Restriction map for
pF1KB6715
(Circular) (Six-base) MAPSORT of: pF1KB6715.seq Check: 885 from: 1 to: 3356
pF1KB6715 3356 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 394 394
Size: 3356
Acc65I G'GTAC_C
Cuts at: 372 372
Size: 3356
AclI AA'CG_TT
Cuts at: 3196 3196
Size: 3356
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1181 1613 2360 2628 1181
Size: 432 747 268 1909
Fragments arranged by size:
1909 747 432 268
AflIII A'CryG_T
Cuts at: 708 1812 2789 708
Size: 1104 977 1275
Fragments arranged by size:
1275 1104 977
AgeI A'CCGG_T
Cuts at: 1744 1744
Size: 3356
AlwNI CAG_nnn'CTG
Cuts at: 277 661 2228 277
Size: 384 1567 1405
Fragments arranged by size:
1567 1405 384
ApaLI G'TGCA_C
Cuts at: 2126 2126
Size: 3356
ApoI r'AATT_y
Cuts at: 360 2709 2965 360
Size: 2349 256 751
Fragments arranged by size:
2349 751 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3356
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3356
AvaI C'yCGr_G
Cuts at: 376 376
Size: 3356
BamHI G'GATC_C
Cuts at: 381 381
Size: 3356
BanI G'GyrC_C
Cuts at: 372 786 1034 1069 372
Size: 414 248 35 2659
Fragments arranged by size:
2659 414 248 35
BanII G_rGCy'C
Cuts at: 370 1400 370
Size: 1030 2326
BbeI G_GCGC'C
Cuts at: 790 1038 790
Size: 248 3108
BbsI GAAGACnn'nnnn_
Cuts at: 130 274 130
Size: 144 3212
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3322
BciVI GTATCCnnnnn_n'
Cuts at: 1254 2015 3356 1254
Size: 761 1341 1254
Fragments arranged by size:
1341 1254 761
BclI T'GATC_A
Cuts at: 876 876
Size: 3356
BglII A'GATC_T
Cuts at: 871 2908 871
Size: 2037 1319
BlpI GC'TnA_GC
Cuts at: 468 468
Size: 3356
Bme1580I G_kGCm'C
Cuts at: 981 1074 2130 981
Size: 93 1056 2207
Fragments arranged by size:
2207 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 568 649 984 3128 568
Size: 81 335 2144 796
Fragments arranged by size:
2144 796 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1723 1723
Size: 3356
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 1411 1903 2201 532
Size: 879 492 298 1687
Fragments arranged by size:
1687 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3060 3060
Size: 3356
BsaAI yAC'GTr
Cuts at: 709 709
Size: 3356
BsaBI GATnn'nnATC
Cuts at: 6 870 2907 6
Size: 864 2037 455
Fragments arranged by size:
2037 864 455
BsaHI Gr'CG_yC
Cuts at: 787 1035 1737 3233 787
Size: 248 702 1496 910
Fragments arranged by size:
1496 910 702 248
BsaWI w'CCGG_w
Cuts at: 133 545 1066 1744 2018 2165 2576 133
Size: 412 521 678 274 147 411 913
Fragments arranged by size:
913 678 521 412 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 344 347 214
Size: 130 3 3223
Fragments arranged by size:
3223 130 3
BseYI C'CCAG_C
Cuts at: 781 1539 2116 2612 781
Size: 758 577 496 1525
Fragments arranged by size:
1525 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 898 944 2152 104
Size: 794 46 1208 1308
Fragments arranged by size:
1308 1208 794 46
BsiHKAI G_wGCw'C
Cuts at: 370 1148 1338 2130 370
Size: 778 190 792 1596
Fragments arranged by size:
1596 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 370 981 1074 1148 1338 1400 2130 370
Size: 611 93 74 190 62 730 1596
Fragments arranged by size:
1596 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2576 2576
Size: 3356
BspHI T'CATG_A
Cuts at: 2532 2532
Size: 3356
BspMI ACCTGCnnnn'nnnn_
Cuts at: 339 406 620 922 1303 339
Size: 67 214 302 381 2392
Fragments arranged by size:
2392 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 1648 1702 3186 3351 50
Size: 1598 54 1484 165 55
Fragments arranged by size:
1598 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1268 1268
Size: 3356
BsrFI r'CCGG_y
Cuts at: 1354 1535 1744 1354
Size: 181 209 2966
Fragments arranged by size:
2966 209 181
BssSI C'ACGA_G
Cuts at: 1627 1985 1627
Size: 358 2998
BstAPI GCAn_nnn'nTGC
Cuts at: 733 733
Size: 3356
BstBI TT'CG_AA
Cuts at: 1717 1717
Size: 3356
BstYI r'GATC_y
Cuts at: 1 381 848 871 1206 1452 2464 2550 2562
Size: 380 467 23 335 246 1012 86 12
Cuts at: 2562 2908 1
Size: 346 449
Fragments arranged by size:
1012 467 449 380 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1306 1487 1650 1306
Size: 181 163 3012
Fragments arranged by size:
3012 181 163
BtsI GCAGTG_nn'
Cuts at: 599 599
Size: 3356
DraI TTT'AAA
Cuts at: 355 355
Size: 3356
DraIII CAC_nnn'GTG
Cuts at: 315 315
Size: 3356
DrdI GACnn_nn'nnGTC
Cuts at: 1062 1920 1062
Size: 858 2498
EaeI y'GGCC_r
Cuts at: 941 1115 1506 1533 2878 941
Size: 174 391 27 1345 1419
Fragments arranged by size:
1419 1345 391 174 27
EagI C'GGCC_G
Cuts at: 941 941
Size: 3356
EarI CTCTTCn'nnn_
Cuts at: 1379 1589 1379
Size: 210 3146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1564 1874 2020 3166 1564
Size: 310 146 1146 1754
Fragments arranged by size:
1754 1146 310 146
EcoICRI GAG'CTC
Cuts at: 368 368
Size: 3356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1181 1613 2360 2628 1181
Size: 432 747 268 1909
Fragments arranged by size:
1909 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 495 495
Size: 3356
EcoRI G'AATT_C
Cuts at: 360 360
Size: 3356
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1375 1375
Size: 3356
FspI TGC'GCA
Cuts at: 1137 1137
Size: 3356
HaeII r_GCGC'y
Cuts at: 790 1038 2060 3039 790
Size: 248 1022 979 1107
Fragments arranged by size:
1107 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1198 1228 1230 1260 1198
Size: 30 2 30 3294
Fragments arranged by size:
3294 30 30 2
HincII GTy'rAC
Cuts at: 395 395
Size: 3356
KasI G'GCGC_C
Cuts at: 786 1034 786
Size: 248 3108
KpnI G_GTAC'C
Cuts at: 376 376
Size: 3356
MluI A'CGCG_T
Cuts at: 2789 2789
Size: 3356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2027 2211 2027
Size: 184 3172
MscI TGG'CCA
Cuts at: 1117 1117
Size: 3356
MslI CAynn'nnrTG
Cuts at: 1345 1472 1345
Size: 127 3229
MspA1I CmG'CkG
Cuts at: 277 467 781 1141 2154 2399 277
Size: 190 314 360 1013 245 1234
Fragments arranged by size:
1234 1013 360 314 245 190
NaeI GCC'GGC
Cuts at: 1537 1537
Size: 3356
NarI GG'CG_CC
Cuts at: 787 1035 787
Size: 248 3108
NgoMIV G'CCGG_C
Cuts at: 1535 1535
Size: 3356
NspI r_CATG'y
Cuts at: 409 909 1816 409
Size: 500 907 1949
Fragments arranged by size:
1949 907 500
PciI A'CATG_T
Cuts at: 1812 1812
Size: 3356
PmeI GTTT'AAAC
Cuts at: 355 355
Size: 3356
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2495 2527 2495
Size: 32 3324
PstI C_TGCA'G
Cuts at: 403 1088 403
Size: 685 2671
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3356
PvuII CAG'CTG
Cuts at: 277 781 1141 277
Size: 504 360 2492
Fragments arranged by size:
2492 504 360
RsrII CG'GwC_CG
Cuts at: 1551 1551
Size: 3356
SacI G_AGCT'C
Cuts at: 370 370
Size: 3356
SalI G'TCGA_C
Cuts at: 393 393
Size: 3356
SapI GCTCTTCn'nnn_
Cuts at: 1379 1589 1379
Size: 210 3146
SbfI CC_TGCA'GG
Cuts at: 403 403
Size: 3356
ScaI AGT'ACT
Cuts at: 2873 2873
Size: 3356
SexAI A'CCwGG_T
Cuts at: 311 311
Size: 3356
SfcI C'TryA_G
Cuts at: 33 164 240 399 1084 2077 2268 33
Size: 131 76 159 685 993 191 1121
Fragments arranged by size:
1121 993 685 191 159 131 76
SfoI GGC'GCC
Cuts at: 788 1036 788
Size: 248 3108
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3356
SmaI CCC'GGG
Cuts at: 378 378
Size: 3356
SmlI C'TyrA_G
Cuts at: 511 1426 1918 2180 511
Size: 915 492 262 1687
Fragments arranged by size:
1687 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3356
SphI G_CATG'C
Cuts at: 409 909 409
Size: 500 2856
StyI C'CwwG_G
Cuts at: 490 844 490
Size: 354 3002
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 939 1072 1531 1740 939
Size: 133 459 209 2555
Fragments arranged by size:
2555 459 209 133
TatI w'GTAC_w
Cuts at: 2871 2871
Size: 3356
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2873 2905 2873
Size: 32 3324
Tth111I GACn'n_nGTC
Cuts at: 1153 1153
Size: 3356
XbaI T'CTAG_A
Cuts at: 387 387
Size: 3356
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 851 851
Size: 3356
XmaI C'CCGG_G
Cuts at: 376 376
Size: 3356
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI