Restriction map for
pF1KB6718
(Circular) (Six-base) MAPSORT of: pF1KB6718.seq Check: 198 from: 1 to: 3515
pF1KB6718 3515 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 553 553
Size: 3515
Acc65I G'GTAC_C
Cuts at: 531 531
Size: 3515
AclI AA'CG_TT
Cuts at: 3355 3355
Size: 3515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 1340 1772 2519 2787 320
Size: 1020 432 747 268 1048
Fragments arranged by size:
1048 1020 747 432 268
AflIII A'CryG_T
Cuts at: 155 867 1971 2948 155
Size: 712 1104 977 722
Fragments arranged by size:
1104 977 722 712
AgeI A'CCGG_T
Cuts at: 1903 1903
Size: 3515
AlwNI CAG_nnn'CTG
Cuts at: 820 2387 820
Size: 1567 1948
ApaLI G'TGCA_C
Cuts at: 294 2285 294
Size: 1991 1524
ApoI r'AATT_y
Cuts at: 176 519 2868 3124 176
Size: 343 2349 256 567
Fragments arranged by size:
2349 567 343 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3515
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3515
AvaI C'yCGr_G
Cuts at: 535 535
Size: 3515
BamHI G'GATC_C
Cuts at: 540 540
Size: 3515
BanI G'GyrC_C
Cuts at: 531 945 1193 1228 531
Size: 414 248 35 2818
Fragments arranged by size:
2818 414 248 35
BanII G_rGCy'C
Cuts at: 529 1559 529
Size: 1030 2485
BbeI G_GCGC'C
Cuts at: 949 1197 949
Size: 248 3267
BciVI GTATCCnnnnn_n'
Cuts at: 1413 2174 3515 1413
Size: 761 1341 1413
Fragments arranged by size:
1413 1341 761
BclI T'GATC_A
Cuts at: 1035 1035
Size: 3515
BglII A'GATC_T
Cuts at: 1030 3067 1030
Size: 2037 1478
BlpI GC'TnA_GC
Cuts at: 627 627
Size: 3515
Bme1580I G_kGCm'C
Cuts at: 298 1140 1233 2289 298
Size: 842 93 1056 1524
Fragments arranged by size:
1524 1056 842 93
BmrI ACTGGGnnnn_n'
Cuts at: 727 808 1143 3287 727
Size: 81 335 2144 955
Fragments arranged by size:
2144 955 335 81
BmtI G_CTAG'C
Cuts at: 139 139
Size: 3515
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1882 1882
Size: 3515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 349
Size: 3515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 1570 2062 2360 691
Size: 879 492 298 1846
Fragments arranged by size:
1846 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 196 3219 196
Size: 3023 492
BsaAI yAC'GTr
Cuts at: 156 868 156
Size: 712 2803
BsaBI GATnn'nnATC
Cuts at: 6 1029 3066 6
Size: 1023 2037 455
Fragments arranged by size:
2037 1023 455
BsaHI Gr'CG_yC
Cuts at: 946 1194 1896 3392 946
Size: 248 702 1496 1069
Fragments arranged by size:
1496 1069 702 248
BsaWI w'CCGG_w
Cuts at: 704 1225 1903 2177 2324 2735 704
Size: 521 678 274 147 411 1484
Fragments arranged by size:
1484 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 940 1698 2275 2771 940
Size: 758 577 496 1684
Fragments arranged by size:
1684 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1057 1103 2311 104
Size: 953 46 1208 1308
Fragments arranged by size:
1308 1208 953 46
BsiHKAI G_wGCw'C
Cuts at: 298 529 1307 1497 2289 298
Size: 231 778 190 792 1524
Fragments arranged by size:
1524 792 778 231 190
Bsp1286I G_dGCh'C
Cuts at: 298 529 1140 1233 1307 1497 1559 2289 298
Size: 231 611 93 74 190 62 730 1524
Fragments arranged by size:
1524 730 611 231 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2735 2735
Size: 3515
BspHI T'CATG_A
Cuts at: 2691 2691
Size: 3515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 470 565 779 1081 1462 470
Size: 95 214 302 381 2523
Fragments arranged by size:
2523 381 302 214 95
BsrBI CCG'CTC
Cuts at: 50 1807 1861 3345 3510 50
Size: 1757 54 1484 165 55
Fragments arranged by size:
1757 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1427 1427
Size: 3515
BsrFI r'CCGG_y
Cuts at: 1513 1694 1903 1513
Size: 181 209 3125
Fragments arranged by size:
3125 209 181
BsrGI T'GTAC_A
Cuts at: 273 273
Size: 3515
BssSI C'ACGA_G
Cuts at: 1786 2144 1786
Size: 358 3157
BstAPI GCAn_nnn'nTGC
Cuts at: 892 892
Size: 3515
BstBI TT'CG_AA
Cuts at: 1876 1876
Size: 3515
BstYI r'GATC_y
Cuts at: 1 540 1007 1030 1365 1611 2623 2709 2721
Size: 539 467 23 335 246 1012 86 12
Cuts at: 2721 3067 1
Size: 346 449
Fragments arranged by size:
1012 539 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1465 1646 1809 1465
Size: 181 163 3171
Fragments arranged by size:
3171 181 163
BtsI GCAGTG_nn'
Cuts at: 211 758 211
Size: 547 2968
DraI TTT'AAA
Cuts at: 514 514
Size: 3515
DraIII CAC_nnn'GTG
Cuts at: 494 494
Size: 3515
DrdI GACnn_nn'nnGTC
Cuts at: 1221 2079 1221
Size: 858 2657
EaeI y'GGCC_r
Cuts at: 1100 1274 1665 1692 3037 1100
Size: 174 391 27 1345 1578
Fragments arranged by size:
1578 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1100 1100
Size: 3515
EarI CTCTTCn'nnn_
Cuts at: 1538 1748 1538
Size: 210 3305
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1723 2033 2179 3325 1723
Size: 310 146 1146 1913
Fragments arranged by size:
1913 1146 310 146
EcoICRI GAG'CTC
Cuts at: 527 527
Size: 3515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 349 1340 1772 2519 2787 320
Size: 29 991 432 747 268 1048
Fragments arranged by size:
1048 991 747 432 268 29
EcoO109I rG'GnC_Cy
Cuts at: 654 654
Size: 3515
EcoRI G'AATT_C
Cuts at: 519 519
Size: 3515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 490 1534 490
Size: 1044 2471
FspI TGC'GCA
Cuts at: 1296 1296
Size: 3515
HaeII r_GCGC'y
Cuts at: 949 1197 2219 3198 949
Size: 248 1022 979 1266
Fragments arranged by size:
1266 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1357 1387 1389 1419 1357
Size: 30 2 30 3453
Fragments arranged by size:
3453 30 30 2
HincII GTy'rAC
Cuts at: 554 554
Size: 3515
KasI G'GCGC_C
Cuts at: 945 1193 945
Size: 248 3267
KpnI G_GTAC'C
Cuts at: 535 535
Size: 3515
MluI A'CGCG_T
Cuts at: 2948 2948
Size: 3515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2186 2370 2186
Size: 184 3331
MscI TGG'CCA
Cuts at: 1276 1276
Size: 3515
MslI CAynn'nnrTG
Cuts at: 112 1504 1631 112
Size: 1392 127 1996
Fragments arranged by size:
1996 1392 127
MspA1I CmG'CkG
Cuts at: 626 940 1300 2313 2558 626
Size: 314 360 1013 245 1583
Fragments arranged by size:
1583 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1696 1696
Size: 3515
NarI GG'CG_CC
Cuts at: 946 1194 946
Size: 248 3267
NgoMIV G'CCGG_C
Cuts at: 1694 1694
Size: 3515
NheI G'CTAG_C
Cuts at: 135 135
Size: 3515
NspI r_CATG'y
Cuts at: 568 1068 1975 568
Size: 500 907 2108
Fragments arranged by size:
2108 907 500
PciI A'CATG_T
Cuts at: 1971 1971
Size: 3515
PmeI GTTT'AAAC
Cuts at: 514 514
Size: 3515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2654 2686 2654
Size: 32 3483
PstI C_TGCA'G
Cuts at: 562 1247 562
Size: 685 2830
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3515
PvuII CAG'CTG
Cuts at: 940 1300 940
Size: 360 3155
RsrII CG'GwC_CG
Cuts at: 1710 1710
Size: 3515
SacI G_AGCT'C
Cuts at: 529 529
Size: 3515
SalI G'TCGA_C
Cuts at: 552 552
Size: 3515
SapI GCTCTTCn'nnn_
Cuts at: 1538 1748 1538
Size: 210 3305
SbfI CC_TGCA'GG
Cuts at: 562 562
Size: 3515
ScaI AGT'ACT
Cuts at: 3032 3032
Size: 3515
SfcI C'TryA_G
Cuts at: 33 558 1243 2236 2427 33
Size: 525 685 993 191 1121
Fragments arranged by size:
1121 993 685 525 191
SfoI GGC'GCC
Cuts at: 947 1195 947
Size: 248 3267
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3515
SmaI CCC'GGG
Cuts at: 537 537
Size: 3515
SmlI C'TyrA_G
Cuts at: 670 1585 2077 2339 670
Size: 915 492 262 1846
Fragments arranged by size:
1846 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3515
SphI G_CATG'C
Cuts at: 568 1068 568
Size: 500 3015
StyI C'CwwG_G
Cuts at: 206 649 1003 206
Size: 443 354 2718
Fragments arranged by size:
2718 443 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1098 1231 1690 1899 1098
Size: 133 459 209 2714
Fragments arranged by size:
2714 459 209 133
TatI w'GTAC_w
Cuts at: 273 3030 273
Size: 2757 758
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 162 194 3032 3064 162
Size: 32 2838 32 613
Fragments arranged by size:
2838 613 32 32
Tth111I GACn'n_nGTC
Cuts at: 1312 1312
Size: 3515
XbaI T'CTAG_A
Cuts at: 546 546
Size: 3515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 189 1010 189
Size: 821 2694
XmaI C'CCGG_G
Cuts at: 535 535
Size: 3515
XmnI GAAnn'nnTTC
Cuts at: 308 308
Size: 3515
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI