Restriction map for
pF1KB6593
(Circular) (Six-base) MAPSORT of: pF1KB6593.seq Check: 4594 from: 1 to: 4244
pF1KB6593 4244 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1282 1282
Size: 4244
Acc65I G'GTAC_C
Cuts at: 1260 1260
Size: 4244
AclI AA'CG_TT
Cuts at: 4084 4084
Size: 4244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 887 910 1096 2069 2501 3248 3516 418
Size: 469 23 186 973 432 747 268 1146
Fragments arranged by size:
1146 973 747 469 432 268 186 23
AflIII A'CryG_T
Cuts at: 581 1596 2700 3677 581
Size: 1015 1104 977 1148
Fragments arranged by size:
1148 1104 1015 977
AgeI A'CCGG_T
Cuts at: 604 2632 604
Size: 2028 2216
AleI CACnn'nnGTG
Cuts at: 127 127
Size: 4244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1090 1122 1090
Size: 32 4212
AlwNI CAG_nnn'CTG
Cuts at: 310 832 1549 3116 310
Size: 522 717 1567 1438
Fragments arranged by size:
1567 1438 717 522
ApaI G_GGCC'C
Cuts at: 638 638
Size: 4244
ApaLI G'TGCA_C
Cuts at: 3014 3014
Size: 4244
ApoI r'AATT_y
Cuts at: 721 1248 3597 3853 721
Size: 527 2349 256 1112
Fragments arranged by size:
2349 1112 527 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 1102 1264 1102
Size: 162 4082
BamHI G'GATC_C
Cuts at: 1143 1269 1143
Size: 126 4118
BanI G'GyrC_C
Cuts at: 688 706 1260 1674 1922 1957 688
Size: 18 554 414 248 35 2975
Fragments arranged by size:
2975 554 414 248 35 18
BanII G_rGCy'C
Cuts at: 638 1180 1258 2288 638
Size: 542 78 1030 2594
Fragments arranged by size:
2594 1030 542 78
BbeI G_GCGC'C
Cuts at: 1678 1926 1678
Size: 248 3996
BbsI GAAGACnn'nnnn_
Cuts at: 875 1235 875
Size: 360 3884
BbvCI CC'TCA_GC
Cuts at: 839 839
Size: 4244
BciVI GTATCCnnnnn_n'
Cuts at: 328 2142 2903 4244 328
Size: 1814 761 1341 328
Fragments arranged by size:
1814 1341 761 328
BclI T'GATC_A
Cuts at: 1764 1764
Size: 4244
BglI GCCn_nnn'nGGC
Cuts at: 391 633 817 1192 391
Size: 242 184 375 3443
Fragments arranged by size:
3443 375 242 184
BglII A'GATC_T
Cuts at: 1759 3796 1759
Size: 2037 2207
BlpI GC'TnA_GC
Cuts at: 1041 1356 1041
Size: 315 3929
Bme1580I G_kGCm'C
Cuts at: 638 1869 1962 3018 638
Size: 1231 93 1056 1864
Fragments arranged by size:
1864 1231 1056 93
BmgBI CAC'GTC
Cuts at: 1089 1089
Size: 4244
BmrI ACTGGGnnnn_n'
Cuts at: 448 632 731 869 935 1456 1537 1872 4016
Size: 184 99 138 66 521 81 335 2144
Cuts at: 4016 448
Size: 676
Fragments arranged by size:
2144 676 521 335 184 138 99 81
66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4244
Bpu10I CC'TnA_GC
Cuts at: 749 839 940 749
Size: 90 101 4053
Fragments arranged by size:
4053 101 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 1420 2299 2791 3089 484
Size: 936 879 492 298 1639
Fragments arranged by size:
1639 936 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3948 3948
Size: 4244
BsaAI yAC'GTr
Cuts at: 1597 1597
Size: 4244
BsaBI GATnn'nnATC
Cuts at: 6 430 898 1758 3795 6
Size: 424 468 860 2037 455
Fragments arranged by size:
2037 860 468 455 424
BsaHI Gr'CG_yC
Cuts at: 1675 1923 2625 4121 1675
Size: 248 702 1496 1798
Fragments arranged by size:
1798 1496 702 248
BsaWI w'CCGG_w
Cuts at: 604 1433 1954 2632 2906 3053 3464 604
Size: 829 521 678 274 147 411 1384
Fragments arranged by size:
1384 829 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 612 642 612
Size: 30 4214
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 559 817 820 1087 559
Size: 258 3 267 3716
Fragments arranged by size:
3716 267 258 3
BseYI C'CCAG_C
Cuts at: 191 696 1669 2427 3004 3500 191
Size: 505 973 758 577 496 935
Fragments arranged by size:
973 935 758 577 505 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 351
Size: 4244
BsiEI CG_ry'CG
Cuts at: 104 1786 1832 3040 104
Size: 1682 46 1208 1308
Fragments arranged by size:
1682 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1258 2036 2226 3018 1258
Size: 778 190 792 2484
Fragments arranged by size:
2484 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 638 1180 1258 1869 1962 2036 2226 2288 3018
Size: 542 78 611 93 74 190 62 730
Cuts at: 3018 638
Size: 1864
Fragments arranged by size:
1864 730 611 542 190 93 78 74
62
BspEI T'CCGG_A
Cuts at: 3464 3464
Size: 4244
BspHI T'CATG_A
Cuts at: 3420 3420
Size: 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1294 1508 1810 2191 1294
Size: 214 302 381 3347
Fragments arranged by size:
3347 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2536 2590 4074 4239 50
Size: 2486 54 1484 165 55
Fragments arranged by size:
2486 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 434 2156 434
Size: 1722 2522
BsrFI r'CCGG_y
Cuts at: 604 2242 2423 2632 604
Size: 1638 181 209 2216
Fragments arranged by size:
2216 1638 209 181
BssSI C'ACGA_G
Cuts at: 2515 2873 2515
Size: 358 3886
BstAPI GCAn_nnn'nTGC
Cuts at: 832 1621 832
Size: 789 3455
BstBI TT'CG_AA
Cuts at: 1164 2605 1164
Size: 1441 2803
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4244
BstYI r'GATC_y
Cuts at: 1 474 732 1143 1269 1736 1759 2094 2340
Size: 473 258 411 126 467 23 335 246
Cuts at: 2340 3352 3438 3450 3796 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 473 467 449 411 346 335 258
246 126 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2194 2375 2538 2194
Size: 181 163 3900
Fragments arranged by size:
3900 181 163
BtsI GCAGTG_nn'
Cuts at: 1487 1487
Size: 4244
DraI TTT'AAA
Cuts at: 1243 1243
Size: 4244
DrdI GACnn_nn'nnGTC
Cuts at: 1950 2808 1950
Size: 858 3386
EaeI y'GGCC_r
Cuts at: 312 918 1008 1829 2003 2394 2421 3766 312
Size: 606 90 821 174 391 27 1345 790
Fragments arranged by size:
1345 821 790 606 391 174 90 27
EagI C'GGCC_G
Cuts at: 1829 1829
Size: 4244
EarI CTCTTCn'nnn_
Cuts at: 536 776 953 2267 2477 536
Size: 240 177 1314 210 2303
Fragments arranged by size:
2303 1314 240 210 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1030 2452 2762 2908 4054 1030
Size: 1422 310 146 1146 1220
Fragments arranged by size:
1422 1220 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1256 1256
Size: 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 887 910 1096 2069 2501 3248 3516 418
Size: 469 23 186 973 432 747 268 1146
Fragments arranged by size:
1146 973 747 469 432 268 186 23
EcoNI CCTnn'n_nnAGG
Cuts at: 502 502
Size: 4244
EcoO109I rG'GnC_Cy
Cuts at: 1098 1383 1098
Size: 285 3959
EcoRI G'AATT_C
Cuts at: 1248 1248
Size: 4244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 378 2263 378
Size: 1885 2359
FspI TGC'GCA
Cuts at: 430 2025 430
Size: 1595 2649
FspAI rTGC'GCAy
Cuts at: 430 430
Size: 4244
HaeII r_GCGC'y
Cuts at: 1678 1926 2948 3927 1678
Size: 248 1022 979 1995
Fragments arranged by size:
1995 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 612 644 1024 1056 2086 2116 2118 2148 612
Size: 32 380 32 1030 30 2 30 2708
Fragments arranged by size:
2708 1030 380 32 32 30 30 2
HincII GTy'rAC
Cuts at: 207 522 1283 207
Size: 315 761 3168
Fragments arranged by size:
3168 761 315
KasI G'GCGC_C
Cuts at: 1674 1922 1674
Size: 248 3996
KpnI G_GTAC'C
Cuts at: 1264 1264
Size: 4244
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 95 366 2915 3099 95
Size: 271 2549 184 1240
Fragments arranged by size:
2549 1240 271 184
MscI TGG'CCA
Cuts at: 1010 2005 1010
Size: 995 3249
MslI CAynn'nnrTG
Cuts at: 127 379 982 2233 2360 127
Size: 252 603 1251 127 2011
Fragments arranged by size:
2011 1251 603 252 127
MspA1I CmG'CkG
Cuts at: 789 795 823 1355 1669 2029 3042 3287 789
Size: 6 28 532 314 360 1013 245 1746
Fragments arranged by size:
1746 1013 532 360 314 245 28 6
NaeI GCC'GGC
Cuts at: 2425 2425
Size: 4244
NarI GG'CG_CC
Cuts at: 1675 1923 1675
Size: 248 3996
NcoI C'CATG_G
Cuts at: 107 107
Size: 4244
NgoMIV G'CCGG_C
Cuts at: 2423 2423
Size: 4244
NspI r_CATG'y
Cuts at: 1297 1797 2704 1297
Size: 500 907 2837
Fragments arranged by size:
2837 907 500
PciI A'CATG_T
Cuts at: 2700 2700
Size: 4244
PflMI CCAn_nnn'nTGG
Cuts at: 310 310
Size: 4244
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 594 626 3383 3415 594
Size: 32 2757 32 1423
Fragments arranged by size:
2757 1423 32 32
PpuMI rG'GwC_Cy
Cuts at: 1098 1098
Size: 4244
PspOMI G'GGCC_C
Cuts at: 634 634
Size: 4244
PstI C_TGCA'G
Cuts at: 188 828 995 1291 1976 188
Size: 640 167 296 685 2456
Fragments arranged by size:
2456 685 640 296 167
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 823 1669 2029 823
Size: 846 360 3038
Fragments arranged by size:
3038 846 360
RsrII CG'GwC_CG
Cuts at: 2439 2439
Size: 4244
SacI G_AGCT'C
Cuts at: 1258 1258
Size: 4244
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 776 953 2267 2477 776
Size: 177 1314 210 2543
Fragments arranged by size:
2543 1314 210 177
SbfI CC_TGCA'GG
Cuts at: 995 1291 995
Size: 296 3948
ScaI AGT'ACT
Cuts at: 3761 3761
Size: 4244
SfcI C'TryA_G
Cuts at: 33 184 824 991 1287 1972 2965 3156 33
Size: 151 640 167 296 685 993 191 1121
Fragments arranged by size:
1121 993 685 640 296 191 167 151
SfiI GGCCn_nnn'nGGCC
Cuts at: 1192 1192
Size: 4244
SfoI GGC'GCC
Cuts at: 1676 1924 1676
Size: 248 3996
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SmaI CCC'GGG
Cuts at: 1266 1266
Size: 4244
SmlI C'TyrA_G
Cuts at: 499 1399 2314 2806 3068 499
Size: 900 915 492 262 1675
Fragments arranged by size:
1675 915 900 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 1297 1797 1297
Size: 500 3744
StyI C'CwwG_G
Cuts at: 107 347 758 1067 1199 1378 1732 107
Size: 240 411 309 132 179 354 2619
Fragments arranged by size:
2619 411 354 309 240 179 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1827 1960 2419 2628 1827
Size: 133 459 209 3443
Fragments arranged by size:
3443 459 209 133
TatI w'GTAC_w
Cuts at: 3759 3759
Size: 4244
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 440 472 915 947 3761 3793 440
Size: 32 443 32 2814 32 891
Fragments arranged by size:
2814 891 443 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2041 2041
Size: 4244
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 913 1044 1739 913
Size: 131 695 3418
Fragments arranged by size:
3418 695 131
XmaI C'CCGG_G
Cuts at: 1264 1264
Size: 4244
XmnI GAAnn'nnTTC
Cuts at: 606 1160 606
Size: 554 3690
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AscI AvrII
BaeI BcgI BfrBI BmtI BpmI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI