Restriction map for
pF1KB6622
(Circular) (Six-base) MAPSORT of: pF1KB6622.seq Check: 2526 from: 1 to: 4421
pF1KB6622 4421 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1459 1459
Size: 4421
Acc65I G'GTAC_C
Cuts at: 1437 1437
Size: 4421
AclI AA'CG_TT
Cuts at: 4261 4261
Size: 4421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1265 2246 2678 3425 3693 1265
Size: 981 432 747 268 1993
Fragments arranged by size:
1993 981 747 432 268
AflII C'TTAA_G
Cuts at: 246 246
Size: 4421
AflIII A'CryG_T
Cuts at: 685 1773 2877 3854 685
Size: 1088 1104 977 1252
Fragments arranged by size:
1252 1104 1088 977
AgeI A'CCGG_T
Cuts at: 2809 2809
Size: 4421
AleI CACnn'nnGTG
Cuts at: 248 248
Size: 4421
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 949 949
Size: 4421
AlwNI CAG_nnn'CTG
Cuts at: 1726 3293 1726
Size: 1567 2854
ApaLI G'TGCA_C
Cuts at: 3191 3191
Size: 4421
ApoI r'AATT_y
Cuts at: 173 586 762 1287 1425 3774 4030 173
Size: 413 176 525 138 2349 256 564
Fragments arranged by size:
2349 564 525 413 256 176 138
AseI AT'TA_AT
Cuts at: 20 1258 20
Size: 1238 3183
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
AvaI C'yCGr_G
Cuts at: 311 1441 311
Size: 1130 3291
BamHI G'GATC_C
Cuts at: 1446 1446
Size: 4421
BanI G'GyrC_C
Cuts at: 1437 1851 2099 2134 1437
Size: 414 248 35 3724
Fragments arranged by size:
3724 414 248 35
BanII G_rGCy'C
Cuts at: 1435 2465 1435
Size: 1030 3391
BbeI G_GCGC'C
Cuts at: 1855 2103 1855
Size: 248 4173
BciVI GTATCCnnnnn_n'
Cuts at: 2319 3080 4421 2319
Size: 761 1341 2319
Fragments arranged by size:
2319 1341 761
BclI T'GATC_A
Cuts at: 1941 1941
Size: 4421
BglII A'GATC_T
Cuts at: 1008 1936 3973 1008
Size: 928 2037 1456
Fragments arranged by size:
2037 1456 928
BlpI GC'TnA_GC
Cuts at: 1533 1533
Size: 4421
Bme1580I G_kGCm'C
Cuts at: 2046 2139 3195 2046
Size: 93 1056 3272
Fragments arranged by size:
3272 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 559 1633 1714 2049 4193 559
Size: 1074 81 335 2144 787
Fragments arranged by size:
2144 1074 787 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 306 2788 306
Size: 2482 1939
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1597 2476 2968 3266 1597
Size: 879 492 298 2752
Fragments arranged by size:
2752 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4125 4125
Size: 4421
BsaAI yAC'GTr
Cuts at: 181 1774 181
Size: 1593 2828
BsaBI GATnn'nnATC
Cuts at: 6 1935 3972 6
Size: 1929 2037 455
Fragments arranged by size:
2037 1929 455
BsaHI Gr'CG_yC
Cuts at: 1852 2100 2802 4298 1852
Size: 248 702 1496 1975
Fragments arranged by size:
1975 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1610 2131 2809 3083 3230 3641 1610
Size: 521 678 274 147 411 2390
Fragments arranged by size:
2390 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 215 245 473 503 215
Size: 30 228 30 4133
Fragments arranged by size:
4133 228 30 30
BseYI C'CCAG_C
Cuts at: 1846 2604 3181 3677 1846
Size: 758 577 496 2590
Fragments arranged by size:
2590 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 638 638
Size: 4421
BsiEI CG_ry'CG
Cuts at: 104 1963 2009 3217 104
Size: 1859 46 1208 1308
Fragments arranged by size:
1859 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1435 2213 2403 3195 1435
Size: 778 190 792 2661
Fragments arranged by size:
2661 792 778 190
BsmI GAATG_Cn'
Cuts at: 1350 1350
Size: 4421
Bsp1286I G_dGCh'C
Cuts at: 1435 2046 2139 2213 2403 2465 3195 1435
Size: 611 93 74 190 62 730 2661
Fragments arranged by size:
2661 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3641 3641
Size: 4421
BspHI T'CATG_A
Cuts at: 1223 3597 1223
Size: 2374 2047
BspMI ACCTGCnnnn'nnnn_
Cuts at: 825 941 1471 1685 1987 2368 825
Size: 116 530 214 302 381 2878
Fragments arranged by size:
2878 530 381 302 214 116
BsrBI CCG'CTC
Cuts at: 50 2713 2767 4251 4416 50
Size: 2663 54 1484 165 55
Fragments arranged by size:
2663 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1106 2333 1106
Size: 1227 3194
BsrFI r'CCGG_y
Cuts at: 743 2419 2600 2809 743
Size: 1676 181 209 2355
Fragments arranged by size:
2355 1676 209 181
BsrGI T'GTAC_A
Cuts at: 1059 1059
Size: 4421
BssSI C'ACGA_G
Cuts at: 2692 3050 2692
Size: 358 4063
BstAPI GCAn_nnn'nTGC
Cuts at: 1798 1798
Size: 4421
BstBI TT'CG_AA
Cuts at: 2782 2782
Size: 4421
BstEII G'GTnAC_C
Cuts at: 544 544
Size: 4421
BstYI r'GATC_y
Cuts at: 1 1008 1446 1913 1936 2271 2517 3529 3615
Size: 1007 438 467 23 335 246 1012 86
Cuts at: 3615 3627 3973 1
Size: 12 346 449
Fragments arranged by size:
1012 1007 467 449 438 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 276 1268 276
Size: 992 3429
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 968 2371 2552 2715 968
Size: 1403 181 163 2674
Fragments arranged by size:
2674 1403 181 163
BtsI GCAGTG_nn'
Cuts at: 1393 1664 1393
Size: 271 4150
DraI TTT'AAA
Cuts at: 536 798 968 1112 1420 536
Size: 262 170 144 308 3537
Fragments arranged by size:
3537 308 262 170 144
DraIII CAC_nnn'GTG
Cuts at: 152 152
Size: 4421
DrdI GACnn_nn'nnGTC
Cuts at: 2127 2985 2127
Size: 858 3563
EaeI y'GGCC_r
Cuts at: 2006 2180 2571 2598 3943 2006
Size: 174 391 27 1345 2484
Fragments arranged by size:
2484 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2006 2006
Size: 4421
EarI CTCTTCn'nnn_
Cuts at: 1124 2444 2654 1124
Size: 1320 210 2891
Fragments arranged by size:
2891 1320 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2629 2939 3085 4231 2629
Size: 310 146 1146 2819
Fragments arranged by size:
2819 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1433 1433
Size: 4421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1265 2246 2678 3425 3693 1265
Size: 981 432 747 268 1993
Fragments arranged by size:
1993 981 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 160 160
Size: 4421
EcoO109I rG'GnC_Cy
Cuts at: 1560 1560
Size: 4421
EcoRI G'AATT_C
Cuts at: 762 1425 762
Size: 663 3758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 362 2440 362
Size: 2078 2343
FspI TGC'GCA
Cuts at: 2202 2202
Size: 4421
HaeII r_GCGC'y
Cuts at: 1855 2103 3125 4104 1855
Size: 248 1022 979 2172
Fragments arranged by size:
2172 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2263 2293 2295 2325 2263
Size: 30 2 30 4359
Fragments arranged by size:
4359 30 30 2
HincII GTy'rAC
Cuts at: 1460 1460
Size: 4421
HindIII A'AGCT_T
Cuts at: 498 530 498
Size: 32 4389
KasI G'GCGC_C
Cuts at: 1851 2099 1851
Size: 248 4173
KpnI G_GTAC'C
Cuts at: 1441 1441
Size: 4421
MluI A'CGCG_T
Cuts at: 3854 3854
Size: 4421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 459 3092 3276 459
Size: 2633 184 1604
Fragments arranged by size:
2633 1604 184
MscI TGG'CCA
Cuts at: 2182 2182
Size: 4421
MslI CAynn'nnrTG
Cuts at: 248 404 1112 2410 2537 248
Size: 156 708 1298 127 2132
Fragments arranged by size:
2132 1298 708 156 127
MspA1I CmG'CkG
Cuts at: 1308 1532 1846 2206 3219 3464 1308
Size: 224 314 360 1013 245 2265
Fragments arranged by size:
2265 1013 360 314 245 224
NaeI GCC'GGC
Cuts at: 2602 2602
Size: 4421
NarI GG'CG_CC
Cuts at: 1852 2100 1852
Size: 248 4173
NcoI C'CATG_G
Cuts at: 276 1268 276
Size: 992 3429
NgoMIV G'CCGG_C
Cuts at: 2600 2600
Size: 4421
NspI r_CATG'y
Cuts at: 689 951 1474 1974 2881 689
Size: 262 523 500 907 2229
Fragments arranged by size:
2229 907 523 500 262
PciI A'CATG_T
Cuts at: 685 2877 685
Size: 2192 2229
PmeI GTTT'AAAC
Cuts at: 1420 1420
Size: 4421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3560 3592 3560
Size: 32 4389
PsiI TTA'TAA
Cuts at: 1093 1202 1093
Size: 109 4312
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 380 412 380
Size: 32 4389
PstI C_TGCA'G
Cuts at: 419 822 1355 1468 2153 419
Size: 403 533 113 685 2687
Fragments arranged by size:
2687 685 533 403 113
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4421
PvuII CAG'CTG
Cuts at: 1308 1846 2206 1308
Size: 538 360 3523
Fragments arranged by size:
3523 538 360
RsrII CG'GwC_CG
Cuts at: 2616 2616
Size: 4421
SacI G_AGCT'C
Cuts at: 1435 1435
Size: 4421
SalI G'TCGA_C
Cuts at: 1458 1458
Size: 4421
SapI GCTCTTCn'nnn_
Cuts at: 1124 2444 2654 1124
Size: 1320 210 2891
Fragments arranged by size:
2891 1320 210
SbfI CC_TGCA'GG
Cuts at: 1468 1468
Size: 4421
ScaI AGT'ACT
Cuts at: 902 3938 902
Size: 3036 1385
SfcI C'TryA_G
Cuts at: 33 415 818 904 1351 1373 1464 2149 3142
Size: 382 403 86 447 22 91 685 993
Cuts at: 3142 3333 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 447 403 382 191 91
86 22
SfoI GGC'GCC
Cuts at: 1853 2101 1853
Size: 248 4173
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
SmaI CCC'GGG
Cuts at: 1443 1443
Size: 4421
SmlI C'TyrA_G
Cuts at: 246 1576 2491 2983 3245 246
Size: 1330 915 492 262 1422
Fragments arranged by size:
1422 1330 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4421
SphI G_CATG'C
Cuts at: 1474 1974 1474
Size: 500 3921
SspI AAT'ATT
Cuts at: 794 1056 794
Size: 262 4159
StyI C'CwwG_G
Cuts at: 276 1268 1555 1909 276
Size: 992 287 354 2788
Fragments arranged by size:
2788 992 354 287
SwaI ATTT'AAAT
Cuts at: 1112 1112
Size: 4421
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 563 1004 2004 2137 2596 2805 563
Size: 441 1000 133 459 209 2179
Fragments arranged by size:
2179 1000 459 441 209 133
TatI w'GTAC_w
Cuts at: 231 900 1059 3936 231
Size: 669 159 2877 716
Fragments arranged by size:
2877 716 669 159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3938 3970 3938
Size: 32 4389
Tth111I GACn'n_nGTC
Cuts at: 2218 2218
Size: 4421
XbaI T'CTAG_A
Cuts at: 1452 1452
Size: 4421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1916 1916
Size: 4421
XmaI C'CCGG_G
Cuts at: 1441 1441
Size: 4421
XmnI GAAnn'nnTTC
Cuts at: 863 913 863
Size: 50 4371
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BseRI BsiWI BsmBI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI XhoI ZraI