Restriction map for
pF1KB6654
(Circular) (Six-base) MAPSORT of: pF1KB6654.seq Check: 6866 from: 1 to: 4280
pF1KB6654 4280 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 191 1318 191
Size: 1127 3153
Acc65I G'GTAC_C
Cuts at: 1296 1296
Size: 4280
AclI AA'CG_TT
Cuts at: 4120 4120
Size: 4280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 2105 2537 3284 3552 193
Size: 1912 432 747 268 921
Fragments arranged by size:
1912 921 747 432 268
AflIII A'CryG_T
Cuts at: 777 1632 2736 3713 777
Size: 855 1104 977 1344
Fragments arranged by size:
1344 1104 977 855
AgeI A'CCGG_T
Cuts at: 2668 2668
Size: 4280
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 998 1030 998
Size: 32 4248
AlwNI CAG_nnn'CTG
Cuts at: 156 1585 3152 156
Size: 1429 1567 1284
Fragments arranged by size:
1567 1429 1284
ApaLI G'TGCA_C
Cuts at: 3050 3050
Size: 4280
ApoI r'AATT_y
Cuts at: 679 1248 1284 3633 3889 679
Size: 569 36 2349 256 1070
Fragments arranged by size:
2349 1070 569 256 36
AseI AT'TA_AT
Cuts at: 20 20
Size: 4280
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
AvaI C'yCGr_G
Cuts at: 469 1300 469
Size: 831 3449
BamHI G'GATC_C
Cuts at: 1305 1305
Size: 4280
BanI G'GyrC_C
Cuts at: 1296 1710 1958 1993 1296
Size: 414 248 35 3583
Fragments arranged by size:
3583 414 248 35
BanII G_rGCy'C
Cuts at: 182 272 1294 2324 182
Size: 90 1022 1030 2138
Fragments arranged by size:
2138 1030 1022 90
BbeI G_GCGC'C
Cuts at: 1714 1962 1714
Size: 248 4032
BbsI GAAGACnn'nnnn_
Cuts at: 631 631
Size: 4280
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4246
BciVI GTATCCnnnnn_n'
Cuts at: 2178 2939 4280 2178
Size: 761 1341 2178
Fragments arranged by size:
2178 1341 761
BclI T'GATC_A
Cuts at: 1800 1800
Size: 4280
BglII A'GATC_T
Cuts at: 1795 3832 1795
Size: 2037 2243
BlpI GC'TnA_GC
Cuts at: 181 1392 181
Size: 1211 3069
Bme1580I G_kGCm'C
Cuts at: 1905 1998 3054 1905
Size: 93 1056 3131
Fragments arranged by size:
3131 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 462 741 1120 1492 1573 1908 4052 462
Size: 279 379 372 81 335 2144 690
Fragments arranged by size:
2144 690 379 372 335 279 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 4280
Bpu10I CC'TnA_GC
Cuts at: 217 217
Size: 4280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1456 2335 2827 3125 829
Size: 627 879 492 298 1984
Fragments arranged by size:
1984 879 627 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3984 3984
Size: 4280
BsaAI yAC'GTr
Cuts at: 1633 1633
Size: 4280
BsaBI GATnn'nnATC
Cuts at: 6 1794 3831 6
Size: 1788 2037 455
Fragments arranged by size:
2037 1788 455
BsaHI Gr'CG_yC
Cuts at: 1711 1959 2661 4157 1711
Size: 248 702 1496 1834
Fragments arranged by size:
1834 1496 702 248
BsaWI w'CCGG_w
Cuts at: 817 1469 1990 2668 2942 3089 3500 817
Size: 652 521 678 274 147 411 1597
Fragments arranged by size:
1597 678 652 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 267 436 487 979 267
Size: 169 51 492 3568
Fragments arranged by size:
3568 492 169 51
BseYI C'CCAG_C
Cuts at: 1705 2463 3040 3536 1705
Size: 758 577 496 2449
Fragments arranged by size:
2449 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1822 1868 3076 104
Size: 1718 46 1208 1308
Fragments arranged by size:
1718 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1294 2072 2262 3054 1294
Size: 778 190 792 2520
Fragments arranged by size:
2520 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 182 272 1294 1905 1998 2072 2262 2324 3054
Size: 90 1022 611 93 74 190 62 730
Cuts at: 3054 182
Size: 1408
Fragments arranged by size:
1408 1022 730 611 190 93 90 74
62
BspEI T'CCGG_A
Cuts at: 3500 3500
Size: 4280
BspHI T'CATG_A
Cuts at: 3456 3456
Size: 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1330 1544 1846 2227 1330
Size: 214 302 381 3383
Fragments arranged by size:
3383 381 302 214
BsrBI CCG'CTC
Cuts at: 50 129 221 2572 2626 4110 4275 50
Size: 79 92 2351 54 1484 165 55
Fragments arranged by size:
2351 1484 165 92 79 55 54
BsrDI GCAATG_nn'
Cuts at: 2192 2192
Size: 4280
BsrFI r'CCGG_y
Cuts at: 210 2278 2459 2668 210
Size: 2068 181 209 1822
Fragments arranged by size:
2068 1822 209 181
BssSI C'ACGA_G
Cuts at: 2551 2909 2551
Size: 358 3922
BstAPI GCAn_nnn'nTGC
Cuts at: 156 1657 156
Size: 1501 2779
BstBI TT'CG_AA
Cuts at: 2641 2641
Size: 4280
BstYI r'GATC_y
Cuts at: 1 257 663 1305 1772 1795 2130 2376 3388
Size: 256 406 642 467 23 335 246 1012
Cuts at: 3388 3474 3486 3832 1
Size: 86 12 346 449
Fragments arranged by size:
1012 642 467 449 406 346 335 256
246 86 23 12
BtgI C'CryG_G
Cuts at: 272 608 272
Size: 336 3944
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 533 2230 2411 2574 533
Size: 1697 181 163 2239
Fragments arranged by size:
2239 1697 181 163
BtsI GCAGTG_nn'
Cuts at: 803 1523 803
Size: 720 3560
DraI TTT'AAA
Cuts at: 1279 1279
Size: 4280
DrdI GACnn_nn'nnGTC
Cuts at: 1986 2844 1986
Size: 858 3422
EaeI y'GGCC_r
Cuts at: 236 1865 2039 2430 2457 3802 236
Size: 1629 174 391 27 1345 714
Fragments arranged by size:
1629 1345 714 391 174 27
EagI C'GGCC_G
Cuts at: 1865 1865
Size: 4280
EarI CTCTTCn'nnn_
Cuts at: 691 1192 2303 2513 691
Size: 501 1111 210 2458
Fragments arranged by size:
2458 1111 501 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2488 2798 2944 4090 2488
Size: 310 146 1146 2678
Fragments arranged by size:
2678 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1292 1292
Size: 4280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 2105 2537 3284 3552 193
Size: 1912 432 747 268 921
Fragments arranged by size:
1912 921 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 436 1021 436
Size: 585 3695
EcoO109I rG'GnC_Cy
Cuts at: 224 1419 224
Size: 1195 3085
EcoRI G'AATT_C
Cuts at: 1248 1284 1248
Size: 36 4244
EcoRV GAT'ATC
Cuts at: 375 375
Size: 4280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1203 2299 1203
Size: 1096 3184
FspI TGC'GCA
Cuts at: 2061 2061
Size: 4280
HaeII r_GCGC'y
Cuts at: 1714 1962 2984 3963 1714
Size: 248 1022 979 2031
Fragments arranged by size:
2031 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1221 1253 2122 2152 2154 2184 1221
Size: 32 869 30 2 30 3317
Fragments arranged by size:
3317 869 32 30 30 2
HincII GTy'rAC
Cuts at: 192 1319 192
Size: 1127 3153
KasI G'GCGC_C
Cuts at: 1710 1958 1710
Size: 248 4032
KpnI G_GTAC'C
Cuts at: 1300 1300
Size: 4280
MluI A'CGCG_T
Cuts at: 3713 3713
Size: 4280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1264 2951 3135 1264
Size: 1687 184 2409
Fragments arranged by size:
2409 1687 184
MscI TGG'CCA
Cuts at: 238 2041 238
Size: 1803 2477
MslI CAynn'nnrTG
Cuts at: 1048 1063 2269 2396 1048
Size: 15 1206 127 2932
Fragments arranged by size:
2932 1206 127 15
MspA1I CmG'CkG
Cuts at: 156 171 295 321 584 1391 1705 2065 3078
Size: 15 124 26 263 807 314 360 1013
Cuts at: 3078 3323 156
Size: 245 1113
Fragments arranged by size:
1113 1013 807 360 314 263 245 124
26 15
NaeI GCC'GGC
Cuts at: 212 2461 212
Size: 2249 2031
NarI GG'CG_CC
Cuts at: 1711 1959 1711
Size: 248 4032
NcoI C'CATG_G
Cuts at: 608 608
Size: 4280
NgoMIV G'CCGG_C
Cuts at: 210 2459 210
Size: 2249 2031
NspI r_CATG'y
Cuts at: 1333 1833 2740 1333
Size: 500 907 2873
Fragments arranged by size:
2873 907 500
PciI A'CATG_T
Cuts at: 2736 2736
Size: 4280
PflMI CCAn_nnn'nTGG
Cuts at: 498 498
Size: 4280
PfoI T'CCnGG_A
Cuts at: 263 263
Size: 4280
PmeI GTTT'AAAC
Cuts at: 1279 1279
Size: 4280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3419 3451 3419
Size: 32 4248
PpuMI rG'GwC_Cy
Cuts at: 224 224
Size: 4280
PshAI GACnn'nnGTC
Cuts at: 229 229
Size: 4280
PstI C_TGCA'G
Cuts at: 161 798 1327 2012 161
Size: 637 529 685 2429
Fragments arranged by size:
2429 685 637 529
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4280
PvuII CAG'CTG
Cuts at: 156 295 1705 2065 156
Size: 139 1410 360 2371
Fragments arranged by size:
2371 1410 360 139
RsrII CG'GwC_CG
Cuts at: 2475 2475
Size: 4280
SacI G_AGCT'C
Cuts at: 1294 1294
Size: 4280
SalI G'TCGA_C
Cuts at: 190 1317 190
Size: 1127 3153
SanDI GG'GwC_CC
Cuts at: 224 224
Size: 4280
SapI GCTCTTCn'nnn_
Cuts at: 2303 2513 2303
Size: 210 4070
SbfI CC_TGCA'GG
Cuts at: 1327 1327
Size: 4280
ScaI AGT'ACT
Cuts at: 3797 3797
Size: 4280
SexAI A'CCwGG_T
Cuts at: 944 944
Size: 4280
SfcI C'TryA_G
Cuts at: 33 157 794 1323 2008 3001 3192 33
Size: 124 637 529 685 993 191 1121
Fragments arranged by size:
1121 993 685 637 529 191 124
SfoI GGC'GCC
Cuts at: 1712 1960 1712
Size: 248 4032
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
SmaI CCC'GGG
Cuts at: 1302 1302
Size: 4280
SmlI C'TyrA_G
Cuts at: 844 1435 2350 2842 3104 844
Size: 591 915 492 262 2020
Fragments arranged by size:
2020 915 591 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4280
SphI G_CATG'C
Cuts at: 1333 1833 1333
Size: 500 3780
SspI AAT'ATT
Cuts at: 1164 1164
Size: 4280
StuI AGG'CCT
Cuts at: 1027 1027
Size: 4280
StyI C'CwwG_G
Cuts at: 437 608 1259 1414 1768 437
Size: 171 651 155 354 2949
Fragments arranged by size:
2949 651 354 171 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1863 1996 2455 2664 1863
Size: 133 459 209 3479
Fragments arranged by size:
3479 459 209 133
TatI w'GTAC_w
Cuts at: 3795 3795
Size: 4280
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 442 474 3797 3829 442
Size: 32 3323 32 893
Fragments arranged by size:
3323 893 32 32
Tth111I GACn'n_nGTC
Cuts at: 2077 2077
Size: 4280
XbaI T'CTAG_A
Cuts at: 1311 1311
Size: 4280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1775 1775
Size: 4280
XmaI C'CCGG_G
Cuts at: 1300 1300
Size: 4280
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BpmI BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PsiI PspOMI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI
ZraI