Restriction map for
pF1KB6575
(Circular) (Six-base) MAPSORT of: pF1KB6575.seq Check: 3114 from: 1 to: 4241
pF1KB6575 4241 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 553 553
Size: 4241
AccI GT'mk_AC
Cuts at: 617 1279 617
Size: 662 3579
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 2066 2498 3245 3513 631
Size: 1435 432 747 268 1359
Fragments arranged by size:
1435 1359 747 432 268
AflIII A'CryG_T
Cuts at: 1593 2697 3674 1593
Size: 1104 977 2160
Fragments arranged by size:
2160 1104 977
AgeI A'CCGG_T
Cuts at: 2629 2629
Size: 4241
AlwNI CAG_nnn'CTG
Cuts at: 1546 3113 1546
Size: 1567 2674
ApaLI G'TGCA_C
Cuts at: 482 3011 482
Size: 2529 1712
ApoI r'AATT_y
Cuts at: 473 1245 3594 3850 473
Size: 772 2349 256 864
Fragments arranged by size:
2349 864 772 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 1261 1261
Size: 4241
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1142 1175 1142
Size: 33 4208
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 1257 1671 1919 1954 1257
Size: 414 248 35 3544
Fragments arranged by size:
3544 414 248 35
BanII G_rGCy'C
Cuts at: 1255 2285 1255
Size: 1030 3211
BbeI G_GCGC'C
Cuts at: 1675 1923 1675
Size: 248 3993
BbsI GAAGACnn'nnnn_
Cuts at: 124 124
Size: 4241
BbvCI CC'TCA_GC
Cuts at: 212 212
Size: 4241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4207
BciVI GTATCCnnnnn_n'
Cuts at: 2139 2900 4241 2139
Size: 761 1341 2139
Fragments arranged by size:
2139 1341 761
BclI T'GATC_A
Cuts at: 914 1761 914
Size: 847 3394
BglII A'GATC_T
Cuts at: 1756 3793 1756
Size: 2037 2204
BlpI GC'TnA_GC
Cuts at: 244 1353 244
Size: 1109 3132
Bme1580I G_kGCm'C
Cuts at: 455 486 1866 1959 3015 455
Size: 31 1380 93 1056 1681
Fragments arranged by size:
1681 1380 1056 93 31
BmrI ACTGGGnnnn_n'
Cuts at: 1453 1534 1869 4013 1453
Size: 81 335 2144 1681
Fragments arranged by size:
2144 1681 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 328 2608 328
Size: 2280 1961
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 152
Size: 4241
Bpu10I CC'TnA_GC
Cuts at: 212 212
Size: 4241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 1215 1417 2296 2788 3086 400
Size: 815 202 879 492 298 1555
Fragments arranged by size:
1555 879 815 492 298 202
BsaI GGTCTCn'nnnn_
Cuts at: 1211 3945 1211
Size: 2734 1507
BsaAI yAC'GTr
Cuts at: 1594 1594
Size: 4241
BsaBI GATnn'nnATC
Cuts at: 6 983 1755 3792 6
Size: 977 772 2037 455
Fragments arranged by size:
2037 977 772 455
BsaHI Gr'CG_yC
Cuts at: 1672 1920 2622 4118 1672
Size: 248 702 1496 1795
Fragments arranged by size:
1795 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1430 1951 2629 2903 3050 3461 1430
Size: 521 678 274 147 411 2210
Fragments arranged by size:
2210 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 569 569
Size: 4241
BseYI C'CCAG_C
Cuts at: 905 1096 1666 2424 3001 3497 905
Size: 191 570 758 577 496 1649
Fragments arranged by size:
1649 758 577 570 496 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 553
Size: 4241
BsiEI CG_ry'CG
Cuts at: 104 1783 1829 3037 104
Size: 1679 46 1208 1308
Fragments arranged by size:
1679 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 486 1201 1255 2033 2223 3015 486
Size: 715 54 778 190 792 1712
Fragments arranged by size:
1712 792 778 715 190 54
BsmI GAATG_Cn'
Cuts at: 635 1114 635
Size: 479 3762
Bsp1286I G_dGCh'C
Cuts at: 455 486 1201 1255 1866 1959 2033 2223 2285
Size: 31 715 54 611 93 74 190 62
Cuts at: 2285 3015 455
Size: 730 1681
Fragments arranged by size:
1681 730 715 611 190 93 74 62
54 31
BspEI T'CCGG_A
Cuts at: 3461 3461
Size: 4241
BspHI T'CATG_A
Cuts at: 855 3417 855
Size: 2562 1679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 553 1291 1505 1807 2188 553
Size: 738 214 302 381 2606
Fragments arranged by size:
2606 738 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2533 2587 4071 4236 50
Size: 2483 54 1484 165 55
Fragments arranged by size:
2483 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1174 2153 1174
Size: 979 3262
BsrFI r'CCGG_y
Cuts at: 2239 2420 2629 2239
Size: 181 209 3851
Fragments arranged by size:
3851 209 181
BsrGI T'GTAC_A
Cuts at: 361 1085 361
Size: 724 3517
BssSI C'ACGA_G
Cuts at: 1214 2512 2870 1214
Size: 1298 358 2585
Fragments arranged by size:
2585 1298 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1618 1618
Size: 4241
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstYI r'GATC_y
Cuts at: 1 1266 1733 1756 2091 2337 3349 3435 3447
Size: 1265 467 23 335 246 1012 86 12
Cuts at: 3447 3793 1
Size: 346 449
Fragments arranged by size:
1265 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1136 1136
Size: 4241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2191 2372 2535 2191
Size: 181 163 3897
Fragments arranged by size:
3897 181 163
BtsI GCAGTG_nn'
Cuts at: 1484 1484
Size: 4241
DraI TTT'AAA
Cuts at: 1240 1240
Size: 4241
DraIII CAC_nnn'GTG
Cuts at: 490 490
Size: 4241
DrdI GACnn_nn'nnGTC
Cuts at: 1947 2805 1947
Size: 858 3383
EaeI y'GGCC_r
Cuts at: 1826 2000 2391 2418 3763 1826
Size: 174 391 27 1345 2304
Fragments arranged by size:
2304 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 297 881 2264 2474 297
Size: 584 1383 210 2064
Fragments arranged by size:
2064 1383 584 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2449 2759 2905 4051 2449
Size: 310 146 1146 2639
Fragments arranged by size:
2639 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1253 1253
Size: 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 631 2066 2498 3245 3513 152
Size: 479 1435 432 747 268 880
Fragments arranged by size:
1435 880 747 479 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1007 1007
Size: 4241
EcoO109I rG'GnC_Cy
Cuts at: 1380 1380
Size: 4241
EcoRI G'AATT_C
Cuts at: 1245 1245
Size: 4241
EcoRV GAT'ATC
Cuts at: 263 263
Size: 4241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 796 2260 796
Size: 1464 2777
FspI TGC'GCA
Cuts at: 2022 2022
Size: 4241
HaeII r_GCGC'y
Cuts at: 1675 1923 2945 3924 1675
Size: 248 1022 979 1992
Fragments arranged by size:
1992 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 856 888 2083 2113 2115 2145 856
Size: 32 1195 30 2 30 2952
Fragments arranged by size:
2952 1195 32 30 30 2
HincII GTy'rAC
Cuts at: 1280 1280
Size: 4241
HindIII A'AGCT_T
Cuts at: 689 689
Size: 4241
KasI G'GCGC_C
Cuts at: 1671 1919 1671
Size: 248 3993
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 474 2912 3096 474
Size: 2438 184 1619
Fragments arranged by size:
2438 1619 184
MscI TGG'CCA
Cuts at: 2002 2002
Size: 4241
MslI CAynn'nnrTG
Cuts at: 507 2230 2357 507
Size: 1723 127 2391
Fragments arranged by size:
2391 1723 127
MspA1I CmG'CkG
Cuts at: 141 162 172 1352 1666 2026 3039 3284 141
Size: 21 10 1180 314 360 1013 245 1098
Fragments arranged by size:
1180 1098 1013 360 314 245 21 10
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 4241
NarI GG'CG_CC
Cuts at: 1672 1920 1672
Size: 248 3993
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 4241
NspI r_CATG'y
Cuts at: 1030 1172 1294 1794 2701 1030
Size: 142 122 500 907 2570
Fragments arranged by size:
2570 907 500 142 122
PasI CC'CwG_GG
Cuts at: 396 396
Size: 4241
PciI A'CATG_T
Cuts at: 2697 2697
Size: 4241
PflMI CCAn_nnn'nTGG
Cuts at: 493 493
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 4209
PstI C_TGCA'G
Cuts at: 1288 1973 1288
Size: 685 3556
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 1666 2026 1666
Size: 360 3881
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 1255 1255
Size: 4241
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 1118 3758 1118
Size: 2640 1601
SexAI A'CCwGG_T
Cuts at: 486 486
Size: 4241
SfcI C'TryA_G
Cuts at: 33 1284 1969 2962 3153 33
Size: 1251 685 993 191 1121
Fragments arranged by size:
1251 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1673 1921 1673
Size: 248 3993
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 379 1194 1396 2311 2803 3065 379
Size: 815 202 915 492 262 1555
Fragments arranged by size:
1555 915 815 492 262 202
SpeI A'CTAG_T
Cuts at: 67 819 67
Size: 752 3489
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
SspI AAT'ATT
Cuts at: 717 717
Size: 4241
StuI AGG'CCT
Cuts at: 991 991
Size: 4241
StyI C'CwwG_G
Cuts at: 1375 1729 1375
Size: 354 3887
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1076 1110 1824 1957 2416 2625 1076
Size: 34 714 133 459 209 2692
Fragments arranged by size:
2692 714 459 209 133 34
TatI w'GTAC_w
Cuts at: 361 1085 1116 3756 361
Size: 724 31 2640 846
Fragments arranged by size:
2640 846 724 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3758 3790 3758
Size: 32 4209
Tth111I GACn'n_nGTC
Cuts at: 2038 2038
Size: 4241
XbaI T'CTAG_A
Cuts at: 1272 1272
Size: 4241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1736 1736
Size: 4241
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
XmnI GAAnn'nnTTC
Cuts at: 121 121
Size: 4241
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI