Restriction map for
pF1KB6582
(Circular) (Six-base) MAPSORT of: pF1KB6582.seq Check: 3646 from: 1 to: 4325
pF1KB6582 4325 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1363 1363
Size: 4325
Acc65I G'GTAC_C
Cuts at: 1341 1341
Size: 4325
AclI AA'CG_TT
Cuts at: 4165 4165
Size: 4325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 2150 2582 3329 3597 481
Size: 1669 432 747 268 1209
Fragments arranged by size:
1669 1209 747 432 268
AflIII A'CryG_T
Cuts at: 1677 2781 3758 1677
Size: 1104 977 2244
Fragments arranged by size:
2244 1104 977
AgeI A'CCGG_T
Cuts at: 2713 2713
Size: 4325
AhdI GACnn_n'nnGTC
Cuts at: 342 342
Size: 4325
AlwNI CAG_nnn'CTG
Cuts at: 282 1630 3197 282
Size: 1348 1567 1410
Fragments arranged by size:
1567 1410 1348
ApaI G_GGCC'C
Cuts at: 1226 1226
Size: 4325
ApaLI G'TGCA_C
Cuts at: 3095 3095
Size: 4325
ApoI r'AATT_y
Cuts at: 199 1329 3678 3934 199
Size: 1130 2349 256 590
Fragments arranged by size:
2349 1130 590 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4325
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
AvaI C'yCGr_G
Cuts at: 753 1312 1345 753
Size: 559 33 3733
Fragments arranged by size:
3733 559 33
BamHI G'GATC_C
Cuts at: 1350 1350
Size: 4325
BanI G'GyrC_C
Cuts at: 1341 1755 2003 2038 1341
Size: 414 248 35 3628
Fragments arranged by size:
3628 414 248 35
BanII G_rGCy'C
Cuts at: 137 1226 1339 2369 137
Size: 1089 113 1030 2093
Fragments arranged by size:
2093 1089 1030 113
BbeI G_GCGC'C
Cuts at: 1759 2007 1759
Size: 248 4077
BbvCI CC'TCA_GC
Cuts at: 1155 1155
Size: 4325
BciVI GTATCCnnnnn_n'
Cuts at: 2223 2984 4325 2223
Size: 761 1341 2223
Fragments arranged by size:
2223 1341 761
BclI T'GATC_A
Cuts at: 537 1845 537
Size: 1308 3017
BglII A'GATC_T
Cuts at: 947 1840 3877 947
Size: 893 2037 1395
Fragments arranged by size:
2037 1395 893
BlpI GC'TnA_GC
Cuts at: 1437 1437
Size: 4325
Bme1580I G_kGCm'C
Cuts at: 887 1226 1950 2043 3099 887
Size: 339 724 93 1056 2113
Fragments arranged by size:
2113 1056 724 339 93
BmrI ACTGGGnnnn_n'
Cuts at: 1537 1618 1953 4097 1537
Size: 81 335 2144 1765
Fragments arranged by size:
2144 1765 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1275 1275
Size: 4325
Bpu10I CC'TnA_GC
Cuts at: 1155 1155
Size: 4325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 1501 2380 2872 3170 539
Size: 962 879 492 298 1694
Fragments arranged by size:
1694 962 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 409 4029 409
Size: 3620 705
BsaAI yAC'GTr
Cuts at: 1678 1678
Size: 4325
BsaBI GATnn'nnATC
Cuts at: 6 449 1839 3876 6
Size: 443 1390 2037 455
Fragments arranged by size:
2037 1390 455 443
BsaHI Gr'CG_yC
Cuts at: 326 1756 2004 2706 4202 326
Size: 1430 248 702 1496 449
Fragments arranged by size:
1496 1430 702 449 248
BsaWI w'CCGG_w
Cuts at: 1514 2035 2713 2987 3134 3545 1514
Size: 521 678 274 147 411 2294
Fragments arranged by size:
2294 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 364
Size: 4325
BseYI C'CCAG_C
Cuts at: 243 1750 2508 3085 3581 243
Size: 1507 758 577 496 987
Fragments arranged by size:
1507 987 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1867 1913 3121 104
Size: 1763 46 1208 1308
Fragments arranged by size:
1763 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1339 2117 2307 3099 1339
Size: 778 190 792 2565
Fragments arranged by size:
2565 792 778 190
BsmI GAATG_Cn'
Cuts at: 1204 1204
Size: 4325
Bsp1286I G_dGCh'C
Cuts at: 137 887 1226 1339 1950 2043 2117 2307 2369
Size: 750 339 113 611 93 74 190 62
Cuts at: 2369 3099 137
Size: 730 1363
Fragments arranged by size:
1363 750 730 611 339 190 113 93
74 62
BspEI T'CCGG_A
Cuts at: 3545 3545
Size: 4325
BspHI T'CATG_A
Cuts at: 1238 3501 1238
Size: 2263 2062
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1308 1375 1589 1891 2272 1308
Size: 67 214 302 381 3361
Fragments arranged by size:
3361 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 692 2617 2671 4155 4320 50
Size: 642 1925 54 1484 165 55
Fragments arranged by size:
1925 1484 642 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2237 2237
Size: 4325
BsrFI r'CCGG_y
Cuts at: 2323 2504 2713 2323
Size: 181 209 3935
Fragments arranged by size:
3935 209 181
BsrGI T'GTAC_A
Cuts at: 743 1197 743
Size: 454 3871
BssSI C'ACGA_G
Cuts at: 2596 2954 2596
Size: 358 3967
BstAPI GCAn_nnn'nTGC
Cuts at: 1702 1702
Size: 4325
BstBI TT'CG_AA
Cuts at: 2686 2686
Size: 4325
BstEII G'GTnAC_C
Cuts at: 1131 1131
Size: 4325
BstYI r'GATC_y
Cuts at: 1 122 450 947 1299 1350 1817 1840 2175
Size: 121 328 497 352 51 467 23 335
Cuts at: 2175 2421 3433 3519 3531 3877 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 497 467 449 352 346 335 328
246 121 86 51 23 12
BtgI C'CryG_G
Cuts at: 714 714
Size: 4325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2275 2456 2619 2275
Size: 181 163 3981
Fragments arranged by size:
3981 181 163
BtsI GCAGTG_nn'
Cuts at: 212 1568 212
Size: 1356 2969
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1047 1082 1047
Size: 35 4290
DraI TTT'AAA
Cuts at: 1324 1324
Size: 4325
DrdI GACnn_nn'nnGTC
Cuts at: 2031 2889 2031
Size: 858 3467
EaeI y'GGCC_r
Cuts at: 1910 2084 2475 2502 3847 1910
Size: 174 391 27 1345 2388
Fragments arranged by size:
2388 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1910 1910
Size: 4325
EarI CTCTTCn'nnn_
Cuts at: 431 844 1237 2348 2558 431
Size: 413 393 1111 210 2198
Fragments arranged by size:
2198 1111 413 393 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2533 2843 2989 4135 2533
Size: 310 146 1146 2723
Fragments arranged by size:
2723 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1337 1337
Size: 4325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 1275 2150 2582 3329 3597 481
Size: 794 875 432 747 268 1209
Fragments arranged by size:
1209 875 794 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 229 1315 229
Size: 1086 3239
EcoO109I rG'GnC_Cy
Cuts at: 1222 1223 1464 1222
Size: 1 241 4083
Fragments arranged by size:
4083 241 1
EcoRI G'AATT_C
Cuts at: 199 1329 199
Size: 1130 3195
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 808 2344 808
Size: 1536 2789
FspI TGC'GCA
Cuts at: 2106 2106
Size: 4325
HaeII r_GCGC'y
Cuts at: 1759 2007 3029 4008 1759
Size: 248 1022 979 2076
Fragments arranged by size:
2076 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 328 360 389 421 932 964 2167 2197 2199
Size: 32 29 32 511 32 1203 30 2
Cuts at: 2199 2229 328
Size: 30 2424
Fragments arranged by size:
2424 1203 511 32 32 32 30 30
29 2
HincII GTy'rAC
Cuts at: 1364 1364
Size: 4325
HindIII A'AGCT_T
Cuts at: 503 503
Size: 4325
KasI G'GCGC_C
Cuts at: 1755 2003 1755
Size: 248 4077
KpnI G_GTAC'C
Cuts at: 1345 1345
Size: 4325
MluI A'CGCG_T
Cuts at: 3758 3758
Size: 4325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 326 1149 2996 3180 326
Size: 823 1847 184 1471
Fragments arranged by size:
1847 1471 823 184
MscI TGG'CCA
Cuts at: 2086 2086
Size: 4325
MslI CAynn'nnrTG
Cuts at: 2314 2441 2314
Size: 127 4198
MspA1I CmG'CkG
Cuts at: 1094 1272 1436 1750 2110 3123 3368 1094
Size: 178 164 314 360 1013 245 2051
Fragments arranged by size:
2051 1013 360 314 245 178 164
NaeI GCC'GGC
Cuts at: 2506 2506
Size: 4325
NarI GG'CG_CC
Cuts at: 1756 2004 1756
Size: 248 4077
NgoMIV G'CCGG_C
Cuts at: 2504 2504
Size: 4325
NspI r_CATG'y
Cuts at: 604 1378 1878 2785 604
Size: 774 500 907 2144
Fragments arranged by size:
2144 907 774 500
PciI A'CATG_T
Cuts at: 2781 2781
Size: 4325
PflMI CCAn_nnn'nTGG
Cuts at: 282 282
Size: 4325
PmeI GTTT'AAAC
Cuts at: 1324 1324
Size: 4325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 4293
PspOMI G'GGCC_C
Cuts at: 1222 1222
Size: 4325
PstI C_TGCA'G
Cuts at: 152 1372 2057 152
Size: 1220 685 2420
Fragments arranged by size:
2420 1220 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4325
PvuII CAG'CTG
Cuts at: 1272 1750 2110 1272
Size: 478 360 3487
Fragments arranged by size:
3487 478 360
RsrII CG'GwC_CG
Cuts at: 312 2520 312
Size: 2208 2117
SacI G_AGCT'C
Cuts at: 1339 1339
Size: 4325
SalI G'TCGA_C
Cuts at: 1362 1362
Size: 4325
SapI GCTCTTCn'nnn_
Cuts at: 2348 2558 2348
Size: 210 4115
SbfI CC_TGCA'GG
Cuts at: 1372 1372
Size: 4325
ScaI AGT'ACT
Cuts at: 214 1111 3842 214
Size: 897 2731 697
Fragments arranged by size:
2731 897 697
SfcI C'TryA_G
Cuts at: 33 148 1368 2053 3046 3237 33
Size: 115 1220 685 993 191 1121
Fragments arranged by size:
1220 1121 993 685 191 115
SfoI GGC'GCC
Cuts at: 1757 2005 1757
Size: 248 4077
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
SmaI CCC'GGG
Cuts at: 1347 1347
Size: 4325
SmlI C'TyrA_G
Cuts at: 554 1480 2395 2887 3149 554
Size: 926 915 492 262 1730
Fragments arranged by size:
1730 926 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4325
SphI G_CATG'C
Cuts at: 1378 1878 1378
Size: 500 3825
StyI C'CwwG_G
Cuts at: 454 612 1459 1813 454
Size: 158 847 354 2966
Fragments arranged by size:
2966 847 354 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1908 2041 2500 2709 1908
Size: 133 459 209 3524
Fragments arranged by size:
3524 459 209 133
TatI w'GTAC_w
Cuts at: 212 743 1109 1197 3840 212
Size: 531 366 88 2643 697
Fragments arranged by size:
2643 697 531 366 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3842 3874 3842
Size: 32 4293
Tth111I GACn'n_nGTC
Cuts at: 193 316 2122 193
Size: 123 1806 2396
Fragments arranged by size:
2396 1806 123
XbaI T'CTAG_A
Cuts at: 1356 1356
Size: 4325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 619 1299 1820 619
Size: 680 521 3124
Fragments arranged by size:
3124 680 521
XmaI C'CCGG_G
Cuts at: 1345 1345
Size: 4325
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsgI BsiWI BsmBI BssHII BstXI BstZ17I Bsu36I
ClaI DraIII EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI