Restriction map for pF1KB6691
 (Circular) (Six-base) MAPSORT of: pF1KB6691.seq  Check: 3975  from: 1  to: 4397



pF1KB6691  4397 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    615     615 
   Size:       4397
 
AccI GT'mk_AC

Cuts at:   1435    1435 
   Size:       4397
 
Acc65I G'GTAC_C

Cuts at:   1413    1413 
   Size:       4397
 
AclI AA'CG_TT

Cuts at:   4237    4237 
   Size:       4397
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    139     443     539    1015    1111    2222    2654    3401    3669 
   Size:        304      96     476      96    1111     432     747     268

Cuts at:   3669     139 
   Size:        867

  Fragments arranged by size: 

                1111     867     747     476     432     304     268      96
                  96
 
AflIII A'CryG_T

Cuts at:   1749    2853    3830    1749 
   Size:       1104     977    2316

  Fragments arranged by size: 

                2316    1104     977
 
AgeI A'CCGG_T

Cuts at:   2785    2785 
   Size:       4397
 
AleI CACnn'nnGTG

Cuts at:    728     728 
   Size:       4397
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1135    1167    1135 
   Size:         32    4365
 
AlwNI CAG_nnn'CTG

Cuts at:   1023    1702    3269    1023 
   Size:        679    1567    2151

  Fragments arranged by size: 

                2151    1567     679
 
ApaI G_GGCC'C

Cuts at:    764     764 
   Size:       4397
 
ApaLI G'TGCA_C

Cuts at:   3167    3167 
   Size:       4397
 
ApoI r'AATT_y

Cuts at:   1401    3750    4006    1401 
   Size:       2349     256    1792

  Fragments arranged by size: 

                2349    1792     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4397
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4397
 
AvaI C'yCGr_G

Cuts at:    268    1417     268 
   Size:       1149    3248
 
BamHI G'GATC_C

Cuts at:    207    1422     207 
   Size:       1215    3182
 
BanI G'GyrC_C

Cuts at:   1413    1827    2075    2110    1413 
   Size:        414     248      35    3700

  Fragments arranged by size: 

                3700     414     248      35
 
BanII G_rGCy'C

Cuts at:    764     812    1411    2441     764 
   Size:         48     599    1030    2720

  Fragments arranged by size: 

                2720    1030     599      48
 
BbeI G_GCGC'C

Cuts at:   1831    2079    1831 
   Size:        248    4149
 
BbsI GAAGACnn'nnnn_

Cuts at:    426     426 
   Size:       4397
 
BbvCI CC'TCA_GC

Cuts at:    865     865 
   Size:       4397
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1110    1144    1110 
   Size:         34    4363
 
BciVI GTATCCnnnnn_n'

Cuts at:   2295    3056    4397    2295 
   Size:        761    1341    2295

  Fragments arranged by size: 

                2295    1341     761
 
BclI T'GATC_A

Cuts at:   1917    1917 
   Size:       4397
 
BglII A'GATC_T

Cuts at:   1912    3949    1912 
   Size:       2037    2360
 
BlpI GC'TnA_GC

Cuts at:   1509    1509 
   Size:       4397
 
Bme1580I G_kGCm'C

Cuts at:    764    2022    2115    3171     764 
   Size:       1258      93    1056    1990

  Fragments arranged by size: 

                1990    1258    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    698    1609    1690    2025    4169     698 
   Size:        911      81     335    2144     926

  Fragments arranged by size: 

                2144     926     911     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    211     826    2764     211 
   Size:        615    1938    1844

  Fragments arranged by size: 

                1938    1844     615
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    386     514     386 
   Size:        128    4269
 
Bpu10I CC'TnA_GC

Cuts at:    865     865 
   Size:       4397
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1573    2452    2944    3242    1573 
   Size:        879     492     298    2728

  Fragments arranged by size: 

                2728     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4101    4101 
   Size:       4397
 
BsaAI yAC'GTr

Cuts at:   1750    1750 
   Size:       4397
 
BsaBI GATnn'nnATC

Cuts at:      6    1911    3948       6 
   Size:       1905    2037     455

  Fragments arranged by size: 

                2037    1905     455
 
BsaHI Gr'CG_yC

Cuts at:    245    1373    1828    2076    2778    4274     245 
   Size:       1128     455     248     702    1496     368

  Fragments arranged by size: 

                1496    1128     702     455     368     248
 
BsaWI w'CCGG_w

Cuts at:   1586    2107    2785    3059    3206    3617    1586 
   Size:        521     678     274     147     411    2366

  Fragments arranged by size: 

                2366     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    820     862     820 
   Size:         42    4355
 
BseYI C'CCAG_C

Cuts at:   1822    2580    3157    3653    1822 
   Size:        758     577     496    2566

  Fragments arranged by size: 

                2566     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     258    1939    1985    3193     104 
   Size:        154    1681      46    1208    1308

  Fragments arranged by size: 

                1681    1308    1208     154      46
 
BsiHKAI G_wGCw'C

Cuts at:    456     812    1100    1411    2189    2379    3171     456 
   Size:        356     288     311     778     190     792    1682

  Fragments arranged by size: 

                1682     792     778     356     311     288     190
 
BsmI GAATG_Cn'

Cuts at:    370    1372     370 
   Size:       1002    3395
 
Bsp1286I G_dGCh'C

Cuts at:    456     764     812    1100    1411    2022    2115    2189    2379 
   Size:        308      48     288     311     611      93      74     190

Cuts at:   2379    2441    3171     456 
   Size:         62     730    1682

  Fragments arranged by size: 

                1682     730     611     311     308     288     190      93
                  74      62      48
 
BspEI T'CCGG_A

Cuts at:   3617    3617 
   Size:       4397
 
BspHI T'CATG_A

Cuts at:    569    3573     569 
   Size:       3004    1393
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    615    1008    1447    1661    1963    2344     615 
   Size:        393     439     214     302     381    2668

  Fragments arranged by size: 

                2668     439     393     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     203    2689    2743    4227    4392      50 
   Size:        153    2486      54    1484     165      55

  Fragments arranged by size: 

                2486    1484     165     153      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1280    2309    1280 
   Size:       1029    3368
 
BsrFI r'CCGG_y

Cuts at:   2395    2576    2785    2395 
   Size:        181     209    4007

  Fragments arranged by size: 

                4007     209     181
 
BssHII G'CGCG_C

Cuts at:    162     162 
   Size:       4397
 
BssSI C'ACGA_G

Cuts at:   2668    3026    2668 
   Size:        358    4039
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1774    1774 
   Size:       4397
 
BstBI TT'CG_AA

Cuts at:   2758    2758 
   Size:       4397
 
BstYI r'GATC_y

Cuts at:      1     207    1422    1889    1912    2247    2493    3505    3591 
   Size:        206    1215     467      23     335     246    1012      86

Cuts at:   3591    3603    3949       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1215    1012     467     449     346     335     246     206
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    538     538 
   Size:       4397
 
BtgI C'CryG_G

Cuts at:    107    1109    1169     107 
   Size:       1002      60    3335

  Fragments arranged by size: 

                3335    1002      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    303    2347    2528    2691     303 
   Size:       2044     181     163    2009

  Fragments arranged by size: 

                2044    2009     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1640    1640 
   Size:       4397
 
DraI TTT'AAA

Cuts at:   1396    1396 
   Size:       4397
 
DraIII CAC_nnn'GTG

Cuts at:    227     227 
   Size:       4397
 
DrdI GACnn_nn'nnGTC

Cuts at:   2103    2961    2103 
   Size:        858    3539
 
EaeI y'GGCC_r

Cuts at:    346    1025    1982    2156    2547    2574    3919     346 
   Size:        679     957     174     391      27    1345     824

  Fragments arranged by size: 

                1345     957     824     679     391     174      27
 
EagI C'GGCC_G

Cuts at:   1982    1982 
   Size:       4397
 
EarI CTCTTCn'nnn_

Cuts at:    202    2420    2630     202 
   Size:       2218     210    1969

  Fragments arranged by size: 

                2218    1969     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2605    2915    3061    4207    2605 
   Size:        310     146    1146    2795

  Fragments arranged by size: 

                2795    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    810    1409     810 
   Size:        599    3798
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    139     386     443     514     539    1015    1111    2222    2654 
   Size:        247      57      71      25     476      96    1111     432

Cuts at:   2654    3401    3669     139 
   Size:        747     268     867

  Fragments arranged by size: 

                1111     867     747     476     432     268     247      96
                  71      57      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1138    1138 
   Size:       4397
 
EcoO109I rG'GnC_Cy

Cuts at:    534     761    1536     534 
   Size:        227     775    3395

  Fragments arranged by size: 

                3395     775     227
 
EcoRI G'AATT_C

Cuts at:   1401    1401 
   Size:       4397
 
EcoRV GAT'ATC

Cuts at:    397    1074     397 
   Size:        677    3720
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2416    2416 
   Size:       4397
 
FspI TGC'GCA

Cuts at:   2178    2178 
   Size:       4397
 
HaeII r_GCGC'y

Cuts at:    855    1831    2079    3101    4080     855 
   Size:        976     248    1022     979    1172

  Fragments arranged by size: 

                1172    1022     979     976     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    481     513    2239    2269    2271    2301     481 
   Size:         32    1726      30       2      30    2577

  Fragments arranged by size: 

                2577    1726      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1436    1436 
   Size:       4397
 
HindIII A'AGCT_T

Cuts at:    341     472     341 
   Size:        131    4266
 
KasI G'GCGC_C

Cuts at:   1827    2075    1827 
   Size:        248    4149
 
KpnI G_GTAC'C

Cuts at:   1417    1417 
   Size:       4397
 
MluI A'CGCG_T

Cuts at:   3830    3830 
   Size:       4397
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1118    3068    3252    1118 
   Size:       1950     184    2263

  Fragments arranged by size: 

                2263    1950     184
 
MscI TGG'CCA

Cuts at:    348    1027    2158     348 
   Size:        679    1131    2587

  Fragments arranged by size: 

                2587    1131     679
 
MslI CAynn'nnrTG

Cuts at:    728     796    2386    2513     728 
   Size:         68    1590     127    2612

  Fragments arranged by size: 

                2612    1590     127      68
 
MspA1I CmG'CkG

Cuts at:    117     177     260     600     728     864    1089    1508    1822 
   Size:         60      83     340     128     136     225     419     314

Cuts at:   1822    2182    3195    3440     117 
   Size:        360    1013     245    1074

  Fragments arranged by size: 

                1074    1013     419     360     340     314     245     225
                 136     128      83      60
 
NaeI GCC'GGC

Cuts at:   2578    2578 
   Size:       4397
 
NarI GG'CG_CC

Cuts at:   1828    2076    1828 
   Size:        248    4149
 
NcoI C'CATG_G

Cuts at:    107    1109    1169     107 
   Size:       1002      60    3335

  Fragments arranged by size: 

                3335    1002      60
 
NgoMIV G'CCGG_C

Cuts at:   2576    2576 
   Size:       4397
 
NspI r_CATG'y

Cuts at:   1263    1450    1950    2857    1263 
   Size:        187     500     907    2803

  Fragments arranged by size: 

                2803     907     500     187
 
PciI A'CATG_T

Cuts at:   2853    2853 
   Size:       4397
 
PmeI GTTT'AAAC

Cuts at:   1396    1396 
   Size:       4397
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3536    3568    3536 
   Size:         32    4365
 
PspOMI G'GGCC_C

Cuts at:    760     760 
   Size:       4397
 
PstI C_TGCA'G

Cuts at:   1247    1444    2129    1247 
   Size:        197     685    3515

  Fragments arranged by size: 

                3515     685     197
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4397
 
PvuII CAG'CTG

Cuts at:    600     728     864    1089    1822    2182     600 
   Size:        128     136     225     733     360    2815

  Fragments arranged by size: 

                2815     733     360     225     136     128
 
RsrII CG'GwC_CG

Cuts at:   2592    2592 
   Size:       4397
 
SacI G_AGCT'C

Cuts at:    812    1411     812 
   Size:        599    3798
 
SalI G'TCGA_C

Cuts at:   1434    1434 
   Size:       4397
 
SapI GCTCTTCn'nnn_

Cuts at:   2420    2630    2420 
   Size:        210    4187
 
SbfI CC_TGCA'GG

Cuts at:   1444    1444 
   Size:       4397
 
ScaI AGT'ACT

Cuts at:    877    3914     877 
   Size:       3037    1360
 
SfcI C'TryA_G

Cuts at:     33     318    1243    1440    2125    3118    3309      33 
   Size:        285     925     197     685     993     191    1121

  Fragments arranged by size: 

                1121     993     925     685     285     197     191
 
SfoI GGC'GCC

Cuts at:   1829    2077    1829 
   Size:        248    4149
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4397
 
SmaI CCC'GGG

Cuts at:   1419    1419 
   Size:       4397
 
SmlI C'TyrA_G

Cuts at:   1552    2467    2959    3221    1552 
   Size:        915     492     262    2728

  Fragments arranged by size: 

                2728     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4397
 
SphI G_CATG'C

Cuts at:   1450    1950    1450 
   Size:        500    3897
 
SspI AAT'ATT

Cuts at:    504     504 
   Size:       4397
 
StyI C'CwwG_G

Cuts at:    107     212    1109    1169    1531    1885     107 
   Size:        105     897      60     362     354    2619

  Fragments arranged by size: 

                2619     897     362     354     105      60
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1980    2113    2572    2781    1980 
   Size:        133     459     209    3596

  Fragments arranged by size: 

                3596     459     209     133
 
TatI w'GTAC_w

Cuts at:    875    3912     875 
   Size:       3037    1360
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3914    3946    3914 
   Size:         32    4365
 
Tth111I GACn'n_nGTC

Cuts at:   2194    2194 
   Size:       4397
 
XbaI T'CTAG_A

Cuts at:   1428    1428 
   Size:       4397
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    822    1892     822 
   Size:       1070    3327
 
XmaI C'CCGG_G

Cuts at:   1417    1417 
   Size:       4397
 
XmnI GAAnn'nnTTC

Cuts at:   1064    1064 
   Size:       4397
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI     PmeI     PpiI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AlfI     AscI    AvrII     BaeI
    BfrBI     BglI    BmgBI     BmtI    BsaXI     BsgI    BsiWI    BsmBI
    BsrGI   BstEII    BstXI  BstZ17I     ClaI    CspCI     FseI    FspAI
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     StuI     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com