Restriction map for
pF1KB6574
(Circular) (Six-base) MAPSORT of: pF1KB6574.seq Check: 4155 from: 1 to: 4316
pF1KB6574 4316 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AatII G_ACGT'C
Cuts at: 571 571
Size: 4316
AccI GT'mk_AC
Cuts at: 779 1354 779
Size: 575 3741
Acc65I G'GTAC_C
Cuts at: 247 1332 247
Size: 1085 3231
AclI AA'CG_TT
Cuts at: 175 4156 175
Size: 3981 335
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 711 889 1105 2141 2573 3320 3588 711
Size: 178 216 1036 432 747 268 1439
Fragments arranged by size:
1439 1036 747 432 268 216 178
AflIII A'CryG_T
Cuts at: 1668 2772 3749 1668
Size: 1104 977 2235
Fragments arranged by size:
2235 1104 977
AgeI A'CCGG_T
Cuts at: 2704 2704
Size: 4316
AleI CACnn'nnGTG
Cuts at: 335 335
Size: 4316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 391 391
Size: 4316
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 842 874 842
Size: 32 4284
AlwNI CAG_nnn'CTG
Cuts at: 867 1083 1621 3188 867
Size: 216 538 1567 1995
Fragments arranged by size:
1995 1567 538 216
ApaLI G'TGCA_C
Cuts at: 3086 3086
Size: 4316
ApoI r'AATT_y
Cuts at: 1320 3669 3925 1320
Size: 2349 256 1711
Fragments arranged by size:
2349 1711 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4316
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
AvaI C'yCGr_G
Cuts at: 1336 1336
Size: 4316
BamHI G'GATC_C
Cuts at: 1341 1341
Size: 4316
BanI G'GyrC_C
Cuts at: 247 793 1332 1746 1994 2029 247
Size: 546 539 414 248 35 2534
Fragments arranged by size:
2534 546 539 414 248 35
BanII G_rGCy'C
Cuts at: 1330 2360 1330
Size: 1030 3286
BbeI G_GCGC'C
Cuts at: 1750 1998 1750
Size: 248 4068
BbsI GAAGACnn'nnnn_
Cuts at: 289 1043 1213 289
Size: 754 170 3392
Fragments arranged by size:
3392 754 170
BciVI GTATCCnnnnn_n'
Cuts at: 2214 2975 4316 2214
Size: 761 1341 2214
Fragments arranged by size:
2214 1341 761
BclI T'GATC_A
Cuts at: 1836 1836
Size: 4316
BglI GCCn_nnn'nGGC
Cuts at: 762 762
Size: 4316
BglII A'GATC_T
Cuts at: 1831 3868 1831
Size: 2037 2279
BlpI GC'TnA_GC
Cuts at: 1428 1428
Size: 4316
Bme1580I G_kGCm'C
Cuts at: 1941 2034 3090 1941
Size: 93 1056 3167
Fragments arranged by size:
3167 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1064 1528 1609 1944 4088 1064
Size: 464 81 335 2144 1292
Fragments arranged by size:
2144 1292 464 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 497 2683 497
Size: 2186 2130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 1492 2371 2863 3161 466
Size: 1026 879 492 298 1621
Fragments arranged by size:
1621 1026 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 622 4020 622
Size: 3398 918
BsaAI yAC'GTr
Cuts at: 1669 1669
Size: 4316
BsaBI GATnn'nnATC
Cuts at: 6 1830 3867 6
Size: 1824 2037 455
Fragments arranged by size:
2037 1824 455
BsaHI Gr'CG_yC
Cuts at: 568 1747 1995 2697 4193 568
Size: 1179 248 702 1496 691
Fragments arranged by size:
1496 1179 702 691 248
BsaWI w'CCGG_w
Cuts at: 664 1505 2026 2704 2978 3125 3536 664
Size: 841 521 678 274 147 411 1444
Fragments arranged by size:
1444 841 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 165 195 364 394 165
Size: 30 169 30 4087
Fragments arranged by size:
4087 169 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 730 730
Size: 4316
BseYI C'CCAG_C
Cuts at: 1741 2499 3076 3572 1741
Size: 758 577 496 2485
Fragments arranged by size:
2485 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 970
Size: 4316
BsiEI CG_ry'CG
Cuts at: 104 1858 1904 3112 104
Size: 1754 46 1208 1308
Fragments arranged by size:
1754 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1330 2108 2298 3090 1330
Size: 778 190 792 2556
Fragments arranged by size:
2556 792 778 190
BsmI GAATG_Cn'
Cuts at: 430 721 430
Size: 291 4025
BsmBI CGTCTCn'nnnn_
Cuts at: 559 559
Size: 4316
Bsp1286I G_dGCh'C
Cuts at: 1330 1941 2034 2108 2298 2360 3090 1330
Size: 611 93 74 190 62 730 2556
Fragments arranged by size:
2556 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 664 3536 664
Size: 2872 1444
BspHI T'CATG_A
Cuts at: 3492 3492
Size: 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1366 1580 1882 2263 1366
Size: 214 302 381 3419
Fragments arranged by size:
3419 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2608 2662 4146 4311 50
Size: 2558 54 1484 165 55
Fragments arranged by size:
2558 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2228 2228
Size: 4316
BsrFI r'CCGG_y
Cuts at: 2314 2495 2704 2314
Size: 181 209 3926
Fragments arranged by size:
3926 209 181
BsrGI T'GTAC_A
Cuts at: 268 1238 268
Size: 970 3346
BssHII G'CGCG_C
Cuts at: 128 128
Size: 4316
BssSI C'ACGA_G
Cuts at: 133 2587 2945 133
Size: 2454 358 1504
Fragments arranged by size:
2454 1504 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1693 1693
Size: 4316
BstBI TT'CG_AA
Cuts at: 2677 2677
Size: 4316
BstEII G'GTnAC_C
Cuts at: 1276 1276
Size: 4316
BstYI r'GATC_y
Cuts at: 1 1079 1341 1808 1831 2166 2412 3424 3510
Size: 1078 262 467 23 335 246 1012 86
Cuts at: 3510 3522 3868 1
Size: 12 346 449
Fragments arranged by size:
1078 1012 467 449 346 335 262 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2266 2447 2610 2266
Size: 181 163 3972
Fragments arranged by size:
3972 181 163
BtsI GCAGTG_nn'
Cuts at: 612 1559 612
Size: 947 3369
DraI TTT'AAA
Cuts at: 1315 1315
Size: 4316
DrdI GACnn_nn'nnGTC
Cuts at: 2022 2880 2022
Size: 858 3458
EaeI y'GGCC_r
Cuts at: 1901 2075 2466 2493 3838 1901
Size: 174 391 27 1345 2379
Fragments arranged by size:
2379 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1901 1901
Size: 4316
EarI CTCTTCn'nnn_
Cuts at: 2339 2549 2339
Size: 210 4106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2524 2834 2980 4126 2524
Size: 310 146 1146 2714
Fragments arranged by size:
2714 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1328 1328
Size: 4316
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 711 889 1105 2141 2573 3320 3588 711
Size: 178 216 1036 432 747 268 1439
Fragments arranged by size:
1439 1036 747 432 268 216 178
EcoNI CCTnn'n_nnAGG
Cuts at: 712 712
Size: 4316
EcoO109I rG'GnC_Cy
Cuts at: 1137 1455 1137
Size: 318 3998
EcoRI G'AATT_C
Cuts at: 1320 1320
Size: 4316
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 4316
FspI TGC'GCA
Cuts at: 2097 2097
Size: 4316
HaeII r_GCGC'y
Cuts at: 441 1750 1998 3020 3999 441
Size: 1309 248 1022 979 758
Fragments arranged by size:
1309 1022 979 758 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 556 588 2158 2188 2190 2220 556
Size: 32 1570 30 2 30 2652
Fragments arranged by size:
2652 1570 32 30 30 2
HincII GTy'rAC
Cuts at: 1355 1355
Size: 4316
HindIII A'AGCT_T
Cuts at: 1008 1008
Size: 4316
KasI G'GCGC_C
Cuts at: 1746 1994 1746
Size: 248 4068
KpnI G_GTAC'C
Cuts at: 251 1336 251
Size: 1085 3231
MluI A'CGCG_T
Cuts at: 3749 3749
Size: 4316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2987 3171 2987
Size: 184 4132
MscI TGG'CCA
Cuts at: 2077 2077
Size: 4316
MslI CAynn'nnrTG
Cuts at: 335 2305 2432 335
Size: 1970 127 2219
Fragments arranged by size:
2219 1970 127
MspA1I CmG'CkG
Cuts at: 222 326 864 954 1427 1741 2101 3114 3359
Size: 104 538 90 473 314 360 1013 245
Cuts at: 3359 222
Size: 1179
Fragments arranged by size:
1179 1013 538 473 360 314 245 104
90
NaeI GCC'GGC
Cuts at: 2497 2497
Size: 4316
NarI GG'CG_CC
Cuts at: 1747 1995 1747
Size: 248 4068
NdeI CA'TA_TG
Cuts at: 658 658
Size: 4316
NgoMIV G'CCGG_C
Cuts at: 2495 2495
Size: 4316
NspI r_CATG'y
Cuts at: 970 1064 1369 1869 2776 970
Size: 94 305 500 907 2510
Fragments arranged by size:
2510 907 500 305 94
PciI A'CATG_T
Cuts at: 2772 2772
Size: 4316
PflMI CCAn_nnn'nTGG
Cuts at: 158 158
Size: 4316
PfoI T'CCnGG_A
Cuts at: 483 483
Size: 4316
PmeI GTTT'AAAC
Cuts at: 1315 1315
Size: 4316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 439 471 3455 3487 439
Size: 32 2984 32 1268
Fragments arranged by size:
2984 1268 32 32
PshAI GACnn'nnGTC
Cuts at: 236 344 236
Size: 108 4208
PstI C_TGCA'G
Cuts at: 358 380 1363 2048 358
Size: 22 983 685 2626
Fragments arranged by size:
2626 983 685 22
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4316
PvuII CAG'CTG
Cuts at: 222 326 864 1741 2101 222
Size: 104 538 877 360 2437
Fragments arranged by size:
2437 877 538 360 104
RsrII CG'GwC_CG
Cuts at: 2511 2511
Size: 4316
SacI G_AGCT'C
Cuts at: 1330 1330
Size: 4316
SalI G'TCGA_C
Cuts at: 1353 1353
Size: 4316
SapI GCTCTTCn'nnn_
Cuts at: 2339 2549 2339
Size: 210 4106
SbfI CC_TGCA'GG
Cuts at: 1363 1363
Size: 4316
ScaI AGT'ACT
Cuts at: 3833 3833
Size: 4316
SexAI A'CCwGG_T
Cuts at: 1280 1280
Size: 4316
SfcI C'TryA_G
Cuts at: 33 354 376 585 1359 2044 3037 3228 33
Size: 321 22 209 774 685 993 191 1121
Fragments arranged by size:
1121 993 774 685 321 209 191 22
SfoI GGC'GCC
Cuts at: 1748 1996 1748
Size: 248 4068
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4316
SmaI CCC'GGG
Cuts at: 1338 1338
Size: 4316
SmlI C'TyrA_G
Cuts at: 445 1471 2386 2878 3140 445
Size: 1026 915 492 262 1621
Fragments arranged by size:
1621 1026 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4316
SphI G_CATG'C
Cuts at: 1064 1369 1869 1064
Size: 305 500 3511
Fragments arranged by size:
3511 500 305
StyI C'CwwG_G
Cuts at: 1450 1804 1450
Size: 354 3962
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 181 421 1899 2032 2491 2700 181
Size: 240 1478 133 459 209 1797
Fragments arranged by size:
1797 1478 459 240 209 133
TatI w'GTAC_w
Cuts at: 268 1238 3831 268
Size: 970 2593 753
Fragments arranged by size:
2593 970 753
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 479 511 1010 1042 3833 3865 479
Size: 32 499 32 2791 32 930
Fragments arranged by size:
2791 930 499 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2113 2113
Size: 4316
XbaI T'CTAG_A
Cuts at: 1347 1347
Size: 4316
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1811 1811
Size: 4316
XmaI C'CCGG_G
Cuts at: 1336 1336
Size: 4316
XmnI GAAnn'nnTTC
Cuts at: 176 176
Size: 4316
ZraI GAC'GTC
Cuts at: 569 569
Size: 4316
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BpmI Bpu10I BsiWI
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI