Restriction map for
pF1KB6673
(Circular) (Six-base) MAPSORT of: pF1KB6673.seq Check: 3789 from: 1 to: 4394
pF1KB6673 4394 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 385 385
Size: 4394
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 563 1410 563
Size: 847 3547
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 486 2219 2651 3398 3666 486
Size: 1733 432 747 268 1214
Fragments arranged by size:
1733 1214 747 432 268
AfeI AGC'GCT
Cuts at: 358 358
Size: 4394
AflIII A'CryG_T
Cuts at: 620 1746 2850 3827 620
Size: 1126 1104 977 1187
Fragments arranged by size:
1187 1126 1104 977
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AlwNI CAG_nnn'CTG
Cuts at: 192 378 1699 3266 192
Size: 186 1321 1567 1320
Fragments arranged by size:
1567 1321 1320 186
ApaI G_GGCC'C
Cuts at: 657 657
Size: 4394
ApaLI G'TGCA_C
Cuts at: 3164 3164
Size: 4394
ApoI r'AATT_y
Cuts at: 1398 3747 4003 1398
Size: 2349 256 1789
Fragments arranged by size:
2349 1789 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 422 464 712 1195 1414 422
Size: 42 248 483 219 3402
Fragments arranged by size:
3402 483 248 219 42
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 429 563 678 1292 1321 1410 1824 2072 2107
Size: 134 115 614 29 89 414 248 35
Cuts at: 2107 429
Size: 2716
Fragments arranged by size:
2716 614 414 248 134 115 89 35
29
BanII G_rGCy'C
Cuts at: 657 671 948 1408 2438 657
Size: 14 277 460 1030 2613
Fragments arranged by size:
2613 1030 460 277 14
BbeI G_GCGC'C
Cuts at: 1325 1828 2076 1325
Size: 503 248 3643
Fragments arranged by size:
3643 503 248
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BglI GCCn_nnn'nGGC
Cuts at: 427 1232 427
Size: 805 3589
BglII A'GATC_T
Cuts at: 1909 3946 1909
Size: 2037 2357
BlpI GC'TnA_GC
Cuts at: 371 1330 1506 371
Size: 959 176 3259
Fragments arranged by size:
3259 959 176
Bme1580I G_kGCm'C
Cuts at: 657 683 2019 2112 3168 657
Size: 26 1336 93 1056 1883
Fragments arranged by size:
1883 1336 1056 93 26
BmrI ACTGGGnnnn_n'
Cuts at: 615 640 1606 1687 2022 4166 615
Size: 25 966 81 335 2144 843
Fragments arranged by size:
2144 966 843 335 81 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 835 1075 631
Size: 204 240 3950
Fragments arranged by size:
3950 240 204
Bpu10I CC'TnA_GC
Cuts at: 1222 1222
Size: 4394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1570 2449 2941 3239 277
Size: 1293 879 492 298 1432
Fragments arranged by size:
1432 1293 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 124 4098 124
Size: 3974 420
BsaAI yAC'GTr
Cuts at: 257 789 951 1284 1747 257
Size: 532 162 333 463 2904
Fragments arranged by size:
2904 532 463 333 162
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 1322 1825 2073 2775 4271 1322
Size: 503 248 702 1496 1445
Fragments arranged by size:
1496 1445 702 503 248
BsaWI w'CCGG_w
Cuts at: 985 1583 2104 2782 3056 3203 3614 985
Size: 598 521 678 274 147 411 1765
Fragments arranged by size:
1765 678 598 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 607 637 808 838 607
Size: 30 171 30 4163
Fragments arranged by size:
4163 171 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 742 1324 742
Size: 582 3812
BseYI C'CCAG_C
Cuts at: 123 641 753 1049 1317 1819 2577 3154 3650
Size: 518 112 296 268 502 758 577 496
Cuts at: 3650 123
Size: 867
Fragments arranged by size:
867 758 577 518 502 496 296 268
112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 631 811 508
Size: 123 180 4091
Fragments arranged by size:
4091 180 123
BsiEI CG_ry'CG
Cuts at: 104 474 1936 1982 3190 104
Size: 370 1462 46 1208 1308
Fragments arranged by size:
1462 1308 1208 370 46
BsiHKAI G_wGCw'C
Cuts at: 224 242 1408 2186 2376 3168 224
Size: 18 1166 778 190 792 1450
Fragments arranged by size:
1450 1166 792 778 190 18
BsmI GAATG_Cn'
Cuts at: 917 917
Size: 4394
Bsp1286I G_dGCh'C
Cuts at: 224 242 657 671 683 948 1408 2019 2112
Size: 18 415 14 12 265 460 611 93
Cuts at: 2112 2186 2376 2438 3168 224
Size: 74 190 62 730 1450
Fragments arranged by size:
1450 730 611 460 415 265 190 93
74 62 18 14 12
BspEI T'CCGG_A
Cuts at: 985 3614 985
Size: 2629 1765
BspHI T'CATG_A
Cuts at: 3570 3570
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 309 385 1444 1658 1960 2341 309
Size: 76 1059 214 302 381 2362
Fragments arranged by size:
2362 1059 381 302 214 76
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 912 2306 912
Size: 1394 3000
BsrFI r'CCGG_y
Cuts at: 635 892 2392 2573 2782 635
Size: 257 1500 181 209 2247
Fragments arranged by size:
2247 1500 257 209 181
BssSI C'ACGA_G
Cuts at: 2665 3023 2665
Size: 358 4036
BstAPI GCAn_nnn'nTGC
Cuts at: 789 1771 789
Size: 982 3412
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstXI CCAn_nnnn'nTGG
Cuts at: 1053 1053
Size: 4394
BstYI r'GATC_y
Cuts at: 1 1419 1886 1909 2244 2490 3502 3588 3600
Size: 1418 467 23 335 246 1012 86 12
Cuts at: 3600 3946 1
Size: 346 449
Fragments arranged by size:
1418 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 521 671 1002 1288 521
Size: 150 331 286 3627
Fragments arranged by size:
3627 331 286 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 1637 1637
Size: 4394
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DraIII CAC_nnn'GTG
Cuts at: 609 1087 609
Size: 478 3916
DrdI GACnn_nn'nnGTC
Cuts at: 2100 2958 2100
Size: 858 3536
EaeI y'GGCC_r
Cuts at: 264 471 524 1078 1979 2153 2544 2571 3916
Size: 207 53 554 901 174 391 27 1345
Cuts at: 3916 264
Size: 742
Fragments arranged by size:
1345 901 742 554 391 207 174 53
27
EagI C'GGCC_G
Cuts at: 471 1979 471
Size: 1508 2886
EarI CTCTTCn'nnn_
Cuts at: 1156 2417 2627 1156
Size: 1261 210 2923
Fragments arranged by size:
2923 1261 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 352 1168 2602 2912 3058 4204 352
Size: 816 1434 310 146 1146 542
Fragments arranged by size:
1434 1146 816 542 310 146
EcoICRI GAG'CTC
Cuts at: 1406 1406
Size: 4394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 486 631 835 1075 2219 2651 3398 3666 486
Size: 145 204 240 1144 432 747 268 1214
Fragments arranged by size:
1214 1144 747 432 268 240 204 145
EcoNI CCTnn'n_nnAGG
Cuts at: 1268 1268
Size: 4394
EcoO109I rG'GnC_Cy
Cuts at: 653 654 993 1131 1261 1533 653
Size: 1 339 138 130 272 3514
Fragments arranged by size:
3514 339 272 138 130 1
EcoRI G'AATT_C
Cuts at: 1398 1398
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 468 2413 468
Size: 1945 2449
FseI GG_CCGG'CC
Cuts at: 639 639
Size: 4394
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 360 1325 1828 2076 3098 4077 360
Size: 965 503 248 1022 979 677
Fragments arranged by size:
1022 979 965 677 503 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 318 350 576 607 608 639 808
Size: 32 184 32 226 31 1 31 169
Cuts at: 808 840 2236 2266 2268 2298 102
Size: 32 1396 30 2 30 2198
Fragments arranged by size:
2198 1396 226 184 169 32 32 32
31 31 30 30 2 1
HincII GTy'rAC
Cuts at: 1433 1433
Size: 4394
KasI G'GCGC_C
Cuts at: 1321 1824 2072 1321
Size: 503 248 3643
Fragments arranged by size:
3643 503 248
KpnI G_GTAC'C
Cuts at: 567 1414 567
Size: 847 3547
MluI A'CGCG_T
Cuts at: 620 3827 620
Size: 3207 1187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3065 3249 3065
Size: 184 4210
MscI TGG'CCA
Cuts at: 526 1080 2155 526
Size: 554 1075 2765
Fragments arranged by size:
2765 1075 554
MslI CAynn'nnrTG
Cuts at: 526 676 2383 2510 526
Size: 150 1707 127 2410
Fragments arranged by size:
2410 1707 150 127
MspA1I CmG'CkG
Cuts at: 1053 1290 1505 1819 2179 3192 3437 1053
Size: 237 215 314 360 1013 245 2010
Fragments arranged by size:
2010 1013 360 314 245 237 215
NaeI GCC'GGC
Cuts at: 637 894 2575 637
Size: 257 1681 2456
Fragments arranged by size:
2456 1681 257
NarI GG'CG_CC
Cuts at: 1322 1825 2073 1322
Size: 503 248 3643
Fragments arranged by size:
3643 503 248
NcoI C'CATG_G
Cuts at: 521 671 521
Size: 150 4244
NgoMIV G'CCGG_C
Cuts at: 635 892 2573 635
Size: 257 1681 2456
Fragments arranged by size:
2456 1681 257
NruI TCG'CGA
Cuts at: 1190 1190
Size: 4394
NspI r_CATG'y
Cuts at: 1447 1947 2854 1447
Size: 500 907 2987
Fragments arranged by size:
2987 907 500
PciI A'CATG_T
Cuts at: 2850 2850
Size: 4394
PflMI CCAn_nnn'nTGG
Cuts at: 885 885
Size: 4394
PfoI T'CCnGG_A
Cuts at: 1022 1022
Size: 4394
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PmlI CAC'GTG
Cuts at: 257 789 951 1284 257
Size: 532 162 333 3367
Fragments arranged by size:
3367 532 333 162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 4362
PpuMI rG'GwC_Cy
Cuts at: 993 1131 1261 993
Size: 138 130 4126
Fragments arranged by size:
4126 138 130
PspOMI G'GGCC_C
Cuts at: 653 653
Size: 4394
PstI C_TGCA'G
Cuts at: 164 311 785 1441 2126 164
Size: 147 474 656 685 2432
Fragments arranged by size:
2432 685 656 474 147
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 1053 1819 2179 1053
Size: 766 360 3268
Fragments arranged by size:
3268 766 360
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 1408 1408
Size: 4394
SacII CC_GC'GG
Cuts at: 1291 1291
Size: 4394
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SanDI GG'GwC_CC
Cuts at: 993 1131 993
Size: 138 4256
SapI GCTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SexAI A'CCwGG_T
Cuts at: 698 698
Size: 4394
SfcI C'TryA_G
Cuts at: 33 160 307 781 1437 2122 3115 3306 33
Size: 127 147 474 656 685 993 191 1121
Fragments arranged by size:
1121 993 685 656 474 191 147 127
SfoI GGC'GCC
Cuts at: 1323 1826 2074 1323
Size: 503 248 3643
Fragments arranged by size:
3643 503 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 424 1416 424
Size: 992 3402
SmlI C'TyrA_G
Cuts at: 292 1549 2464 2956 3218 292
Size: 1257 915 492 262 1468
Fragments arranged by size:
1468 1257 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StuI AGG'CCT
Cuts at: 166 1010 166
Size: 844 3550
StyI C'CwwG_G
Cuts at: 261 521 671 1528 1882 261
Size: 260 150 857 354 2773
Fragments arranged by size:
2773 857 354 260 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 597 1063 1977 2110 2569 2778 597
Size: 466 914 133 459 209 2213
Fragments arranged by size:
2213 914 466 459 209 133
TatI w'GTAC_w
Cuts at: 1169 3909 1169
Size: 2740 1654
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 794 826 3911 3943 794
Size: 32 3085 32 1245
Fragments arranged by size:
3085 1245 32 32
Tth111I GACn'n_nGTC
Cuts at: 2191 2191
Size: 4394
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1889 1889
Size: 4394
XmaI C'CCGG_G
Cuts at: 422 1414 422
Size: 992 3402
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PasI PshAI PsiI PspXI PsrI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI