Restriction map for
pF1KB6659
(Circular) (Six-base) MAPSORT of: pF1KB6659.seq Check: 8809 from: 1 to: 4385
pF1KB6659 4385 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 1423 1423
Size: 4385
Acc65I G'GTAC_C
Cuts at: 1401 1401
Size: 4385
AclI AA'CG_TT
Cuts at: 4225 4225
Size: 4385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 555 598 956 1373 2210 2642 3389 3657 555
Size: 43 358 417 837 432 747 268 1283
Fragments arranged by size:
1283 837 747 432 417 358 268 43
AflIII A'CryG_T
Cuts at: 1737 2841 3818 1737
Size: 1104 977 2304
Fragments arranged by size:
2304 1104 977
AgeI A'CCGG_T
Cuts at: 2773 2773
Size: 4385
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 941 973 941
Size: 32 4353
AlwNI CAG_nnn'CTG
Cuts at: 1098 1690 3257 1098
Size: 592 1567 2226
Fragments arranged by size:
2226 1567 592
ApaLI G'TGCA_C
Cuts at: 3155 3155
Size: 4385
ApoI r'AATT_y
Cuts at: 1389 3738 3994 1389
Size: 2349 256 1780
Fragments arranged by size:
2349 1780 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4385
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
AvaI C'yCGr_G
Cuts at: 193 438 1405 193
Size: 245 967 3173
Fragments arranged by size:
3173 967 245
BamHI G'GATC_C
Cuts at: 1410 1410
Size: 4385
BanI G'GyrC_C
Cuts at: 1401 1815 2063 2098 1401
Size: 414 248 35 3688
Fragments arranged by size:
3688 414 248 35
BanII G_rGCy'C
Cuts at: 148 1108 1399 2429 148
Size: 960 291 1030 2104
Fragments arranged by size:
2104 1030 960 291
BbeI G_GCGC'C
Cuts at: 1819 2067 1819
Size: 248 4137
BbsI GAAGACnn'nnnn_
Cuts at: 1244 1244
Size: 4385
BbvCI CC'TCA_GC
Cuts at: 563 563
Size: 4385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 442 476 505 539 442
Size: 34 29 34 4288
Fragments arranged by size:
4288 34 34 29
BciVI GTATCCnnnnn_n'
Cuts at: 2283 3044 4385 2283
Size: 761 1341 2283
Fragments arranged by size:
2283 1341 761
BclI T'GATC_A
Cuts at: 1905 1905
Size: 4385
BglI GCCn_nnn'nGGC
Cuts at: 442 442
Size: 4385
BglII A'GATC_T
Cuts at: 1900 3937 1900
Size: 2037 2348
BlpI GC'TnA_GC
Cuts at: 1497 1497
Size: 4385
Bme1580I G_kGCm'C
Cuts at: 2010 2103 3159 2010
Size: 93 1056 3236
Fragments arranged by size:
3236 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1597 1678 2013 4157 1597
Size: 81 335 2144 1825
Fragments arranged by size:
2144 1825 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 4385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 245 400 782 991 1075 1132 237
Size: 8 155 382 209 84 57 3490
Fragments arranged by size:
3490 382 209 155 84 57 8
Bpu10I CC'TnA_GC
Cuts at: 142 563 142
Size: 421 3964
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1561 2440 2932 3230 1015
Size: 546 879 492 298 2170
Fragments arranged by size:
2170 879 546 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1048 4089 1048
Size: 3041 1344
BsaAI yAC'GTr
Cuts at: 1738 1738
Size: 4385
BsaBI GATnn'nnATC
Cuts at: 6 1899 3936 6
Size: 1893 2037 455
Fragments arranged by size:
2037 1893 455
BsaHI Gr'CG_yC
Cuts at: 815 1816 2064 2766 4262 815
Size: 1001 248 702 1496 938
Fragments arranged by size:
1496 1001 938 702 248
BsaWI w'CCGG_w
Cuts at: 1574 2095 2773 3047 3194 3605 1574
Size: 521 678 274 147 411 2354
Fragments arranged by size:
2354 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1226 1256 1226
Size: 30 4355
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 138 706 1066 138
Size: 568 360 3457
Fragments arranged by size:
3457 568 360
BseYI C'CCAG_C
Cuts at: 205 1810 2568 3145 3641 205
Size: 1605 758 577 496 949
Fragments arranged by size:
1605 949 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1927 1973 3181 104
Size: 1823 46 1208 1308
Fragments arranged by size:
1823 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 148 1043 1108 1399 2177 2367 3159 148
Size: 895 65 291 778 190 792 1374
Fragments arranged by size:
1374 895 792 778 291 190 65
BsmBI CGTCTCn'nnnn_
Cuts at: 969 969
Size: 4385
Bsp1286I G_dGCh'C
Cuts at: 148 1043 1108 1399 2010 2103 2177 2367 2429
Size: 895 65 291 611 93 74 190 62
Cuts at: 2429 3159 148
Size: 730 1374
Fragments arranged by size:
1374 895 730 611 291 190 93 74
65 62
BspEI T'CCGG_A
Cuts at: 3605 3605
Size: 4385
BspHI T'CATG_A
Cuts at: 821 3561 821
Size: 2740 1645
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1435 1649 1951 2332 1435
Size: 214 302 381 3488
Fragments arranged by size:
3488 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2677 2731 4215 4380 50
Size: 2627 54 1484 165 55
Fragments arranged by size:
2627 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 318 2297 318
Size: 1979 2406
BsrFI r'CCGG_y
Cuts at: 2383 2564 2773 2383
Size: 181 209 3995
Fragments arranged by size:
3995 209 181
BssSI C'ACGA_G
Cuts at: 2656 3014 2656
Size: 358 4027
BstAPI GCAn_nnn'nTGC
Cuts at: 1762 1762
Size: 4385
BstBI TT'CG_AA
Cuts at: 2746 2746
Size: 4385
BstYI r'GATC_y
Cuts at: 1 1410 1877 1900 2235 2481 3493 3579 3591
Size: 1409 467 23 335 246 1012 86 12
Cuts at: 3591 3937 1
Size: 346 449
Fragments arranged by size:
1409 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4385
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2335 2516 2679 2335
Size: 181 163 4041
Fragments arranged by size:
4041 181 163
BtsI GCAGTG_nn'
Cuts at: 424 1628 424
Size: 1204 3181
DraI TTT'AAA
Cuts at: 1384 1384
Size: 4385
DraIII CAC_nnn'GTG
Cuts at: 264 264
Size: 4385
DrdI GACnn_nn'nnGTC
Cuts at: 2091 2949 2091
Size: 858 3527
EaeI y'GGCC_r
Cuts at: 323 1970 2144 2535 2562 3907 323
Size: 1647 174 391 27 1345 801
Fragments arranged by size:
1647 1345 801 391 174 27
EagI C'GGCC_G
Cuts at: 1970 1970
Size: 4385
EarI CTCTTCn'nnn_
Cuts at: 476 1271 2408 2618 476
Size: 795 1137 210 2243
Fragments arranged by size:
2243 1137 795 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2593 2903 3049 4195 2593
Size: 310 146 1146 2783
Fragments arranged by size:
2783 1146 310 146
EcoICRI GAG'CTC
Cuts at: 146 1106 1397 146
Size: 960 291 3134
Fragments arranged by size:
3134 960 291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 245 400 555 598 782 956 991 1075
Size: 8 155 155 43 184 174 35 84
Cuts at: 1075 1132 1373 2210 2642 3389 3657 237
Size: 57 241 837 432 747 268 965
Fragments arranged by size:
965 837 747 432 268 241 184 174
155 155 84 57 43 35 8
EcoNI CCTnn'n_nnAGG
Cuts at: 364 637 721 871 364
Size: 273 84 150 3878
Fragments arranged by size:
3878 273 150 84
EcoO109I rG'GnC_Cy
Cuts at: 159 297 1524 159
Size: 138 1227 3020
Fragments arranged by size:
3020 1227 138
EcoRI G'AATT_C
Cuts at: 1389 1389
Size: 4385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4385
FspI TGC'GCA
Cuts at: 2166 2166
Size: 4385
HaeII r_GCGC'y
Cuts at: 192 1819 2067 3089 4068 192
Size: 1627 248 1022 979 509
Fragments arranged by size:
1627 1022 979 509 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 432 464 2227 2257 2259 2289 432
Size: 32 1763 30 2 30 2528
Fragments arranged by size:
2528 1763 32 30 30 2
HincII GTy'rAC
Cuts at: 1424 1424
Size: 4385
HindIII A'AGCT_T
Cuts at: 126 126
Size: 4385
KasI G'GCGC_C
Cuts at: 1815 2063 1815
Size: 248 4137
KpnI G_GTAC'C
Cuts at: 1405 1405
Size: 4385
MluI A'CGCG_T
Cuts at: 3818 3818
Size: 4385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 272 413 3056 3240 272
Size: 141 2643 184 1417
Fragments arranged by size:
2643 1417 184 141
MscI TGG'CCA
Cuts at: 325 2146 325
Size: 1821 2564
MslI CAynn'nnrTG
Cuts at: 2374 2501 2374
Size: 127 4258
MspA1I CmG'CkG
Cuts at: 1496 1810 2170 3183 3428 1496
Size: 314 360 1013 245 2453
Fragments arranged by size:
2453 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 4385
NarI GG'CG_CC
Cuts at: 1816 2064 1816
Size: 248 4137
NcoI C'CATG_G
Cuts at: 107 107
Size: 4385
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 4385
NruI TCG'CGA
Cuts at: 452 452
Size: 4385
NspI r_CATG'y
Cuts at: 186 1438 1938 2845 186
Size: 1252 500 907 1726
Fragments arranged by size:
1726 1252 907 500
PciI A'CATG_T
Cuts at: 2841 2841
Size: 4385
PmeI GTTT'AAAC
Cuts at: 1384 1384
Size: 4385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 4353
PpuMI rG'GwC_Cy
Cuts at: 297 297
Size: 4385
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1147 1179 1147
Size: 32 4353
PstI C_TGCA'G
Cuts at: 906 914 1432 2117 906
Size: 8 518 685 3174
Fragments arranged by size:
3174 685 518 8
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4385
PvuII CAG'CTG
Cuts at: 1810 2170 1810
Size: 360 4025
RsrII CG'GwC_CG
Cuts at: 2580 2580
Size: 4385
SacI G_AGCT'C
Cuts at: 148 1108 1399 148
Size: 960 291 3134
Fragments arranged by size:
3134 960 291
SalI G'TCGA_C
Cuts at: 1422 1422
Size: 4385
SapI GCTCTTCn'nnn_
Cuts at: 476 2408 2618 476
Size: 1932 210 2243
Fragments arranged by size:
2243 1932 210
SbfI CC_TGCA'GG
Cuts at: 1432 1432
Size: 4385
ScaI AGT'ACT
Cuts at: 3902 3902
Size: 4385
SfcI C'TryA_G
Cuts at: 33 164 456 902 910 1250 1428 2113 3106
Size: 131 292 446 8 340 178 685 993
Cuts at: 3106 3297 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 446 340 292 191 178
131 8
SfoI GGC'GCC
Cuts at: 1817 2065 1817
Size: 248 4137
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
SmaI CCC'GGG
Cuts at: 1407 1407
Size: 4385
SmlI C'TyrA_G
Cuts at: 994 1540 2455 2947 3209 994
Size: 546 915 492 262 2170
Fragments arranged by size:
2170 915 546 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4385
SphI G_CATG'C
Cuts at: 186 1438 1938 186
Size: 1252 500 2633
Fragments arranged by size:
2633 1252 500
StuI AGG'CCT
Cuts at: 632 803 632
Size: 171 4214
StyI C'CwwG_G
Cuts at: 107 627 810 1058 1519 1873 107
Size: 520 183 248 461 354 2619
Fragments arranged by size:
2619 520 461 354 248 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 219 1968 2101 2560 2769 219
Size: 1749 133 459 209 1835
Fragments arranged by size:
1835 1749 459 209 133
TatI w'GTAC_w
Cuts at: 172 3900 172
Size: 3728 657
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3902 3934 3902
Size: 32 4353
Tth111I GACn'n_nGTC
Cuts at: 2182 2182
Size: 4385
XbaI T'CTAG_A
Cuts at: 1047 1416 1047
Size: 369 4016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1880 1880
Size: 4385
XmaI C'CCGG_G
Cuts at: 1405 1405
Size: 4385
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PciI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BfrBI BmgBI BmtI BsgI BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI