Restriction map for
pF1KB6691
(Circular) (Six-base) MAPSORT of: pF1KB6691.seq Check: 3975 from: 1 to: 4397
pF1KB6691 4397 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 615 615
Size: 4397
AccI GT'mk_AC
Cuts at: 1435 1435
Size: 4397
Acc65I G'GTAC_C
Cuts at: 1413 1413
Size: 4397
AclI AA'CG_TT
Cuts at: 4237 4237
Size: 4397
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 443 539 1015 1111 2222 2654 3401 3669
Size: 304 96 476 96 1111 432 747 268
Cuts at: 3669 139
Size: 867
Fragments arranged by size:
1111 867 747 476 432 304 268 96
96
AflIII A'CryG_T
Cuts at: 1749 2853 3830 1749
Size: 1104 977 2316
Fragments arranged by size:
2316 1104 977
AgeI A'CCGG_T
Cuts at: 2785 2785
Size: 4397
AleI CACnn'nnGTG
Cuts at: 728 728
Size: 4397
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1135 1167 1135
Size: 32 4365
AlwNI CAG_nnn'CTG
Cuts at: 1023 1702 3269 1023
Size: 679 1567 2151
Fragments arranged by size:
2151 1567 679
ApaI G_GGCC'C
Cuts at: 764 764
Size: 4397
ApaLI G'TGCA_C
Cuts at: 3167 3167
Size: 4397
ApoI r'AATT_y
Cuts at: 1401 3750 4006 1401
Size: 2349 256 1792
Fragments arranged by size:
2349 1792 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4397
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4397
AvaI C'yCGr_G
Cuts at: 268 1417 268
Size: 1149 3248
BamHI G'GATC_C
Cuts at: 207 1422 207
Size: 1215 3182
BanI G'GyrC_C
Cuts at: 1413 1827 2075 2110 1413
Size: 414 248 35 3700
Fragments arranged by size:
3700 414 248 35
BanII G_rGCy'C
Cuts at: 764 812 1411 2441 764
Size: 48 599 1030 2720
Fragments arranged by size:
2720 1030 599 48
BbeI G_GCGC'C
Cuts at: 1831 2079 1831
Size: 248 4149
BbsI GAAGACnn'nnnn_
Cuts at: 426 426
Size: 4397
BbvCI CC'TCA_GC
Cuts at: 865 865
Size: 4397
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1110 1144 1110
Size: 34 4363
BciVI GTATCCnnnnn_n'
Cuts at: 2295 3056 4397 2295
Size: 761 1341 2295
Fragments arranged by size:
2295 1341 761
BclI T'GATC_A
Cuts at: 1917 1917
Size: 4397
BglII A'GATC_T
Cuts at: 1912 3949 1912
Size: 2037 2360
BlpI GC'TnA_GC
Cuts at: 1509 1509
Size: 4397
Bme1580I G_kGCm'C
Cuts at: 764 2022 2115 3171 764
Size: 1258 93 1056 1990
Fragments arranged by size:
1990 1258 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 698 1609 1690 2025 4169 698
Size: 911 81 335 2144 926
Fragments arranged by size:
2144 926 911 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 211 826 2764 211
Size: 615 1938 1844
Fragments arranged by size:
1938 1844 615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 514 386
Size: 128 4269
Bpu10I CC'TnA_GC
Cuts at: 865 865
Size: 4397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1573 2452 2944 3242 1573
Size: 879 492 298 2728
Fragments arranged by size:
2728 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4101 4101
Size: 4397
BsaAI yAC'GTr
Cuts at: 1750 1750
Size: 4397
BsaBI GATnn'nnATC
Cuts at: 6 1911 3948 6
Size: 1905 2037 455
Fragments arranged by size:
2037 1905 455
BsaHI Gr'CG_yC
Cuts at: 245 1373 1828 2076 2778 4274 245
Size: 1128 455 248 702 1496 368
Fragments arranged by size:
1496 1128 702 455 368 248
BsaWI w'CCGG_w
Cuts at: 1586 2107 2785 3059 3206 3617 1586
Size: 521 678 274 147 411 2366
Fragments arranged by size:
2366 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 820 862 820
Size: 42 4355
BseYI C'CCAG_C
Cuts at: 1822 2580 3157 3653 1822
Size: 758 577 496 2566
Fragments arranged by size:
2566 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 258 1939 1985 3193 104
Size: 154 1681 46 1208 1308
Fragments arranged by size:
1681 1308 1208 154 46
BsiHKAI G_wGCw'C
Cuts at: 456 812 1100 1411 2189 2379 3171 456
Size: 356 288 311 778 190 792 1682
Fragments arranged by size:
1682 792 778 356 311 288 190
BsmI GAATG_Cn'
Cuts at: 370 1372 370
Size: 1002 3395
Bsp1286I G_dGCh'C
Cuts at: 456 764 812 1100 1411 2022 2115 2189 2379
Size: 308 48 288 311 611 93 74 190
Cuts at: 2379 2441 3171 456
Size: 62 730 1682
Fragments arranged by size:
1682 730 611 311 308 288 190 93
74 62 48
BspEI T'CCGG_A
Cuts at: 3617 3617
Size: 4397
BspHI T'CATG_A
Cuts at: 569 3573 569
Size: 3004 1393
BspMI ACCTGCnnnn'nnnn_
Cuts at: 615 1008 1447 1661 1963 2344 615
Size: 393 439 214 302 381 2668
Fragments arranged by size:
2668 439 393 381 302 214
BsrBI CCG'CTC
Cuts at: 50 203 2689 2743 4227 4392 50
Size: 153 2486 54 1484 165 55
Fragments arranged by size:
2486 1484 165 153 55 54
BsrDI GCAATG_nn'
Cuts at: 1280 2309 1280
Size: 1029 3368
BsrFI r'CCGG_y
Cuts at: 2395 2576 2785 2395
Size: 181 209 4007
Fragments arranged by size:
4007 209 181
BssHII G'CGCG_C
Cuts at: 162 162
Size: 4397
BssSI C'ACGA_G
Cuts at: 2668 3026 2668
Size: 358 4039
BstAPI GCAn_nnn'nTGC
Cuts at: 1774 1774
Size: 4397
BstBI TT'CG_AA
Cuts at: 2758 2758
Size: 4397
BstYI r'GATC_y
Cuts at: 1 207 1422 1889 1912 2247 2493 3505 3591
Size: 206 1215 467 23 335 246 1012 86
Cuts at: 3591 3603 3949 1
Size: 12 346 449
Fragments arranged by size:
1215 1012 467 449 346 335 246 206
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 538 538
Size: 4397
BtgI C'CryG_G
Cuts at: 107 1109 1169 107
Size: 1002 60 3335
Fragments arranged by size:
3335 1002 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 303 2347 2528 2691 303
Size: 2044 181 163 2009
Fragments arranged by size:
2044 2009 181 163
BtsI GCAGTG_nn'
Cuts at: 1640 1640
Size: 4397
DraI TTT'AAA
Cuts at: 1396 1396
Size: 4397
DraIII CAC_nnn'GTG
Cuts at: 227 227
Size: 4397
DrdI GACnn_nn'nnGTC
Cuts at: 2103 2961 2103
Size: 858 3539
EaeI y'GGCC_r
Cuts at: 346 1025 1982 2156 2547 2574 3919 346
Size: 679 957 174 391 27 1345 824
Fragments arranged by size:
1345 957 824 679 391 174 27
EagI C'GGCC_G
Cuts at: 1982 1982
Size: 4397
EarI CTCTTCn'nnn_
Cuts at: 202 2420 2630 202
Size: 2218 210 1969
Fragments arranged by size:
2218 1969 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2605 2915 3061 4207 2605
Size: 310 146 1146 2795
Fragments arranged by size:
2795 1146 310 146
EcoICRI GAG'CTC
Cuts at: 810 1409 810
Size: 599 3798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 386 443 514 539 1015 1111 2222 2654
Size: 247 57 71 25 476 96 1111 432
Cuts at: 2654 3401 3669 139
Size: 747 268 867
Fragments arranged by size:
1111 867 747 476 432 268 247 96
71 57 25
EcoNI CCTnn'n_nnAGG
Cuts at: 1138 1138
Size: 4397
EcoO109I rG'GnC_Cy
Cuts at: 534 761 1536 534
Size: 227 775 3395
Fragments arranged by size:
3395 775 227
EcoRI G'AATT_C
Cuts at: 1401 1401
Size: 4397
EcoRV GAT'ATC
Cuts at: 397 1074 397
Size: 677 3720
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4397
FspI TGC'GCA
Cuts at: 2178 2178
Size: 4397
HaeII r_GCGC'y
Cuts at: 855 1831 2079 3101 4080 855
Size: 976 248 1022 979 1172
Fragments arranged by size:
1172 1022 979 976 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 481 513 2239 2269 2271 2301 481
Size: 32 1726 30 2 30 2577
Fragments arranged by size:
2577 1726 32 30 30 2
HincII GTy'rAC
Cuts at: 1436 1436
Size: 4397
HindIII A'AGCT_T
Cuts at: 341 472 341
Size: 131 4266
KasI G'GCGC_C
Cuts at: 1827 2075 1827
Size: 248 4149
KpnI G_GTAC'C
Cuts at: 1417 1417
Size: 4397
MluI A'CGCG_T
Cuts at: 3830 3830
Size: 4397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1118 3068 3252 1118
Size: 1950 184 2263
Fragments arranged by size:
2263 1950 184
MscI TGG'CCA
Cuts at: 348 1027 2158 348
Size: 679 1131 2587
Fragments arranged by size:
2587 1131 679
MslI CAynn'nnrTG
Cuts at: 728 796 2386 2513 728
Size: 68 1590 127 2612
Fragments arranged by size:
2612 1590 127 68
MspA1I CmG'CkG
Cuts at: 117 177 260 600 728 864 1089 1508 1822
Size: 60 83 340 128 136 225 419 314
Cuts at: 1822 2182 3195 3440 117
Size: 360 1013 245 1074
Fragments arranged by size:
1074 1013 419 360 340 314 245 225
136 128 83 60
NaeI GCC'GGC
Cuts at: 2578 2578
Size: 4397
NarI GG'CG_CC
Cuts at: 1828 2076 1828
Size: 248 4149
NcoI C'CATG_G
Cuts at: 107 1109 1169 107
Size: 1002 60 3335
Fragments arranged by size:
3335 1002 60
NgoMIV G'CCGG_C
Cuts at: 2576 2576
Size: 4397
NspI r_CATG'y
Cuts at: 1263 1450 1950 2857 1263
Size: 187 500 907 2803
Fragments arranged by size:
2803 907 500 187
PciI A'CATG_T
Cuts at: 2853 2853
Size: 4397
PmeI GTTT'AAAC
Cuts at: 1396 1396
Size: 4397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 4365
PspOMI G'GGCC_C
Cuts at: 760 760
Size: 4397
PstI C_TGCA'G
Cuts at: 1247 1444 2129 1247
Size: 197 685 3515
Fragments arranged by size:
3515 685 197
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4397
PvuII CAG'CTG
Cuts at: 600 728 864 1089 1822 2182 600
Size: 128 136 225 733 360 2815
Fragments arranged by size:
2815 733 360 225 136 128
RsrII CG'GwC_CG
Cuts at: 2592 2592
Size: 4397
SacI G_AGCT'C
Cuts at: 812 1411 812
Size: 599 3798
SalI G'TCGA_C
Cuts at: 1434 1434
Size: 4397
SapI GCTCTTCn'nnn_
Cuts at: 2420 2630 2420
Size: 210 4187
SbfI CC_TGCA'GG
Cuts at: 1444 1444
Size: 4397
ScaI AGT'ACT
Cuts at: 877 3914 877
Size: 3037 1360
SfcI C'TryA_G
Cuts at: 33 318 1243 1440 2125 3118 3309 33
Size: 285 925 197 685 993 191 1121
Fragments arranged by size:
1121 993 925 685 285 197 191
SfoI GGC'GCC
Cuts at: 1829 2077 1829
Size: 248 4149
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4397
SmaI CCC'GGG
Cuts at: 1419 1419
Size: 4397
SmlI C'TyrA_G
Cuts at: 1552 2467 2959 3221 1552
Size: 915 492 262 2728
Fragments arranged by size:
2728 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4397
SphI G_CATG'C
Cuts at: 1450 1950 1450
Size: 500 3897
SspI AAT'ATT
Cuts at: 504 504
Size: 4397
StyI C'CwwG_G
Cuts at: 107 212 1109 1169 1531 1885 107
Size: 105 897 60 362 354 2619
Fragments arranged by size:
2619 897 362 354 105 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1980 2113 2572 2781 1980
Size: 133 459 209 3596
Fragments arranged by size:
3596 459 209 133
TatI w'GTAC_w
Cuts at: 875 3912 875
Size: 3037 1360
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3914 3946 3914
Size: 32 4365
Tth111I GACn'n_nGTC
Cuts at: 2194 2194
Size: 4397
XbaI T'CTAG_A
Cuts at: 1428 1428
Size: 4397
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 822 1892 822
Size: 1070 3327
XmaI C'CCGG_G
Cuts at: 1417 1417
Size: 4397
XmnI GAAnn'nnTTC
Cuts at: 1064 1064
Size: 4397
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BfrBI BglI BmgBI BmtI BsaXI BsgI BsiWI BsmBI
BsrGI BstEII BstXI BstZ17I ClaI CspCI FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI