Restriction map for
pF1KB6588
(Circular) (Six-base) MAPSORT of: pF1KB6588.seq Check: 8145 from: 1 to: 4331
pF1KB6588 4331 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 398 500 1369 398
Size: 102 869 3360
Fragments arranged by size:
3360 869 102
Acc65I G'GTAC_C
Cuts at: 1347 1347
Size: 4331
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 1199 2156 2588 3335 3603 401
Size: 798 957 432 747 268 1129
Fragments arranged by size:
1129 957 798 747 432 268
AflIII A'CryG_T
Cuts at: 1301 1683 2787 3764 1301
Size: 382 1104 977 1868
Fragments arranged by size:
1868 1104 977 382
AgeI A'CCGG_T
Cuts at: 2719 2719
Size: 4331
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1217 1249 1217
Size: 32 4299
AlwNI CAG_nnn'CTG
Cuts at: 303 688 1177 1204 1636 3203 303
Size: 385 489 27 432 1567 1431
Fragments arranged by size:
1567 1431 489 432 385 27
ApaLI G'TGCA_C
Cuts at: 1194 3101 1194
Size: 1907 2424
ApoI r'AATT_y
Cuts at: 388 772 1054 1335 3684 3940 388
Size: 384 282 281 2349 256 779
Fragments arranged by size:
2349 779 384 282 281 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 707 1124 1351 707
Size: 417 227 3687
Fragments arranged by size:
3687 417 227
BamHI G'GATC_C
Cuts at: 1356 1356
Size: 4331
BanI G'GyrC_C
Cuts at: 1347 1761 2009 2044 1347
Size: 414 248 35 3634
Fragments arranged by size:
3634 414 248 35
BanII G_rGCy'C
Cuts at: 319 629 1157 1345 2375 319
Size: 310 528 188 1030 2275
Fragments arranged by size:
2275 1030 528 310 188
BbeI G_GCGC'C
Cuts at: 1765 2013 1765
Size: 248 4083
BbsI GAAGACnn'nnnn_
Cuts at: 700 700
Size: 4331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 247 281 247
Size: 34 4297
BciVI GTATCCnnnnn_n'
Cuts at: 2229 2990 4331 2229
Size: 761 1341 2229
Fragments arranged by size:
2229 1341 761
BclI T'GATC_A
Cuts at: 1851 1851
Size: 4331
BglII A'GATC_T
Cuts at: 1846 3883 1846
Size: 2037 2294
BlpI GC'TnA_GC
Cuts at: 650 1151 1443 650
Size: 501 292 3538
Fragments arranged by size:
3538 501 292
Bme1580I G_kGCm'C
Cuts at: 1198 1956 2049 3105 1198
Size: 758 93 1056 2424
Fragments arranged by size:
2424 1056 758 93
BmgBI CAC'GTC
Cuts at: 402 402
Size: 4331
BmrI ACTGGGnnnn_n'
Cuts at: 649 698 1543 1624 1959 4103 649
Size: 49 845 81 335 2144 877
Fragments arranged by size:
2144 877 845 335 81 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 333 2698 333
Size: 2365 1966
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 184 665 904 917 949 160
Size: 24 481 239 13 32 3542
Fragments arranged by size:
3542 481 239 32 24 13
Bpu10I CC'TnA_GC
Cuts at: 623 623
Size: 4331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1507 2386 2878 3176 1507
Size: 879 492 298 2662
Fragments arranged by size:
2662 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4035 4035
Size: 4331
BsaAI yAC'GTr
Cuts at: 1684 1684
Size: 4331
BsaBI GATnn'nnATC
Cuts at: 6 1003 1845 3882 6
Size: 997 842 2037 455
Fragments arranged by size:
2037 997 842 455
BsaHI Gr'CG_yC
Cuts at: 1762 2010 2712 4208 1762
Size: 248 702 1496 1885
Fragments arranged by size:
1885 1496 702 248
BsaWI w'CCGG_w
Cuts at: 233 1520 2041 2719 2993 3140 3551 233
Size: 1287 521 678 274 147 411 1013
Fragments arranged by size:
1287 1013 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 304 864 1087 157
Size: 147 560 223 3401
Fragments arranged by size:
3401 560 223 147
BseYI C'CCAG_C
Cuts at: 1756 2514 3091 3587 1756
Size: 758 577 496 2500
Fragments arranged by size:
2500 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 934 460
Size: 474 3857
BsiEI CG_ry'CG
Cuts at: 104 1873 1919 3127 104
Size: 1769 46 1208 1308
Fragments arranged by size:
1769 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 629 1157 1198 1345 2123 2313 3105 629
Size: 528 41 147 778 190 792 1855
Fragments arranged by size:
1855 792 778 528 190 147 41
Bsp1286I G_dGCh'C
Cuts at: 319 629 1157 1198 1345 1956 2049 2123 2313
Size: 310 528 41 147 611 93 74 190
Cuts at: 2313 2375 3105 319
Size: 62 730 1545
Fragments arranged by size:
1545 730 611 528 310 190 147 93
74 62 41
BspEI T'CCGG_A
Cuts at: 233 3551 233
Size: 3318 1013
BspHI T'CATG_A
Cuts at: 3507 3507
Size: 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1381 1595 1897 2278 1381
Size: 214 302 381 3434
Fragments arranged by size:
3434 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2623 2677 4161 4326 50
Size: 2573 54 1484 165 55
Fragments arranged by size:
2573 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 644 2243 644
Size: 1599 2732
BsrFI r'CCGG_y
Cuts at: 2329 2510 2719 2329
Size: 181 209 3941
Fragments arranged by size:
3941 209 181
BssSI C'ACGA_G
Cuts at: 2602 2960 2602
Size: 358 3973
BstAPI GCAn_nnn'nTGC
Cuts at: 1708 1708
Size: 4331
BstBI TT'CG_AA
Cuts at: 632 2692 632
Size: 2060 2271
BstYI r'GATC_y
Cuts at: 1 482 1356 1823 1846 2181 2427 3439 3525
Size: 481 874 467 23 335 246 1012 86
Cuts at: 3525 3537 3883 1
Size: 12 346 449
Fragments arranged by size:
1012 874 481 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 344 107
Size: 237 4094
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2281 2462 2625 2281
Size: 181 163 3987
Fragments arranged by size:
3987 181 163
BtsI GCAGTG_nn'
Cuts at: 1018 1574 1018
Size: 556 3775
DraI TTT'AAA
Cuts at: 1330 1330
Size: 4331
DrdI GACnn_nn'nnGTC
Cuts at: 2037 2895 2037
Size: 858 3473
EaeI y'GGCC_r
Cuts at: 1916 2090 2481 2508 3853 1916
Size: 174 391 27 1345 2394
Fragments arranged by size:
2394 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1916 1916
Size: 4331
EarI CTCTTCn'nnn_
Cuts at: 634 2354 2564 634
Size: 1720 210 2401
Fragments arranged by size:
2401 1720 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 157 2539 2849 2995 4141 157
Size: 2382 310 146 1146 347
Fragments arranged by size:
2382 1146 347 310 146
EcoICRI GAG'CTC
Cuts at: 627 1155 1343 627
Size: 528 188 3615
Fragments arranged by size:
3615 528 188
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 184 401 665 904 917 949 1199 2156
Size: 24 217 264 239 13 32 250 957
Cuts at: 2156 2588 3335 3603 160
Size: 432 747 268 888
Fragments arranged by size:
957 888 747 432 268 264 250 239
217 32 24 13
EcoO109I rG'GnC_Cy
Cuts at: 1470 1470
Size: 4331
EcoRI G'AATT_C
Cuts at: 1054 1335 1054
Size: 281 4050
EcoRV GAT'ATC
Cuts at: 1005 1005
Size: 4331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 4331
FspI TGC'GCA
Cuts at: 2112 2112
Size: 4331
HaeII r_GCGC'y
Cuts at: 115 1765 2013 3035 4014 115
Size: 1650 248 1022 979 432
Fragments arranged by size:
1650 1022 979 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1089 1121 1223 1255 2173 2203 2205 2235 1089
Size: 32 102 32 918 30 2 30 3185
Fragments arranged by size:
3185 918 102 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1370 1370
Size: 4331
KasI G'GCGC_C
Cuts at: 1761 2009 1761
Size: 248 4083
KpnI G_GTAC'C
Cuts at: 1351 1351
Size: 4331
MfeI C'AATT_G
Cuts at: 438 438
Size: 4331
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3002 3186 3002
Size: 184 4147
MscI TGG'CCA
Cuts at: 2092 2092
Size: 4331
MslI CAynn'nnrTG
Cuts at: 847 2320 2447 847
Size: 1473 127 2731
Fragments arranged by size:
2731 1473 127
MspA1I CmG'CkG
Cuts at: 161 204 300 1442 1756 2116 3129 3374 161
Size: 43 96 1142 314 360 1013 245 1118
Fragments arranged by size:
1142 1118 1013 360 314 245 96 43
NaeI GCC'GGC
Cuts at: 2512 2512
Size: 4331
NarI GG'CG_CC
Cuts at: 1762 2010 1762
Size: 248 4083
NcoI C'CATG_G
Cuts at: 107 344 107
Size: 237 4094
NgoMIV G'CCGG_C
Cuts at: 2510 2510
Size: 4331
NspI r_CATG'y
Cuts at: 832 1212 1305 1384 1884 2791 832
Size: 380 93 79 500 907 2372
Fragments arranged by size:
2372 907 500 380 93 79
PasI CC'CwG_GG
Cuts at: 1030 1030
Size: 4331
PciI A'CATG_T
Cuts at: 1301 2787 1301
Size: 1486 2845
PflMI CCAn_nnn'nTGG
Cuts at: 688 688
Size: 4331
PfoI T'CCnGG_A
Cuts at: 1007 1007
Size: 4331
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 4299
PstI C_TGCA'G
Cuts at: 245 308 1378 2063 245
Size: 63 1070 685 2513
Fragments arranged by size:
2513 1070 685 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 204 300 1756 2116 204
Size: 96 1456 360 2419
Fragments arranged by size:
2419 1456 360 96
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 629 1157 1345 629
Size: 528 188 3615
Fragments arranged by size:
3615 528 188
SalI G'TCGA_C
Cuts at: 1368 1368
Size: 4331
SapI GCTCTTCn'nnn_
Cuts at: 634 2354 2564 634
Size: 1720 210 2401
Fragments arranged by size:
2401 1720 210
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 3848 3848
Size: 4331
SfcI C'TryA_G
Cuts at: 33 241 295 304 1374 2059 3052 3243 33
Size: 208 54 9 1070 685 993 191 1121
Fragments arranged by size:
1121 1070 993 685 208 191 54 9
SfoI GGC'GCC
Cuts at: 1763 2011 1763
Size: 248 4083
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SmaI CCC'GGG
Cuts at: 709 1126 1353 709
Size: 417 227 3687
Fragments arranged by size:
3687 417 227
SmlI C'TyrA_G
Cuts at: 1486 2401 2893 3155 1486
Size: 915 492 262 2662
Fragments arranged by size:
2662 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 1384 1884 1384
Size: 500 3831
SrfI GCCC'GGGC
Cuts at: 709 709
Size: 4331
SspI AAT'ATT
Cuts at: 175 175
Size: 4331
StyI C'CwwG_G
Cuts at: 107 344 656 1271 1465 1819 107
Size: 237 312 615 194 354 2619
Fragments arranged by size:
2619 615 354 312 237 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 450 1914 2047 2506 2715 450
Size: 1464 133 459 209 2066
Fragments arranged by size:
2066 1464 459 209 133
TatI w'GTAC_w
Cuts at: 3846 3846
Size: 4331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 4299
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 444 1826 444
Size: 1382 2949
XmaI C'CCGG_G
Cuts at: 707 1124 1351 707
Size: 417 227 3687
Fragments arranged by size:
3687 417 227
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI StuI SwaI XhoI XmnI
ZraI