Restriction map for
pF1KB6608
(Circular) (Six-base) MAPSORT of: pF1KB6608.seq Check: 6786 from: 1 to: 4406
pF1KB6608 4406 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 504 956 504
Size: 452 3954
AccI GT'mk_AC
Cuts at: 818 1331 1444 818
Size: 513 113 3780
Fragments arranged by size:
3780 513 113
Acc65I G'GTAC_C
Cuts at: 1422 1422
Size: 4406
AclI AA'CG_TT
Cuts at: 4246 4246
Size: 4406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 427 964 1187 1333 2231 2663 3410 3678
Size: 36 537 223 146 898 432 747 268
Cuts at: 3678 391
Size: 1119
Fragments arranged by size:
1119 898 747 537 432 268 223 146
36
AfeI AGC'GCT
Cuts at: 906 906
Size: 4406
AflIII A'CryG_T
Cuts at: 220 728 1758 2862 3839 220
Size: 508 1030 1104 977 787
Fragments arranged by size:
1104 1030 977 787 508
AgeI A'CCGG_T
Cuts at: 2794 2794
Size: 4406
AhdI GACnn_n'nnGTC
Cuts at: 1246 1246
Size: 4406
AleI CACnn'nnGTG
Cuts at: 727 727
Size: 4406
AlwNI CAG_nnn'CTG
Cuts at: 1711 3278 1711
Size: 1567 2839
ApaLI G'TGCA_C
Cuts at: 3176 3176
Size: 4406
ApoI r'AATT_y
Cuts at: 274 601 655 1071 1410 3759 4015 274
Size: 327 54 416 339 2349 256 665
Fragments arranged by size:
2349 665 416 339 327 256 54
AscI GG'CGCG_CC
Cuts at: 124 124
Size: 4406
AseI AT'TA_AT
Cuts at: 20 20
Size: 4406
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
AvaI C'yCGr_G
Cuts at: 1426 1426
Size: 4406
BamHI G'GATC_C
Cuts at: 1361 1431 1361
Size: 70 4336
BanI G'GyrC_C
Cuts at: 704 1422 1836 2084 2119 704
Size: 718 414 248 35 2991
Fragments arranged by size:
2991 718 414 248 35
BanII G_rGCy'C
Cuts at: 117 718 1420 2450 117
Size: 601 702 1030 2073
Fragments arranged by size:
2073 1030 702 601
BbeI G_GCGC'C
Cuts at: 1840 2088 1840
Size: 248 4158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 485 519 485
Size: 34 4372
BciVI GTATCCnnnnn_n'
Cuts at: 2304 3065 4406 2304
Size: 761 1341 2304
Fragments arranged by size:
2304 1341 761
BclI T'GATC_A
Cuts at: 764 1926 764
Size: 1162 3244
BglI GCCn_nnn'nGGC
Cuts at: 121 121
Size: 4406
BglII A'GATC_T
Cuts at: 430 1921 3958 430
Size: 1491 2037 878
Fragments arranged by size:
2037 1491 878
BlpI GC'TnA_GC
Cuts at: 1518 1518
Size: 4406
Bme1580I G_kGCm'C
Cuts at: 1311 2031 2124 3180 1311
Size: 720 93 1056 2537
Fragments arranged by size:
2537 1056 720 93
BmrI ACTGGGnnnn_n'
Cuts at: 1023 1618 1699 2034 4178 1023
Size: 595 81 335 2144 1251
Fragments arranged by size:
2144 1251 595 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 760 2773 760
Size: 2013 2393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 572
Size: 4406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 816 1582 2461 2953 3251 816
Size: 766 879 492 298 1971
Fragments arranged by size:
1971 879 766 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4110 4110
Size: 4406
BsaAI yAC'GTr
Cuts at: 729 1759 729
Size: 1030 3376
BsaBI GATnn'nnATC
Cuts at: 6 1920 3957 6
Size: 1914 2037 455
Fragments arranged by size:
2037 1914 455
BsaHI Gr'CG_yC
Cuts at: 1328 1837 2085 2787 4283 1328
Size: 509 248 702 1496 1451
Fragments arranged by size:
1496 1451 702 509 248
BsaWI w'CCGG_w
Cuts at: 488 1092 1595 2116 2794 3068 3215 3626 488
Size: 604 503 521 678 274 147 411 1268
Fragments arranged by size:
1268 678 604 521 503 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 850 880 952 982 850
Size: 30 72 30 4274
Fragments arranged by size:
4274 72 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 570 575 570
Size: 5 4401
BseYI C'CCAG_C
Cuts at: 447 758 1292 1831 2589 3166 3662 447
Size: 311 534 539 758 577 496 1191
Fragments arranged by size:
1191 758 577 539 534 496 311
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 532
Size: 4406
BsiEI CG_ry'CG
Cuts at: 104 1948 1994 3202 104
Size: 1844 46 1208 1308
Fragments arranged by size:
1844 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1420 2198 2388 3180 1420
Size: 778 190 792 2646
Fragments arranged by size:
2646 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 117 718 1311 1420 2031 2124 2198 2388 2450
Size: 601 593 109 611 93 74 190 62
Cuts at: 2450 3180 117
Size: 730 1343
Fragments arranged by size:
1343 730 611 601 593 190 109 93
74 62
BspEI T'CCGG_A
Cuts at: 1092 3626 1092
Size: 2534 1872
BspHI T'CATG_A
Cuts at: 3582 3582
Size: 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 322 504 956 1456 1670 1972 2353 322
Size: 182 452 500 214 302 381 2375
Fragments arranged by size:
2375 500 452 381 302 214 182
BsrBI CCG'CTC
Cuts at: 50 2698 2752 4236 4401 50
Size: 2648 54 1484 165 55
Fragments arranged by size:
2648 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 177 932 2318 177
Size: 755 1386 2265
Fragments arranged by size:
2265 1386 755
BsrFI r'CCGG_y
Cuts at: 371 2404 2585 2794 371
Size: 2033 181 209 1983
Fragments arranged by size:
2033 1983 209 181
BssHII G'CGCG_C
Cuts at: 124 124
Size: 4406
BssSI C'ACGA_G
Cuts at: 1335 2677 3035 1335
Size: 1342 358 2706
Fragments arranged by size:
2706 1342 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1783 1783
Size: 4406
BstBI TT'CG_AA
Cuts at: 2767 2767
Size: 4406
BstYI r'GATC_y
Cuts at: 1 430 947 1361 1431 1898 1921 2256 2502
Size: 429 517 414 70 467 23 335 246
Cuts at: 2502 3514 3600 3612 3958 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 517 467 449 429 414 346 335
246 86 70 23 12
BtgI C'CryG_G
Cuts at: 365 1299 365
Size: 934 3472
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2356 2537 2700 2356
Size: 181 163 4062
Fragments arranged by size:
4062 181 163
BtsI GCAGTG_nn'
Cuts at: 777 1649 777
Size: 872 3534
DraI TTT'AAA
Cuts at: 1405 1405
Size: 4406
DrdI GACnn_nn'nnGTC
Cuts at: 814 2112 2970 814
Size: 1298 858 2250
Fragments arranged by size:
2250 1298 858
EaeI y'GGCC_r
Cuts at: 612 689 1991 2165 2556 2583 3928 612
Size: 77 1302 174 391 27 1345 1090
Fragments arranged by size:
1345 1302 1090 391 174 77 27
EagI C'GGCC_G
Cuts at: 1991 1991
Size: 4406
EarI CTCTTCn'nnn_
Cuts at: 446 1376 2429 2639 446
Size: 930 1053 210 2213
Fragments arranged by size:
2213 1053 930 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2614 2924 3070 4216 2614
Size: 310 146 1146 2804
Fragments arranged by size:
2804 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1418 1418
Size: 4406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 427 572 964 1187 1333 2231 2663 3410
Size: 36 145 392 223 146 898 432 747
Cuts at: 3410 3678 391
Size: 268 1119
Fragments arranged by size:
1119 898 747 432 392 268 223 146
145 36
EcoNI CCTnn'n_nnAGG
Cuts at: 892 892
Size: 4406
EcoO109I rG'GnC_Cy
Cuts at: 629 1240 1545 629
Size: 611 305 3490
Fragments arranged by size:
3490 611 305
EcoRI G'AATT_C
Cuts at: 655 1071 1410 655
Size: 416 339 3651
Fragments arranged by size:
3651 416 339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4406
FseI GG_CCGG'CC
Cuts at: 375 375
Size: 4406
FspI TGC'GCA
Cuts at: 561 974 2187 561
Size: 413 1213 2780
Fragments arranged by size:
2780 1213 413
HaeII r_GCGC'y
Cuts at: 773 908 1840 2088 3110 4089 773
Size: 135 932 248 1022 979 1090
Fragments arranged by size:
1090 1022 979 932 248 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1232 1264 1353 1385 2248 2278 2280 2310 1232
Size: 32 89 32 863 30 2 30 3328
Fragments arranged by size:
3328 863 89 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1445 1445
Size: 4406
KasI G'GCGC_C
Cuts at: 1836 2084 1836
Size: 248 4158
KpnI G_GTAC'C
Cuts at: 1426 1426
Size: 4406
MluI A'CGCG_T
Cuts at: 3839 3839
Size: 4406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 254 269 869 3077 3261 254
Size: 15 600 2208 184 1399
Fragments arranged by size:
2208 1399 600 184 15
MscI TGG'CCA
Cuts at: 614 2167 614
Size: 1553 2853
MslI CAynn'nnrTG
Cuts at: 727 1180 2395 2522 727
Size: 453 1215 127 2611
Fragments arranged by size:
2611 1215 453 127
MspA1I CmG'CkG
Cuts at: 447 1101 1517 1831 2191 3204 3449 447
Size: 654 416 314 360 1013 245 1404
Fragments arranged by size:
1404 1013 654 416 360 314 245
NaeI GCC'GGC
Cuts at: 373 2587 373
Size: 2214 2192
NarI GG'CG_CC
Cuts at: 1837 2085 1837
Size: 248 4158
NcoI C'CATG_G
Cuts at: 365 365
Size: 4406
NgoMIV G'CCGG_C
Cuts at: 371 2585 371
Size: 2214 2192
NspI r_CATG'y
Cuts at: 224 1459 1959 2866 224
Size: 1235 500 907 1764
Fragments arranged by size:
1764 1235 907 500
PciI A'CATG_T
Cuts at: 220 2862 220
Size: 2642 1764
PfoI T'CCnGG_A
Cuts at: 982 982
Size: 4406
PmeI GTTT'AAAC
Cuts at: 1405 1405
Size: 4406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 4374
PpuMI rG'GwC_Cy
Cuts at: 1240 1240
Size: 4406
PstI C_TGCA'G
Cuts at: 475 1453 2138 475
Size: 978 685 2743
Fragments arranged by size:
2743 978 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4406
PvuII CAG'CTG
Cuts at: 447 1101 1831 2191 447
Size: 654 730 360 2662
Fragments arranged by size:
2662 730 654 360
RsrII CG'GwC_CG
Cuts at: 2601 2601
Size: 4406
SacI G_AGCT'C
Cuts at: 1420 1420
Size: 4406
SalI G'TCGA_C
Cuts at: 1443 1443
Size: 4406
SanDI GG'GwC_CC
Cuts at: 1240 1240
Size: 4406
SapI GCTCTTCn'nnn_
Cuts at: 446 2429 2639 446
Size: 1983 210 2213
Fragments arranged by size:
2213 1983 210
SbfI CC_TGCA'GG
Cuts at: 1453 1453
Size: 4406
ScaI AGT'ACT
Cuts at: 238 3923 238
Size: 3685 721
SfcI C'TryA_G
Cuts at: 33 471 696 900 1449 2134 3127 3318 33
Size: 438 225 204 549 685 993 191 1121
Fragments arranged by size:
1121 993 685 549 438 225 204 191
SfoI GGC'GCC
Cuts at: 1838 2086 1838
Size: 248 4158
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4406
SmaI CCC'GGG
Cuts at: 1428 1428
Size: 4406
SmlI C'TyrA_G
Cuts at: 831 1561 2476 2968 3230 831
Size: 730 915 492 262 2007
Fragments arranged by size:
2007 915 730 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4406
SphI G_CATG'C
Cuts at: 1459 1959 1459
Size: 500 3906
StyI C'CwwG_G
Cuts at: 365 956 1260 1376 1540 1894 365
Size: 591 304 116 164 354 2877
Fragments arranged by size:
2877 591 354 304 164 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 750 1989 2122 2581 2790 750
Size: 1239 133 459 209 2366
Fragments arranged by size:
2366 1239 459 209 133
TatI w'GTAC_w
Cuts at: 236 3921 236
Size: 3685 721
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 209 241 952 984 3923 3955 209
Size: 32 711 32 2939 32 660
Fragments arranged by size:
2939 711 660 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2203 2203
Size: 4406
XbaI T'CTAG_A
Cuts at: 1437 1437
Size: 4406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1901 1901
Size: 4406
XmaI C'CCGG_G
Cuts at: 1426 1426
Size: 4406
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AlfI AloI ApaI AvrII BaeI BbsI
BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI
BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI