Restriction map for
pF1KB6675
(Circular) (Six-base) MAPSORT of: pF1KB6675.seq Check: 487 from: 1 to: 4394
pF1KB6675 4394 bp
With 182 enzymes: SgfI *
March 22, 2013 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 106 784 106
Size: 678 3716
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2219 2651 3398 3666 2219
Size: 432 747 268 2947
Fragments arranged by size:
2947 747 432 268
AflIII A'CryG_T
Cuts at: 1746 2850 3827 1746
Size: 1104 977 2313
Fragments arranged by size:
2313 1104 977
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AleI CACnn'nnGTG
Cuts at: 227 227
Size: 4394
AlwNI CAG_nnn'CTG
Cuts at: 1047 1699 3266 1047
Size: 652 1567 2175
Fragments arranged by size:
2175 1567 652
ApaLI G'TGCA_C
Cuts at: 363 645 3164 363
Size: 282 2519 1593
Fragments arranged by size:
2519 1593 282
ApoI r'AATT_y
Cuts at: 1398 3747 4003 1398
Size: 2349 256 1789
Fragments arranged by size:
2349 1789 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 501 839 1080 1216 1414 501
Size: 338 241 136 198 3481
Fragments arranged by size:
3481 338 241 198 136
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 118 187 396 756 844 1187 1410 1824 2072
Size: 69 209 360 88 343 223 414 248
Cuts at: 2072 2107 118
Size: 35 2405
Fragments arranged by size:
2405 414 360 343 248 223 209 88
69 35
BanII G_rGCy'C
Cuts at: 305 383 502 721 1408 2438 305
Size: 78 119 219 687 1030 2261
Fragments arranged by size:
2261 1030 687 219 119 78
BbeI G_GCGC'C
Cuts at: 400 1828 2076 400
Size: 1428 248 2718
Fragments arranged by size:
2718 1428 248
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BglI GCCn_nnn'nGGC
Cuts at: 112 196 209 560 1312 112
Size: 84 13 351 752 3194
Fragments arranged by size:
3194 752 351 84 13
BglII A'GATC_T
Cuts at: 1909 3946 1909
Size: 2037 2357
BlpI GC'TnA_GC
Cuts at: 1506 1506
Size: 4394
Bme1580I G_kGCm'C
Cuts at: 367 556 649 2019 2112 3168 367
Size: 189 93 1370 93 1056 1593
Fragments arranged by size:
1593 1370 1056 189 93 93
BmgBI CAC'GTC
Cuts at: 333 333
Size: 4394
BmrI ACTGGGnnnn_n'
Cuts at: 473 1606 1687 2022 4166 473
Size: 1133 81 335 2144 701
Fragments arranged by size:
2144 1133 701 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 516 1024 1360 2761 516
Size: 508 336 1401 2149
Fragments arranged by size:
2149 1401 508 336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 746 367
Size: 379 4015
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1570 2449 2941 3239 1078
Size: 492 879 492 298 2233
Fragments arranged by size:
2233 879 492 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4098 4098
Size: 4394
BsaAI yAC'GTr
Cuts at: 566 588 1747 566
Size: 22 1159 3213
Fragments arranged by size:
3213 1159 22
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 397 419 662 985 1304 1825 2073 2775 4271
Size: 22 243 323 319 521 248 702 1496
Cuts at: 4271 397
Size: 520
Fragments arranged by size:
1496 702 521 520 323 319 248 243
22
BsaWI w'CCGG_w
Cuts at: 1583 2104 2782 3056 3203 3614 1583
Size: 521 678 274 147 411 2363
Fragments arranged by size:
2363 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1213 1243 1342 1372 1213
Size: 30 99 30 4235
Fragments arranged by size:
4235 99 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 562 688 1156 562
Size: 126 468 3800
Fragments arranged by size:
3800 468 126
BseYI C'CCAG_C
Cuts at: 269 653 736 824 1128 1203 1274 1819 2577
Size: 384 83 88 304 75 71 545 758
Cuts at: 2577 3154 3650 269
Size: 577 496 1013
Fragments arranged by size:
1013 758 577 545 496 384 304 88
83 75 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 591 1380 591
Size: 789 3605
BsiEI CG_ry'CG
Cuts at: 104 129 347 1936 1982 3190 104
Size: 25 218 1589 46 1208 1308
Fragments arranged by size:
1589 1308 1208 218 46 25
BsiHKAI G_wGCw'C
Cuts at: 367 649 1223 1408 2186 2376 3168 367
Size: 282 574 185 778 190 792 1593
Fragments arranged by size:
1593 792 778 574 282 190 185
BsmBI CGTCTCn'nnnn_
Cuts at: 451 451
Size: 4394
Bsp1286I G_dGCh'C
Cuts at: 305 367 383 502 556 649 721 1223 1408
Size: 62 16 119 54 93 72 502 185
Cuts at: 1408 2019 2112 2186 2376 2438 3168 305
Size: 611 93 74 190 62 730 1531
Fragments arranged by size:
1531 730 611 502 190 185 119 93
93 74 72 62 62 54 16
BspEI T'CCGG_A
Cuts at: 3614 3614
Size: 4394
BspHI T'CATG_A
Cuts at: 3570 3570
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 106 784 1444 1658 1960 2341 106
Size: 678 660 214 302 381 2159
Fragments arranged by size:
2159 678 660 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2306 2306
Size: 4394
BsrFI r'CCGG_y
Cuts at: 124 968 2392 2573 2782 124
Size: 844 1424 181 209 1736
Fragments arranged by size:
1736 1424 844 209 181
BsrGI T'GTAC_A
Cuts at: 215 215
Size: 4394
BssSI C'ACGA_G
Cuts at: 508 1345 2665 3023 508
Size: 837 1320 358 1879
Fragments arranged by size:
1879 1320 837 358
BstAPI GCAn_nnn'nTGC
Cuts at: 245 362 371 614 1771 245
Size: 117 9 243 1157 2868
Fragments arranged by size:
2868 1157 243 117 9
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstEII G'GTnAC_C
Cuts at: 801 834 801
Size: 33 4361
BstXI CCAn_nnnn'nTGG
Cuts at: 277 588 789 813 277
Size: 311 201 24 3858
Fragments arranged by size:
3858 311 201 24
BstYI r'GATC_y
Cuts at: 1 677 1419 1886 1909 2244 2490 3502 3588
Size: 676 742 467 23 335 246 1012 86
Cuts at: 3588 3600 3946 1
Size: 12 346 449
Fragments arranged by size:
1012 742 676 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 752 752
Size: 4394
BtgI C'CryG_G
Cuts at: 107 134 830 1031 107
Size: 27 696 201 3470
Fragments arranged by size:
3470 696 201 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 1368 1370 1637 1368
Size: 2 267 4125
Fragments arranged by size:
4125 267 2
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DraIII CAC_nnn'GTG
Cuts at: 371 542 614 371
Size: 171 72 4151
Fragments arranged by size:
4151 171 72
DrdI GACnn_nn'nnGTC
Cuts at: 2100 2958 2100
Size: 858 3536
EaeI y'GGCC_r
Cuts at: 126 684 970 1313 1979 2153 2544 2571 3916
Size: 558 286 343 666 174 391 27 1345
Cuts at: 3916 126
Size: 604
Fragments arranged by size:
1345 666 604 558 391 343 286 174
27
EagI C'GGCC_G
Cuts at: 126 1979 126
Size: 1853 2541
EarI CTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 2912 3058 4204 2602
Size: 310 146 1146 2792
Fragments arranged by size:
2792 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1406 1406
Size: 4394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 746 2219 2651 3398 3666 367
Size: 379 1473 432 747 268 1095
Fragments arranged by size:
1473 1095 747 432 379 268
EcoNI CCTnn'n_nnAGG
Cuts at: 256 924 256
Size: 668 3726
EcoO109I rG'GnC_Cy
Cuts at: 1089 1533 1089
Size: 444 3950
EcoRI G'AATT_C
Cuts at: 1398 1398
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4394
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 400 471 633 1295 1828 2076 3098 4077 400
Size: 71 162 662 533 248 1022 979 717
Fragments arranged by size:
1022 979 717 662 533 248 162 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2236 2266 2268 2298 2236
Size: 30 2 30 4332
Fragments arranged by size:
4332 30 30 2
HincII GTy'rAC
Cuts at: 1433 1433
Size: 4394
HindIII A'AGCT_T
Cuts at: 1153 1153
Size: 4394
KasI G'GCGC_C
Cuts at: 396 1824 2072 396
Size: 1428 248 2718
Fragments arranged by size:
2718 1428 248
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 353 596 670 3065 3249 353
Size: 243 74 2395 184 1498
Fragments arranged by size:
2395 1498 243 184 74
MscI TGG'CCA
Cuts at: 2155 2155
Size: 4394
MslI CAynn'nnrTG
Cuts at: 227 811 1030 2383 2510 227
Size: 584 219 1353 127 2111
Fragments arranged by size:
2111 1353 584 219 127
MspA1I CmG'CkG
Cuts at: 227 431 938 1505 1819 2179 3192 3437 227
Size: 204 507 567 314 360 1013 245 1184
Fragments arranged by size:
1184 1013 567 507 360 314 245 204
NaeI GCC'GGC
Cuts at: 970 2575 970
Size: 1605 2789
NarI GG'CG_CC
Cuts at: 397 1825 2073 397
Size: 1428 248 2718
Fragments arranged by size:
2718 1428 248
NcoI C'CATG_G
Cuts at: 107 134 107
Size: 27 4367
NgoMIV G'CCGG_C
Cuts at: 968 2573 968
Size: 1605 2789
NspI r_CATG'y
Cuts at: 203 393 1447 1947 2854 203
Size: 190 1054 500 907 1743
Fragments arranged by size:
1743 1054 907 500 190
PciI A'CATG_T
Cuts at: 2850 2850
Size: 4394
PflMI CCAn_nnn'nTGG
Cuts at: 831 831
Size: 4394
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PmlI CAC'GTG
Cuts at: 588 588
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 758 790 3533 3565 758
Size: 32 2743 32 1587
Fragments arranged by size:
2743 1587 32 32
PstI C_TGCA'G
Cuts at: 1441 2126 1441
Size: 685 3709
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 938 1819 2179 938
Size: 881 360 3153
Fragments arranged by size:
3153 881 360
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 1408 1408
Size: 4394
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SfcI C'TryA_G
Cuts at: 33 1113 1437 2122 3115 3306 33
Size: 1080 324 685 993 191 1121
Fragments arranged by size:
1121 1080 993 685 324 191
SfoI GGC'GCC
Cuts at: 398 1826 2074 398
Size: 1428 248 2718
Fragments arranged by size:
2718 1428 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 1416 1416
Size: 4394
SmlI C'TyrA_G
Cuts at: 1093 1549 2464 2956 3218 1093
Size: 456 915 492 262 2269
Fragments arranged by size:
2269 915 492 456 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 203 1447 1947 203
Size: 1244 500 2650
Fragments arranged by size:
2650 1244 500
StuI AGG'CCT
Cuts at: 212 1109 1246 212
Size: 897 137 3360
Fragments arranged by size:
3360 897 137
StyI C'CwwG_G
Cuts at: 107 134 180 191 1528 1882 107
Size: 27 46 11 1337 354 2619
Fragments arranged by size:
2619 1337 354 46 27 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 361 757 1297 1977 2110 2569 2778 361
Size: 396 540 680 133 459 209 1977
Fragments arranged by size:
1977 680 540 459 396 209 133
TatI w'GTAC_w
Cuts at: 215 3909 215
Size: 3694 700
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1213 1245 1342 1374 3911 3943 1213
Size: 32 97 32 2537 32 1664
Fragments arranged by size:
2537 1664 97 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2191 2191
Size: 4394
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1018 1383 1889 1018
Size: 365 506 3523
Fragments arranged by size:
3523 506 365
XmaI C'CCGG_G
Cuts at: 1414 1414
Size: 4394
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I
BsiWI BsmI BssHII BstZ17I ClaI CspCI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI