Restriction map for
pF1KB6653
(Circular) (Six-base) MAPSORT of: pF1KB6653.seq Check: 8031 from: 1 to: 4340
pF1KB6653 4340 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 292 292
Size: 4340
AccI GT'mk_AC
Cuts at: 536 1378 536
Size: 842 3498
Acc65I G'GTAC_C
Cuts at: 1356 1356
Size: 4340
AclI AA'CG_TT
Cuts at: 4180 4180
Size: 4340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 641 825 1315 2165 2597 3344 3612 274
Size: 367 184 490 850 432 747 268 1002
Fragments arranged by size:
1002 850 747 490 432 367 268 184
AflIII A'CryG_T
Cuts at: 1692 2796 3773 1692
Size: 1104 977 2259
Fragments arranged by size:
2259 1104 977
AgeI A'CCGG_T
Cuts at: 692 2728 692
Size: 2036 2304
AleI CACnn'nnGTG
Cuts at: 1024 1024
Size: 4340
AlwNI CAG_nnn'CTG
Cuts at: 1645 3212 1645
Size: 1567 2773
ApaLI G'TGCA_C
Cuts at: 3110 3110
Size: 4340
ApoI r'AATT_y
Cuts at: 1231 1344 3693 3949 1231
Size: 113 2349 256 1622
Fragments arranged by size:
2349 1622 256 113
AseI AT'TA_AT
Cuts at: 20 20
Size: 4340
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4340
AvaI C'yCGr_G
Cuts at: 121 706 1100 1360 121
Size: 585 394 260 3101
Fragments arranged by size:
3101 585 394 260
BamHI G'GATC_C
Cuts at: 1365 1365
Size: 4340
BanI G'GyrC_C
Cuts at: 378 1036 1356 1770 2018 2053 378
Size: 658 320 414 248 35 2665
Fragments arranged by size:
2665 658 414 320 248 35
BanII G_rGCy'C
Cuts at: 151 749 1354 2384 151
Size: 598 605 1030 2107
Fragments arranged by size:
2107 1030 605 598
BbeI G_GCGC'C
Cuts at: 1774 2022 1774
Size: 248 4092
BbsI GAAGACnn'nnnn_
Cuts at: 126 314 126
Size: 188 4152
BbvCI CC'TCA_GC
Cuts at: 424 424
Size: 4340
BciVI GTATCCnnnnn_n'
Cuts at: 507 2238 2999 4340 507
Size: 1731 761 1341 507
Fragments arranged by size:
1731 1341 761 507
BclI T'GATC_A
Cuts at: 1323 1860 1323
Size: 537 3803
BglII A'GATC_T
Cuts at: 1855 3892 1855
Size: 2037 2303
BlpI GC'TnA_GC
Cuts at: 943 1452 943
Size: 509 3831
Bme1580I G_kGCm'C
Cuts at: 383 1965 2058 3114 383
Size: 1582 93 1056 1609
Fragments arranged by size:
1609 1582 1056 93
BmgBI CAC'GTC
Cuts at: 768 768
Size: 4340
BmrI ACTGGGnnnn_n'
Cuts at: 846 1176 1552 1633 1968 4112 846
Size: 330 376 81 335 2144 1074
Fragments arranged by size:
2144 1074 376 335 330 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2707 2707
Size: 4340
Bpu10I CC'TnA_GC
Cuts at: 424 424
Size: 4340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 1516 2395 2887 3185 973
Size: 543 879 492 298 2128
Fragments arranged by size:
2128 879 543 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 973 4044 973
Size: 3071 1269
BsaAI yAC'GTr
Cuts at: 1693 1693
Size: 4340
BsaBI GATnn'nnATC
Cuts at: 6 1854 3891 6
Size: 1848 2037 455
Fragments arranged by size:
2037 1848 455
BsaHI Gr'CG_yC
Cuts at: 1771 2019 2721 4217 1771
Size: 248 702 1496 1894
Fragments arranged by size:
1894 1496 702 248
BsaWI w'CCGG_w
Cuts at: 692 1194 1529 2050 2728 3002 3149 3560 692
Size: 502 335 521 678 274 147 411 1472
Fragments arranged by size:
1472 678 521 502 411 335 274 147
BseYI C'CCAG_C
Cuts at: 321 432 650 901 1053 1765 2523 3100 3596
Size: 111 218 251 152 712 758 577 496
Cuts at: 3596 321
Size: 1065
Fragments arranged by size:
1065 758 712 577 496 251 218 152
111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 685 643
Size: 42 4298
BsiEI CG_ry'CG
Cuts at: 104 1882 1928 3136 104
Size: 1778 46 1208 1308
Fragments arranged by size:
1778 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1354 2132 2322 3114 1354
Size: 778 190 792 2580
Fragments arranged by size:
2580 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 151 383 749 1354 1965 2058 2132 2322 2384
Size: 232 366 605 611 93 74 190 62
Cuts at: 2384 3114 151
Size: 730 1377
Fragments arranged by size:
1377 730 611 605 366 232 190 93
74 62
BspEI T'CCGG_A
Cuts at: 1194 3560 1194
Size: 2366 1974
BspHI T'CATG_A
Cuts at: 3516 3516
Size: 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 292 1390 1604 1906 2287 292
Size: 1098 214 302 381 2345
Fragments arranged by size:
2345 1098 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1192 2632 2686 4170 4335 50
Size: 1142 1440 54 1484 165 55
Fragments arranged by size:
1484 1440 1142 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2252 2252
Size: 4340
BsrFI r'CCGG_y
Cuts at: 416 692 2338 2519 2728 416
Size: 276 1646 181 209 2028
Fragments arranged by size:
2028 1646 276 209 181
BsrGI T'GTAC_A
Cuts at: 923 923
Size: 4340
BssSI C'ACGA_G
Cuts at: 994 2611 2969 994
Size: 1617 358 2365
Fragments arranged by size:
2365 1617 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1717 1717
Size: 4340
BstBI TT'CG_AA
Cuts at: 2701 2701
Size: 4340
BstYI r'GATC_y
Cuts at: 1 1365 1832 1855 2190 2436 3448 3534 3546
Size: 1364 467 23 335 246 1012 86 12
Cuts at: 3546 3892 1
Size: 346 449
Fragments arranged by size:
1364 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 738 107
Size: 631 3709
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 336 2290 2471 2634 336
Size: 1954 181 163 2042
Fragments arranged by size:
2042 1954 181 163
BtsI GCAGTG_nn'
Cuts at: 493 937 1583 493
Size: 444 646 3250
Fragments arranged by size:
3250 646 444
DraI TTT'AAA
Cuts at: 1339 1339
Size: 4340
DrdI GACnn_nn'nnGTC
Cuts at: 2046 2904 2046
Size: 858 3482
EaeI y'GGCC_r
Cuts at: 300 1925 2099 2490 2517 3862 300
Size: 1625 174 391 27 1345 778
Fragments arranged by size:
1625 1345 778 391 174 27
EagI C'GGCC_G
Cuts at: 1925 1925
Size: 4340
EarI CTCTTCn'nnn_
Cuts at: 569 844 2363 2573 569
Size: 275 1519 210 2336
Fragments arranged by size:
2336 1519 275 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2548 2858 3004 4150 2548
Size: 310 146 1146 2738
Fragments arranged by size:
2738 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1352 1352
Size: 4340
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 641 825 1315 2165 2597 3344 3612 274
Size: 367 184 490 850 432 747 268 1002
Fragments arranged by size:
1002 850 747 490 432 367 268 184
EcoNI CCTnn'n_nnAGG
Cuts at: 721 721
Size: 4340
EcoO109I rG'GnC_Cy
Cuts at: 1479 1479
Size: 4340
EcoRI G'AATT_C
Cuts at: 1344 1344
Size: 4340
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 204 2359 204
Size: 2155 2185
FspI TGC'GCA
Cuts at: 2121 2121
Size: 4340
HaeII r_GCGC'y
Cuts at: 1774 2022 3044 4023 1774
Size: 248 1022 979 2091
Fragments arranged by size:
2091 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 880 912 2182 2212 2214 2244 880
Size: 32 1270 30 2 30 2976
Fragments arranged by size:
2976 1270 32 30 30 2
HincII GTy'rAC
Cuts at: 1379 1379
Size: 4340
KasI G'GCGC_C
Cuts at: 1770 2018 1770
Size: 248 4092
KpnI G_GTAC'C
Cuts at: 1360 1360
Size: 4340
MluI A'CGCG_T
Cuts at: 3773 3773
Size: 4340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3011 3195 3011
Size: 184 4156
MscI TGG'CCA
Cuts at: 2101 2101
Size: 4340
MslI CAynn'nnrTG
Cuts at: 1024 2329 2456 1024
Size: 1305 127 2908
Fragments arranged by size:
2908 1305 127
MspA1I CmG'CkG
Cuts at: 1451 1765 2125 3138 3383 1451
Size: 314 360 1013 245 2408
Fragments arranged by size:
2408 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2521 2521
Size: 4340
NarI GG'CG_CC
Cuts at: 1771 2019 1771
Size: 248 4092
NcoI C'CATG_G
Cuts at: 107 738 107
Size: 631 3709
NgoMIV G'CCGG_C
Cuts at: 2519 2519
Size: 4340
NspI r_CATG'y
Cuts at: 445 1393 1893 2800 445
Size: 948 500 907 1985
Fragments arranged by size:
1985 948 907 500
PciI A'CATG_T
Cuts at: 2796 2796
Size: 4340
PmeI GTTT'AAAC
Cuts at: 1339 1339
Size: 4340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3511 3479
Size: 32 4308
PshAI GACnn'nnGTC
Cuts at: 130 1182 1204 130
Size: 1052 22 3266
Fragments arranged by size:
3266 1052 22
PspXI vC'TCGA_Gb
Cuts at: 706 706
Size: 4340
PstI C_TGCA'G
Cuts at: 413 500 1387 2072 413
Size: 87 887 685 2681
Fragments arranged by size:
2681 887 685 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4340
PvuII CAG'CTG
Cuts at: 1765 2125 1765
Size: 360 3980
RsrII CG'GwC_CG
Cuts at: 2535 2535
Size: 4340
SacI G_AGCT'C
Cuts at: 1354 1354
Size: 4340
SalI G'TCGA_C
Cuts at: 1377 1377
Size: 4340
SapI GCTCTTCn'nnn_
Cuts at: 2363 2573 2363
Size: 210 4130
SbfI CC_TGCA'GG
Cuts at: 413 1387 413
Size: 974 3366
ScaI AGT'ACT
Cuts at: 3857 3857
Size: 4340
SexAI A'CCwGG_T
Cuts at: 890 890
Size: 4340
SfcI C'TryA_G
Cuts at: 33 218 409 496 1071 1383 2068 3061 3252
Size: 185 191 87 575 312 685 993 191
Cuts at: 3252 33
Size: 1121
Fragments arranged by size:
1121 993 685 575 312 191 191 185
87
SfoI GGC'GCC
Cuts at: 1772 2020 1772
Size: 248 4092
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4340
SmaI CCC'GGG
Cuts at: 123 1362 123
Size: 1239 3101
SmlI C'TyrA_G
Cuts at: 706 988 1100 1495 2410 2902 3164 706
Size: 282 112 395 915 492 262 1882
Fragments arranged by size:
1882 915 492 395 282 262 112
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4340
SphI G_CATG'C
Cuts at: 445 1393 1893 445
Size: 948 500 2892
Fragments arranged by size:
2892 948 500
StuI AGG'CCT
Cuts at: 367 526 367
Size: 159 4181
StyI C'CwwG_G
Cuts at: 107 738 1474 1828 107
Size: 631 736 354 2619
Fragments arranged by size:
2619 736 631 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1923 2056 2515 2724 1923
Size: 133 459 209 3539
Fragments arranged by size:
3539 459 209 133
TatI w'GTAC_w
Cuts at: 170 731 854 923 3855 170
Size: 561 123 69 2932 655
Fragments arranged by size:
2932 655 561 123 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 662 694 1049 1081 3857 3889 662
Size: 32 355 32 2776 32 1113
Fragments arranged by size:
2776 1113 355 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2137 2137
Size: 4340
XbaI T'CTAG_A
Cuts at: 1371 1371
Size: 4340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1835 1835
Size: 4340
XhoI C'TCGA_G
Cuts at: 706 1100 706
Size: 394 3946
XmaI C'CCGG_G
Cuts at: 121 1360 121
Size: 1239 3101
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PshAI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BpmI BsaXI
BseRI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PsiI PspOMI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI