Restriction map for
pF1KB6587
(Circular) (Six-base) MAPSORT of: pF1KB6587.seq Check: 8894 from: 1 to: 4178
pF1KB6587 4178 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1216 1216
Size: 4178
Acc65I G'GTAC_C
Cuts at: 1194 1194
Size: 4178
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 2003 2435 3182 3450 751
Size: 1252 432 747 268 1479
Fragments arranged by size:
1479 1252 747 432 268
AflIII A'CryG_T
Cuts at: 912 1530 2634 3611 912
Size: 618 1104 977 1479
Fragments arranged by size:
1479 1104 977 618
AgeI A'CCGG_T
Cuts at: 2566 2566
Size: 4178
AlwNI CAG_nnn'CTG
Cuts at: 255 1483 3050 255
Size: 1228 1567 1383
Fragments arranged by size:
1567 1383 1228
ApaLI G'TGCA_C
Cuts at: 2948 2948
Size: 4178
ApoI r'AATT_y
Cuts at: 1182 3531 3787 1182
Size: 2349 256 1573
Fragments arranged by size:
2349 1573 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 133 304 1198 133
Size: 171 894 3113
Fragments arranged by size:
3113 894 171
BamHI G'GATC_C
Cuts at: 342 836 1203 342
Size: 494 367 3317
Fragments arranged by size:
3317 494 367
BanI G'GyrC_C
Cuts at: 1194 1608 1856 1891 1194
Size: 414 248 35 3481
Fragments arranged by size:
3481 414 248 35
BanII G_rGCy'C
Cuts at: 211 509 1192 2222 211
Size: 298 683 1030 2167
Fragments arranged by size:
2167 1030 683 298
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BbsI GAAGACnn'nnnn_
Cuts at: 287 287
Size: 4178
BciVI GTATCCnnnnn_n'
Cuts at: 810 1081 2076 2837 4178 810
Size: 271 995 761 1341 810
Fragments arranged by size:
1341 995 810 761 271
BclI T'GATC_A
Cuts at: 1152 1698 1152
Size: 546 3632
BglI GCCn_nnn'nGGC
Cuts at: 817 817
Size: 4178
BglII A'GATC_T
Cuts at: 1693 3730 1693
Size: 2037 2141
BlpI GC'TnA_GC
Cuts at: 1290 1290
Size: 4178
Bme1580I G_kGCm'C
Cuts at: 599 1803 1896 2952 599
Size: 1204 93 1056 1825
Fragments arranged by size:
1825 1204 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1390 1471 1806 3950 1390
Size: 81 335 2144 1618
Fragments arranged by size:
2144 1618 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 4178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 789 826 187
Size: 602 37 3539
Fragments arranged by size:
3539 602 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 1354 2233 2725 3023 589
Size: 765 879 492 298 1744
Fragments arranged by size:
1744 879 765 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 328 3882 328
Size: 3554 624
BsaAI yAC'GTr
Cuts at: 1531 1531
Size: 4178
BsaBI GATnn'nnATC
Cuts at: 6 1692 3729 6
Size: 1686 2037 455
Fragments arranged by size:
2037 1686 455
BsaHI Gr'CG_yC
Cuts at: 1098 1609 1857 2559 4055 1098
Size: 511 248 702 1496 1221
Fragments arranged by size:
1496 1221 702 511 248
BsaWI w'CCGG_w
Cuts at: 1367 1888 2566 2840 2987 3398 1367
Size: 521 678 274 147 411 2147
Fragments arranged by size:
2147 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 610 640 610
Size: 30 4148
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 322 337 486 489 498 307
Size: 15 15 149 3 9 3987
Fragments arranged by size:
3987 149 15 15 9 3
BseYI C'CCAG_C
Cuts at: 145 270 442 826 864 995 1603 2361 2938
Size: 125 172 384 38 131 608 758 577
Cuts at: 2938 3434 145
Size: 496 889
Fragments arranged by size:
889 758 608 577 496 384 172 131
125 38
BsiEI CG_ry'CG
Cuts at: 104 1720 1766 2974 104
Size: 1616 46 1208 1308
Fragments arranged by size:
1616 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 211 1192 1970 2160 2952 211
Size: 981 778 190 792 1437
Fragments arranged by size:
1437 981 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 211 509 599 1192 1803 1896 1970 2160 2222
Size: 298 90 593 611 93 74 190 62
Cuts at: 2222 2952 211
Size: 730 1437
Fragments arranged by size:
1437 730 611 593 298 190 93 90
74 62
BspEI T'CCGG_A
Cuts at: 3398 3398
Size: 4178
BspHI T'CATG_A
Cuts at: 3354 3354
Size: 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 155 1228 1442 1744 2125 155
Size: 1073 214 302 381 2208
Fragments arranged by size:
2208 1073 381 302 214
BsrBI CCG'CTC
Cuts at: 50 561 2470 2524 4008 4173 50
Size: 511 1909 54 1484 165 55
Fragments arranged by size:
1909 1484 511 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2090 2090
Size: 4178
BsrFI r'CCGG_y
Cuts at: 224 416 2176 2357 2566 224
Size: 192 1760 181 209 1836
Fragments arranged by size:
1836 1760 209 192 181
BssSI C'ACGA_G
Cuts at: 2449 2807 2449
Size: 358 3820
BstAPI GCAn_nnn'nTGC
Cuts at: 1555 1555
Size: 4178
BstBI TT'CG_AA
Cuts at: 2539 2539
Size: 4178
BstXI CCAn_nnnn'nTGG
Cuts at: 876 1023 876
Size: 147 4031
BstYI r'GATC_y
Cuts at: 1 342 836 987 1203 1670 1693 2028 2274
Size: 341 494 151 216 467 23 335 246
Cuts at: 2274 3286 3372 3384 3730 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 494 467 449 346 341 335 246
216 151 86 23 12
BtgI C'CryG_G
Cuts at: 107 195 599 107
Size: 88 404 3686
Fragments arranged by size:
3686 404 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2128 2309 2472 2128
Size: 181 163 3834
Fragments arranged by size:
3834 181 163
BtsI GCAGTG_nn'
Cuts at: 1421 1421
Size: 4178
DraI TTT'AAA
Cuts at: 1177 1177
Size: 4178
DrdI GACnn_nn'nnGTC
Cuts at: 1884 2742 1884
Size: 858 3320
EaeI y'GGCC_r
Cuts at: 314 740 944 1028 1052 1763 1937 2328 2355
Size: 426 204 84 24 711 174 391 27
Cuts at: 2355 3700 314
Size: 1345 792
Fragments arranged by size:
1345 792 711 426 391 204 174 84
27 24
EagI C'GGCC_G
Cuts at: 1763 1763
Size: 4178
EarI CTCTTCn'nnn_
Cuts at: 284 389 463 875 2201 2411 284
Size: 105 74 412 1326 210 2051
Fragments arranged by size:
2051 1326 412 210 105 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 495 2386 2696 2842 3988 495
Size: 1891 310 146 1146 685
Fragments arranged by size:
1891 1146 685 310 146
EcoICRI GAG'CTC
Cuts at: 209 1190 209
Size: 981 3197
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 751 789 826 2003 2435 3182 3450 187
Size: 564 38 37 1177 432 747 268 915
Fragments arranged by size:
1177 915 747 564 432 268 38 37
EcoNI CCTnn'n_nnAGG
Cuts at: 382 1081 382
Size: 699 3479
EcoO109I rG'GnC_Cy
Cuts at: 252 539 861 888 1074 1317 252
Size: 287 322 27 186 243 3113
Fragments arranged by size:
3113 322 287 243 186 27
EcoRI G'AATT_C
Cuts at: 1182 1182
Size: 4178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 313 373 2197 313
Size: 60 1824 2294
Fragments arranged by size:
2294 1824 60
FspI TGC'GCA
Cuts at: 1069 1959 1069
Size: 890 3288
HaeII r_GCGC'y
Cuts at: 1612 1860 2882 3861 1612
Size: 248 1022 979 1929
Fragments arranged by size:
1929 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 327 359 526 558 610 642 2020 2050 2052
Size: 32 167 32 52 32 1378 30 2
Cuts at: 2052 2082 327
Size: 30 2423
Fragments arranged by size:
2423 1378 167 52 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1217 1217
Size: 4178
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 1198 1198
Size: 4178
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 149 347 944 2849 3033 149
Size: 198 597 1905 184 1294
Fragments arranged by size:
1905 1294 597 198 184
MscI TGG'CCA
Cuts at: 946 1030 1054 1939 946
Size: 84 24 885 3185
Fragments arranged by size:
3185 885 84 24
MslI CAynn'nnrTG
Cuts at: 451 592 778 2167 2294 451
Size: 141 186 1389 127 2335
Fragments arranged by size:
2335 1389 186 141 127
MspA1I CmG'CkG
Cuts at: 601 1289 1603 1963 2976 3221 601
Size: 688 314 360 1013 245 1558
Fragments arranged by size:
1558 1013 688 360 314 245
NaeI GCC'GGC
Cuts at: 226 418 2359 226
Size: 192 1941 2045
Fragments arranged by size:
2045 1941 192
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NcoI C'CATG_G
Cuts at: 107 107
Size: 4178
NgoMIV G'CCGG_C
Cuts at: 224 416 2357 224
Size: 192 1941 2045
Fragments arranged by size:
2045 1941 192
NspI r_CATG'y
Cuts at: 777 916 1231 1731 2638 777
Size: 139 315 500 907 2317
Fragments arranged by size:
2317 907 500 315 139
PciI A'CATG_T
Cuts at: 912 2634 912
Size: 1722 2456
PflMI CCAn_nnn'nTGG
Cuts at: 255 255
Size: 4178
PfoI T'CCnGG_A
Cuts at: 329 377 329
Size: 48 4130
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PpuMI rG'GwC_Cy
Cuts at: 252 888 252
Size: 636 3542
PshAI GACnn'nnGTC
Cuts at: 679 1097 679
Size: 418 3760
PstI C_TGCA'G
Cuts at: 1225 1910 1225
Size: 685 3493
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 1603 1963 1603
Size: 360 3818
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 211 1192 211
Size: 981 3197
SacII CC_GC'GG
Cuts at: 602 602
Size: 4178
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SanDI GG'GwC_CC
Cuts at: 888 888
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 2201 2411 2201
Size: 210 3968
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 3695 3695
Size: 4178
SfcI C'TryA_G
Cuts at: 33 1221 1906 2899 3090 33
Size: 1188 685 993 191 1121
Fragments arranged by size:
1188 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 1200 1200
Size: 4178
SmlI C'TyrA_G
Cuts at: 568 1333 2248 2740 3002 568
Size: 765 915 492 262 1744
Fragments arranged by size:
1744 915 765 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 777 1231 1731 777
Size: 454 500 3224
Fragments arranged by size:
3224 500 454
SspI AAT'ATT
Cuts at: 655 655
Size: 4178
StuI AGG'CCT
Cuts at: 1164 1164
Size: 4178
StyI C'CwwG_G
Cuts at: 107 1312 1666 107
Size: 1205 354 2619
Fragments arranged by size:
2619 1205 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 571 686 998 1761 1894 2353 2562 571
Size: 115 312 763 133 459 209 2187
Fragments arranged by size:
2187 763 459 312 209 133 115
TatI w'GTAC_w
Cuts at: 548 3693 548
Size: 3145 1033
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4146
Tth111I GACn'n_nGTC
Cuts at: 1975 1975
Size: 4178
XbaI T'CTAG_A
Cuts at: 1209 1209
Size: 4178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1023 1673 1023
Size: 650 3528
XmaI C'CCGG_G
Cuts at: 1198 1198
Size: 4178
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PsiI PspOMI PspXI PsrI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI
ZraI