Restriction map for
pF1KB6680
(Circular) (Six-base) MAPSORT of: pF1KB6680.seq Check: 8696 from: 1 to: 4481
pF1KB6680 4481 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 1519 1519
Size: 4481
Acc65I G'GTAC_C
Cuts at: 527 1497 527
Size: 970 3511
AclI AA'CG_TT
Cuts at: 4321 4321
Size: 4481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 663 847 984 1370 2306 2738 3485 3753
Size: 412 184 137 386 936 432 747 268
Cuts at: 3753 251
Size: 979
Fragments arranged by size:
979 936 747 432 412 386 268 184
137
AflIII A'CryG_T
Cuts at: 1833 2937 3914 1833
Size: 1104 977 2400
Fragments arranged by size:
2400 1104 977
AgeI A'CCGG_T
Cuts at: 2869 2869
Size: 4481
AlwNI CAG_nnn'CTG
Cuts at: 1101 1786 3353 1101
Size: 685 1567 2229
Fragments arranged by size:
2229 1567 685
ApaLI G'TGCA_C
Cuts at: 3251 3251
Size: 4481
ApoI r'AATT_y
Cuts at: 1485 3834 4090 1485
Size: 2349 256 1876
Fragments arranged by size:
2349 1876 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4481
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4481
AvaI C'yCGr_G
Cuts at: 161 632 1336 1501 161
Size: 471 704 165 3141
Fragments arranged by size:
3141 704 471 165
BamHI G'GATC_C
Cuts at: 1506 1506
Size: 4481
BanI G'GyrC_C
Cuts at: 527 1497 1911 2159 2194 527
Size: 970 414 248 35 2814
Fragments arranged by size:
2814 970 414 248 35
BanII G_rGCy'C
Cuts at: 596 1208 1241 1495 2525 596
Size: 612 33 254 1030 2552
Fragments arranged by size:
2552 1030 612 254 33
BbeI G_GCGC'C
Cuts at: 1915 2163 1915
Size: 248 4233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1317 1351 1317
Size: 34 4447
BciVI GTATCCnnnnn_n'
Cuts at: 2379 3140 4481 2379
Size: 761 1341 2379
Fragments arranged by size:
2379 1341 761
BclI T'GATC_A
Cuts at: 2001 2001
Size: 4481
BglI GCCn_nnn'nGGC
Cuts at: 664 1357 664
Size: 693 3788
BglII A'GATC_T
Cuts at: 1996 4033 1996
Size: 2037 2444
BlpI GC'TnA_GC
Cuts at: 1126 1593 1126
Size: 467 4014
Bme1580I G_kGCm'C
Cuts at: 483 2106 2199 3255 483
Size: 1623 93 1056 1709
Fragments arranged by size:
1709 1623 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1693 1774 2109 4253 1693
Size: 81 335 2144 1921
Fragments arranged by size:
2144 1921 335 81
BmtI G_CTAG'C
Cuts at: 820 820
Size: 4481
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1050 2848 1050
Size: 1798 2683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 526 664 805 1123 1165 1240 1324 505
Size: 21 138 141 318 42 75 84 3662
Fragments arranged by size:
3662 318 141 138 84 75 42 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 1657 2536 3028 3326 670
Size: 987 879 492 298 1825
Fragments arranged by size:
1825 987 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 409 4185 409
Size: 3776 705
BsaAI yAC'GTr
Cuts at: 478 1834 478
Size: 1356 3125
BsaBI GATnn'nnATC
Cuts at: 6 793 1995 4032 6
Size: 787 1202 2037 455
Fragments arranged by size:
2037 1202 787 455
BsaHI Gr'CG_yC
Cuts at: 1912 2160 2862 4358 1912
Size: 248 702 1496 2035
Fragments arranged by size:
2035 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1670 2191 2869 3143 3290 3701 1670
Size: 521 678 274 147 411 2450
Fragments arranged by size:
2450 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 344 374 344
Size: 30 4451
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 293 397 502 826 859 1027 293
Size: 104 105 324 33 168 3747
Fragments arranged by size:
3747 324 168 105 104 33
BseYI C'CCAG_C
Cuts at: 181 543 1121 1138 1226 1906 2664 3241 3737
Size: 362 578 17 88 680 758 577 496
Cuts at: 3737 181
Size: 925
Fragments arranged by size:
925 758 680 578 577 496 362 88
17
BsiEI CG_ry'CG
Cuts at: 104 2023 2069 3277 104
Size: 1919 46 1208 1308
Fragments arranged by size:
1919 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 596 1241 1495 2273 2463 3255 596
Size: 645 254 778 190 792 1822
Fragments arranged by size:
1822 792 778 645 254 190
BsmI GAATG_Cn'
Cuts at: 562 562
Size: 4481
BsmBI CGTCTCn'nnnn_
Cuts at: 1077 1077
Size: 4481
Bsp1286I G_dGCh'C
Cuts at: 483 596 1208 1241 1495 2106 2199 2273 2463
Size: 113 612 33 254 611 93 74 190
Cuts at: 2463 2525 3255 483
Size: 62 730 1709
Fragments arranged by size:
1709 730 612 611 254 190 113 93
74 62 33
BspEI T'CCGG_A
Cuts at: 3701 3701
Size: 4481
BspHI T'CATG_A
Cuts at: 3657 3657
Size: 4481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1531 1745 2047 2428 1531
Size: 214 302 381 3584
Fragments arranged by size:
3584 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2773 2827 4311 4476 50
Size: 2723 54 1484 165 55
Fragments arranged by size:
2723 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 423 2393 423
Size: 1970 2511
BsrFI r'CCGG_y
Cuts at: 874 2479 2660 2869 874
Size: 1605 181 209 2486
Fragments arranged by size:
2486 1605 209 181
BssSI C'ACGA_G
Cuts at: 2752 3110 2752
Size: 358 4123
BstAPI GCAn_nnn'nTGC
Cuts at: 1858 1858
Size: 4481
BstBI TT'CG_AA
Cuts at: 2842 2842
Size: 4481
BstYI r'GATC_y
Cuts at: 1 515 1196 1506 1973 1996 2331 2577 3589
Size: 514 681 310 467 23 335 246 1012
Cuts at: 3589 3675 3687 4033 1
Size: 86 12 346 449
Fragments arranged by size:
1012 681 514 467 449 346 335 310
246 86 23 12
BtgI C'CryG_G
Cuts at: 959 959
Size: 4481
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 410 2431 2612 2775 410
Size: 2021 181 163 2116
Fragments arranged by size:
2116 2021 181 163
BtsI GCAGTG_nn'
Cuts at: 1724 1724
Size: 4481
DraI TTT'AAA
Cuts at: 1480 1480
Size: 4481
DraIII CAC_nnn'GTG
Cuts at: 910 910
Size: 4481
DrdI GACnn_nn'nnGTC
Cuts at: 2187 3045 2187
Size: 858 3623
EaeI y'GGCC_r
Cuts at: 611 770 872 876 1157 2066 2240 2631 2658
Size: 159 102 4 281 909 174 391 27
Cuts at: 2658 4003 611
Size: 1345 1089
Fragments arranged by size:
1345 1089 909 391 281 174 159 102
27 4
EagI C'GGCC_G
Cuts at: 2066 2066
Size: 4481
EarI CTCTTCn'nnn_
Cuts at: 270 584 836 1389 1424 2504 2714 270
Size: 314 252 553 35 1080 210 2037
Fragments arranged by size:
2037 1080 553 314 252 210 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 979 2689 2999 3145 4291 979
Size: 1710 310 146 1146 1169
Fragments arranged by size:
1710 1169 1146 310 146
EcoICRI GAG'CTC
Cuts at: 594 1239 1493 594
Size: 645 254 3582
Fragments arranged by size:
3582 645 254
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 505 526 663 664 805 847 984 1123
Size: 254 21 137 1 141 42 137 139
Cuts at: 1123 1165 1240 1324 1370 2306 2738 3485 3753
Size: 42 75 84 46 936 432 747 268
Cuts at: 3753 251
Size: 979
Fragments arranged by size:
979 936 747 432 268 254 141 139
137 137 84 75 46 42 42 21
1
EcoNI CCTnn'n_nnAGG
Cuts at: 469 487 712 808 1336 469
Size: 18 225 96 528 3614
Fragments arranged by size:
3614 528 225 96 18
EcoO109I rG'GnC_Cy
Cuts at: 801 1620 801
Size: 819 3662
EcoRI G'AATT_C
Cuts at: 1485 1485
Size: 4481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 4481
FseI GG_CCGG'CC
Cuts at: 878 878
Size: 4481
FspI TGC'GCA
Cuts at: 2262 2262
Size: 4481
HaeII r_GCGC'y
Cuts at: 1915 2163 3185 4164 1915
Size: 248 1022 979 2232
Fragments arranged by size:
2232 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1102 1134 1242 1274 2323 2353 2355 2385 1102
Size: 32 108 32 1049 30 2 30 3198
Fragments arranged by size:
3198 1049 108 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1520 1520
Size: 4481
KasI G'GCGC_C
Cuts at: 1911 2159 1911
Size: 248 4233
KpnI G_GTAC'C
Cuts at: 531 1501 531
Size: 970 3511
MluI A'CGCG_T
Cuts at: 3914 3914
Size: 4481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1061 3152 3336 1061
Size: 2091 184 2206
Fragments arranged by size:
2206 2091 184
MscI TGG'CCA
Cuts at: 613 772 2242 613
Size: 159 1470 2852
Fragments arranged by size:
2852 1470 159
MslI CAynn'nnrTG
Cuts at: 1045 1421 2470 2597 1045
Size: 376 1049 127 2929
Fragments arranged by size:
2929 1049 376 127
MspA1I CmG'CkG
Cuts at: 870 1188 1230 1592 1906 2266 3279 3524 870
Size: 318 42 362 314 360 1013 245 1827
Fragments arranged by size:
1827 1013 362 360 318 314 245 42
NaeI GCC'GGC
Cuts at: 876 2662 876
Size: 1786 2695
NarI GG'CG_CC
Cuts at: 1912 2160 1912
Size: 248 4233
NcoI C'CATG_G
Cuts at: 959 959
Size: 4481
NgoMIV G'CCGG_C
Cuts at: 874 2660 874
Size: 1786 2695
NheI G'CTAG_C
Cuts at: 816 816
Size: 4481
NspI r_CATG'y
Cuts at: 1534 2034 2941 1534
Size: 500 907 3074
Fragments arranged by size:
3074 907 500
PciI A'CATG_T
Cuts at: 2937 2937
Size: 4481
PfoI T'CCnGG_A
Cuts at: 596 641 752 596
Size: 45 111 4325
Fragments arranged by size:
4325 111 45
PmeI GTTT'AAAC
Cuts at: 1480 1480
Size: 4481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 979 1011 3620 3652 979
Size: 32 2609 32 1808
Fragments arranged by size:
2609 1808 32 32
PspXI vC'TCGA_Gb
Cuts at: 1336 1336
Size: 4481
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1255 1287 1255
Size: 32 4449
PstI C_TGCA'G
Cuts at: 1118 1193 1528 2213 1118
Size: 75 335 685 3386
Fragments arranged by size:
3386 685 335 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4481
PvuII CAG'CTG
Cuts at: 870 1188 1230 1906 2266 870
Size: 318 42 676 360 3085
Fragments arranged by size:
3085 676 360 318 42
RsrII CG'GwC_CG
Cuts at: 2676 2676
Size: 4481
SacI G_AGCT'C
Cuts at: 596 1241 1495 596
Size: 645 254 3582
Fragments arranged by size:
3582 645 254
SalI G'TCGA_C
Cuts at: 1518 1518
Size: 4481
SapI GCTCTTCn'nnn_
Cuts at: 584 2504 2714 584
Size: 1920 210 2351
Fragments arranged by size:
2351 1920 210
SbfI CC_TGCA'GG
Cuts at: 1528 1528
Size: 4481
ScaI AGT'ACT
Cuts at: 3998 3998
Size: 4481
SfcI C'TryA_G
Cuts at: 33 564 1114 1189 1524 2209 3202 3393 33
Size: 531 550 75 335 685 993 191 1121
Fragments arranged by size:
1121 993 685 550 531 335 191 75
SfoI GGC'GCC
Cuts at: 1913 2161 1913
Size: 248 4233
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4481
SmaI CCC'GGG
Cuts at: 634 1503 634
Size: 869 3612
SmlI C'TyrA_G
Cuts at: 685 1336 1636 2551 3043 3305 685
Size: 651 300 915 492 262 1861
Fragments arranged by size:
1861 915 651 492 300 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4481
SphI G_CATG'C
Cuts at: 1534 2034 1534
Size: 500 3981
StuI AGG'CCT
Cuts at: 991 991
Size: 4481
StyI C'CwwG_G
Cuts at: 959 1615 1969 959
Size: 656 354 3471
Fragments arranged by size:
3471 656 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2064 2197 2656 2865 2064
Size: 133 459 209 3680
Fragments arranged by size:
3680 459 209 133
TatI w'GTAC_w
Cuts at: 1271 3996 1271
Size: 2725 1756
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3998 4030 3998
Size: 32 4449
Tth111I GACn'n_nGTC
Cuts at: 2278 2278
Size: 4481
XbaI T'CTAG_A
Cuts at: 1512 1512
Size: 4481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 501 780 1207 1976 501
Size: 279 427 769 3006
Fragments arranged by size:
3006 769 427 279
XhoI C'TCGA_G
Cuts at: 1336 1336
Size: 4481
XmaI C'CCGG_G
Cuts at: 632 1501 632
Size: 869 3612
XmnI GAAnn'nnTTC
Cuts at: 856 856
Size: 4481
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PciI PfoI PmeI
PpiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI
Bpu10I BsgI BsiWI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoRV FspAI HindIII HpaI MfeI
NdeI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI ZraI