Cuts at: 1538 1748 1538
Size: 210 3305
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1723 2033 2179 3325 1723
Size: 310 146 1146 1913
Fragments arranged by size:
1913 1146 310 146
EcoICRI GAG'CTC
Cuts at: 348 527 348
Size: 179 3336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1340 1772 2519 2787 1340
Size: 432 747 268 2068
Fragments arranged by size:
2068 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 167 431 654 167
Size: 264 223 3028
Fragments arranged by size:
3028 264 223
EcoRI G'AATT_C
Cuts at: 519 519
Size: 3515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1534 1534
Size: 3515
FspI TGC'GCA
Cuts at: 1296 1296
Size: 3515
HaeII r_GCGC'y
Cuts at: 315 949 1197 2219 3198 315
Size: 634 248 1022 979 632
Fragments arranged by size:
1022 979 634 632 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 357 389 1357 1387 1389 1419 357
Size: 32 968 30 2 30 2453
Fragments arranged by size:
2453 968 32 30 30 2
HincII GTy'rAC
Cuts at: 554 554
Size: 3515
HindIII A'AGCT_T
Cuts at: 267 267
Size: 3515
KasI G'GCGC_C
Cuts at: 945 1193 945
Size: 248 3267
KpnI G_GTAC'C
Cuts at: 535 535
Size: 3515
MluI A'CGCG_T
Cuts at: 2948 2948
Size: 3515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2186 2370 2186
Size: 184 3331
MscI TGG'CCA
Cuts at: 1276 1276
Size: 3515
MslI CAynn'nnrTG
Cuts at: 1504 1631 1504
Size: 127 3388
MspA1I CmG'CkG
Cuts at: 363 626 940 1300 2313 2558 363
Size: 263 314 360 1013 245 1320
Fragments arranged by size:
1320 1013 360 314 263 245
NaeI GCC'GGC
Cuts at: 1696 1696
Size: 3515
NarI GG'CG_CC
Cuts at: 946 1194 946
Size: 248 3267
NcoI C'CATG_G
Cuts at: 107 107
Size: 3515
NgoMIV G'CCGG_C
Cuts at: 1694 1694
Size: 3515
NspI r_CATG'y
Cuts at: 568 1068 1975 568
Size: 500 907 2108
Fragments arranged by size:
2108 907 500
PasI CC'CwG_GG
Cuts at: 408 408
Size: 3515
PciI A'CATG_T
Cuts at: 1971 1971
Size: 3515
PmeI GTTT'AAAC
Cuts at: 514 514
Size: 3515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2654 2686 2654
Size: 32 3483
PpuMI rG'GwC_Cy
Cuts at: 167 167
Size: 3515
PstI C_TGCA'G
Cuts at: 418 562 1247 418
Size: 144 685 2686
Fragments arranged by size:
2686 685 144
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3515
PvuII CAG'CTG
Cuts at: 363 940 1300 363
Size: 577 360 2578
Fragments arranged by size:
2578 577 360
RsrII CG'GwC_CG
Cuts at: 1710 1710
Size: 3515
SacI G_AGCT'C
Cuts at: 350 529 350
Size: 179 3336
SalI G'TCGA_C
Cuts at: 552 552
Size: 3515
SapI GCTCTTCn'nnn_
Cuts at: 1538 1748 1538
Size: 210 3305
SbfI CC_TGCA'GG
Cuts at: 562 562
Size: 3515
ScaI AGT'ACT
Cuts at: 3032 3032
Size: 3515
SfcI C'TryA_G
Cuts at: 33 414 558 1243 2236 2427 33
Size: 381 144 685 993 191 1121
Fragments arranged by size:
1121 993 685 381 191 144
SfoI GGC'GCC
Cuts at: 947 1195 947
Size: 248 3267
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3515
SmaI CCC'GGG
Cuts at: 537 537
Size: 3515
SmlI C'TyrA_G
Cuts at: 205 670 1585 2077 2339 205
Size: 465 915 492 262 1381
Fragments arranged by size:
1381 915 492 465 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3515
SphI G_CATG'C
Cuts at: 568 1068 568
Size: 500 3015
StyI C'CwwG_G
Cuts at: 107 171 233 649 1003 107
Size: 64 62 416 354 2619
Fragments arranged by size:
2619 416 354 64 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 342 1098 1231 1690 1899 342
Size: 756 133 459 209 1958
Fragments arranged by size:
1958 756 459 209 133
TatI w'GTAC_w
Cuts at: 209 3030 209
Size: 2821 694
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3032 3064 3032
Size: 32 3483
Tth111I GACn'n_nGTC
Cuts at: 1312 1312
Size: 3515
XbaI T'CTAG_A
Cuts at: 546 546
Size: 3515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 178 347 1010 178
Size: 169 663 2683
Fragments arranged by size:
2683 663 169
XmaI C'CCGG_G
Cuts at: 535 535
Size: 3515
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI