Restriction map for
pF1KB6671
(Circular) (Six-base) MAPSORT of: pF1KB6671.seq Check: 7676 from: 1 to: 4391
pF1KB6671 4391 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 122 1429 122
Size: 1307 3084
Acc65I G'GTAC_C
Cuts at: 1407 1407
Size: 4391
AclI AA'CG_TT
Cuts at: 4231 4231
Size: 4391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2216 2648 3395 3663 2216
Size: 432 747 268 2944
Fragments arranged by size:
2944 747 432 268
AflIII A'CryG_T
Cuts at: 426 857 1743 2847 3824 426
Size: 431 886 1104 977 993
Fragments arranged by size:
1104 993 977 886 431
AgeI A'CCGG_T
Cuts at: 2779 2779
Size: 4391
AleI CACnn'nnGTG
Cuts at: 226 226
Size: 4391
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1252 1272 1284 1304 1252
Size: 20 12 20 4339
Fragments arranged by size:
4339 20 20 12
AlwNI CAG_nnn'CTG
Cuts at: 1277 1696 3263 1277
Size: 419 1567 2405
Fragments arranged by size:
2405 1567 419
ApaLI G'TGCA_C
Cuts at: 952 3161 952
Size: 2209 2182
ApoI r'AATT_y
Cuts at: 1395 3744 4000 1395
Size: 2349 256 1786
Fragments arranged by size:
2349 1786 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4391
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
AvaI C'yCGr_G
Cuts at: 332 1122 1411 332
Size: 790 289 3312
Fragments arranged by size:
3312 790 289
BamHI G'GATC_C
Cuts at: 1416 1416
Size: 4391
BanI G'GyrC_C
Cuts at: 793 991 1407 1821 2069 2104 793
Size: 198 416 414 248 35 3080
Fragments arranged by size:
3080 416 414 248 198 35
BanII G_rGCy'C
Cuts at: 1405 2435 1405
Size: 1030 3361
BbeI G_GCGC'C
Cuts at: 995 1825 2073 995
Size: 830 248 3313
Fragments arranged by size:
3313 830 248
BbsI GAAGACnn'nnnn_
Cuts at: 1216 1216
Size: 4391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 298 313 332 347 298
Size: 15 19 15 4342
Fragments arranged by size:
4342 19 15 15
BciVI GTATCCnnnnn_n'
Cuts at: 2289 3050 4391 2289
Size: 761 1341 2289
Fragments arranged by size:
2289 1341 761
BclI T'GATC_A
Cuts at: 1911 1911
Size: 4391
BglII A'GATC_T
Cuts at: 296 1906 3943 296
Size: 1610 2037 744
Fragments arranged by size:
2037 1610 744
BlpI GC'TnA_GC
Cuts at: 1503 1503
Size: 4391
Bme1580I G_kGCm'C
Cuts at: 798 956 1312 1384 2016 2109 3165 798
Size: 158 356 72 632 93 1056 2024
Fragments arranged by size:
2024 1056 632 356 158 93 72
BmgBI CAC'GTC
Cuts at: 429 429
Size: 4391
BmrI ACTGGGnnnn_n'
Cuts at: 393 448 1315 1603 1684 2019 4163 393
Size: 55 867 288 81 335 2144 621
Fragments arranged by size:
2144 867 621 335 288 81 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 4391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 289 283
Size: 6 4385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1567 2446 2938 3236 346
Size: 1221 879 492 298 1501
Fragments arranged by size:
1501 1221 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 840 909 4095 840
Size: 69 3186 1136
Fragments arranged by size:
3186 1136 69
BsaAI yAC'GTr
Cuts at: 327 1080 1744 327
Size: 753 664 2974
Fragments arranged by size:
2974 753 664
BsaBI GATnn'nnATC
Cuts at: 6 1327 1905 3942 6
Size: 1321 578 2037 455
Fragments arranged by size:
2037 1321 578 455
BsaHI Gr'CG_yC
Cuts at: 119 992 1822 2070 2772 4268 119
Size: 873 830 248 702 1496 242
Fragments arranged by size:
1496 873 830 702 248 242
BsaWI w'CCGG_w
Cuts at: 1580 2101 2779 3053 3200 3611 1580
Size: 521 678 274 147 411 2360
Fragments arranged by size:
2360 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 122 148 152 178 332 337 362 367 688
Size: 26 4 26 154 5 25 5 321
Cuts at: 688 718 1252 1282 1363 1393 122
Size: 30 534 30 81 30 3120
Fragments arranged by size:
3120 534 321 154 81 30 30 30
26 26 25 5 5 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 262 1363 262
Size: 1101 3290
BseYI C'CCAG_C
Cuts at: 181 190 1816 2574 3151 3647 181
Size: 9 1626 758 577 496 925
Fragments arranged by size:
1626 925 758 577 496 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 433 920 307
Size: 126 487 3778
Fragments arranged by size:
3778 487 126
BsiEI CG_ry'CG
Cuts at: 104 1933 1979 3187 104
Size: 1829 46 1208 1308
Fragments arranged by size:
1829 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 461 569 956 1405 2183 2373 3165 461
Size: 108 387 449 778 190 792 1687
Fragments arranged by size:
1687 792 778 449 387 190 108
Bsp1286I G_dGCh'C
Cuts at: 461 569 798 956 1312 1384 1405 2016 2109
Size: 108 229 158 356 72 21 611 93
Cuts at: 2109 2183 2373 2435 3165 461
Size: 74 190 62 730 1687
Fragments arranged by size:
1687 730 611 356 229 190 158 108
93 74 72 62 21
BspEI T'CCGG_A
Cuts at: 3611 3611
Size: 4391
BspHI T'CATG_A
Cuts at: 3567 3567
Size: 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 455 891 1441 1655 1957 2338 455
Size: 436 550 214 302 381 2508
Fragments arranged by size:
2508 550 436 381 302 214
BsrBI CCG'CTC
Cuts at: 50 113 343 2683 2737 4221 4386 50
Size: 63 230 2340 54 1484 165 55
Fragments arranged by size:
2340 1484 230 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 1238 2303 1238
Size: 1065 3326
BsrFI r'CCGG_y
Cuts at: 1150 2389 2570 2779 1150
Size: 1239 181 209 2762
Fragments arranged by size:
2762 1239 209 181
BssSI C'ACGA_G
Cuts at: 601 2662 3020 601
Size: 2061 358 1972
Fragments arranged by size:
2061 1972 358
BstAPI GCAn_nnn'nTGC
Cuts at: 456 1768 456
Size: 1312 3079
BstBI TT'CG_AA
Cuts at: 2752 2752
Size: 4391
BstEII G'GTnAC_C
Cuts at: 1083 1083
Size: 4391
BstXI CCAn_nnnn'nTGG
Cuts at: 228 228
Size: 4391
BstYI r'GATC_y
Cuts at: 1 296 1328 1416 1883 1906 2241 2487 3499
Size: 295 1032 88 467 23 335 246 1012
Cuts at: 3499 3585 3597 3943 1
Size: 86 12 346 449
Fragments arranged by size:
1032 1012 467 449 346 335 295 246
88 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1013 1013
Size: 4391
BtgI C'CryG_G
Cuts at: 242 475 629 1256 242
Size: 233 154 627 3377
Fragments arranged by size:
3377 627 233 154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2341 2522 2685 2341
Size: 181 163 4047
Fragments arranged by size:
4047 181 163
BtsI GCAGTG_nn'
Cuts at: 718 1162 1369 1634 718
Size: 444 207 265 3475
Fragments arranged by size:
3475 444 265 207
DraI TTT'AAA
Cuts at: 1390 1390
Size: 4391
DraIII CAC_nnn'GTG
Cuts at: 456 1083 456
Size: 627 3764
DrdI GACnn_nn'nnGTC
Cuts at: 2097 2955 2097
Size: 858 3533
EaeI y'GGCC_r
Cuts at: 239 632 737 1976 2150 2541 2568 3913 239
Size: 393 105 1239 174 391 27 1345 717
Fragments arranged by size:
1345 1239 717 393 391 174 105 27
EagI C'GGCC_G
Cuts at: 1976 1976
Size: 4391
EarI CTCTTCn'nnn_
Cuts at: 2414 2624 2414
Size: 210 4181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 143 2599 2909 3055 4201 143
Size: 2456 310 146 1146 333
Fragments arranged by size:
2456 1146 333 310 146
EcoICRI GAG'CTC
Cuts at: 1403 1403
Size: 4391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 289 2216 2648 3395 3663 283
Size: 6 1927 432 747 268 1011
Fragments arranged by size:
1927 1011 747 432 268 6
EcoNI CCTnn'n_nnAGG
Cuts at: 689 689
Size: 4391
EcoO109I rG'GnC_Cy
Cuts at: 1017 1044 1530 1017
Size: 27 486 3878
Fragments arranged by size:
3878 486 27
EcoRI G'AATT_C
Cuts at: 1395 1395
Size: 4391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 231 2410 231
Size: 2179 2212
FspI TGC'GCA
Cuts at: 2172 2172
Size: 4391
HaeII r_GCGC'y
Cuts at: 148 995 1825 2073 3095 4074 148
Size: 847 830 248 1022 979 465
Fragments arranged by size:
1022 979 847 830 465 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 148 180 332 337 364 369 688 720 992
Size: 32 152 5 27 5 319 32 272
Cuts at: 992 1024 1220 1252 2233 2263 2265 2295 148
Size: 32 196 32 981 30 2 30 2244
Fragments arranged by size:
2244 981 319 272 196 152 32 32
32 32 30 30 27 5 5 2
HincII GTy'rAC
Cuts at: 1430 1430
Size: 4391
KasI G'GCGC_C
Cuts at: 991 1821 2069 991
Size: 830 248 3313
Fragments arranged by size:
3313 830 248
KpnI G_GTAC'C
Cuts at: 1411 1411
Size: 4391
MluI A'CGCG_T
Cuts at: 3824 3824
Size: 4391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3062 3246 3062
Size: 184 4207
MscI TGG'CCA
Cuts at: 739 2152 739
Size: 1413 2978
MslI CAynn'nnrTG
Cuts at: 226 706 1099 2380 2507 226
Size: 480 393 1281 127 2110
Fragments arranged by size:
2110 1281 480 393 127
MspA1I CmG'CkG
Cuts at: 244 1502 1816 2176 3189 3434 244
Size: 1258 314 360 1013 245 1201
Fragments arranged by size:
1258 1201 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2572 2572
Size: 4391
NarI GG'CG_CC
Cuts at: 992 1822 2070 992
Size: 830 248 3313
Fragments arranged by size:
3313 830 248
NcoI C'CATG_G
Cuts at: 1256 1256
Size: 4391
NgoMIV G'CCGG_C
Cuts at: 2570 2570
Size: 4391
NspI r_CATG'y
Cuts at: 861 1104 1227 1444 1944 2851 861
Size: 243 123 217 500 907 2401
Fragments arranged by size:
2401 907 500 243 217 123
PciI A'CATG_T
Cuts at: 857 2847 857
Size: 1990 2401
PflMI CCAn_nnn'nTGG
Cuts at: 735 845 735
Size: 110 4281
PfoI T'CCnGG_A
Cuts at: 404 728 785 875 404
Size: 324 57 90 3920
Fragments arranged by size:
3920 324 90 57
PmeI GTTT'AAAC
Cuts at: 1390 1390
Size: 4391
PmlI CAC'GTG
Cuts at: 327 1080 327
Size: 753 3638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 502 534 1252 1284 3530 3562 502
Size: 32 718 32 2246 32 1331
Fragments arranged by size:
2246 1331 718 32 32 32
PstI C_TGCA'G
Cuts at: 665 902 1172 1438 2123 665
Size: 237 270 266 685 2933
Fragments arranged by size:
2933 685 270 266 237
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4391
PvuII CAG'CTG
Cuts at: 1816 2176 1816
Size: 360 4031
RsrII CG'GwC_CG
Cuts at: 2586 2586
Size: 4391
SacI G_AGCT'C
Cuts at: 1405 1405
Size: 4391
SacII CC_GC'GG
Cuts at: 245 245
Size: 4391
SalI G'TCGA_C
Cuts at: 1428 1428
Size: 4391
SapI GCTCTTCn'nnn_
Cuts at: 2414 2624 2414
Size: 210 4181
SbfI CC_TGCA'GG
Cuts at: 1438 1438
Size: 4391
ScaI AGT'ACT
Cuts at: 3908 3908
Size: 4391
SfcI C'TryA_G
Cuts at: 33 417 661 898 1168 1434 2119 3112 3303
Size: 384 244 237 270 266 685 993 191
Cuts at: 3303 33
Size: 1121
Fragments arranged by size:
1121 993 685 384 270 266 244 237
191
SfoI GGC'GCC
Cuts at: 993 1823 2071 993
Size: 830 248 3313
Fragments arranged by size:
3313 830 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
SgrAI Cr'CCGG_yG
Cuts at: 1150 1150
Size: 4391
SmaI CCC'GGG
Cuts at: 1124 1413 1124
Size: 289 4102
SmlI C'TyrA_G
Cuts at: 361 1546 2461 2953 3215 361
Size: 1185 915 492 262 1537
Fragments arranged by size:
1537 1185 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4391
SphI G_CATG'C
Cuts at: 1227 1444 1944 1227
Size: 217 500 3674
Fragments arranged by size:
3674 500 217
SrfI GCCC'GGGC
Cuts at: 1124 1124
Size: 4391
StuI AGG'CCT
Cuts at: 889 889
Size: 4391
StyI C'CwwG_G
Cuts at: 206 773 1256 1525 1879 206
Size: 567 483 269 354 2718
Fragments arranged by size:
2718 567 483 354 269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 467 637 1057 1974 2107 2566 2775 467
Size: 170 420 917 133 459 209 2083
Fragments arranged by size:
2083 917 459 420 209 170 133
TatI w'GTAC_w
Cuts at: 527 642 3906 527
Size: 115 3264 1012
Fragments arranged by size:
3264 1012 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 122 154 373 405 621 653 1363 1395 3908
Size: 32 219 32 216 32 710 32 2513
Cuts at: 3908 3940 122
Size: 32 573
Fragments arranged by size:
2513 710 573 219 216 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2188 2188
Size: 4391
XbaI T'CTAG_A
Cuts at: 1422 1422
Size: 4391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1068 1886 1068
Size: 818 3573
XmaI C'CCGG_G
Cuts at: 1122 1411 1122
Size: 289 4102
XmnI GAAnn'nnTTC
Cuts at: 298 298
Size: 4391
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PmlI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SnaBI SspI SwaI
XhoI ZraI