Restriction map for
pF1KB6563
(Circular) (Six-base) MAPSORT of: pF1KB6563.seq Check: 2234 from: 1 to: 4235
pF1KB6563 4235 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 581 581
Size: 4235
AatII G_ACGT'C
Cuts at: 734 734
Size: 4235
AccI GT'mk_AC
Cuts at: 353 1273 353
Size: 920 3315
Acc65I G'GTAC_C
Cuts at: 1251 1251
Size: 4235
AclI AA'CG_TT
Cuts at: 4075 4075
Size: 4235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 1110 2060 2492 3239 3507 256
Size: 854 950 432 747 268 984
Fragments arranged by size:
984 950 854 747 432 268
AfeI AGC'GCT
Cuts at: 496 496
Size: 4235
AflIII A'CryG_T
Cuts at: 1049 1138 1587 2691 3668 1049
Size: 89 449 1104 977 1616
Fragments arranged by size:
1616 1104 977 449 89
AgeI A'CCGG_T
Cuts at: 2623 2623
Size: 4235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 682 682
Size: 4235
AlwNI CAG_nnn'CTG
Cuts at: 519 831 1540 3107 519
Size: 312 709 1567 1647
Fragments arranged by size:
1647 1567 709 312
ApaI G_GGCC'C
Cuts at: 256 1009 256
Size: 753 3482
ApaLI G'TGCA_C
Cuts at: 3005 3005
Size: 4235
ApoI r'AATT_y
Cuts at: 565 1239 3588 3844 565
Size: 674 2349 256 956
Fragments arranged by size:
2349 956 674 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4235
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
AvaI C'yCGr_G
Cuts at: 173 427 1087 1255 173
Size: 254 660 168 3153
Fragments arranged by size:
3153 660 254 168
BamHI G'GATC_C
Cuts at: 197 764 1260 197
Size: 567 496 3172
Fragments arranged by size:
3172 567 496
BanI G'GyrC_C
Cuts at: 143 307 1251 1665 1913 1948 143
Size: 164 944 414 248 35 2430
Fragments arranged by size:
2430 944 414 248 164 35
BanII G_rGCy'C
Cuts at: 256 1009 1249 2279 256
Size: 753 240 1030 2212
Fragments arranged by size:
2212 1030 753 240
BbeI G_GCGC'C
Cuts at: 311 1669 1917 311
Size: 1358 248 2629
Fragments arranged by size:
2629 1358 248
BbsI GAAGACnn'nnnn_
Cuts at: 1164 1164
Size: 4235
BbvCI CC'TCA_GC
Cuts at: 1022 1022
Size: 4235
BciVI GTATCCnnnnn_n'
Cuts at: 2133 2894 4235 2133
Size: 761 1341 2133
Fragments arranged by size:
2133 1341 761
BclI T'GATC_A
Cuts at: 1755 1755
Size: 4235
BglI GCCn_nnn'nGGC
Cuts at: 1091 1091
Size: 4235
BglII A'GATC_T
Cuts at: 1750 3787 1750
Size: 2037 2198
BlpI GC'TnA_GC
Cuts at: 1347 1347
Size: 4235
Bme1580I G_kGCm'C
Cuts at: 256 1009 1860 1953 3009 256
Size: 753 851 93 1056 1482
Fragments arranged by size:
1482 1056 851 753 93
BmgBI CAC'GTC
Cuts at: 651 1182 651
Size: 531 3704
BmrI ACTGGGnnnn_n'
Cuts at: 989 1447 1528 1863 4007 989
Size: 458 81 335 2144 1217
Fragments arranged by size:
2144 1217 458 335 81
BmtI G_CTAG'C
Cuts at: 449 449
Size: 4235
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 4235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 853 973 628
Size: 225 120 3890
Fragments arranged by size:
3890 225 120
Bpu10I CC'TnA_GC
Cuts at: 644 1010 1022 644
Size: 366 12 3857
Fragments arranged by size:
3857 366 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1411 2290 2782 3080 1411
Size: 879 492 298 2566
Fragments arranged by size:
2566 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3939 3939
Size: 4235
BsaAI yAC'GTr
Cuts at: 1588 1588
Size: 4235
BsaBI GATnn'nnATC
Cuts at: 6 295 1749 3786 6
Size: 289 1454 2037 455
Fragments arranged by size:
2037 1454 455 289
BsaHI Gr'CG_yC
Cuts at: 308 731 1119 1666 1914 2616 4112 308
Size: 423 388 547 248 702 1496 431
Fragments arranged by size:
1496 702 547 431 423 388 248
BsaWI w'CCGG_w
Cuts at: 1424 1945 2623 2897 3044 3455 1424
Size: 521 678 274 147 411 2204
Fragments arranged by size:
2204 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 682 712 682
Size: 30 4205
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 337 340 637 850 970 337
Size: 3 297 213 120 3602
Fragments arranged by size:
3602 297 213 120 3
BseYI C'CCAG_C
Cuts at: 1660 2418 2995 3491 1660
Size: 758 577 496 2404
Fragments arranged by size:
2404 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 724
Size: 4235
BsiEI CG_ry'CG
Cuts at: 104 1777 1823 3031 104
Size: 1673 46 1208 1308
Fragments arranged by size:
1673 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 277 656 1199 1249 2027 2217 3009 277
Size: 379 543 50 778 190 792 1503
Fragments arranged by size:
1503 792 778 543 379 190 50
Bsp1286I G_dGCh'C
Cuts at: 256 277 656 1009 1199 1249 1860 1953 2027
Size: 21 379 353 190 50 611 93 74
Cuts at: 2027 2217 2279 3009 256
Size: 190 62 730 1482
Fragments arranged by size:
1482 730 611 379 353 190 190 93
74 62 50 21
BspEI T'CCGG_A
Cuts at: 3455 3455
Size: 4235
BspHI T'CATG_A
Cuts at: 455 3411 455
Size: 2956 1279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 581 725 1232 1285 1499 1801 2182 581
Size: 144 507 53 214 302 381 2634
Fragments arranged by size:
2634 507 381 302 214 144 53
BsrBI CCG'CTC
Cuts at: 50 116 266 402 2527 2581 4065 4230 50
Size: 66 150 136 2125 54 1484 165 55
Fragments arranged by size:
2125 1484 165 150 136 66 55 54
BsrDI GCAATG_nn'
Cuts at: 2147 2147
Size: 4235
BsrFI r'CCGG_y
Cuts at: 310 532 2233 2414 2623 310
Size: 222 1701 181 209 1922
Fragments arranged by size:
1922 1701 222 209 181
BsrGI T'GTAC_A
Cuts at: 1052 1064 1052
Size: 12 4223
BssSI C'ACGA_G
Cuts at: 1056 2506 2864 1056
Size: 1450 358 2427
Fragments arranged by size:
2427 1450 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1612 1612
Size: 4235
BstBI TT'CG_AA
Cuts at: 2596 2596
Size: 4235
BstYI r'GATC_y
Cuts at: 1 197 764 1260 1727 1750 2085 2331 3343
Size: 196 567 496 467 23 335 246 1012
Cuts at: 3343 3429 3441 3787 1
Size: 86 12 346 449
Fragments arranged by size:
1012 567 496 467 449 346 335 246
196 86 23 12
BtgI C'CryG_G
Cuts at: 107 225 303 395 107
Size: 118 78 92 3947
Fragments arranged by size:
3947 118 92 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2185 2366 2529 2185
Size: 181 163 3891
Fragments arranged by size:
3891 181 163
BtsI GCAGTG_nn'
Cuts at: 520 1478 520
Size: 958 3277
DraI TTT'AAA
Cuts at: 1234 1234
Size: 4235
DraIII CAC_nnn'GTG
Cuts at: 814 1073 814
Size: 259 3976
DrdI GACnn_nn'nnGTC
Cuts at: 1941 2799 1941
Size: 858 3377
EaeI y'GGCC_r
Cuts at: 506 1820 1994 2385 2412 3757 506
Size: 1314 174 391 27 1345 984
Fragments arranged by size:
1345 1314 984 391 174 27
EagI C'GGCC_G
Cuts at: 1820 1820
Size: 4235
EarI CTCTTCn'nnn_
Cuts at: 265 1129 2258 2468 265
Size: 864 1129 210 2032
Fragments arranged by size:
2032 1129 864 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2443 2753 2899 4045 2443
Size: 310 146 1146 2633
Fragments arranged by size:
2633 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1247 1247
Size: 4235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 628 853 973 1110 2060 2492 3239 3507
Size: 372 225 120 137 950 432 747 268
Cuts at: 3507 256
Size: 984
Fragments arranged by size:
984 950 747 432 372 268 225 137
120
EcoNI CCTnn'n_nnAGG
Cuts at: 151 151
Size: 4235
EcoO109I rG'GnC_Cy
Cuts at: 465 1005 1006 1374 465
Size: 540 1 368 3326
Fragments arranged by size:
3326 540 368 1
EcoRI G'AATT_C
Cuts at: 565 1239 565
Size: 674 3561
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 294 2254 294
Size: 1960 2275
FspI TGC'GCA
Cuts at: 2016 2016
Size: 4235
HaeII r_GCGC'y
Cuts at: 132 311 498 1669 1917 2939 3918 132
Size: 179 187 1171 248 1022 979 449
Fragments arranged by size:
1171 1022 979 449 248 187 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2077 2107 2109 2139 2077
Size: 30 2 30 4173
Fragments arranged by size:
4173 30 30 2
HincII GTy'rAC
Cuts at: 1274 1274
Size: 4235
KasI G'GCGC_C
Cuts at: 307 1665 1913 307
Size: 1358 248 2629
Fragments arranged by size:
2629 1358 248
KpnI G_GTAC'C
Cuts at: 1255 1255
Size: 4235
MluI A'CGCG_T
Cuts at: 3668 3668
Size: 4235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2906 3090 2906
Size: 184 4051
MscI TGG'CCA
Cuts at: 1996 1996
Size: 4235
MslI CAynn'nnrTG
Cuts at: 367 1060 2224 2351 367
Size: 693 1164 127 2251
Fragments arranged by size:
2251 1164 693 127
MspA1I CmG'CkG
Cuts at: 227 1346 1660 2020 3033 3278 227
Size: 1119 314 360 1013 245 1184
Fragments arranged by size:
1184 1119 1013 360 314 245
NaeI GCC'GGC
Cuts at: 312 534 2416 312
Size: 222 1882 2131
Fragments arranged by size:
2131 1882 222
NarI GG'CG_CC
Cuts at: 308 1666 1914 308
Size: 1358 248 2629
Fragments arranged by size:
2629 1358 248
NcoI C'CATG_G
Cuts at: 107 395 107
Size: 288 3947
NgoMIV G'CCGG_C
Cuts at: 310 532 2414 310
Size: 222 1882 2131
Fragments arranged by size:
2131 1882 222
NheI G'CTAG_C
Cuts at: 445 445
Size: 4235
NspI r_CATG'y
Cuts at: 669 1053 1142 1288 1788 2695 669
Size: 384 89 146 500 907 2209
Fragments arranged by size:
2209 907 500 384 146 89
PciI A'CATG_T
Cuts at: 1049 1138 2691 1049
Size: 89 1553 2593
Fragments arranged by size:
2593 1553 89
PflMI CCAn_nnn'nTGG
Cuts at: 515 831 515
Size: 316 3919
PfoI T'CCnGG_A
Cuts at: 890 950 1043 890
Size: 60 93 4082
Fragments arranged by size:
4082 93 60
PmeI GTTT'AAAC
Cuts at: 1234 1234
Size: 4235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 4203
PpuMI rG'GwC_Cy
Cuts at: 465 465
Size: 4235
PspOMI G'GGCC_C
Cuts at: 252 1005 252
Size: 753 3482
PstI C_TGCA'G
Cuts at: 410 1282 1967 410
Size: 872 685 2678
Fragments arranged by size:
2678 872 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4235
PvuII CAG'CTG
Cuts at: 1660 2020 1660
Size: 360 3875
RsrII CG'GwC_CG
Cuts at: 2430 2430
Size: 4235
SacI G_AGCT'C
Cuts at: 1249 1249
Size: 4235
SacII CC_GC'GG
Cuts at: 228 228
Size: 4235
SalI G'TCGA_C
Cuts at: 1272 1272
Size: 4235
SapI GCTCTTCn'nnn_
Cuts at: 265 2258 2468 265
Size: 1993 210 2032
Fragments arranged by size:
2032 1993 210
SbfI CC_TGCA'GG
Cuts at: 1282 1282
Size: 4235
ScaI AGT'ACT
Cuts at: 691 3752 691
Size: 3061 1174
SfcI C'TryA_G
Cuts at: 33 406 1278 1963 2956 3147 33
Size: 373 872 685 993 191 1121
Fragments arranged by size:
1121 993 872 685 373 191
SfoI GGC'GCC
Cuts at: 309 1667 1915 309
Size: 1358 248 2629
Fragments arranged by size:
2629 1358 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
SmaI CCC'GGG
Cuts at: 175 1257 175
Size: 1082 3153
SmlI C'TyrA_G
Cuts at: 1390 2305 2797 3059 1390
Size: 915 492 262 2566
Fragments arranged by size:
2566 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4235
SphI G_CATG'C
Cuts at: 669 1288 1788 669
Size: 619 500 3116
Fragments arranged by size:
3116 619 500
StyI C'CwwG_G
Cuts at: 107 395 1369 1723 107
Size: 288 974 354 2619
Fragments arranged by size:
2619 974 354 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1818 1951 2410 2619 1818
Size: 133 459 209 3434
Fragments arranged by size:
3434 459 209 133
TatI w'GTAC_w
Cuts at: 590 689 1052 1064 3750 590
Size: 99 363 12 2686 1075
Fragments arranged by size:
2686 1075 363 99 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1145 1177 3752 3784 1145
Size: 32 2575 32 1596
Fragments arranged by size:
2575 1596 32 32
Tth111I GACn'n_nGTC
Cuts at: 2032 2032
Size: 4235
XbaI T'CTAG_A
Cuts at: 1266 1266
Size: 4235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 975 1730 975
Size: 755 3480
XmaI C'CCGG_G
Cuts at: 173 1255 173
Size: 1082 3153
XmnI GAAnn'nnTTC
Cuts at: 364 364
Size: 4235
ZraI GAC'GTC
Cuts at: 732 732
Size: 4235
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AleI AloI AscI AvrII BaeI BcgI
BfrBI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI