Restriction map for
pF1KB6591
(Circular) (Six-base) MAPSORT of: pF1KB6591.seq Check: 90 from: 1 to: 4253
pF1KB6591 4253 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AatII G_ACGT'C
Cuts at: 722 722
Size: 4253
AccI GT'mk_AC
Cuts at: 302 461 1291 302
Size: 159 830 3264
Fragments arranged by size:
3264 830 159
Acc65I G'GTAC_C
Cuts at: 1269 1269
Size: 4253
AclI AA'CG_TT
Cuts at: 4093 4093
Size: 4253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 2078 2510 3257 3525 1171
Size: 907 432 747 268 1899
Fragments arranged by size:
1899 907 747 432 268
AflIII A'CryG_T
Cuts at: 530 1605 2709 3686 530
Size: 1075 1104 977 1097
Fragments arranged by size:
1104 1097 1075 977
AgeI A'CCGG_T
Cuts at: 2641 2641
Size: 4253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 673 673
Size: 4253
AlwNI CAG_nnn'CTG
Cuts at: 191 1558 3125 191
Size: 1367 1567 1319
Fragments arranged by size:
1567 1367 1319
ApaI G_GGCC'C
Cuts at: 148 148
Size: 4253
ApaLI G'TGCA_C
Cuts at: 3023 3023
Size: 4253
ApoI r'AATT_y
Cuts at: 471 1257 3606 3862 471
Size: 786 2349 256 862
Fragments arranged by size:
2349 862 786 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4253
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
AvaI C'yCGr_G
Cuts at: 159 893 1273 159
Size: 734 380 3139
Fragments arranged by size:
3139 734 380
BamHI G'GATC_C
Cuts at: 1203 1278 1203
Size: 75 4178
BanI G'GyrC_C
Cuts at: 700 1269 1683 1931 1966 700
Size: 569 414 248 35 2987
Fragments arranged by size:
2987 569 414 248 35
BanII G_rGCy'C
Cuts at: 148 215 260 1267 2297 148
Size: 67 45 1007 1030 2104
Fragments arranged by size:
2104 1030 1007 67 45
BbeI G_GCGC'C
Cuts at: 1687 1935 1687
Size: 248 4005
BbsI GAAGACnn'nnnn_
Cuts at: 385 722 789 945 385
Size: 337 67 156 3693
Fragments arranged by size:
3693 337 156 67
BciVI GTATCCnnnnn_n'
Cuts at: 2151 2912 4253 2151
Size: 761 1341 2151
Fragments arranged by size:
2151 1341 761
BclI T'GATC_A
Cuts at: 356 1773 356
Size: 1417 2836
BglII A'GATC_T
Cuts at: 125 788 1768 3805 125
Size: 663 980 2037 573
Fragments arranged by size:
2037 980 663 573
BlpI GC'TnA_GC
Cuts at: 1365 1365
Size: 4253
Bme1580I G_kGCm'C
Cuts at: 148 1878 1971 3027 148
Size: 1730 93 1056 1374
Fragments arranged by size:
1730 1374 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1465 1546 1881 4025 1465
Size: 81 335 2144 1693
Fragments arranged by size:
2144 1693 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 4253
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 1020 274
Size: 746 3507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 1429 2308 2800 3098 850
Size: 579 879 492 298 2005
Fragments arranged by size:
2005 879 579 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3957 3957
Size: 4253
BsaAI yAC'GTr
Cuts at: 1606 1606
Size: 4253
BsaBI GATnn'nnATC
Cuts at: 6 124 1767 3804 6
Size: 118 1643 2037 455
Fragments arranged by size:
2037 1643 455 118
BsaHI Gr'CG_yC
Cuts at: 719 1684 1932 2634 4130 719
Size: 965 248 702 1496 842
Fragments arranged by size:
1496 965 842 702 248
BsaWI w'CCGG_w
Cuts at: 1442 1963 2641 2915 3062 3473 1442
Size: 521 678 274 147 411 2222
Fragments arranged by size:
2222 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 203 1678 2436 3013 3509 203
Size: 1475 758 577 496 947
Fragments arranged by size:
1475 947 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1795 1841 3049 104
Size: 1691 46 1208 1308
Fragments arranged by size:
1691 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 739 1012 1177 1267 2045 2235 3027 739
Size: 273 165 90 778 190 792 1965
Fragments arranged by size:
1965 792 778 273 190 165 90
BsmBI CGTCTCn'nnnn_
Cuts at: 868 868
Size: 4253
Bsp1286I G_dGCh'C
Cuts at: 148 215 260 739 1012 1177 1267 1878 1971
Size: 67 45 479 273 165 90 611 93
Cuts at: 1971 2045 2235 2297 3027 148
Size: 74 190 62 730 1374
Fragments arranged by size:
1374 730 611 479 273 190 165 93
90 74 67 62 45
BspEI T'CCGG_A
Cuts at: 3473 3473
Size: 4253
BspHI T'CATG_A
Cuts at: 782 3429 782
Size: 2647 1606
BspMI ACCTGCnnnn'nnnn_
Cuts at: 160 920 1303 1517 1819 2200 160
Size: 760 383 214 302 381 2213
Fragments arranged by size:
2213 760 383 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1167 2545 2599 4083 4248 50
Size: 1117 1378 54 1484 165 55
Fragments arranged by size:
1484 1378 1117 165 55 54
BsrDI GCAATG_nn'
Cuts at: 578 2165 578
Size: 1587 2666
BsrFI r'CCGG_y
Cuts at: 232 2251 2432 2641 232
Size: 2019 181 209 1844
Fragments arranged by size:
2019 1844 209 181
BssSI C'ACGA_G
Cuts at: 2524 2882 2524
Size: 358 3895
BstAPI GCAn_nnn'nTGC
Cuts at: 1630 1630
Size: 4253
BstBI TT'CG_AA
Cuts at: 2614 2614
Size: 4253
BstYI r'GATC_y
Cuts at: 1 125 788 1203 1278 1745 1768 2103 2349
Size: 124 663 415 75 467 23 335 246
Cuts at: 2349 3361 3447 3459 3805 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 663 467 449 415 346 335 246
124 86 75 23 12
Bsu36I CC'TnA_GG
Cuts at: 1059 1216 1059
Size: 157 4096
BtgI C'CryG_G
Cuts at: 107 132 107
Size: 25 4228
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1038 2203 2384 2547 1038
Size: 1165 181 163 2744
Fragments arranged by size:
2744 1165 181 163
BtsI GCAGTG_nn'
Cuts at: 1496 1496
Size: 4253
ClaI AT'CG_AT
Cuts at: 1226 1226
Size: 4253
DraI TTT'AAA
Cuts at: 1252 1252
Size: 4253
DraIII CAC_nnn'GTG
Cuts at: 597 597
Size: 4253
DrdI GACnn_nn'nnGTC
Cuts at: 724 1959 2817 724
Size: 1235 858 2160
Fragments arranged by size:
2160 1235 858
EaeI y'GGCC_r
Cuts at: 275 1838 2012 2403 2430 3775 275
Size: 1563 174 391 27 1345 753
Fragments arranged by size:
1563 1345 753 391 174 27
EagI C'GGCC_G
Cuts at: 1838 1838
Size: 4253
EarI CTCTTCn'nnn_
Cuts at: 784 2276 2486 784
Size: 1492 210 2551
Fragments arranged by size:
2551 1492 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2461 2771 2917 4063 2461
Size: 310 146 1146 2651
Fragments arranged by size:
2651 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1265 1265
Size: 4253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 1020 1171 2078 2510 3257 3525 274
Size: 746 151 907 432 747 268 1002
Fragments arranged by size:
1002 907 747 746 432 268 151
EcoNI CCTnn'n_nnAGG
Cuts at: 994 994
Size: 4253
EcoO109I rG'GnC_Cy
Cuts at: 145 774 1392 145
Size: 629 618 3006
Fragments arranged by size:
3006 629 618
EcoRI G'AATT_C
Cuts at: 471 1257 471
Size: 786 3467
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 4253
FspI TGC'GCA
Cuts at: 2034 2034
Size: 4253
HaeII r_GCGC'y
Cuts at: 167 191 233 1687 1935 2957 3936 167
Size: 24 42 1454 248 1022 979 484
Fragments arranged by size:
1454 1022 979 484 248 42 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 748 780 846 878 2095 2125 2127 2157 748
Size: 32 66 32 1217 30 2 30 2844
Fragments arranged by size:
2844 1217 66 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1292 1292
Size: 4253
KasI G'GCGC_C
Cuts at: 1683 1931 1683
Size: 248 4005
KpnI G_GTAC'C
Cuts at: 1273 1273
Size: 4253
MluI A'CGCG_T
Cuts at: 3686 3686
Size: 4253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2924 3108 2924
Size: 184 4069
MscI TGG'CCA
Cuts at: 2014 2014
Size: 4253
MslI CAynn'nnrTG
Cuts at: 352 373 583 850 991 2242 2369 352
Size: 21 210 267 141 1251 127 2236
Fragments arranged by size:
2236 1251 267 210 141 127 21
MspA1I CmG'CkG
Cuts at: 134 252 1027 1364 1678 2038 3051 3296 134
Size: 118 775 337 314 360 1013 245 1091
Fragments arranged by size:
1091 1013 775 360 337 314 245 118
NaeI GCC'GGC
Cuts at: 234 2434 234
Size: 2200 2053
NarI GG'CG_CC
Cuts at: 1684 1932 1684
Size: 248 4005
NcoI C'CATG_G
Cuts at: 107 107
Size: 4253
NgoMIV G'CCGG_C
Cuts at: 232 2432 232
Size: 2200 2053
NspI r_CATG'y
Cuts at: 534 1306 1806 2713 534
Size: 772 500 907 2074
Fragments arranged by size:
2074 907 772 500
PciI A'CATG_T
Cuts at: 530 2709 530
Size: 2179 2074
PfoI T'CCnGG_A
Cuts at: 1130 1130
Size: 4253
PmeI GTTT'AAAC
Cuts at: 1252 1252
Size: 4253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 4221
PpuMI rG'GwC_Cy
Cuts at: 774 774
Size: 4253
PspOMI G'GGCC_C
Cuts at: 144 144
Size: 4253
PstI C_TGCA'G
Cuts at: 1300 1985 1300
Size: 685 3568
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4253
PvuII CAG'CTG
Cuts at: 252 1027 1678 2038 252
Size: 775 651 360 2467
Fragments arranged by size:
2467 775 651 360
RsrII CG'GwC_CG
Cuts at: 2448 2448
Size: 4253
SacI G_AGCT'C
Cuts at: 1267 1267
Size: 4253
SacII CC_GC'GG
Cuts at: 135 135
Size: 4253
SalI G'TCGA_C
Cuts at: 1290 1290
Size: 4253
SapI GCTCTTCn'nnn_
Cuts at: 2276 2486 2276
Size: 210 4043
SbfI CC_TGCA'GG
Cuts at: 1300 1300
Size: 4253
ScaI AGT'ACT
Cuts at: 3770 3770
Size: 4253
SfcI C'TryA_G
Cuts at: 33 1296 1981 2974 3165 33
Size: 1263 685 993 191 1121
Fragments arranged by size:
1263 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1685 1933 1685
Size: 248 4005
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
SmaI CCC'GGG
Cuts at: 1275 1275
Size: 4253
SmlI C'TyrA_G
Cuts at: 865 1408 2323 2815 3077 865
Size: 543 915 492 262 2041
Fragments arranged by size:
2041 915 543 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4253
SphI G_CATG'C
Cuts at: 1306 1806 1306
Size: 500 3753
StyI C'CwwG_G
Cuts at: 107 693 1387 1741 107
Size: 586 694 354 2619
Fragments arranged by size:
2619 694 586 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1836 1969 2428 2637 1836
Size: 133 459 209 3452
Fragments arranged by size:
3452 459 209 133
TatI w'GTAC_w
Cuts at: 3768 3768
Size: 4253
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 481 513 3770 3802 481
Size: 32 3257 32 932
Fragments arranged by size:
3257 932 32 32
Tth111I GACn'n_nGTC
Cuts at: 2050 2050
Size: 4253
XbaI T'CTAG_A
Cuts at: 1284 1284
Size: 4253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 564 933 1748 564
Size: 369 815 3069
Fragments arranged by size:
3069 815 369
XmaI C'CCGG_G
Cuts at: 1273 1273
Size: 4253
ZraI GAC'GTC
Cuts at: 720 720
Size: 4253
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BseRI BsgI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI