Restriction map for
pF1KB6686
(Circular) (Six-base) MAPSORT of: pF1KB6686.seq Check: 5541 from: 1 to: 4487
pF1KB6686 4487 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 1030 1525 1030
Size: 495 3992
Acc65I G'GTAC_C
Cuts at: 1503 1503
Size: 4487
AclI AA'CG_TT
Cuts at: 4327 4327
Size: 4487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 660 2312 2744 3491 3759 550
Size: 110 1652 432 747 268 1278
Fragments arranged by size:
1652 1278 747 432 268 110
AflIII A'CryG_T
Cuts at: 1294 1839 2943 3920 1294
Size: 545 1104 977 1861
Fragments arranged by size:
1861 1104 977 545
AgeI A'CCGG_T
Cuts at: 2875 2875
Size: 4487
AlwNI CAG_nnn'CTG
Cuts at: 1792 3359 1792
Size: 1567 2920
ApaLI G'TGCA_C
Cuts at: 3257 3257
Size: 4487
ApoI r'AATT_y
Cuts at: 853 1491 3840 4096 853
Size: 638 2349 256 1244
Fragments arranged by size:
2349 1244 638 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4487
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
AvaI C'yCGr_G
Cuts at: 1333 1507 1333
Size: 174 4313
BamHI G'GATC_C
Cuts at: 1512 1512
Size: 4487
BanI G'GyrC_C
Cuts at: 524 1503 1917 2165 2200 524
Size: 979 414 248 35 2811
Fragments arranged by size:
2811 979 414 248 35
BanII G_rGCy'C
Cuts at: 130 541 593 761 1079 1238 1501 2531 130
Size: 411 52 168 318 159 263 1030 2086
Fragments arranged by size:
2086 1030 411 318 263 168 159 52
BbeI G_GCGC'C
Cuts at: 1921 2169 1921
Size: 248 4239
BbvCI CC'TCA_GC
Cuts at: 983 1371 983
Size: 388 4099
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1314 1348 1314
Size: 34 4453
BciVI GTATCCnnnnn_n'
Cuts at: 2385 3146 4487 2385
Size: 761 1341 2385
Fragments arranged by size:
2385 1341 761
BclI T'GATC_A
Cuts at: 2007 2007
Size: 4487
BglI GCCn_nnn'nGGC
Cuts at: 661 661
Size: 4487
BglII A'GATC_T
Cuts at: 2002 4039 2002
Size: 2037 2450
BlpI GC'TnA_GC
Cuts at: 1123 1228 1599 1123
Size: 105 371 4011
Fragments arranged by size:
4011 371 105
Bme1580I G_kGCm'C
Cuts at: 2112 2205 3261 2112
Size: 93 1056 3338
Fragments arranged by size:
3338 1056 93
BmgBI CAC'GTC
Cuts at: 1297 1297
Size: 4487
BmrI ACTGGGnnnn_n'
Cuts at: 1699 1780 2115 4259 1699
Size: 81 335 2144 1927
Fragments arranged by size:
2144 1927 335 81
BmtI G_CTAG'C
Cuts at: 548 817 548
Size: 269 4218
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 4487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 502 523 661 802 1120 1162 1321 484
Size: 18 21 138 141 318 42 159 3650
Fragments arranged by size:
3650 318 159 141 138 42 21 18
Bpu10I CC'TnA_GC
Cuts at: 551 983 1078 1371 551
Size: 432 95 293 3667
Fragments arranged by size:
3667 432 293 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 1663 2542 3034 3332 667
Size: 996 879 492 298 1822
Fragments arranged by size:
1822 996 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4191 4191
Size: 4487
BsaAI yAC'GTr
Cuts at: 1840 1840
Size: 4487
BsaBI GATnn'nnATC
Cuts at: 6 790 2001 4038 6
Size: 784 1211 2037 455
Fragments arranged by size:
2037 1211 784 455
BsaHI Gr'CG_yC
Cuts at: 1346 1918 2166 2868 4364 1346
Size: 572 248 702 1496 1469
Fragments arranged by size:
1496 1469 702 572 248
BsaWI w'CCGG_w
Cuts at: 1676 2197 2875 3149 3296 3707 1676
Size: 521 678 274 147 411 2456
Fragments arranged by size:
2456 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 236 266 236
Size: 30 4457
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 120 194 394 499 856 120
Size: 74 200 105 357 3751
Fragments arranged by size:
3751 357 200 105 74
BseYI C'CCAG_C
Cuts at: 174 232 540 1118 1223 1912 2670 3247 3743
Size: 58 308 578 105 689 758 577 496
Cuts at: 3743 174
Size: 918
Fragments arranged by size:
918 758 689 578 577 496 308 105
58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1028 1028
Size: 4487
BsiEI CG_ry'CG
Cuts at: 104 447 2029 2075 3283 104
Size: 343 1582 46 1208 1308
Fragments arranged by size:
1582 1308 1208 343 46
BsiHKAI G_wGCw'C
Cuts at: 130 593 761 1079 1238 1501 2279 2469 3261
Size: 463 168 318 159 263 778 190 792
Cuts at: 3261 130
Size: 1356
Fragments arranged by size:
1356 792 778 463 318 263 190 168
159
BsmBI CGTCTCn'nnnn_
Cuts at: 1153 1153
Size: 4487
Bsp1286I G_dGCh'C
Cuts at: 130 541 593 761 1079 1238 1501 2112 2205
Size: 411 52 168 318 159 263 611 93
Cuts at: 2205 2279 2469 2531 3261 130
Size: 74 190 62 730 1356
Fragments arranged by size:
1356 730 611 411 318 263 190 168
159 93 74 62 52
BspEI T'CCGG_A
Cuts at: 3707 3707
Size: 4487
BspHI T'CATG_A
Cuts at: 3663 3663
Size: 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1537 1751 2053 2434 1537
Size: 214 302 381 3590
Fragments arranged by size:
3590 381 302 214
BsrBI CCG'CTC
Cuts at: 50 555 2779 2833 4317 4482 50
Size: 505 2224 54 1484 165 55
Fragments arranged by size:
2224 1484 505 165 55 54
BsrDI GCAATG_nn'
Cuts at: 420 2399 420
Size: 1979 2508
BsrFI r'CCGG_y
Cuts at: 2485 2666 2875 2485
Size: 181 209 4097
Fragments arranged by size:
4097 209 181
BssHII G'CGCG_C
Cuts at: 474 474
Size: 4487
BssSI C'ACGA_G
Cuts at: 2758 3116 2758
Size: 358 4129
BstAPI GCAn_nnn'nTGC
Cuts at: 1864 1864
Size: 4487
BstBI TT'CG_AA
Cuts at: 2848 2848
Size: 4487
BstYI r'GATC_y
Cuts at: 1 512 602 1193 1512 1979 2002 2337 2583
Size: 511 90 591 319 467 23 335 246
Cuts at: 2583 3595 3681 3693 4039 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 591 511 467 449 346 335 319
246 90 86 23 12
BtgI C'CryG_G
Cuts at: 186 956 186
Size: 770 3717
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2437 2618 2781 2437
Size: 181 163 4143
Fragments arranged by size:
4143 181 163
BtsI GCAGTG_nn'
Cuts at: 1037 1248 1730 1037
Size: 211 482 3794
Fragments arranged by size:
3794 482 211
DraI TTT'AAA
Cuts at: 1486 1486
Size: 4487
DrdI GACnn_nn'nnGTC
Cuts at: 2193 3051 2193
Size: 858 3629
EaeI y'GGCC_r
Cuts at: 873 2072 2246 2637 2664 4009 873
Size: 1199 174 391 27 1345 1351
Fragments arranged by size:
1351 1345 1199 391 174 27
EagI C'GGCC_G
Cuts at: 2072 2072
Size: 4487
EarI CTCTTCn'nnn_
Cuts at: 581 803 833 2510 2720 581
Size: 222 30 1677 210 2348
Fragments arranged by size:
2348 1677 222 210 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 106 2695 3005 3151 4297 106
Size: 2589 310 146 1146 296
Fragments arranged by size:
2589 1146 310 296 146
EcoICRI GAG'CTC
Cuts at: 128 591 759 1077 1236 1499 128
Size: 463 168 318 159 263 3116
Fragments arranged by size:
3116 463 318 263 168 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 502 523 550 660 661 802 1120 1162
Size: 18 21 27 110 1 141 318 42
Cuts at: 1162 1321 2312 2744 3491 3759 484
Size: 159 991 432 747 268 1212
Fragments arranged by size:
1212 991 747 432 318 268 159 141
110 42 27 21 18 1
EcoNI CCTnn'n_nnAGG
Cuts at: 466 484 1333 466
Size: 18 849 3620
Fragments arranged by size:
3620 849 18
EcoO109I rG'GnC_Cy
Cuts at: 1626 1626
Size: 4487
EcoRI G'AATT_C
Cuts at: 1491 1491
Size: 4487
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4487
FspI TGC'GCA
Cuts at: 2268 2268
Size: 4487
HaeII r_GCGC'y
Cuts at: 1921 2169 3191 4170 1921
Size: 248 1022 979 2238
Fragments arranged by size:
2238 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1099 1131 2329 2359 2361 2391 1099
Size: 32 1198 30 2 30 3195
Fragments arranged by size:
3195 1198 32 30 30 2
HincII GTy'rAC
Cuts at: 378 882 1526 378
Size: 504 644 3339
Fragments arranged by size:
3339 644 504
KasI G'GCGC_C
Cuts at: 1917 2165 1917
Size: 248 4239
KpnI G_GTAC'C
Cuts at: 1507 1507
Size: 4487
MluI A'CGCG_T
Cuts at: 3920 3920
Size: 4487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3158 3342 3158
Size: 184 4303
MscI TGG'CCA
Cuts at: 2248 2248
Size: 4487
MslI CAynn'nnrTG
Cuts at: 1042 2476 2603 1042
Size: 1434 127 2926
Fragments arranged by size:
2926 1434 127
MspA1I CmG'CkG
Cuts at: 174 232 1122 1185 1227 1598 1912 2272 3285
Size: 58 890 63 42 371 314 360 1013
Cuts at: 3285 3530 174
Size: 245 1131
Fragments arranged by size:
1131 1013 890 371 360 314 245 63
58 42
NaeI GCC'GGC
Cuts at: 2668 2668
Size: 4487
NarI GG'CG_CC
Cuts at: 1918 2166 1918
Size: 248 4239
NcoI C'CATG_G
Cuts at: 956 956
Size: 4487
NgoMIV G'CCGG_C
Cuts at: 2666 2666
Size: 4487
NheI G'CTAG_C
Cuts at: 544 813 544
Size: 269 4218
NspI r_CATG'y
Cuts at: 1540 2040 2947 1540
Size: 500 907 3080
Fragments arranged by size:
3080 907 500
PciI A'CATG_T
Cuts at: 2943 2943
Size: 4487
PflMI CCAn_nnn'nTGG
Cuts at: 717 1098 717
Size: 381 4106
PfoI T'CCnGG_A
Cuts at: 593 638 593
Size: 45 4442
PmeI GTTT'AAAC
Cuts at: 1486 1486
Size: 4487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 976 1008 3626 3658 976
Size: 32 2618 32 1805
Fragments arranged by size:
2618 1805 32 32
PspXI vC'TCGA_Gb
Cuts at: 1333 1333
Size: 4487
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1252 1284 1252
Size: 32 4455
PstI C_TGCA'G
Cuts at: 125 1115 1184 1190 1534 2219 125
Size: 990 69 6 344 685 2393
Fragments arranged by size:
2393 990 685 344 69 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4487
PvuII CAG'CTG
Cuts at: 174 232 1122 1185 1227 1912 2272 174
Size: 58 890 63 42 685 360 2389
Fragments arranged by size:
2389 890 685 360 63 58 42
RsrII CG'GwC_CG
Cuts at: 2682 2682
Size: 4487
SacI G_AGCT'C
Cuts at: 130 593 761 1079 1238 1501 130
Size: 463 168 318 159 263 3116
Fragments arranged by size:
3116 463 318 263 168 159
SalI G'TCGA_C
Cuts at: 1524 1524
Size: 4487
SapI GCTCTTCn'nnn_
Cuts at: 581 803 2510 2720 581
Size: 222 1707 210 2348
Fragments arranged by size:
2348 1707 222 210
SbfI CC_TGCA'GG
Cuts at: 1534 1534
Size: 4487
ScaI AGT'ACT
Cuts at: 4004 4004
Size: 4487
SexAI A'CCwGG_T
Cuts at: 863 863
Size: 4487
SfcI C'TryA_G
Cuts at: 33 121 561 1111 1180 1186 1530 2215 3208
Size: 88 440 550 69 6 344 685 993
Cuts at: 3208 3399 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 550 440 344 191 88
69 6
SfoI GGC'GCC
Cuts at: 1919 2167 1919
Size: 248 4239
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
SmaI CCC'GGG
Cuts at: 1509 1509
Size: 4487
SmlI C'TyrA_G
Cuts at: 682 1333 1642 2557 3049 3311 682
Size: 651 309 915 492 262 1858
Fragments arranged by size:
1858 915 651 492 309 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4487
SphI G_CATG'C
Cuts at: 1540 2040 1540
Size: 500 3987
StuI AGG'CCT
Cuts at: 1097 1479 1097
Size: 382 4105
StyI C'CwwG_G
Cuts at: 312 336 956 1092 1621 1975 312
Size: 24 620 136 529 354 2824
Fragments arranged by size:
2824 620 529 354 136 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2070 2203 2662 2871 2070
Size: 133 459 209 3686
Fragments arranged by size:
3686 459 209 133
TatI w'GTAC_w
Cuts at: 1268 4002 1268
Size: 2734 1753
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 236 268 4004 4036 236
Size: 32 3736 32 687
Fragments arranged by size:
3736 687 32 32
Tth111I GACn'n_nGTC
Cuts at: 2284 2284
Size: 4487
XbaI T'CTAG_A
Cuts at: 1518 1518
Size: 4487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 498 663 1982 498
Size: 165 1319 3003
Fragments arranged by size:
3003 1319 165
XhoI C'TCGA_G
Cuts at: 1333 1333
Size: 4487
XmaI C'CCGG_G
Cuts at: 1507 1507
Size: 4487
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PflMI PfoI PmeI PpiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BfrBI BsiWI BsmI
BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI