(Circular) (Six-base) MAPSORT of: pF1KB6691.seq Check: 3975 from: 1 to: 4397 pF1KB6691 4397 bp With 182 enzymes: SgfI * October 7, 2009 14:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 615 615 Size: 4397 AccI GT'mk_AC Cuts at: 1435 1435 Size: 4397 Acc65I G'GTAC_C Cuts at: 1413 1413 Size: 4397 AclI AA'CG_TT Cuts at: 4237 4237 Size: 4397 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 139 443 539 1015 1111 2222 2654 3401 3669 Size: 304 96 476 96 1111 432 747 268 Cuts at: 3669 139 Size: 867 Fragments arranged by size: 1111 867 747 476 432 304 268 96 96 AflIII A'CryG_T Cuts at: 1749 2853 3830 1749 Size: 1104 977 2316 Fragments arranged by size: 2316 1104 977 AgeI A'CCGG_T Cuts at: 2785 2785 Size: 4397 AleI CACnn'nnGTG Cuts at: 728 728 Size: 4397 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1135 1167 1135 Size: 32 4365 AlwNI CAG_nnn'CTG Cuts at: 1023 1702 3269 1023 Size: 679 1567 2151 Fragments arranged by size: 2151 1567 679 ApaI G_GGCC'C Cuts at: 764 764 Size: 4397 ApaLI G'TGCA_C Cuts at: 3167 3167 Size: 4397 ApoI r'AATT_y Cuts at: 1401 3750 4006 1401 Size: 2349 256 1792 Fragments arranged by size: 2349 1792 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4397 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4397 AvaI C'yCGr_G Cuts at: 268 1417 268 Size: 1149 3248 BamHI G'GATC_C Cuts at: 207 1422 207 Size: 1215 3182 BanI G'GyrC_C Cuts at: 1413 1827 2075 2110 1413 Size: 414 248 35 3700 Fragments arranged by size: 3700 414 248 35 BanII G_rGCy'C Cuts at: 764 812 1411 2441 764 Size: 48 599 1030 2720 Fragments arranged by size: 2720 1030 599 48 BbeI G_GCGC'C Cuts at: 1831 2079 1831 Size: 248 4149 BbsI GAAGACnn'nnnn_ Cuts at: 426 426 Size: 4397 BbvCI CC'TCA_GC Cuts at: 865 865 Size: 4397 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1110 1144 1110 Size: 34 4363 BciVI GTATCCnnnnn_n' Cuts at: 2295 3056 4397 2295 Size: 761 1341 2295 Fragments arranged by size: 2295 1341 761 BclI T'GATC_A Cuts at: 1917 1917 Size: 4397 BglII A'GATC_T Cuts at: 1912 3949 1912 Size: 2037 2360 BlpI GC'TnA_GC Cuts at: 1509 1509 Size: 4397 Bme1580I G_kGCm'C Cuts at: 764 2022 2115 3171 764 Size: 1258 93 1056 1990 Fragments arranged by size: 1990 1258 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 698 1609 1690 2025 4169 698 Size: 911 81 335 2144 926 Fragments arranged by size: 2144 926 911 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 211 826 2764 211 Size: 615 1938 1844 Fragments arranged by size: 1938 1844 615 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 386 514 386 Size: 128 4269 Bpu10I CC'TnA_GC Cuts at: 865 865 Size: 4397 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1573 2452 2944 3242 1573 Size: 879 492 298 2728 Fragments arranged by size: 2728 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4101 4101 Size: 4397 BsaAI yAC'GTr Cuts at: 1750 1750 Size: 4397 BsaBI GATnn'nnATC Cuts at: 6 1911 3948 6 Size: 1905 2037 455 Fragments arranged by size: 2037 1905 455 BsaHI Gr'CG_yC Cuts at: 245 1373 1828 2076 2778 4274 245 Size: 1128 455 248 702 1496 368 Fragments arranged by size: 1496 1128 702 455 368 248 BsaWI w'CCGG_w Cuts at: 1586 2107 2785 3059 3206 3617 1586 Size: 521 678 274 147 411 2366 Fragments arranged by size: 2366 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 820 862 820 Size: 42 4355 BseYI C'CCAG_C Cuts at: 1822 2580 3157 3653 1822 Size: 758 577 496 2566 Fragments arranged by size: 2566 758 577 496 BsiEI CG_ry'CG Cuts at: 104 258 1939 1985 3193 104 Size: 154 1681 46 1208 1308 Fragments arranged by size: 1681 1308 1208 154 46 BsiHKAI G_wGCw'C Cuts at: 456 812 1100 1411 2189 2379 3171 456 Size: 356 288 311 778 190 792 1682 Fragments arranged by size: 1682 792 778 356 311 288 190 BsmI GAATG_Cn' Cuts at: 370 1372 370 Size: 1002 3395 Bsp1286I G_dGCh'C Cuts at: 456 764 812 1100 1411 2022 2115 2189 2379 Size: 308 48 288 311 611 93 74 190 Cuts at: 2379 2441 3171 456 Size: 62 730 1682 Fragments arranged by size: 1682 730 611 311 308 288 190 93 74 62 48 BspEI T'CCGG_A Cuts at: 3617 3617 Size: 4397 BspHI T'CATG_A Cuts at: 569 3573 569 Size: 3004 1393 BspMI ACCTGCnnnn'nnnn_ Cuts at: 615 1008 1447 1661 1963 2344 615 Size: 393 439 214 302 381 2668 Fragments arranged by size: 2668 439 393 381 302 214 BsrBI CCG'CTC Cuts at: 50 203 2689 2743 4227 4392 50 Size: 153 2486 54 1484 165 55 Fragments arranged by size: 2486 1484 165 153 55 54 BsrDI GCAATG_nn' Cuts at: 1280 2309 1280 Size: 1029 3368 BsrFI r'CCGG_y Cuts at: 2395 2576 2785 2395 Size: 181 209 4007 Fragments arranged by size: 4007 209 181 BssHII G'CGCG_C Cuts at: 162 162 Size: 4397 BssSI C'ACGA_G Cuts at: 2668 3026 2668 Size: 358 4039 BstAPI GCAn_nnn'nTGC Cuts at: 1774 1774 Size: 4397 BstBI TT'CG_AA Cuts at: 2758 2758 Size: 4397 BstYI r'GATC_y Cuts at: 1 207 1422 1889 1912 2247 2493 3505 3591 Size: 206 1215 467 23 335 246 1012 86 Cuts at: 3591 3603 3949 1 Size: 12 346 449 Fragments arranged by size: 1215 1012 467 449 346 335 246 206 86 23 12 Bsu36I CC'TnA_GG Cuts at: 538 538 Size: 4397 BtgI C'CryG_G Cuts at: 107 1109 1169 107 Size: 1002 60 3335 Fragments arranged by size: 3335 1002 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 303 2347 2528 2691 303 Size: 2044 181 163 2009 Fragments arranged by size: 2044 2009 181 163 BtsI GCAGTG_nn' Cuts at: 1640 1640 Size: 4397 DraI TTT'AAA Cuts at: 1396 1396 Size: 4397 DraIII CAC_nnn'GTG Cuts at: 227 227 Size: 4397 DrdI GACnn_nn'nnGTC Cuts at: 2103 2961 2103 Size: 858 3539 EaeI y'GGCC_r Cuts at: 346 1025 1982 2156 2547 2574 3919 346 Size: 679 957 174 391 27 1345 824 Fragments arranged by size: 1345 957 824 679 391 174 27 EagI C'GGCC_G Cuts at: 1982 1982 Size: 4397 EarI CTCTTCn'nnn_ Cuts at: 202 2420 2630 202 Size: 2218 210 1969 Fragments arranged by size: 2218 1969 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2605 2915 3061 4207 2605 Size: 310 146 1146 2795 Fragments arranged by size: 2795 1146 310 146 EcoICRI GAG'CTC Cuts at: 810 1409 810 Size: 599 3798 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 139 386 443 514 539 1015 1111 2222 2654 Size: 247 57 71 25 476 96 1111 432 Cuts at: 2654 3401 3669 139 Size: 747 268 867 Fragments arranged by size: 1111 867 747 476 432 268 247 96 71 57 25 EcoNI CCTnn'n_nnAGG Cuts at: 1138 1138 Size: 4397 EcoO109I rG'GnC_Cy Cuts at: 534 761 1536 534 Size: 227 775 3395 Fragments arranged by size: 3395 775 227 EcoRI G'AATT_C Cuts at: 1401 1401 Size: 4397 EcoRV GAT'ATC Cuts at: 397 1074 397 Size: 677 3720 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2416 2416 Size: 4397 FspI TGC'GCA Cuts at: 2178 2178 Size: 4397 HaeII r_GCGC'y Cuts at: 855 1831 2079 3101 4080 855 Size: 976 248 1022 979 1172 Fragments arranged by size: 1172 1022 979 976 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 481 513 2239 2269 2271 2301 481 Size: 32 1726 30 2 30 2577 Fragments arranged by size: 2577 1726 32 30 30 2 HincII GTy'rAC Cuts at: 1436 1436 Size: 4397 HindIII A'AGCT_T Cuts at: 341 472 341 Size: 131 4266 KasI G'GCGC_C Cuts at: 1827 2075 1827 Size: 248 4149 KpnI G_GTAC'C Cuts at: 1417 1417 Size: 4397 MluI A'CGCG_T Cuts at: 3830 3830 Size: 4397 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1118 3068 3252 1118 Size: 1950 184 2263 Fragments arranged by size: 2263 1950 184 MscI TGG'CCA Cuts at: 348 1027 2158 348 Size: 679 1131 2587 Fragments arranged by size: 2587 1131 679 MslI CAynn'nnrTG Cuts at: 728 796 2386 2513 728 Size: 68 1590 127 2612 Fragments arranged by size: 2612 1590 127 68 MspA1I CmG'CkG Cuts at: 117 177 260 600 728 864 1089 1508 1822 Size: 60 83 340 128 136 225 419 314 Cuts at: 1822 2182 3195 3440 117 Size: 360 1013 245 1074 Fragments arranged by size: 1074 1013 419 360 340 314 245 225 136 128 83 60 NaeI GCC'GGC Cuts at: 2578 2578 Size: 4397 NarI GG'CG_CC Cuts at: 1828 2076 1828 Size: 248 4149 NcoI C'CATG_G Cuts at: 107 1109 1169 107 Size: 1002 60 3335 Fragments arranged by size: 3335 1002 60 NgoMIV G'CCGG_C Cuts at: 2576 2576 Size: 4397 NspI r_CATG'y Cuts at: 1263 1450 1950 2857 1263 Size: 187 500 907 2803 Fragments arranged by size: 2803 907 500 187 PciI A'CATG_T Cuts at: 2853 2853 Size: 4397 PmeI GTTT'AAAC Cuts at: 1396 1396 Size: 4397 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3536 3568 3536 Size: 32 4365 PspOMI G'GGCC_C Cuts at: 760 760 Size: 4397 PstI C_TGCA'G Cuts at: 1247 1444 2129 1247 Size: 197 685 3515 Fragments arranged by size: 3515 685 197 PvuI CG_AT'CG Cuts at: 104 104 Size: 4397 PvuII CAG'CTG Cuts at: 600 728 864 1089 1822 2182 600 Size: 128 136 225 733 360 2815 Fragments arranged by size: 2815 733 360 225 136 128 RsrII CG'GwC_CG Cuts at: 2592 2592 Size: 4397 SacI G_AGCT'C Cuts at: 812 1411 812 Size: 599 3798 SalI G'TCGA_C Cuts at: 1434 1434 Size: 4397 SapI GCTCTTCn'nnn_ Cuts at: 2420 2630 2420 Size: 210 4187 SbfI CC_TGCA'GG Cuts at: 1444 1444 Size: 4397 ScaI AGT'ACT Cuts at: 877 3914 877 Size: 3037 1360 SfcI C'TryA_G Cuts at: 33 318 1243 1440 2125 3118 3309 33 Size: 285 925 197 685 993 191 1121 Fragments arranged by size: 1121 993 925 685 285 197 191 SfoI GGC'GCC Cuts at: 1829 2077 1829 Size: 248 4149 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4397 SmaI CCC'GGG Cuts at: 1419 1419 Size: 4397 SmlI C'TyrA_G Cuts at: 1552 2467 2959 3221 1552 Size: 915 492 262 2728 Fragments arranged by size: 2728 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4397 SphI G_CATG'C Cuts at: 1450 1950 1450 Size: 500 3897 SspI AAT'ATT Cuts at: 504 504 Size: 4397 StyI C'CwwG_G Cuts at: 107 212 1109 1169 1531 1885 107 Size: 105 897 60 362 354 2619 Fragments arranged by size: 2619 897 362 354 105 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1980 2113 2572 2781 1980 Size: 133 459 209 3596 Fragments arranged by size: 3596 459 209 133 TatI w'GTAC_w Cuts at: 875 3912 875 Size: 3037 1360 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3914 3946 3914 Size: 32 4365 Tth111I GACn'n_nGTC Cuts at: 2194 2194 Size: 4397 XbaI T'CTAG_A Cuts at: 1428 1428 Size: 4397 XcmI CCAnnnn_n'nnnnTGG Cuts at: 822 1892 822 Size: 1070 3327 XmaI C'CCGG_G Cuts at: 1417 1417 Size: 4397 XmnI GAAnn'nnTTC Cuts at: 1064 1064 Size: 4397 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AlfI AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsaXI BsgI BsiWI BsmBI BsrGI BstEII BstXI BstZ17I ClaI CspCI FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI