Restriction map for
pF1KB6612
(Circular) (Six-base) MAPSORT of: pF1KB6612.seq Check: 5478 from: 1 to: 4409
pF1KB6612 4409 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1447 1447
Size: 4409
Acc65I G'GTAC_C
Cuts at: 1425 1425
Size: 4409
AclI AA'CG_TT
Cuts at: 4249 4249
Size: 4409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 782 904 2234 2666 3413 3681 272
Size: 510 122 1330 432 747 268 1000
Fragments arranged by size:
1330 1000 747 510 432 268 122
AflIII A'CryG_T
Cuts at: 1761 2865 3842 1761
Size: 1104 977 2328
Fragments arranged by size:
2328 1104 977
AgeI A'CCGG_T
Cuts at: 2797 2797
Size: 4409
AlwNI CAG_nnn'CTG
Cuts at: 576 1261 1714 3281 576
Size: 685 453 1567 1704
Fragments arranged by size:
1704 1567 685 453
ApaI G_GGCC'C
Cuts at: 116 593 755 116
Size: 477 162 3770
Fragments arranged by size:
3770 477 162
ApaLI G'TGCA_C
Cuts at: 3179 3179
Size: 4409
ApoI r'AATT_y
Cuts at: 849 1413 3762 4018 849
Size: 564 2349 256 1240
Fragments arranged by size:
2349 1240 564 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4409
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
AvaI C'yCGr_G
Cuts at: 520 1010 1429 520
Size: 490 419 3500
Fragments arranged by size:
3500 490 419
BamHI G'GATC_C
Cuts at: 1434 1434
Size: 4409
BanI G'GyrC_C
Cuts at: 1425 1839 2087 2122 1425
Size: 414 248 35 3712
Fragments arranged by size:
3712 414 248 35
BanII G_rGCy'C
Cuts at: 116 593 746 755 1423 2453 116
Size: 477 153 9 668 1030 2072
Fragments arranged by size:
2072 1030 668 477 153 9
BbeI G_GCGC'C
Cuts at: 1843 2091 1843
Size: 248 4161
BbsI GAAGACnn'nnnn_
Cuts at: 1025 1025
Size: 4409
BciVI GTATCCnnnnn_n'
Cuts at: 528 850 2307 3068 4409 528
Size: 322 1457 761 1341 528
Fragments arranged by size:
1457 1341 761 528 322
BclI T'GATC_A
Cuts at: 1929 1929
Size: 4409
BglI GCCn_nnn'nGGC
Cuts at: 131 710 131
Size: 579 3830
BglII A'GATC_T
Cuts at: 1043 1924 3961 1043
Size: 881 2037 1491
Fragments arranged by size:
2037 1491 881
BlpI GC'TnA_GC
Cuts at: 1521 1521
Size: 4409
Bme1580I G_kGCm'C
Cuts at: 116 366 593 755 2034 2127 3183 116
Size: 250 227 162 1279 93 1056 1342
Fragments arranged by size:
1342 1279 1056 250 227 162 93
BmrI ACTGGGnnnn_n'
Cuts at: 1621 1702 2037 4181 1621
Size: 81 335 2144 1849
Fragments arranged by size:
2144 1849 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 4409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 715
Size: 4409
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 1585 2464 2956 3254 589
Size: 996 879 492 298 1744
Fragments arranged by size:
1744 996 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1339 4113 1339
Size: 2774 1635
BsaAI yAC'GTr
Cuts at: 1762 1762
Size: 4409
BsaBI GATnn'nnATC
Cuts at: 6 148 1923 3960 6
Size: 142 1775 2037 455
Fragments arranged by size:
2037 1775 455 142
BsaHI Gr'CG_yC
Cuts at: 1840 2088 2790 4286 1840
Size: 248 702 1496 1963
Fragments arranged by size:
1963 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1116 1598 2119 2797 3071 3218 3629 1116
Size: 482 521 678 274 147 411 1896
Fragments arranged by size:
1896 678 521 482 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 321 351 601 631 321
Size: 30 250 30 4099
Fragments arranged by size:
4099 250 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 606 883 1155 1264 606
Size: 277 272 109 3751
Fragments arranged by size:
3751 277 272 109
BseYI C'CCAG_C
Cuts at: 129 576 1243 1834 2592 3169 3665 129
Size: 447 667 591 758 577 496 873
Fragments arranged by size:
873 758 667 591 577 496 447
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 671
Size: 4409
BsiEI CG_ry'CG
Cuts at: 104 1951 1997 3205 104
Size: 1847 46 1208 1308
Fragments arranged by size:
1847 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 220 605 746 1423 2201 2391 3183 220
Size: 385 141 677 778 190 792 1446
Fragments arranged by size:
1446 792 778 677 385 190 141
Bsp1286I G_dGCh'C
Cuts at: 116 220 366 593 605 746 755 1423 2034
Size: 104 146 227 12 141 9 668 611
Cuts at: 2034 2127 2201 2391 2453 3183 116
Size: 93 74 190 62 730 1342
Fragments arranged by size:
1342 730 668 611 227 190 146 141
104 93 74 62 12 9
BspEI T'CCGG_A
Cuts at: 1116 3629 1116
Size: 2513 1896
BspHI T'CATG_A
Cuts at: 3585 3585
Size: 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 415 1459 1673 1975 2356 415
Size: 1044 214 302 381 2468
Fragments arranged by size:
2468 1044 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2701 2755 4239 4404 50
Size: 2651 54 1484 165 55
Fragments arranged by size:
2651 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2321 2321
Size: 4409
BsrFI r'CCGG_y
Cuts at: 2407 2588 2797 2407
Size: 181 209 4019
Fragments arranged by size:
4019 209 181
BssSI C'ACGA_G
Cuts at: 2680 3038 2680
Size: 358 4051
BstAPI GCAn_nnn'nTGC
Cuts at: 334 1786 334
Size: 1452 2957
BstBI TT'CG_AA
Cuts at: 1202 2770 1202
Size: 1568 2841
BstEII G'GTnAC_C
Cuts at: 1157 1157
Size: 4409
BstYI r'GATC_y
Cuts at: 1 447 1043 1434 1901 1924 2259 2505 3517
Size: 446 596 391 467 23 335 246 1012
Cuts at: 3517 3603 3615 3961 1
Size: 86 12 346 449
Fragments arranged by size:
1012 596 467 449 446 391 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 116 1329 107
Size: 9 1213 3187
Fragments arranged by size:
3187 1213 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2359 2540 2703 2359
Size: 181 163 4065
Fragments arranged by size:
4065 181 163
BtsI GCAGTG_nn'
Cuts at: 354 536 1652 354
Size: 182 1116 3111
Fragments arranged by size:
3111 1116 182
DraI TTT'AAA
Cuts at: 1408 1408
Size: 4409
DraIII CAC_nnn'GTG
Cuts at: 158 158
Size: 4409
DrdI GACnn_nn'nnGTC
Cuts at: 2115 2973 2115
Size: 858 3551
EaeI y'GGCC_r
Cuts at: 1323 1994 2168 2559 2586 3931 1323
Size: 671 174 391 27 1345 1801
Fragments arranged by size:
1801 1345 671 391 174 27
EagI C'GGCC_G
Cuts at: 1994 1994
Size: 4409
EarI CTCTTCn'nnn_
Cuts at: 860 941 1034 2432 2642 860
Size: 81 93 1398 210 2627
Fragments arranged by size:
2627 1398 210 93 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 958 2617 2927 3073 4219 958
Size: 1659 310 146 1146 1148
Fragments arranged by size:
1659 1148 1146 310 146
EcoICRI GAG'CTC
Cuts at: 744 1421 744
Size: 677 3732
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 715 782 904 2234 2666 3413 3681 272
Size: 443 67 122 1330 432 747 268 1000
Fragments arranged by size:
1330 1000 747 443 432 268 122 67
EcoO109I rG'GnC_Cy
Cuts at: 112 589 590 751 752 873 1146 1548 112
Size: 477 1 161 1 121 273 402 2973
Fragments arranged by size:
2973 477 402 273 161 121 1 1
EcoRI G'AATT_C
Cuts at: 1413 1413
Size: 4409
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4409
FspI TGC'GCA
Cuts at: 2190 2190
Size: 4409
HaeII r_GCGC'y
Cuts at: 1843 2091 3113 4092 1843
Size: 248 1022 979 2160
Fragments arranged by size:
2160 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 601 633 1266 1298 2251 2281 2283 2313 601
Size: 32 633 32 953 30 2 30 2697
Fragments arranged by size:
2697 953 633 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1448 1448
Size: 4409
HindIII A'AGCT_T
Cuts at: 895 895
Size: 4409
KasI G'GCGC_C
Cuts at: 1839 2087 1839
Size: 248 4161
KpnI G_GTAC'C
Cuts at: 1429 1429
Size: 4409
MluI A'CGCG_T
Cuts at: 3842 3842
Size: 4409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3080 3264 3080
Size: 184 4225
MscI TGG'CCA
Cuts at: 1325 2170 1325
Size: 845 3564
MslI CAynn'nnrTG
Cuts at: 2398 2525 2398
Size: 127 4282
MspA1I CmG'CkG
Cuts at: 118 137 685 1520 1834 2194 3207 3452 118
Size: 19 548 835 314 360 1013 245 1075
Fragments arranged by size:
1075 1013 835 548 360 314 245 19
NaeI GCC'GGC
Cuts at: 2590 2590
Size: 4409
NarI GG'CG_CC
Cuts at: 1840 2088 1840
Size: 248 4161
NcoI C'CATG_G
Cuts at: 107 107
Size: 4409
NgoMIV G'CCGG_C
Cuts at: 2588 2588
Size: 4409
NspI r_CATG'y
Cuts at: 414 1462 1962 2869 414
Size: 1048 500 907 1954
Fragments arranged by size:
1954 1048 907 500
PasI CC'CwG_GG
Cuts at: 748 1311 748
Size: 563 3846
PciI A'CATG_T
Cuts at: 2865 2865
Size: 4409
PflMI CCAn_nnn'nTGG
Cuts at: 576 576
Size: 4409
PmeI GTTT'AAAC
Cuts at: 1408 1408
Size: 4409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 3548
Size: 32 4377
PpuMI rG'GwC_Cy
Cuts at: 1146 1146
Size: 4409
PspOMI G'GGCC_C
Cuts at: 112 589 751 112
Size: 477 162 3770
Fragments arranged by size:
3770 477 162
PstI C_TGCA'G
Cuts at: 684 1456 2141 684
Size: 772 685 2952
Fragments arranged by size:
2952 772 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4409
PvuII CAG'CTG
Cuts at: 685 1834 2194 685
Size: 1149 360 2900
Fragments arranged by size:
2900 1149 360
RsrII CG'GwC_CG
Cuts at: 2604 2604
Size: 4409
SacI G_AGCT'C
Cuts at: 746 1423 746
Size: 677 3732
SacII CC_GC'GG
Cuts at: 119 119
Size: 4409
SalI G'TCGA_C
Cuts at: 1446 1446
Size: 4409
SanDI GG'GwC_CC
Cuts at: 1146 1146
Size: 4409
SapI GCTCTTCn'nnn_
Cuts at: 2432 2642 2432
Size: 210 4199
SbfI CC_TGCA'GG
Cuts at: 1456 1456
Size: 4409
ScaI AGT'ACT
Cuts at: 399 3926 399
Size: 3527 882
SfcI C'TryA_G
Cuts at: 33 680 1452 2137 3130 3321 33
Size: 647 772 685 993 191 1121
Fragments arranged by size:
1121 993 772 685 647 191
SfoI GGC'GCC
Cuts at: 1841 2089 1841
Size: 248 4161
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
SmaI CCC'GGG
Cuts at: 1431 1431
Size: 4409
SmlI C'TyrA_G
Cuts at: 604 1564 2479 2971 3233 604
Size: 960 915 492 262 1780
Fragments arranged by size:
1780 960 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4409
SphI G_CATG'C
Cuts at: 414 1462 1962 414
Size: 1048 500 2861
Fragments arranged by size:
2861 1048 500
StyI C'CwwG_G
Cuts at: 107 843 1543 1897 107
Size: 736 700 354 2619
Fragments arranged by size:
2619 736 700 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1992 2125 2584 2793 1992
Size: 133 459 209 3608
Fragments arranged by size:
3608 459 209 133
TatI w'GTAC_w
Cuts at: 397 1055 3924 397
Size: 658 2869 882
Fragments arranged by size:
2869 882 658
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 869 901 1070 1102 3926 3958 869
Size: 32 169 32 2824 32 1320
Fragments arranged by size:
2824 1320 169 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2206 2206
Size: 4409
XbaI T'CTAG_A
Cuts at: 1440 1440
Size: 4409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1904 1904
Size: 4409
XmaI C'CCGG_G
Cuts at: 1429 1429
Size: 4409
XmnI GAAnn'nnTTC
Cuts at: 917 917
Size: 4409
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PsiI PspXI PsrI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI