Restriction map for
pF1KB6702
(Circular) (Six-base) MAPSORT of: pF1KB6702.seq Check: 1739 from: 1 to: 3497
pF1KB6702 3497 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 159 463 159
Size: 304 3193
AccI GT'mk_AC
Cuts at: 535 535
Size: 3497
Acc65I G'GTAC_C
Cuts at: 513 513
Size: 3497
AclI AA'CG_TT
Cuts at: 3337 3337
Size: 3497
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 1322 1754 2501 2769 421
Size: 901 432 747 268 1149
Fragments arranged by size:
1149 901 747 432 268
AflIII A'CryG_T
Cuts at: 849 1953 2930 849
Size: 1104 977 1416
Fragments arranged by size:
1416 1104 977
AgeI A'CCGG_T
Cuts at: 1885 1885
Size: 3497
AlwNI CAG_nnn'CTG
Cuts at: 174 802 2369 174
Size: 628 1567 1302
Fragments arranged by size:
1567 1302 628
ApaLI G'TGCA_C
Cuts at: 2267 2267
Size: 3497
ApoI r'AATT_y
Cuts at: 320 501 2850 3106 320
Size: 181 2349 256 711
Fragments arranged by size:
2349 711 256 181
AseI AT'TA_AT
Cuts at: 20 20
Size: 3497
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3497
AvaI C'yCGr_G
Cuts at: 517 517
Size: 3497
BamHI G'GATC_C
Cuts at: 522 522
Size: 3497
BanI G'GyrC_C
Cuts at: 513 927 1175 1210 513
Size: 414 248 35 2800
Fragments arranged by size:
2800 414 248 35
BanII G_rGCy'C
Cuts at: 511 1541 511
Size: 1030 2467
BbeI G_GCGC'C
Cuts at: 931 1179 931
Size: 248 3249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 363 397 363
Size: 34 3463
BciVI GTATCCnnnnn_n'
Cuts at: 1395 2156 3497 1395
Size: 761 1341 1395
Fragments arranged by size:
1395 1341 761
BclI T'GATC_A
Cuts at: 1017 1017
Size: 3497
BglII A'GATC_T
Cuts at: 1012 3049 1012
Size: 2037 1460
BlpI GC'TnA_GC
Cuts at: 609 609
Size: 3497
Bme1580I G_kGCm'C
Cuts at: 1122 1215 2271 1122
Size: 93 1056 2348
Fragments arranged by size:
2348 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 709 790 1125 3269 709
Size: 81 335 2144 937
Fragments arranged by size:
2144 937 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1864 1864
Size: 3497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 1552 2044 2342 673
Size: 879 492 298 1828
Fragments arranged by size:
1828 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 144 3201 144
Size: 3057 440
BsaAI yAC'GTr
Cuts at: 850 850
Size: 3497
BsaBI GATnn'nnATC
Cuts at: 6 1011 3048 6
Size: 1005 2037 455
Fragments arranged by size:
2037 1005 455
BsaHI Gr'CG_yC
Cuts at: 928 1176 1878 3374 928
Size: 248 702 1496 1051
Fragments arranged by size:
1496 1051 702 248
BsaWI w'CCGG_w
Cuts at: 686 1207 1885 2159 2306 2717 686
Size: 521 678 274 147 411 1466
Fragments arranged by size:
1466 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 922 1680 2257 2753 922
Size: 758 577 496 1666
Fragments arranged by size:
1666 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1039 1085 2293 104
Size: 935 46 1208 1308
Fragments arranged by size:
1308 1208 935 46
BsiHKAI G_wGCw'C
Cuts at: 511 1289 1479 2271 511
Size: 778 190 792 1737
Fragments arranged by size:
1737 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 511 1122 1215 1289 1479 1541 2271 511
Size: 611 93 74 190 62 730 1737
Fragments arranged by size:
1737 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2717 2717
Size: 3497
BspHI T'CATG_A
Cuts at: 2673 2673
Size: 3497
BspMI ACCTGCnnnn'nnnn_
Cuts at: 159 463 547 761 1063 1444 159
Size: 304 84 214 302 381 2212
Fragments arranged by size:
2212 381 304 302 214 84
BsrBI CCG'CTC
Cuts at: 50 1789 1843 3327 3492 50
Size: 1739 54 1484 165 55
Fragments arranged by size:
1739 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 187 369 1409 187
Size: 182 1040 2275
Fragments arranged by size:
2275 1040 182
BsrFI r'CCGG_y
Cuts at: 1495 1676 1885 1495
Size: 181 209 3107
Fragments arranged by size:
3107 209 181
BsrGI T'GTAC_A
Cuts at: 456 456
Size: 3497
BssSI C'ACGA_G
Cuts at: 1768 2126 1768
Size: 358 3139
BstAPI GCAn_nnn'nTGC
Cuts at: 174 874 174
Size: 700 2797
BstBI TT'CG_AA
Cuts at: 1858 1858
Size: 3497
BstYI r'GATC_y
Cuts at: 1 522 989 1012 1347 1593 2605 2691 2703
Size: 521 467 23 335 246 1012 86 12
Cuts at: 2703 3049 1
Size: 346 449
Fragments arranged by size:
1012 521 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1447 1628 1791 1447
Size: 181 163 3153
Fragments arranged by size:
3153 181 163
BtsI GCAGTG_nn'
Cuts at: 740 740
Size: 3497
DraI TTT'AAA
Cuts at: 496 496
Size: 3497
DrdI GACnn_nn'nnGTC
Cuts at: 1203 2061 1203
Size: 858 2639
EaeI y'GGCC_r
Cuts at: 1082 1256 1647 1674 3019 1082
Size: 174 391 27 1345 1560
Fragments arranged by size:
1560 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1082 1082
Size: 3497
EarI CTCTTCn'nnn_
Cuts at: 1520 1730 1520
Size: 210 3287
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1705 2015 2161 3307 1705
Size: 310 146 1146 1895
Fragments arranged by size:
1895 1146 310 146
EcoICRI GAG'CTC
Cuts at: 509 509
Size: 3497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 1322 1754 2501 2769 421
Size: 901 432 747 268 1149
Fragments arranged by size:
1149 901 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 636 636
Size: 3497
EcoRI G'AATT_C
Cuts at: 320 501 320
Size: 181 3316
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 255 1516 255
Size: 1261 2236
FspI TGC'GCA
Cuts at: 1278 1278
Size: 3497
HaeII r_GCGC'y
Cuts at: 931 1179 2201 3180 931
Size: 248 1022 979 1248
Fragments arranged by size:
1248 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1339 1369 1371 1401 1339
Size: 30 2 30 3435
Fragments arranged by size:
3435 30 30 2
HincII GTy'rAC
Cuts at: 536 536
Size: 3497
KasI G'GCGC_C
Cuts at: 927 1175 927
Size: 248 3249
KpnI G_GTAC'C
Cuts at: 517 517
Size: 3497
MluI A'CGCG_T
Cuts at: 2930 2930
Size: 3497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2168 2352 2168
Size: 184 3313
MscI TGG'CCA
Cuts at: 1258 1258
Size: 3497
MslI CAynn'nnrTG
Cuts at: 1486 1613 1486
Size: 127 3370
MspA1I CmG'CkG
Cuts at: 293 608 922 1282 2295 2540 293
Size: 315 314 360 1013 245 1250
Fragments arranged by size:
1250 1013 360 315 314 245
NaeI GCC'GGC
Cuts at: 1678 1678
Size: 3497
NarI GG'CG_CC
Cuts at: 928 1176 928
Size: 248 3249
NgoMIV G'CCGG_C
Cuts at: 1676 1676
Size: 3497
NspI r_CATG'y
Cuts at: 300 550 1050 1957 300
Size: 250 500 907 1840
Fragments arranged by size:
1840 907 500 250
PciI A'CATG_T
Cuts at: 1953 1953
Size: 3497
PflMI CCAn_nnn'nTGG
Cuts at: 345 345
Size: 3497
PmeI GTTT'AAAC
Cuts at: 496 496
Size: 3497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2636 2668 2636
Size: 32 3465
PstI C_TGCA'G
Cuts at: 544 1229 544
Size: 685 2812
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3497
PvuII CAG'CTG
Cuts at: 922 1282 922
Size: 360 3137
RsrII CG'GwC_CG
Cuts at: 1692 1692
Size: 3497
SacI G_AGCT'C
Cuts at: 511 511
Size: 3497
SalI G'TCGA_C
Cuts at: 534 534
Size: 3497
SapI GCTCTTCn'nnn_
Cuts at: 1520 1730 1520
Size: 210 3287
SbfI CC_TGCA'GG
Cuts at: 544 544
Size: 3497
ScaI AGT'ACT
Cuts at: 3014 3014
Size: 3497
SfcI C'TryA_G
Cuts at: 33 540 1225 2218 2409 33
Size: 507 685 993 191 1121
Fragments arranged by size:
1121 993 685 507 191
SfoI GGC'GCC
Cuts at: 929 1177 929
Size: 248 3249
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3497
SmaI CCC'GGG
Cuts at: 519 519
Size: 3497
SmlI C'TyrA_G
Cuts at: 652 1567 2059 2321 652
Size: 915 492 262 1828
Fragments arranged by size:
1828 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3497
SphI G_CATG'C
Cuts at: 550 1050 550
Size: 500 2997
StyI C'CwwG_G
Cuts at: 631 985 631
Size: 354 3143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1080 1213 1672 1881 1080
Size: 133 459 209 2696
Fragments arranged by size:
2696 459 209 133
TatI w'GTAC_w
Cuts at: 456 3012 456
Size: 2556 941
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 3465
Tth111I GACn'n_nGTC
Cuts at: 1294 1294
Size: 3497
XbaI T'CTAG_A
Cuts at: 264 528 264
Size: 264 3233
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 992 992
Size: 3497
XmaI C'CCGG_G
Cuts at: 517 517
Size: 3497
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI