Restriction map for
pF1KB6619
(Circular) (Six-base) MAPSORT of: pF1KB6619.seq Check: 7629 from: 1 to: 4349
pF1KB6619 4349 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1146 1146
Size: 4349
AatII G_ACGT'C
Cuts at: 200 200
Size: 4349
AccI GT'mk_AC
Cuts at: 1072 1387 1072
Size: 315 4034
Acc65I G'GTAC_C
Cuts at: 1365 1365
Size: 4349
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 283 1324 2174 2606 3353 3621 199
Size: 84 1041 850 432 747 268 927
Fragments arranged by size:
1041 927 850 747 432 268 84
AflIII A'CryG_T
Cuts at: 1701 2805 3782 1701
Size: 1104 977 2268
Fragments arranged by size:
2268 1104 977
AgeI A'CCGG_T
Cuts at: 563 1075 2737 563
Size: 512 1662 2175
Fragments arranged by size:
2175 1662 512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1258 1258
Size: 4349
AlwNI CAG_nnn'CTG
Cuts at: 395 1654 3221 395
Size: 1259 1567 1523
Fragments arranged by size:
1567 1523 1259
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 553 1054 1102 1353 3702 3958 553
Size: 501 48 251 2349 256 944
Fragments arranged by size:
2349 944 501 256 251 48
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 775 941 1369 775
Size: 166 428 3755
Fragments arranged by size:
3755 428 166
BamHI G'GATC_C
Cuts at: 1374 1374
Size: 4349
BanI G'GyrC_C
Cuts at: 376 457 829 967 1365 1779 2027 2062 376
Size: 81 372 138 398 414 248 35 2663
Fragments arranged by size:
2663 414 398 372 248 138 81 35
BanII G_rGCy'C
Cuts at: 497 857 1363 2393 497
Size: 360 506 1030 2453
Fragments arranged by size:
2453 1030 506 360
BbeI G_GCGC'C
Cuts at: 461 1783 2031 461
Size: 1322 248 2779
Fragments arranged by size:
2779 1322 248
BbsI GAAGACnn'nnnn_
Cuts at: 239 739 239
Size: 500 3849
BciVI GTATCCnnnnn_n'
Cuts at: 2247 3008 4349 2247
Size: 761 1341 2247
Fragments arranged by size:
2247 1341 761
BclI T'GATC_A
Cuts at: 1869 1869
Size: 4349
BglI GCCn_nnn'nGGC
Cuts at: 211 211
Size: 4349
BglII A'GATC_T
Cuts at: 1864 3901 1864
Size: 2037 2312
BlpI GC'TnA_GC
Cuts at: 496 1061 1461 496
Size: 565 400 3384
Fragments arranged by size:
3384 565 400
Bme1580I G_kGCm'C
Cuts at: 379 1974 2067 3123 379
Size: 1595 93 1056 1605
Fragments arranged by size:
1605 1595 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1561 1642 1977 4121 1561
Size: 81 335 2144 1789
Fragments arranged by size:
2144 1789 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 4349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 561 701 712 1306 561
Size: 140 11 594 3604
Fragments arranged by size:
3604 594 140 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 1525 2404 2896 3194 1153
Size: 372 879 492 298 2308
Fragments arranged by size:
2308 879 492 372 298
BsaI GGTCTCn'nnnn_
Cuts at: 727 949 4053 727
Size: 222 3104 1023
Fragments arranged by size:
3104 1023 222
BsaAI yAC'GTr
Cuts at: 1702 1702
Size: 4349
BsaBI GATnn'nnATC
Cuts at: 6 616 1863 3900 6
Size: 610 1247 2037 455
Fragments arranged by size:
2037 1247 610 455
BsaHI Gr'CG_yC
Cuts at: 197 458 1780 2028 2730 4226 197
Size: 261 1322 248 702 1496 320
Fragments arranged by size:
1496 1322 702 320 261 248
BsaWI w'CCGG_w
Cuts at: 485 563 1075 1538 2059 2737 3011 3158 3569
Size: 78 512 463 521 678 274 147 411
Cuts at: 3569 485
Size: 1265
Fragments arranged by size:
1265 678 521 512 463 411 274 147
78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 829 859 829
Size: 30 4319
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 331 1237 331
Size: 906 3443
BseYI C'CCAG_C
Cuts at: 425 744 960 1774 2532 3109 3605 425
Size: 319 216 814 758 577 496 1169
Fragments arranged by size:
1169 814 758 577 496 319 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1174 1264 1174
Size: 90 4259
BsiEI CG_ry'CG
Cuts at: 104 1194 1891 1937 3145 104
Size: 1090 697 46 1208 1308
Fragments arranged by size:
1308 1208 1090 697 46
BsiHKAI G_wGCw'C
Cuts at: 497 857 1363 2141 2331 3123 497
Size: 360 506 778 190 792 1723
Fragments arranged by size:
1723 792 778 506 360 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1324 1324
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 379 497 857 1363 1974 2067 2141 2331 2393
Size: 118 360 506 611 93 74 190 62
Cuts at: 2393 3123 379
Size: 730 1605
Fragments arranged by size:
1605 730 611 506 360 190 118 93
74 62
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 3525 3525
Size: 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1146 1399 1613 1915 2296 1146
Size: 253 214 302 381 3199
Fragments arranged by size:
3199 381 302 253 214
BsrBI CCG'CTC
Cuts at: 50 2641 2695 4179 4344 50
Size: 2591 54 1484 165 55
Fragments arranged by size:
2591 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 200 530 585 2261 200
Size: 330 55 1676 2288
Fragments arranged by size:
2288 1676 330 55
BsrFI r'CCGG_y
Cuts at: 330 563 1075 2347 2528 2737 330
Size: 233 512 1272 181 209 1942
Fragments arranged by size:
1942 1272 512 233 209 181
BsrGI T'GTAC_A
Cuts at: 1181 1181
Size: 4349
BssSI C'ACGA_G
Cuts at: 159 169 2620 2978 159
Size: 10 2451 358 1530
Fragments arranged by size:
2451 1530 358 10
BstAPI GCAn_nnn'nTGC
Cuts at: 1726 1726
Size: 4349
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstEII G'GTnAC_C
Cuts at: 810 810
Size: 4349
BstYI r'GATC_y
Cuts at: 1 1374 1841 1864 2199 2445 3457 3543 3555
Size: 1373 467 23 335 246 1012 86 12
Cuts at: 3555 3901 1
Size: 346 449
Fragments arranged by size:
1373 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1073 1073
Size: 4349
Bsu36I CC'TnA_GG
Cuts at: 1297 1297
Size: 4349
BtgI C'CryG_G
Cuts at: 125 453 640 1010 125
Size: 328 187 370 3464
Fragments arranged by size:
3464 370 328 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 384 1104 2299 2480 2643 384
Size: 720 1195 181 163 2090
Fragments arranged by size:
2090 1195 720 181 163
BtsI GCAGTG_nn'
Cuts at: 1113 1334 1592 1113
Size: 221 258 3870
Fragments arranged by size:
3870 258 221
DraI TTT'AAA
Cuts at: 1348 1348
Size: 4349
DraIII CAC_nnn'GTG
Cuts at: 161 161
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 2055 2913 2055
Size: 858 3491
EaeI y'GGCC_r
Cuts at: 212 222 328 332 1191 1934 2108 2499 2526
Size: 10 106 4 859 743 174 391 27
Cuts at: 2526 3871 212
Size: 1345 690
Fragments arranged by size:
1345 859 743 690 391 174 106 27
10 4
EagI C'GGCC_G
Cuts at: 1191 1934 1191
Size: 743 3606
EarI CTCTTCn'nnn_
Cuts at: 862 2372 2582 862
Size: 1510 210 2629
Fragments arranged by size:
2629 1510 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1099 2557 2867 3013 4159 1099
Size: 1458 310 146 1146 1289
Fragments arranged by size:
1458 1289 1146 310 146
EcoICRI GAG'CTC
Cuts at: 495 855 1361 495
Size: 360 506 3483
Fragments arranged by size:
3483 506 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 283 561 701 712 1306 1324 2174 2606
Size: 84 278 140 11 594 18 850 432
Cuts at: 2606 3353 3621 199
Size: 747 268 927
Fragments arranged by size:
927 850 747 594 432 278 268 140
84 18 11
EcoO109I rG'GnC_Cy
Cuts at: 392 741 798 1488 392
Size: 349 57 690 3253
Fragments arranged by size:
3253 690 349 57
EcoRI G'AATT_C
Cuts at: 553 1353 553
Size: 800 3549
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 252 2368 252
Size: 2116 2233
FseI GG_CCGG'CC
Cuts at: 334 334
Size: 4349
FspI TGC'GCA
Cuts at: 1027 2130 1027
Size: 1103 3246
HaeII r_GCGC'y
Cuts at: 461 715 1783 2031 3053 4032 461
Size: 254 1068 248 1022 979 778
Fragments arranged by size:
1068 1022 979 778 254 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2191 2221 2223 2253 2191
Size: 30 2 30 4287
Fragments arranged by size:
4287 30 30 2
HincII GTy'rAC
Cuts at: 1388 1388
Size: 4349
HindIII A'AGCT_T
Cuts at: 265 265
Size: 4349
KasI G'GCGC_C
Cuts at: 457 1779 2027 457
Size: 1322 248 2779
Fragments arranged by size:
2779 1322 248
KpnI G_GTAC'C
Cuts at: 1369 1369
Size: 4349
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3020 3204 3020
Size: 184 4165
MscI TGG'CCA
Cuts at: 214 224 2110 214
Size: 10 1886 2453
Fragments arranged by size:
2453 1886 10
MslI CAynn'nnrTG
Cuts at: 169 931 1240 2338 2465 169
Size: 762 309 1098 127 2053
Fragments arranged by size:
2053 1098 762 309 127
MspA1I CmG'CkG
Cuts at: 429 500 1460 1774 2134 3147 3392 429
Size: 71 960 314 360 1013 245 1386
Fragments arranged by size:
1386 1013 960 360 314 245 71
NaeI GCC'GGC
Cuts at: 332 2530 332
Size: 2198 2151
NarI GG'CG_CC
Cuts at: 458 1780 2028 458
Size: 1322 248 2779
Fragments arranged by size:
2779 1322 248
NcoI C'CATG_G
Cuts at: 1010 1010
Size: 4349
NgoMIV G'CCGG_C
Cuts at: 330 2528 330
Size: 2198 2151
NspI r_CATG'y
Cuts at: 1402 1902 2809 1402
Size: 500 907 2942
Fragments arranged by size:
2942 907 500
PciI A'CATG_T
Cuts at: 2805 2805
Size: 4349
PfoI T'CCnGG_A
Cuts at: 689 689
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 769 801 3488 3520 769
Size: 32 2687 32 1598
Fragments arranged by size:
2687 1598 32 32
PpuMI rG'GwC_Cy
Cuts at: 741 798 741
Size: 57 4292
PshAI GACnn'nnGTC
Cuts at: 817 817
Size: 4349
PstI C_TGCA'G
Cuts at: 1396 2081 1396
Size: 685 3664
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 500 1774 2134 500
Size: 1274 360 2715
Fragments arranged by size:
2715 1274 360
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 497 857 1363 497
Size: 360 506 3483
Fragments arranged by size:
3483 506 360
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SanDI GG'GwC_CC
Cuts at: 798 798
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 862 2372 2582 862
Size: 1510 210 2629
Fragments arranged by size:
2629 1510 210
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 3866 3866
Size: 4349
SfcI C'TryA_G
Cuts at: 33 138 1392 2077 3070 3261 33
Size: 105 1254 685 993 191 1121
Fragments arranged by size:
1254 1121 993 685 191 105
SfoI GGC'GCC
Cuts at: 459 1781 2029 459
Size: 1322 248 2779
Fragments arranged by size:
2779 1322 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 1371 1371
Size: 4349
SmlI C'TyrA_G
Cuts at: 775 1168 1504 2419 2911 3173 775
Size: 393 336 915 492 262 1951
Fragments arranged by size:
1951 915 492 393 336 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
StuI AGG'CCT
Cuts at: 1003 1043 1003
Size: 40 4309
StyI C'CwwG_G
Cuts at: 848 1010 1483 1837 848
Size: 162 473 354 3360
Fragments arranged by size:
3360 473 354 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 850 1932 2065 2524 2733 850
Size: 1082 133 459 209 2466
Fragments arranged by size:
2466 1082 459 209 133
TatI w'GTAC_w
Cuts at: 1181 3864 1181
Size: 2683 1666
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 145 177 829 861 3866 3898 145
Size: 32 652 32 3005 32 596
Fragments arranged by size:
3005 652 596 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2146 2146
Size: 4349
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1844 1844
Size: 4349
XhoI C'TCGA_G
Cuts at: 775 775
Size: 4349
XmaI C'CCGG_G
Cuts at: 1369 1369
Size: 4349
XmnI GAAnn'nnTTC
Cuts at: 922 922
Size: 4349
ZraI GAC'GTC
Cuts at: 198 198
Size: 4349
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmI BssHII BstXI ClaI CspCI EcoNI EcoRV FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI