Restriction map for
pF1KB6698
(Circular) (Six-base) MAPSORT of: pF1KB6698.seq Check: 9570 from: 1 to: 4448
pF1KB6698 4448 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 829 829
Size: 4448
AccI GT'mk_AC
Cuts at: 446 1486 446
Size: 1040 3408
Acc65I G'GTAC_C
Cuts at: 1464 1464
Size: 4448
AclI AA'CG_TT
Cuts at: 4288 4288
Size: 4448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 591 772 1210 2273 2705 3452 3720 345
Size: 246 181 438 1063 432 747 268 1073
Fragments arranged by size:
1073 1063 747 438 432 268 246 181
AflIII A'CryG_T
Cuts at: 251 1800 2904 3881 251
Size: 1549 1104 977 818
Fragments arranged by size:
1549 1104 977 818
AgeI A'CCGG_T
Cuts at: 2836 2836
Size: 4448
AhdI GACnn_n'nnGTC
Cuts at: 1362 1362
Size: 4448
AleI CACnn'nnGTG
Cuts at: 643 643
Size: 4448
AlwNI CAG_nnn'CTG
Cuts at: 1236 1753 3320 1236
Size: 517 1567 2364
Fragments arranged by size:
2364 1567 517
ApaLI G'TGCA_C
Cuts at: 121 3218 121
Size: 3097 1351
ApoI r'AATT_y
Cuts at: 1452 3801 4057 1452
Size: 2349 256 1843
Fragments arranged by size:
2349 1843 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4448
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
AvaI C'yCGr_G
Cuts at: 290 834 967 1468 290
Size: 544 133 501 3270
Fragments arranged by size:
3270 544 501 133
BamHI G'GATC_C
Cuts at: 1139 1473 1139
Size: 334 4114
BanI G'GyrC_C
Cuts at: 157 319 550 879 963 1464 1878 2126 2161
Size: 162 231 329 84 501 414 248 35
Cuts at: 2161 157
Size: 2444
Fragments arranged by size:
2444 501 414 329 248 231 162 84
35
BanII G_rGCy'C
Cuts at: 653 974 1462 2492 653
Size: 321 488 1030 2609
Fragments arranged by size:
2609 1030 488 321
BbeI G_GCGC'C
Cuts at: 161 1882 2130 161
Size: 1721 248 2479
Fragments arranged by size:
2479 1721 248
BbsI GAAGACnn'nnnn_
Cuts at: 1162 1162
Size: 4448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 135 169 1110 1144 90
Size: 34 11 34 941 34 3394
Fragments arranged by size:
3394 941 34 34 34 11
BciVI GTATCCnnnnn_n'
Cuts at: 2346 3107 4448 2346
Size: 761 1341 2346
Fragments arranged by size:
2346 1341 761
BclI T'GATC_A
Cuts at: 1968 1968
Size: 4448
BglI GCCn_nnn'nGGC
Cuts at: 143 143
Size: 4448
BglII A'GATC_T
Cuts at: 356 1963 4000 356
Size: 1607 2037 804
Fragments arranged by size:
2037 1607 804
BlpI GC'TnA_GC
Cuts at: 1560 1560
Size: 4448
Bme1580I G_kGCm'C
Cuts at: 125 553 884 968 2073 2166 3222 125
Size: 428 331 84 1105 93 1056 1351
Fragments arranged by size:
1351 1105 1056 428 331 93 84
BmrI ACTGGGnnnn_n'
Cuts at: 417 1660 1741 2076 4220 417
Size: 1243 81 335 2144 645
Fragments arranged by size:
2144 1243 645 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 331 481 259
Size: 72 150 4226
Fragments arranged by size:
4226 150 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1624 2503 2995 3293 1624
Size: 879 492 298 2779
Fragments arranged by size:
2779 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4152 4152
Size: 4448
BsaAI yAC'GTr
Cuts at: 1801 1801
Size: 4448
BsaBI GATnn'nnATC
Cuts at: 6 1962 3999 6
Size: 1956 2037 455
Fragments arranged by size:
2037 1956 455
BsaHI Gr'CG_yC
Cuts at: 158 1388 1879 2127 2829 4325 158
Size: 1230 491 248 702 1496 281
Fragments arranged by size:
1496 1230 702 491 281 248
BsaWI w'CCGG_w
Cuts at: 1637 2158 2836 3110 3257 3668 1637
Size: 521 678 274 147 411 2417
Fragments arranged by size:
2417 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1432 1435 1438 1432
Size: 3 3 4442
Fragments arranged by size:
4442 3 3
BseYI C'CCAG_C
Cuts at: 977 1873 2631 3208 3704 977
Size: 896 758 577 496 1721
Fragments arranged by size:
1721 896 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1126 1276 880
Size: 246 150 4052
Fragments arranged by size:
4052 246 150
BsiEI CG_ry'CG
Cuts at: 104 1032 1163 1397 1990 2036 3244 104
Size: 928 131 234 593 46 1208 1308
Fragments arranged by size:
1308 1208 928 593 234 131 46
BsiHKAI G_wGCw'C
Cuts at: 125 974 1462 2240 2430 3222 125
Size: 849 488 778 190 792 1351
Fragments arranged by size:
1351 849 792 778 488 190
Bsp1286I G_dGCh'C
Cuts at: 125 553 653 884 968 974 1462 2073 2166
Size: 428 100 231 84 6 488 611 93
Cuts at: 2166 2240 2430 2492 3222 125
Size: 74 190 62 730 1351
Fragments arranged by size:
1351 730 611 488 428 231 190 100
93 84 74 62 6
BspEI T'CCGG_A
Cuts at: 3668 3668
Size: 4448
BspHI T'CATG_A
Cuts at: 596 3624 596
Size: 3028 1420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 829 863 1498 1712 2014 2395 829
Size: 34 635 214 302 381 2882
Fragments arranged by size:
2882 635 381 302 214 34
BsrBI CCG'CTC
Cuts at: 50 809 2740 2794 4278 4443 50
Size: 759 1931 54 1484 165 55
Fragments arranged by size:
1931 1484 759 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2360 2360
Size: 4448
BsrFI r'CCGG_y
Cuts at: 2446 2627 2836 2446
Size: 181 209 4058
Fragments arranged by size:
4058 209 181
BssSI C'ACGA_G
Cuts at: 2719 3077 2719
Size: 358 4090
BstAPI GCAn_nnn'nTGC
Cuts at: 1825 1825
Size: 4448
BstBI TT'CG_AA
Cuts at: 2809 2809
Size: 4448
BstEII G'GTnAC_C
Cuts at: 813 813
Size: 4448
BstXI CCAn_nnnn'nTGG
Cuts at: 303 303
Size: 4448
BstYI r'GATC_y
Cuts at: 1 306 356 569 1139 1473 1940 1963 2298
Size: 305 50 213 570 334 467 23 335
Cuts at: 2298 2544 3556 3642 3654 4000 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 570 467 449 346 335 334 305
246 213 86 50 23 12
BtgI C'CryG_G
Cuts at: 216 323 1026 1182 1391 216
Size: 107 703 156 209 3273
Fragments arranged by size:
3273 703 209 156 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 197 2398 2579 2742 197
Size: 2201 181 163 1903
Fragments arranged by size:
2201 1903 181 163
BtsI GCAGTG_nn'
Cuts at: 1691 1691
Size: 4448
DraI TTT'AAA
Cuts at: 1447 1447
Size: 4448
DraIII CAC_nnn'GTG
Cuts at: 792 792
Size: 4448
DrdI GACnn_nn'nnGTC
Cuts at: 2154 3012 2154
Size: 858 3590
EaeI y'GGCC_r
Cuts at: 351 384 797 1007 1029 1160 1199 1223 1265
Size: 33 413 210 22 131 39 24 42
Cuts at: 1265 1394 2033 2207 2598 2625 3970 351
Size: 129 639 174 391 27 1345 829
Fragments arranged by size:
1345 829 639 413 391 210 174 131
129 42 39 33 27 24 22
EagI C'GGCC_G
Cuts at: 1029 1160 1394 2033 1029
Size: 131 234 639 3444
Fragments arranged by size:
3444 639 234 131
EarI CTCTTCn'nnn_
Cuts at: 572 2471 2681 572
Size: 1899 210 2339
Fragments arranged by size:
2339 1899 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2656 2966 3112 4258 2656
Size: 310 146 1146 2846
Fragments arranged by size:
2846 1146 310 146
EcoICRI GAG'CTC
Cuts at: 972 1460 972
Size: 488 3960
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 331 345 481 591 772 1210 2273 2705
Size: 72 14 136 110 181 438 1063 432
Cuts at: 2705 3452 3720 259
Size: 747 268 987
Fragments arranged by size:
1063 987 747 438 432 268 181 136
110 72 14
EcoO109I rG'GnC_Cy
Cuts at: 723 777 1587 723
Size: 54 810 3584
Fragments arranged by size:
3584 810 54
EcoRI G'AATT_C
Cuts at: 1452 1452
Size: 4448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4448
FspI TGC'GCA
Cuts at: 859 2229 859
Size: 1370 3078
HaeII r_GCGC'y
Cuts at: 161 958 1882 2130 3152 4131 161
Size: 797 924 248 1022 979 478
Fragments arranged by size:
1022 979 924 797 478 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 504 536 1348 1380 2290 2320 2322 2352 504
Size: 32 812 32 910 30 2 30 2600
Fragments arranged by size:
2600 910 812 32 32 30 30 2
HincII GTy'rAC
Cuts at: 447 1487 447
Size: 1040 3408
KasI G'GCGC_C
Cuts at: 157 1878 2126 157
Size: 1721 248 2479
Fragments arranged by size:
2479 1721 248
KpnI G_GTAC'C
Cuts at: 1468 1468
Size: 4448
MluI A'CGCG_T
Cuts at: 3881 3881
Size: 4448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3119 3303 3119
Size: 184 4264
MscI TGG'CCA
Cuts at: 2209 2209
Size: 4448
MslI CAynn'nnrTG
Cuts at: 301 643 1072 1267 2437 2564 301
Size: 342 429 195 1170 127 2185
Fragments arranged by size:
2185 1170 429 342 195 127
MspA1I CmG'CkG
Cuts at: 237 510 681 843 1184 1559 1873 2233 3246
Size: 273 171 162 341 375 314 360 1013
Cuts at: 3246 3491 237
Size: 245 1194
Fragments arranged by size:
1194 1013 375 360 341 314 273 245
171 162
NaeI GCC'GGC
Cuts at: 2629 2629
Size: 4448
NarI GG'CG_CC
Cuts at: 158 1879 2127 158
Size: 1721 248 2479
Fragments arranged by size:
2479 1721 248
NcoI C'CATG_G
Cuts at: 216 323 216
Size: 107 4341
NgoMIV G'CCGG_C
Cuts at: 2627 2627
Size: 4448
NspI r_CATG'y
Cuts at: 1071 1501 2001 2908 1071
Size: 430 500 907 2611
Fragments arranged by size:
2611 907 500 430
PciI A'CATG_T
Cuts at: 2904 2904
Size: 4448
PflMI CCAn_nnn'nTGG
Cuts at: 167 522 681 684 167
Size: 355 159 3 3931
Fragments arranged by size:
3931 355 159 3
PfoI T'CCnGG_A
Cuts at: 455 541 572 1229 455
Size: 86 31 657 3674
Fragments arranged by size:
3674 657 86 31
PmeI GTTT'AAAC
Cuts at: 1447 1447
Size: 4448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1312 1344 3587 3619 1312
Size: 32 2243 32 2141
Fragments arranged by size:
2243 2141 32 32
PpuMI rG'GwC_Cy
Cuts at: 777 777
Size: 4448
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1108 1140 1108
Size: 32 4416
PstI C_TGCA'G
Cuts at: 1495 2180 1495
Size: 685 3763
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4448
PvuII CAG'CTG
Cuts at: 237 510 681 843 1873 2233 237
Size: 273 171 162 1030 360 2452
Fragments arranged by size:
2452 1030 360 273 171 162
RsrII CG'GwC_CG
Cuts at: 2643 2643
Size: 4448
SacI G_AGCT'C
Cuts at: 974 1462 974
Size: 488 3960
SacII CC_GC'GG
Cuts at: 1185 1185
Size: 4448
SalI G'TCGA_C
Cuts at: 445 1485 445
Size: 1040 3408
SapI GCTCTTCn'nnn_
Cuts at: 2471 2681 2471
Size: 210 4238
SbfI CC_TGCA'GG
Cuts at: 1495 1495
Size: 4448
ScaI AGT'ACT
Cuts at: 3965 3965
Size: 4448
SexAI A'CCwGG_T
Cuts at: 788 1358 788
Size: 570 3878
SfcI C'TryA_G
Cuts at: 33 1491 2176 3169 3360 33
Size: 1458 685 993 191 1121
Fragments arranged by size:
1458 1121 993 685 191
SfoI GGC'GCC
Cuts at: 159 1880 2128 159
Size: 1721 248 2479
Fragments arranged by size:
2479 1721 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
SmaI CCC'GGG
Cuts at: 836 1470 836
Size: 634 3814
SmlI C'TyrA_G
Cuts at: 1603 2518 3010 3272 1603
Size: 915 492 262 2779
Fragments arranged by size:
2779 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4448
SphI G_CATG'C
Cuts at: 1501 2001 1501
Size: 500 3948
StuI AGG'CCT
Cuts at: 1285 1285
Size: 4448
StyI C'CwwG_G
Cuts at: 216 323 413 626 1582 1936 216
Size: 107 90 213 956 354 2728
Fragments arranged by size:
2728 956 354 213 107 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 441 639 660 991 2031 2164 2623 2832 441
Size: 198 21 331 1040 133 459 209 2057
Fragments arranged by size:
2057 1040 459 331 209 198 133 21
TatI w'GTAC_w
Cuts at: 254 3963 254
Size: 3709 739
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3965 3997 3965
Size: 32 4416
Tth111I GACn'n_nGTC
Cuts at: 331 451 2245 331
Size: 120 1794 2534
Fragments arranged by size:
2534 1794 120
XbaI T'CTAG_A
Cuts at: 1479 1479
Size: 4448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1002 1080 1943 1002
Size: 78 863 3507
Fragments arranged by size:
3507 863 78
XmaI C'CCGG_G
Cuts at: 834 1468 834
Size: 634 3814
XmnI GAAnn'nnTTC
Cuts at: 604 604
Size: 4448
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI