Restriction map for
pF1KB6695
(Circular) (Six-base) MAPSORT of: pF1KB6695.seq Check: 8001 from: 1 to: 4400
pF1KB6695 4400 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1215 1215
Size: 4400
AccI GT'mk_AC
Cuts at: 1438 1438
Size: 4400
Acc65I G'GTAC_C
Cuts at: 1416 1416
Size: 4400
AclI AA'CG_TT
Cuts at: 4240 4240
Size: 4400
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 1118 2225 2657 3404 3672 469
Size: 649 1107 432 747 268 1197
Fragments arranged by size:
1197 1107 747 649 432 268
AflIII A'CryG_T
Cuts at: 1752 2856 3833 1752
Size: 1104 977 2319
Fragments arranged by size:
2319 1104 977
AgeI A'CCGG_T
Cuts at: 2788 2788
Size: 4400
AhdI GACnn_n'nnGTC
Cuts at: 234 750 234
Size: 516 3884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 566 940 566
Size: 374 4026
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 103
Size: 32 4368
AlwNI CAG_nnn'CTG
Cuts at: 285 1705 3272 285
Size: 1420 1567 1413
Fragments arranged by size:
1567 1420 1413
ApaI G_GGCC'C
Cuts at: 385 1278 385
Size: 893 3507
ApaLI G'TGCA_C
Cuts at: 518 3170 518
Size: 2652 1748
ApoI r'AATT_y
Cuts at: 1404 3753 4009 1404
Size: 2349 256 1795
Fragments arranged by size:
2349 1795 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4400
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4400
AvaI C'yCGr_G
Cuts at: 1420 1420
Size: 4400
BamHI G'GATC_C
Cuts at: 1335 1425 1335
Size: 90 4310
BanI G'GyrC_C
Cuts at: 1227 1416 1830 2078 2113 1227
Size: 189 414 248 35 3514
Fragments arranged by size:
3514 414 248 189 35
BanII G_rGCy'C
Cuts at: 252 385 697 1172 1278 1414 2444 252
Size: 133 312 475 106 136 1030 2208
Fragments arranged by size:
2208 1030 475 312 136 133 106
BbeI G_GCGC'C
Cuts at: 1834 2082 1834
Size: 248 4152
BbsI GAAGACnn'nnnn_
Cuts at: 506 506
Size: 4400
BbvCI CC'TCA_GC
Cuts at: 536 536
Size: 4400
BciVI GTATCCnnnnn_n'
Cuts at: 2298 3059 4400 2298
Size: 761 1341 2298
Fragments arranged by size:
2298 1341 761
BclI T'GATC_A
Cuts at: 833 1920 833
Size: 1087 3313
BfrBI ATG'CAT
Cuts at: 917 917
Size: 4400
BglI GCCn_nnn'nGGC
Cuts at: 1176 1176
Size: 4400
BglII A'GATC_T
Cuts at: 1915 3952 1915
Size: 2037 2363
BlpI GC'TnA_GC
Cuts at: 1512 1512
Size: 4400
Bme1580I G_kGCm'C
Cuts at: 385 522 583 1232 1278 2025 2118 3174 385
Size: 137 61 649 46 747 93 1056 1611
Fragments arranged by size:
1611 1056 747 649 137 93 61 46
BmrI ACTGGGnnnn_n'
Cuts at: 1612 1693 2028 4172 1612
Size: 81 335 2144 1840
Fragments arranged by size:
2144 1840 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 663 2767 663
Size: 2104 2296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 343 380 184
Size: 159 37 4204
Fragments arranged by size:
4204 159 37
Bpu10I CC'TnA_GC
Cuts at: 536 1120 536
Size: 584 3816
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 1327 1576 2455 2947 3245 466
Size: 861 249 879 492 298 1621
Fragments arranged by size:
1621 879 861 492 298 249
BsaI GGTCTCn'nnnn_
Cuts at: 1279 4104 1279
Size: 2825 1575
BsaAI yAC'GTr
Cuts at: 1753 1753
Size: 4400
BsaBI GATnn'nnATC
Cuts at: 6 1914 3951 6
Size: 1908 2037 455
Fragments arranged by size:
2037 1908 455
BsaHI Gr'CG_yC
Cuts at: 1831 2079 2781 4277 1831
Size: 248 702 1496 1954
Fragments arranged by size:
1954 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1589 2110 2788 3062 3209 3620 1589
Size: 521 678 274 147 411 2369
Fragments arranged by size:
2369 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 356 386 512 542 1376 1406 103
Size: 30 223 30 126 30 834 30 3097
Fragments arranged by size:
3097 834 223 126 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 377 552 553 604 1393 377
Size: 175 1 51 789 3384
Fragments arranged by size:
3384 789 175 51 1
BseYI C'CCAG_C
Cuts at: 852 1825 2583 3160 3656 852
Size: 973 758 577 496 1596
Fragments arranged by size:
1596 973 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 1219 162
Size: 1057 3343
BsiEI CG_ry'CG
Cuts at: 104 1942 1988 3196 104
Size: 1838 46 1208 1308
Fragments arranged by size:
1838 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 211 522 1154 1414 2192 2382 3174 211
Size: 311 632 260 778 190 792 1437
Fragments arranged by size:
1437 792 778 632 311 260 190
Bsp1286I G_dGCh'C
Cuts at: 211 252 385 522 583 697 1154 1172 1232
Size: 41 133 137 61 114 457 18 60
Cuts at: 1232 1278 1414 2025 2118 2192 2382 2444 3174
Size: 46 136 611 93 74 190 62 730
Cuts at: 3174 211
Size: 1437
Fragments arranged by size:
1437 730 611 457 190 137 136 133
114 93 74 62 61 60 46 41
18
BspEI T'CCGG_A
Cuts at: 3620 3620
Size: 4400
BspHI T'CATG_A
Cuts at: 3576 3576
Size: 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1215 1450 1664 1966 2347 1215
Size: 235 214 302 381 3268
Fragments arranged by size:
3268 381 302 235 214
BsrBI CCG'CTC
Cuts at: 50 2692 2746 4230 4395 50
Size: 2642 54 1484 165 55
Fragments arranged by size:
2642 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2312 2312
Size: 4400
BsrFI r'CCGG_y
Cuts at: 1220 2398 2579 2788 1220
Size: 1178 181 209 2832
Fragments arranged by size:
2832 1178 209 181
BssSI C'ACGA_G
Cuts at: 2671 3029 2671
Size: 358 4042
BstAPI GCAn_nnn'nTGC
Cuts at: 1032 1777 1032
Size: 745 3655
BstBI TT'CG_AA
Cuts at: 2761 2761
Size: 4400
BstXI CCAn_nnnn'nTGG
Cuts at: 660 660
Size: 4400
BstYI r'GATC_y
Cuts at: 1 282 1335 1425 1892 1915 2250 2496 3508
Size: 281 1053 90 467 23 335 246 1012
Cuts at: 3508 3594 3606 3952 1
Size: 86 12 346 449
Fragments arranged by size:
1053 1012 467 449 346 335 281 246
90 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 473 632 473
Size: 159 4241
BtgI C'CryG_G
Cuts at: 107 659 107
Size: 552 3848
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2350 2531 2694 2350
Size: 181 163 4056
Fragments arranged by size:
4056 181 163
BtsI GCAGTG_nn'
Cuts at: 462 653 1643 462
Size: 191 990 3219
Fragments arranged by size:
3219 990 191
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 253 288 358 393 253
Size: 35 70 35 4260
Fragments arranged by size:
4260 70 35 35
DraI TTT'AAA
Cuts at: 1399 1399
Size: 4400
DrdI GACnn_nn'nnGTC
Cuts at: 2106 2964 2106
Size: 858 3542
EaeI y'GGCC_r
Cuts at: 1985 2159 2550 2577 3922 1985
Size: 174 391 27 1345 2463
Fragments arranged by size:
2463 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1985 1985
Size: 4400
EarI CTCTTCn'nnn_
Cuts at: 166 632 1094 2423 2633 166
Size: 466 462 1329 210 1933
Fragments arranged by size:
1933 1329 466 462 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2608 2918 3064 4210 2608
Size: 310 146 1146 2798
Fragments arranged by size:
2798 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1412 1412
Size: 4400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 343 380 469 1118 2225 2657 3404 3672
Size: 159 37 89 649 1107 432 747 268
Cuts at: 3672 184
Size: 912
Fragments arranged by size:
1107 912 747 649 432 268 159 89
37
EcoNI CCTnn'n_nnAGG
Cuts at: 476 476
Size: 4400
EcoO109I rG'GnC_Cy
Cuts at: 381 382 1274 1539 381
Size: 1 892 265 3242
Fragments arranged by size:
3242 892 265 1
EcoRI G'AATT_C
Cuts at: 1404 1404
Size: 4400
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4400
FspI TGC'GCA
Cuts at: 2181 2181
Size: 4400
HaeII r_GCGC'y
Cuts at: 1834 2082 3104 4083 1834
Size: 248 1022 979 2151
Fragments arranged by size:
2151 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 679 711 736 768 1376 1408 2242
Size: 32 427 32 25 32 608 32 834
Cuts at: 2242 2272 2274 2304 220
Size: 30 2 30 2316
Fragments arranged by size:
2316 834 608 427 32 32 32 32
30 30 25 2
HincII GTy'rAC
Cuts at: 239 1439 239
Size: 1200 3200
KasI G'GCGC_C
Cuts at: 1830 2078 1830
Size: 248 4152
KpnI G_GTAC'C
Cuts at: 1420 1420
Size: 4400
MluI A'CGCG_T
Cuts at: 3833 3833
Size: 4400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3071 3255 3071
Size: 184 4216
MscI TGG'CCA
Cuts at: 2161 2161
Size: 4400
MslI CAynn'nnrTG
Cuts at: 658 940 971 2389 2516 658
Size: 282 31 1418 127 2542
Fragments arranged by size:
2542 1418 282 127 31
MspA1I CmG'CkG
Cuts at: 1511 1825 2185 3198 3443 1511
Size: 314 360 1013 245 2468
Fragments arranged by size:
2468 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2581 2581
Size: 4400
NarI GG'CG_CC
Cuts at: 1831 2079 1831
Size: 248 4152
NcoI C'CATG_G
Cuts at: 107 659 107
Size: 552 3848
NdeI CA'TA_TG
Cuts at: 255 255
Size: 4400
NgoMIV G'CCGG_C
Cuts at: 2579 2579
Size: 4400
NsiI A_TGCA'T
Cuts at: 919 919
Size: 4400
NspI r_CATG'y
Cuts at: 921 1035 1250 1453 1953 2860 921
Size: 114 215 203 500 907 2461
Fragments arranged by size:
2461 907 500 215 203 114
PciI A'CATG_T
Cuts at: 2856 2856
Size: 4400
PmeI GTTT'AAAC
Cuts at: 1399 1399
Size: 4400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 103 135 3539 3571 103
Size: 32 3404 32 932
Fragments arranged by size:
3404 932 32 32
PshAI GACnn'nnGTC
Cuts at: 332 332
Size: 4400
PspOMI G'GGCC_C
Cuts at: 381 1274 381
Size: 893 3507
PstI C_TGCA'G
Cuts at: 1447 2132 1447
Size: 685 3715
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4400
PvuII CAG'CTG
Cuts at: 1825 2185 1825
Size: 360 4040
RsrII CG'GwC_CG
Cuts at: 2595 2595
Size: 4400
SacI G_AGCT'C
Cuts at: 1414 1414
Size: 4400
SalI G'TCGA_C
Cuts at: 1437 1437
Size: 4400
SapI GCTCTTCn'nnn_
Cuts at: 2423 2633 2423
Size: 210 4190
SbfI CC_TGCA'GG
Cuts at: 1447 1447
Size: 4400
ScaI AGT'ACT
Cuts at: 3917 3917
Size: 4400
SexAI A'CCwGG_T
Cuts at: 1211 1211
Size: 4400
SfcI C'TryA_G
Cuts at: 33 268 1443 2128 3121 3312 33
Size: 235 1175 685 993 191 1121
Fragments arranged by size:
1175 1121 993 685 235 191
SfoI GGC'GCC
Cuts at: 1832 2080 1832
Size: 248 4152
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4400
SmaI CCC'GGG
Cuts at: 1422 1422
Size: 4400
SmlI C'TyrA_G
Cuts at: 445 1306 1555 2470 2962 3224 445
Size: 861 249 915 492 262 1621
Fragments arranged by size:
1621 915 861 492 262 249
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4400
SphI G_CATG'C
Cuts at: 921 1035 1250 1453 1953 921
Size: 114 215 203 500 3368
Fragments arranged by size:
3368 500 215 203 114
StuI AGG'CCT
Cuts at: 482 482
Size: 4400
StyI C'CwwG_G
Cuts at: 107 659 1270 1534 1888 107
Size: 552 611 264 354 2619
Fragments arranged by size:
2619 611 552 354 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 406 505 1054 1185 1983 2116 2575 2784 406
Size: 99 549 131 798 133 459 209 2022
Fragments arranged by size:
2022 798 549 459 209 133 131 99
TatI w'GTAC_w
Cuts at: 1310 3915 1310
Size: 2605 1795
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 188 220 356 388 512 544 3917 3949 188
Size: 32 136 32 124 32 3373 32 639
Fragments arranged by size:
3373 639 136 124 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2197 2197
Size: 4400
XbaI T'CTAG_A
Cuts at: 585 1431 585
Size: 846 3554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 269 355 1895 269
Size: 86 1540 2774
Fragments arranged by size:
2774 1540 86
XmaI C'CCGG_G
Cuts at: 1420 1420
Size: 4400
XmnI GAAnn'nnTTC
Cuts at: 482 482
Size: 4400
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PmeI PpiI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AscI AvrII BaeI BcgI
BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI