Restriction map for
pF1KB6629
(Circular) (Six-base) MAPSORT of: pF1KB6629.seq Check: 6741 from: 1 to: 4358
pF1KB6629 4358 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1396 1396
Size: 4358
Acc65I G'GTAC_C
Cuts at: 131 1374 131
Size: 1243 3115
AclI AA'CG_TT
Cuts at: 4198 4198
Size: 4358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 1318 2183 2615 3362 3630 775
Size: 543 865 432 747 268 1503
Fragments arranged by size:
1503 865 747 543 432 268
AflIII A'CryG_T
Cuts at: 965 1710 2814 3791 965
Size: 745 1104 977 1532
Fragments arranged by size:
1532 1104 977 745
AgeI A'CCGG_T
Cuts at: 2746 2746
Size: 4358
AhdI GACnn_n'nnGTC
Cuts at: 696 696
Size: 4358
AlwNI CAG_nnn'CTG
Cuts at: 753 1663 3230 753
Size: 910 1567 1881
Fragments arranged by size:
1881 1567 910
ApaI G_GGCC'C
Cuts at: 1239 1239
Size: 4358
ApaLI G'TGCA_C
Cuts at: 1345 3128 1345
Size: 1783 2575
ApoI r'AATT_y
Cuts at: 403 847 864 988 1362 3711 3967 403
Size: 444 17 124 374 2349 256 794
Fragments arranged by size:
2349 794 444 374 256 124 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 4358
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
AvaI C'yCGr_G
Cuts at: 1378 1378
Size: 4358
BamHI G'GATC_C
Cuts at: 1383 1383
Size: 4358
BanI G'GyrC_C
Cuts at: 131 994 1374 1788 2036 2071 131
Size: 863 380 414 248 35 2418
Fragments arranged by size:
2418 863 414 380 248 35
BanII G_rGCy'C
Cuts at: 736 1239 1372 2402 736
Size: 503 133 1030 2692
Fragments arranged by size:
2692 1030 503 133
BbeI G_GCGC'C
Cuts at: 1792 2040 1792
Size: 248 4110
BbsI GAAGACnn'nnnn_
Cuts at: 1324 1324
Size: 4358
BbvCI CC'TCA_GC
Cuts at: 248 248
Size: 4358
BciVI GTATCCnnnnn_n'
Cuts at: 2256 3017 4358 2256
Size: 761 1341 2256
Fragments arranged by size:
2256 1341 761
BclI T'GATC_A
Cuts at: 443 1878 443
Size: 1435 2923
BglI GCCn_nnn'nGGC
Cuts at: 1003 1003
Size: 4358
BglII A'GATC_T
Cuts at: 1873 3910 1873
Size: 2037 2321
BlpI GC'TnA_GC
Cuts at: 1470 1470
Size: 4358
Bme1580I G_kGCm'C
Cuts at: 1239 1349 1983 2076 3132 1239
Size: 110 634 93 1056 2465
Fragments arranged by size:
2465 1056 634 110 93
BmrI ACTGGGnnnn_n'
Cuts at: 1570 1651 1986 4130 1570
Size: 81 335 2144 1798
Fragments arranged by size:
2144 1798 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 4358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 381 394 381
Size: 13 4345
Bpu10I CC'TnA_GC
Cuts at: 248 248
Size: 4358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1534 2413 2905 3203 1534
Size: 879 492 298 2689
Fragments arranged by size:
2689 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4062 4062
Size: 4358
BsaAI yAC'GTr
Cuts at: 711 966 1711 711
Size: 255 745 3358
Fragments arranged by size:
3358 745 255
BsaBI GATnn'nnATC
Cuts at: 6 1872 3909 6
Size: 1866 2037 455
Fragments arranged by size:
2037 1866 455
BsaHI Gr'CG_yC
Cuts at: 1016 1789 2037 2739 4235 1016
Size: 773 248 702 1496 1139
Fragments arranged by size:
1496 1139 773 702 248
BsaWI w'CCGG_w
Cuts at: 926 1547 2068 2746 3020 3167 3578 926
Size: 621 521 678 274 147 411 1706
Fragments arranged by size:
1706 678 621 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 194 224 379 409 194
Size: 30 155 30 4143
Fragments arranged by size:
4143 155 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 305 1057 1242 305
Size: 752 185 3421
Fragments arranged by size:
3421 752 185
BseYI C'CCAG_C
Cuts at: 138 360 1783 2541 3118 3614 138
Size: 222 1423 758 577 496 882
Fragments arranged by size:
1423 882 758 577 496 222
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1210 1210
Size: 4358
BsiEI CG_ry'CG
Cuts at: 104 1104 1900 1946 3154 104
Size: 1000 796 46 1208 1308
Fragments arranged by size:
1308 1208 1000 796 46
BsiHKAI G_wGCw'C
Cuts at: 1349 1372 2150 2340 3132 1349
Size: 23 778 190 792 2575
Fragments arranged by size:
2575 792 778 190 23
BsmBI CGTCTCn'nnnn_
Cuts at: 1191 1191
Size: 4358
Bsp1286I G_dGCh'C
Cuts at: 736 1239 1349 1372 1983 2076 2150 2340 2402
Size: 503 110 23 611 93 74 190 62
Cuts at: 2402 3132 736
Size: 730 1962
Fragments arranged by size:
1962 730 611 503 190 110 93 74
62 23
BspEI T'CCGG_A
Cuts at: 926 3578 926
Size: 2652 1706
BspHI T'CATG_A
Cuts at: 3534 3534
Size: 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 920 1408 1622 1924 2305 920
Size: 488 214 302 381 2973
Fragments arranged by size:
2973 488 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1023 1047 1206 2650 2704 4188 4353 50
Size: 973 24 159 1444 54 1484 165 55
Fragments arranged by size:
1484 1444 973 165 159 55 54 24
BsrDI GCAATG_nn'
Cuts at: 191 2270 191
Size: 2079 2279
BsrFI r'CCGG_y
Cuts at: 134 2356 2537 2746 134
Size: 2222 181 209 1746
Fragments arranged by size:
2222 1746 209 181
BsrGI T'GTAC_A
Cuts at: 542 542
Size: 4358
BssSI C'ACGA_G
Cuts at: 2629 2987 2629
Size: 358 4000
BstAPI GCAn_nnn'nTGC
Cuts at: 1735 1735
Size: 4358
BstBI TT'CG_AA
Cuts at: 2719 2719
Size: 4358
BstYI r'GATC_y
Cuts at: 1 1383 1850 1873 2208 2454 3466 3552 3564
Size: 1382 467 23 335 246 1012 86 12
Cuts at: 3564 3910 1
Size: 346 449
Fragments arranged by size:
1382 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1077 1230 107
Size: 970 153 3235
Fragments arranged by size:
3235 970 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 925 2308 2489 2652 925
Size: 1383 181 163 2631
Fragments arranged by size:
2631 1383 181 163
BtsI GCAGTG_nn'
Cuts at: 1601 1601
Size: 4358
DraI TTT'AAA
Cuts at: 1357 1357
Size: 4358
DrdI GACnn_nn'nnGTC
Cuts at: 340 2064 2922 340
Size: 1724 858 1776
Fragments arranged by size:
1776 1724 858
EaeI y'GGCC_r
Cuts at: 563 1101 1943 2117 2508 2535 3880 563
Size: 538 842 174 391 27 1345 1041
Fragments arranged by size:
1345 1041 842 538 391 174 27
EagI C'GGCC_G
Cuts at: 1101 1943 1101
Size: 842 3516
EarI CTCTTCn'nnn_
Cuts at: 200 388 678 2381 2591 200
Size: 188 290 1703 210 1967
Fragments arranged by size:
1967 1703 290 210 188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2566 2876 3022 4168 2566
Size: 310 146 1146 2756
Fragments arranged by size:
2756 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1370 1370
Size: 4358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 381 394 775 1318 2183 2615 3362 3630 381
Size: 13 381 543 865 432 747 268 1109
Fragments arranged by size:
1109 865 747 543 432 381 268 13
EcoO109I rG'GnC_Cy
Cuts at: 378 1235 1236 1497 378
Size: 857 1 261 3239
Fragments arranged by size:
3239 857 261 1
EcoRI G'AATT_C
Cuts at: 1362 1362
Size: 4358
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4358
FspI TGC'GCA
Cuts at: 2139 2139
Size: 4358
HaeII r_GCGC'y
Cuts at: 1792 2040 3062 4041 1792
Size: 248 1022 979 2109
Fragments arranged by size:
2109 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 284 316 682 714 1296 1328 2200 2230 2232
Size: 32 366 32 582 32 872 30 2
Cuts at: 2232 2262 284
Size: 30 2380
Fragments arranged by size:
2380 872 582 366 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1245 1397 1245
Size: 152 4206
HindIII A'AGCT_T
Cuts at: 220 220
Size: 4358
KasI G'GCGC_C
Cuts at: 1788 2036 1788
Size: 248 4110
KpnI G_GTAC'C
Cuts at: 135 1378 135
Size: 1243 3115
MluI A'CGCG_T
Cuts at: 3791 3791
Size: 4358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1283 3029 3213 1283
Size: 1746 184 2428
Fragments arranged by size:
2428 1746 184
MscI TGG'CCA
Cuts at: 565 2119 565
Size: 1554 2804
MslI CAynn'nnrTG
Cuts at: 349 488 820 2347 2474 349
Size: 139 332 1527 127 2233
Fragments arranged by size:
2233 1527 332 139 127
MspA1I CmG'CkG
Cuts at: 158 1079 1232 1469 1783 2143 3156 3401 158
Size: 921 153 237 314 360 1013 245 1115
Fragments arranged by size:
1115 1013 921 360 314 245 237 153
NaeI GCC'GGC
Cuts at: 2539 2539
Size: 4358
NarI GG'CG_CC
Cuts at: 1789 2037 1789
Size: 248 4110
NcoI C'CATG_G
Cuts at: 107 107
Size: 4358
NgoMIV G'CCGG_C
Cuts at: 2537 2537
Size: 4358
NspI r_CATG'y
Cuts at: 984 1293 1411 1911 2818 984
Size: 309 118 500 907 2524
Fragments arranged by size:
2524 907 500 309 118
PciI A'CATG_T
Cuts at: 2814 2814
Size: 4358
PflMI CCAn_nnn'nTGG
Cuts at: 314 314
Size: 4358
PfoI T'CCnGG_A
Cuts at: 397 397
Size: 4358
PmeI GTTT'AAAC
Cuts at: 1357 1357
Size: 4358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 4326
PpuMI rG'GwC_Cy
Cuts at: 378 378
Size: 4358
PspOMI G'GGCC_C
Cuts at: 1235 1235
Size: 4358
PstI C_TGCA'G
Cuts at: 201 1405 2090 201
Size: 1204 685 2469
Fragments arranged by size:
2469 1204 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4358
PvuII CAG'CTG
Cuts at: 1783 2143 1783
Size: 360 3998
RsrII CG'GwC_CG
Cuts at: 2553 2553
Size: 4358
SacI G_AGCT'C
Cuts at: 1372 1372
Size: 4358
SacII CC_GC'GG
Cuts at: 1080 1233 1080
Size: 153 4205
SalI G'TCGA_C
Cuts at: 1395 1395
Size: 4358
SapI GCTCTTCn'nnn_
Cuts at: 2381 2591 2381
Size: 210 4148
SbfI CC_TGCA'GG
Cuts at: 1405 1405
Size: 4358
ScaI AGT'ACT
Cuts at: 3875 3875
Size: 4358
SexAI A'CCwGG_T
Cuts at: 536 536
Size: 4358
SfcI C'TryA_G
Cuts at: 33 197 1401 2086 3079 3270 33
Size: 164 1204 685 993 191 1121
Fragments arranged by size:
1204 1121 993 685 191 164
SfoI GGC'GCC
Cuts at: 1790 2038 1790
Size: 248 4110
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
SmaI CCC'GGG
Cuts at: 1380 1380
Size: 4358
SmlI C'TyrA_G
Cuts at: 1513 2428 2920 3182 1513
Size: 915 492 262 2689
Fragments arranged by size:
2689 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4358
SphI G_CATG'C
Cuts at: 1411 1911 1411
Size: 500 3858
StyI C'CwwG_G
Cuts at: 107 663 1492 1846 107
Size: 556 829 354 2619
Fragments arranged by size:
2619 829 556 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 604 1052 1941 2074 2533 2742 604
Size: 448 889 133 459 209 2220
Fragments arranged by size:
2220 889 459 448 209 133
TatI w'GTAC_w
Cuts at: 542 719 3873 542
Size: 177 3154 1027
Fragments arranged by size:
3154 1027 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3875 3907 3875
Size: 32 4326
Tth111I GACn'n_nGTC
Cuts at: 2155 2155
Size: 4358
XbaI T'CTAG_A
Cuts at: 1389 1389
Size: 4358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1853 1853
Size: 4358
XmaI C'CCGG_G
Cuts at: 1378 1378
Size: 4358
XmnI GAAnn'nnTTC
Cuts at: 461 934 461
Size: 473 3885
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI