Restriction map for
pF1KB6639
(Circular) (Six-base) MAPSORT of: pF1KB6639.seq Check: 2702 from: 1 to: 4367
pF1KB6639 4367 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AatII G_ACGT'C
Cuts at: 1047 1107 1047
Size: 60 4307
AccI GT'mk_AC
Cuts at: 154 874 1405 154
Size: 720 531 3116
Fragments arranged by size:
3116 720 531
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 671 2192 2624 3371 3639 443
Size: 228 1521 432 747 268 1171
Fragments arranged by size:
1521 1171 747 432 268 228
AfeI AGC'GCT
Cuts at: 796 796
Size: 4367
AflIII A'CryG_T
Cuts at: 1719 2823 3800 1719
Size: 1104 977 2286
Fragments arranged by size:
2286 1104 977
AgeI A'CCGG_T
Cuts at: 598 2755 598
Size: 2157 2210
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1247 1247
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 421 726 1672 3239 421
Size: 305 946 1567 1549
Fragments arranged by size:
1567 1549 946 305
ApaLI G'TGCA_C
Cuts at: 3137 3137
Size: 4367
ApoI r'AATT_y
Cuts at: 829 1371 3720 3976 829
Size: 542 2349 256 1220
Fragments arranged by size:
2349 1220 542 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 1387 1387
Size: 4367
BamHI G'GATC_C
Cuts at: 1392 1392
Size: 4367
BanI G'GyrC_C
Cuts at: 1383 1797 2045 2080 1383
Size: 414 248 35 3670
Fragments arranged by size:
3670 414 248 35
BanII G_rGCy'C
Cuts at: 1381 2411 1381
Size: 1030 3337
BbeI G_GCGC'C
Cuts at: 1801 2049 1801
Size: 248 4119
BbsI GAAGACnn'nnnn_
Cuts at: 431 431
Size: 4367
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 240 1887 240
Size: 1647 2720
BglII A'GATC_T
Cuts at: 1882 3919 1882
Size: 2037 2330
BlpI GC'TnA_GC
Cuts at: 1479 1479
Size: 4367
Bme1580I G_kGCm'C
Cuts at: 1992 2085 3141 1992
Size: 93 1056 3218
Fragments arranged by size:
3218 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1579 1660 1995 4139 1579
Size: 81 335 2144 1807
Fragments arranged by size:
2144 1807 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 740 1094 449
Size: 291 354 3722
Fragments arranged by size:
3722 354 291
Bpu10I CC'TnA_GC
Cuts at: 552 779 552
Size: 227 4140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 555 1543 2422 2914 3212 555
Size: 988 879 492 298 1710
Fragments arranged by size:
1710 988 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 728 4071 728
Size: 3343 1024
BsaAI yAC'GTr
Cuts at: 1720 1720
Size: 4367
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 1044 1104 1798 2046 2748 4244 1044
Size: 60 694 248 702 1496 1167
Fragments arranged by size:
1496 1167 702 694 248 60
BsaWI w'CCGG_w
Cuts at: 598 1556 2077 2755 3029 3176 3587 598
Size: 958 521 678 274 147 411 1378
Fragments arranged by size:
1378 958 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 435 465 611 641 435
Size: 30 146 30 4161
Fragments arranged by size:
4161 146 30 30
BseYI C'CCAG_C
Cuts at: 1792 2550 3127 3623 1792
Size: 758 577 496 2536
Fragments arranged by size:
2536 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 556
Size: 4367
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3163 104
Size: 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1381 2159 2349 3141 1381
Size: 778 190 792 2607
Fragments arranged by size:
2607 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1381 1992 2085 2159 2349 2411 3141 1381
Size: 611 93 74 190 62 730 2607
Fragments arranged by size:
2607 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3587 3587
Size: 4367
BspHI T'CATG_A
Cuts at: 3543 3543
Size: 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1417 1631 1933 2314 1417
Size: 214 302 381 3470
Fragments arranged by size:
3470 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2659 2713 4197 4362 50
Size: 2609 54 1484 165 55
Fragments arranged by size:
2609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 527 2279 527
Size: 1752 2615
BsrFI r'CCGG_y
Cuts at: 123 598 2365 2546 2755 123
Size: 475 1767 181 209 1735
Fragments arranged by size:
1767 1735 475 209 181
BsrGI T'GTAC_A
Cuts at: 524 1200 524
Size: 676 3691
BssSI C'ACGA_G
Cuts at: 2638 2996 2638
Size: 358 4009
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstYI r'GATC_y
Cuts at: 1 1392 1859 1882 2217 2463 3475 3561 3573
Size: 1391 467 23 335 246 1012 86 12
Cuts at: 3573 3919 1
Size: 346 449
Fragments arranged by size:
1391 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 155 875 155
Size: 720 3647
BtgI C'CryG_G
Cuts at: 107 345 107
Size: 238 4129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2317 2498 2661 2317
Size: 181 163 4023
Fragments arranged by size:
4023 181 163
BtsI GCAGTG_nn'
Cuts at: 1610 1610
Size: 4367
ClaI AT'CG_AT
Cuts at: 914 914
Size: 4367
DraI TTT'AAA
Cuts at: 402 1366 402
Size: 964 3403
DrdI GACnn_nn'nnGTC
Cuts at: 2073 2931 2073
Size: 858 3509
EaeI y'GGCC_r
Cuts at: 334 1952 2126 2517 2544 3889 334
Size: 1618 174 391 27 1345 812
Fragments arranged by size:
1618 1345 812 391 174 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 331 2575 2885 3031 4177 331
Size: 2244 310 146 1146 521
Fragments arranged by size:
2244 1146 521 310 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 449 671 740 1094 2192 2624 3371 3639
Size: 6 222 69 354 1098 432 747 268
Cuts at: 3639 443
Size: 1171
Fragments arranged by size:
1171 1098 747 432 354 268 222 69
6
EcoO109I rG'GnC_Cy
Cuts at: 940 1506 940
Size: 566 3801
EcoRI G'AATT_C
Cuts at: 1371 1371
Size: 4367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4367
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 798 1801 2049 3071 4050 798
Size: 1003 248 1022 979 1115
Fragments arranged by size:
1115 1022 1003 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 435 467 611 643 932 964 1034 1066 2209
Size: 32 144 32 289 32 70 32 1143
Cuts at: 2209 2239 2241 2271 435
Size: 30 2 30 2531
Fragments arranged by size:
2531 1143 289 144 70 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 596 1406 596
Size: 810 3557
KasI G'GCGC_C
Cuts at: 1797 2045 1797
Size: 248 4119
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3038 3222 3038
Size: 184 4183
MscI TGG'CCA
Cuts at: 336 2128 336
Size: 1792 2575
MslI CAynn'nnrTG
Cuts at: 532 643 2356 2483 532
Size: 111 1713 127 2416
Fragments arranged by size:
2416 1713 127 111
MspA1I CmG'CkG
Cuts at: 671 1478 1792 2152 3165 3410 671
Size: 807 314 360 1013 245 1628
Fragments arranged by size:
1628 1013 807 360 314 245
NaeI GCC'GGC
Cuts at: 125 2548 125
Size: 2423 1944
NarI GG'CG_CC
Cuts at: 1798 2046 1798
Size: 248 4119
NcoI C'CATG_G
Cuts at: 107 345 107
Size: 238 4129
NdeI CA'TA_TG
Cuts at: 321 321
Size: 4367
NgoMIV G'CCGG_C
Cuts at: 123 2546 123
Size: 2423 1944
NspI r_CATG'y
Cuts at: 1224 1420 1920 2827 1224
Size: 196 500 907 2764
Fragments arranged by size:
2764 907 500 196
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PflMI CCAn_nnn'nTGG
Cuts at: 1348 1348
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PpuMI rG'GwC_Cy
Cuts at: 940 940
Size: 4367
PstI C_TGCA'G
Cuts at: 1414 2099 1414
Size: 685 3682
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 671 1792 2152 671
Size: 1121 360 2886
Fragments arranged by size:
2886 1121 360
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SfcI C'TryA_G
Cuts at: 33 798 1133 1410 2095 3088 3279 33
Size: 765 335 277 685 993 191 1121
Fragments arranged by size:
1121 993 765 685 335 277 191
SfoI GGC'GCC
Cuts at: 1799 2047 1799
Size: 248 4119
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 570 1522 2437 2929 3191 570
Size: 952 915 492 262 1746
Fragments arranged by size:
1746 952 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
SspI AAT'ATT
Cuts at: 406 1062 406
Size: 656 3711
StyI C'CwwG_G
Cuts at: 107 345 767 1501 1855 107
Size: 238 422 734 354 2619
Fragments arranged by size:
2619 734 422 354 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 226 752 1950 2083 2542 2751 226
Size: 526 1198 133 459 209 1842
Fragments arranged by size:
1842 1198 526 459 209 133
TatI w'GTAC_w
Cuts at: 524 1200 3882 524
Size: 676 2682 1009
Fragments arranged by size:
2682 1009 676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3884 3916 3884
Size: 32 4335
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 1862
Size: 4367
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
XmnI GAAnn'nnTTC
Cuts at: 1016 1016
Size: 4367
ZraI GAC'GTC
Cuts at: 1045 1105 1045
Size: 60 4307
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BseRI
BsiWI BsmI BsmBI BssHII BstEII BstXI Bsu36I CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI