Restriction map for
pF1KB6636
(Circular) (Six-base) MAPSORT of: pF1KB6636.seq Check: 6962 from: 1 to: 4436
pF1KB6636 4436 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 1474 1474
Size: 4436
Acc65I G'GTAC_C
Cuts at: 1452 1452
Size: 4436
AclI AA'CG_TT
Cuts at: 4276 4276
Size: 4436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 572 664 761 1130 2261 2693 3440 3708
Size: 79 92 97 369 1131 432 747 268
Cuts at: 3708 493
Size: 1221
Fragments arranged by size:
1221 1131 747 432 369 268 97 92
79
AflIII A'CryG_T
Cuts at: 1788 2892 3869 1788
Size: 1104 977 2355
Fragments arranged by size:
2355 1104 977
AgeI A'CCGG_T
Cuts at: 2824 2824
Size: 4436
AleI CACnn'nnGTG
Cuts at: 843 845 843
Size: 2 4434
AlwNI CAG_nnn'CTG
Cuts at: 220 1741 3308 220
Size: 1521 1567 1348
Fragments arranged by size:
1567 1521 1348
ApaLI G'TGCA_C
Cuts at: 1379 3206 1379
Size: 1827 2609
ApoI r'AATT_y
Cuts at: 518 1089 1169 1440 3789 4045 518
Size: 571 80 271 2349 256 909
Fragments arranged by size:
2349 909 571 271 256 80
AseI AT'TA_AT
Cuts at: 20 20
Size: 4436
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
AvaI C'yCGr_G
Cuts at: 1456 1456
Size: 4436
BamHI G'GATC_C
Cuts at: 477 1461 477
Size: 984 3452
BanI G'GyrC_C
Cuts at: 1452 1866 2114 2149 1452
Size: 414 248 35 3739
Fragments arranged by size:
3739 414 248 35
BanII G_rGCy'C
Cuts at: 1450 2480 1450
Size: 1030 3406
BbeI G_GCGC'C
Cuts at: 1870 2118 1870
Size: 248 4188
BbsI GAAGACnn'nnnn_
Cuts at: 713 767 1135 713
Size: 54 368 4014
Fragments arranged by size:
4014 368 54
BbvCI CC'TCA_GC
Cuts at: 736 736
Size: 4436
BciVI GTATCCnnnnn_n'
Cuts at: 1029 2334 3095 4436 1029
Size: 1305 761 1341 1029
Fragments arranged by size:
1341 1305 1029 761
BclI T'GATC_A
Cuts at: 1956 1956
Size: 4436
BglII A'GATC_T
Cuts at: 1951 3988 1951
Size: 2037 2399
BlpI GC'TnA_GC
Cuts at: 866 1548 866
Size: 682 3754
Bme1580I G_kGCm'C
Cuts at: 1383 2061 2154 3210 1383
Size: 678 93 1056 2609
Fragments arranged by size:
2609 1056 678 93
BmrI ACTGGGnnnn_n'
Cuts at: 1648 1729 2064 4208 1648
Size: 81 335 2144 1876
Fragments arranged by size:
2144 1876 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2803 2803
Size: 4436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 503 550 242
Size: 261 47 4128
Fragments arranged by size:
4128 261 47
Bpu10I CC'TnA_GC
Cuts at: 736 736
Size: 4436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 1612 2491 2983 3281 935
Size: 677 879 492 298 2090
Fragments arranged by size:
2090 879 677 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4140 4140
Size: 4436
BsaAI yAC'GTr
Cuts at: 1789 1789
Size: 4436
BsaBI GATnn'nnATC
Cuts at: 6 1950 3987 6
Size: 1944 2037 455
Fragments arranged by size:
2037 1944 455
BsaHI Gr'CG_yC
Cuts at: 1867 2115 2817 4313 1867
Size: 248 702 1496 1990
Fragments arranged by size:
1990 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1625 2146 2824 3098 3245 3656 1625
Size: 521 678 274 147 411 2405
Fragments arranged by size:
2405 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 168 198 476 506 168
Size: 30 278 30 4098
Fragments arranged by size:
4098 278 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 865 865
Size: 4436
BseYI C'CCAG_C
Cuts at: 1861 2619 3196 3692 1861
Size: 758 577 496 2605
Fragments arranged by size:
2605 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1978 2024 3232 104
Size: 1874 46 1208 1308
Fragments arranged by size:
1874 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1383 1450 2228 2418 3210 1383
Size: 67 778 190 792 2609
Fragments arranged by size:
2609 792 778 190 67
Bsp1286I G_dGCh'C
Cuts at: 1383 1450 2061 2154 2228 2418 2480 3210 1383
Size: 67 611 93 74 190 62 730 2609
Fragments arranged by size:
2609 730 611 190 93 74 67 62
BspEI T'CCGG_A
Cuts at: 3656 3656
Size: 4436
BspHI T'CATG_A
Cuts at: 3612 3612
Size: 4436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1486 1700 2002 2383 1486
Size: 214 302 381 3539
Fragments arranged by size:
3539 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2728 2782 4266 4431 50
Size: 2678 54 1484 165 55
Fragments arranged by size:
2678 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1052 2348 1052
Size: 1296 3140
BsrFI r'CCGG_y
Cuts at: 2434 2615 2824 2434
Size: 181 209 4046
Fragments arranged by size:
4046 209 181
BssSI C'ACGA_G
Cuts at: 2707 3065 2707
Size: 358 4078
BstAPI GCAn_nnn'nTGC
Cuts at: 1813 1813
Size: 4436
BstBI TT'CG_AA
Cuts at: 2797 2797
Size: 4436
BstYI r'GATC_y
Cuts at: 1 477 1461 1928 1951 2286 2532 3544 3630
Size: 476 984 467 23 335 246 1012 86
Cuts at: 3630 3642 3988 1
Size: 12 346 449
Fragments arranged by size:
1012 984 476 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4436
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2386 2567 2730 2386
Size: 181 163 4092
Fragments arranged by size:
4092 181 163
BtsI GCAGTG_nn'
Cuts at: 1277 1679 1277
Size: 402 4034
DraI TTT'AAA
Cuts at: 1064 1435 1064
Size: 371 4065
DrdI GACnn_nn'nnGTC
Cuts at: 2142 3000 2142
Size: 858 3578
EaeI y'GGCC_r
Cuts at: 2021 2195 2586 2613 3958 2021
Size: 174 391 27 1345 2499
Fragments arranged by size:
2499 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2021 2021
Size: 4436
EarI CTCTTCn'nnn_
Cuts at: 279 300 1229 1259 2459 2669 279
Size: 21 929 30 1200 210 2046
Fragments arranged by size:
2046 1200 929 210 30 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1327 2644 2954 3100 4246 1327
Size: 1317 310 146 1146 1517
Fragments arranged by size:
1517 1317 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1448 1448
Size: 4436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 493 503 550 572 664 761 1130 2261
Size: 251 10 47 22 92 97 369 1131
Cuts at: 2261 2693 3440 3708 242
Size: 432 747 268 970
Fragments arranged by size:
1131 970 747 432 369 268 251 97
92 47 22 10
EcoO109I rG'GnC_Cy
Cuts at: 1575 1575
Size: 4436
EcoRI G'AATT_C
Cuts at: 1440 1440
Size: 4436
EcoRV GAT'ATC
Cuts at: 405 405
Size: 4436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 4436
FspI TGC'GCA
Cuts at: 2217 2217
Size: 4436
HaeII r_GCGC'y
Cuts at: 1870 2118 3140 4119 1870
Size: 248 1022 979 2187
Fragments arranged by size:
2187 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 571 603 2278 2308 2310 2340 571
Size: 32 1675 30 2 30 2667
Fragments arranged by size:
2667 1675 32 30 30 2
HincII GTy'rAC
Cuts at: 1422 1475 1422
Size: 53 4383
HindIII A'AGCT_T
Cuts at: 932 1030 932
Size: 98 4338
KasI G'GCGC_C
Cuts at: 1866 2114 1866
Size: 248 4188
KpnI G_GTAC'C
Cuts at: 1456 1456
Size: 4436
MfeI C'AATT_G
Cuts at: 452 824 452
Size: 372 4064
MluI A'CGCG_T
Cuts at: 3869 3869
Size: 4436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3107 3291 3107
Size: 184 4252
MscI TGG'CCA
Cuts at: 2197 2197
Size: 4436
MslI CAynn'nnrTG
Cuts at: 745 843 845 2425 2552 745
Size: 98 2 1580 127 2629
Fragments arranged by size:
2629 1580 127 98 2
MspA1I CmG'CkG
Cuts at: 1024 1547 1861 2221 3234 3479 1024
Size: 523 314 360 1013 245 1981
Fragments arranged by size:
1981 1013 523 360 314 245
NaeI GCC'GGC
Cuts at: 2617 2617
Size: 4436
NarI GG'CG_CC
Cuts at: 1867 2115 1867
Size: 248 4188
NcoI C'CATG_G
Cuts at: 107 107
Size: 4436
NgoMIV G'CCGG_C
Cuts at: 2615 2615
Size: 4436
NspI r_CATG'y
Cuts at: 334 1108 1141 1489 1989 2896 334
Size: 774 33 348 500 907 1874
Fragments arranged by size:
1874 907 774 500 348 33
PciI A'CATG_T
Cuts at: 2892 2892
Size: 4436
PfoI T'CCnGG_A
Cuts at: 480 480
Size: 4436
PmeI GTTT'AAAC
Cuts at: 1435 1435
Size: 4436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1355 1387 3575 3607 1355
Size: 32 2188 32 2184
Fragments arranged by size:
2188 2184 32 32
PstI C_TGCA'G
Cuts at: 216 773 1483 2168 216
Size: 557 710 685 2484
Fragments arranged by size:
2484 710 685 557
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4436
PvuII CAG'CTG
Cuts at: 1861 2221 1861
Size: 360 4076
RsrII CG'GwC_CG
Cuts at: 2631 2631
Size: 4436
SacI G_AGCT'C
Cuts at: 1450 1450
Size: 4436
SalI G'TCGA_C
Cuts at: 1473 1473
Size: 4436
SapI GCTCTTCn'nnn_
Cuts at: 1229 2459 2669 1229
Size: 1230 210 2996
Fragments arranged by size:
2996 1230 210
SbfI CC_TGCA'GG
Cuts at: 1483 1483
Size: 4436
ScaI AGT'ACT
Cuts at: 3953 3953
Size: 4436
SfcI C'TryA_G
Cuts at: 33 212 769 1479 2164 3157 3348 33
Size: 179 557 710 685 993 191 1121
Fragments arranged by size:
1121 993 710 685 557 191 179
SfoI GGC'GCC
Cuts at: 1868 2116 1868
Size: 248 4188
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4436
SmaI CCC'GGG
Cuts at: 1458 1458
Size: 4436
SmlI C'TyrA_G
Cuts at: 950 1591 2506 2998 3260 950
Size: 641 915 492 262 2126
Fragments arranged by size:
2126 915 641 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4436
SphI G_CATG'C
Cuts at: 1108 1489 1989 1108
Size: 381 500 3555
Fragments arranged by size:
3555 500 381
SspI AAT'ATT
Cuts at: 968 968
Size: 4436
StyI C'CwwG_G
Cuts at: 107 196 1570 1924 107
Size: 89 1374 354 2619
Fragments arranged by size:
2619 1374 354 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2019 2152 2611 2820 2019
Size: 133 459 209 3635
Fragments arranged by size:
3635 459 209 133
TatI w'GTAC_w
Cuts at: 255 812 3951 255
Size: 557 3139 740
Fragments arranged by size:
3139 740 557
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 262 294 3953 3985 262
Size: 32 3659 32 713
Fragments arranged by size:
3659 713 32 32
Tth111I GACn'n_nGTC
Cuts at: 2233 2233
Size: 4436
XbaI T'CTAG_A
Cuts at: 1467 1467
Size: 4436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1931 1931
Size: 4436
XmaI C'CCGG_G
Cuts at: 1456 1456
Size: 4436
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI