Restriction map for
pF1KB6560
(Circular) (Six-base) MAPSORT of: pF1KB6560.seq Check: 6295 from: 1 to: 4139
pF1KB6560 4139 bp
With 182 enzymes: SgfI *
July 28, 2009 13:24 ..
AccI GT'mk_AC
Cuts at: 1177 1177
Size: 4139
Acc65I G'GTAC_C
Cuts at: 1155 1155
Size: 4139
AclI AA'CG_TT
Cuts at: 3979 3979
Size: 4139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1964 2396 3143 3411 1964
Size: 432 747 268 2692
Fragments arranged by size:
2692 747 432 268
AfeI AGC'GCT
Cuts at: 222 222
Size: 4139
AflIII A'CryG_T
Cuts at: 177 243 1491 2595 3572 177
Size: 66 1248 1104 977 744
Fragments arranged by size:
1248 1104 977 744 66
AgeI A'CCGG_T
Cuts at: 2527 2527
Size: 4139
AleI CACnn'nnGTG
Cuts at: 182 182
Size: 4139
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 593 625 593
Size: 32 4107
AlwNI CAG_nnn'CTG
Cuts at: 1126 1444 3011 1126
Size: 318 1567 2254
Fragments arranged by size:
2254 1567 318
ApaI G_GGCC'C
Cuts at: 508 508
Size: 4139
ApaLI G'TGCA_C
Cuts at: 2909 2909
Size: 4139
ApoI r'AATT_y
Cuts at: 1143 3492 3748 1143
Size: 2349 256 1534
Fragments arranged by size:
2349 1534 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4139
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
AvaI C'yCGr_G
Cuts at: 1159 1159
Size: 4139
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 971 1004 971
Size: 33 4106
BamHI G'GATC_C
Cuts at: 1164 1164
Size: 4139
BanI G'GyrC_C
Cuts at: 228 435 1155 1569 1817 1852 228
Size: 207 720 414 248 35 2515
Fragments arranged by size:
2515 720 414 248 207 35
BanII G_rGCy'C
Cuts at: 326 508 559 1016 1153 2183 326
Size: 182 51 457 137 1030 2282
Fragments arranged by size:
2282 1030 457 182 137 51
BbeI G_GCGC'C
Cuts at: 1573 1821 1573
Size: 248 3891
BciVI GTATCCnnnnn_n'
Cuts at: 2037 2798 4139 2037
Size: 761 1341 2037
Fragments arranged by size:
2037 1341 761
BclI T'GATC_A
Cuts at: 455 1659 455
Size: 1204 2935
BglII A'GATC_T
Cuts at: 1654 3691 1654
Size: 2037 2102
BlpI GC'TnA_GC
Cuts at: 1010 1251 1010
Size: 241 3898
Bme1580I G_kGCm'C
Cuts at: 177 508 623 1764 1857 2913 177
Size: 331 115 1141 93 1056 1403
Fragments arranged by size:
1403 1141 1056 331 115 93
BmrI ACTGGGnnnn_n'
Cuts at: 1351 1432 1767 3911 1351
Size: 81 335 2144 1579
Fragments arranged by size:
2144 1579 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4139
Bpu10I CC'TnA_GC
Cuts at: 320 320
Size: 4139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 1315 2194 2686 2984 218
Size: 1097 879 492 298 1373
Fragments arranged by size:
1373 1097 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 673 960 3843 673
Size: 287 2883 969
Fragments arranged by size:
2883 969 287
BsaAI yAC'GTr
Cuts at: 180 246 1492 180
Size: 66 1246 2827
Fragments arranged by size:
2827 1246 66
BsaBI GATnn'nnATC
Cuts at: 6 1653 3690 6
Size: 1647 2037 455
Fragments arranged by size:
2037 1647 455
BsaHI Gr'CG_yC
Cuts at: 1570 1818 2520 4016 1570
Size: 248 702 1496 1693
Fragments arranged by size:
1693 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1328 1849 2527 2801 2948 3359 1328
Size: 521 678 274 147 411 2108
Fragments arranged by size:
2108 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 230 260 400 430 515 545 593 623 230
Size: 30 140 30 85 30 48 30 3746
Fragments arranged by size:
3746 140 85 48 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 972 972
Size: 4139
BseYI C'CCAG_C
Cuts at: 217 372 439 622 1097 1564 2322 2899 3395
Size: 155 67 183 475 467 758 577 496
Cuts at: 3395 217
Size: 961
Fragments arranged by size:
961 758 577 496 475 467 183 155
67
BsiEI CG_ry'CG
Cuts at: 104 502 1681 1727 2935 104
Size: 398 1179 46 1208 1308
Fragments arranged by size:
1308 1208 1179 398 46
BsiHKAI G_wGCw'C
Cuts at: 326 559 1043 1153 1931 2121 2913 326
Size: 233 484 110 778 190 792 1552
Fragments arranged by size:
1552 792 778 484 233 190 110
Bsp1286I G_dGCh'C
Cuts at: 177 326 508 559 623 1016 1043 1153 1764
Size: 149 182 51 64 393 27 110 611
Cuts at: 1764 1857 1931 2121 2183 2913 177
Size: 93 74 190 62 730 1403
Fragments arranged by size:
1403 730 611 393 190 182 149 110
93 74 64 62 51 27
BspEI T'CCGG_A
Cuts at: 3359 3359
Size: 4139
BspHI T'CATG_A
Cuts at: 3315 3315
Size: 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1189 1403 1705 2086 1189
Size: 214 302 381 3242
Fragments arranged by size:
3242 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2431 2485 3969 4134 50
Size: 2381 54 1484 165 55
Fragments arranged by size:
2381 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2051 2051
Size: 4139
BsrFI r'CCGG_y
Cuts at: 2137 2318 2527 2137
Size: 181 209 3749
Fragments arranged by size:
3749 209 181
BsrGI T'GTAC_A
Cuts at: 293 293
Size: 4139
BssSI C'ACGA_G
Cuts at: 2410 2768 2410
Size: 358 3781
BstAPI GCAn_nnn'nTGC
Cuts at: 1516 1516
Size: 4139
BstBI TT'CG_AA
Cuts at: 2500 2500
Size: 4139
BstXI CCAn_nnnn'nTGG
Cuts at: 225 225
Size: 4139
BstYI r'GATC_y
Cuts at: 1 709 1063 1164 1631 1654 1989 2235 3247
Size: 708 354 101 467 23 335 246 1012
Cuts at: 3247 3333 3345 3691 1
Size: 86 12 346 449
Fragments arranged by size:
1012 708 467 449 354 346 335 246
101 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 887 887
Size: 4139
BtgI C'CryG_G
Cuts at: 122 509 672 705 122
Size: 387 163 33 3556
Fragments arranged by size:
3556 387 163 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2089 2270 2433 2089
Size: 181 163 3795
Fragments arranged by size:
3795 181 163
BtsI GCAGTG_nn'
Cuts at: 623 742 915 1382 623
Size: 119 173 467 3380
Fragments arranged by size:
3380 467 173 119
DraI TTT'AAA
Cuts at: 1138 1138
Size: 4139
DraIII CAC_nnn'GTG
Cuts at: 428 428
Size: 4139
DrdI GACnn_nn'nnGTC
Cuts at: 1845 2703 1845
Size: 858 3281
EaeI y'GGCC_r
Cuts at: 499 1724 1898 2289 2316 3661 499
Size: 1225 174 391 27 1345 977
Fragments arranged by size:
1345 1225 977 391 174 27
EagI C'GGCC_G
Cuts at: 499 1724 499
Size: 1225 2914
EarI CTCTTCn'nnn_
Cuts at: 257 2162 2372 257
Size: 1905 210 2024
Fragments arranged by size:
2024 1905 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2347 2657 2803 3949 2347
Size: 310 146 1146 2537
Fragments arranged by size:
2537 1146 310 146
EcoICRI GAG'CTC
Cuts at: 324 557 1151 324
Size: 233 594 3312
Fragments arranged by size:
3312 594 233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1964 2396 3143 3411 1964
Size: 432 747 268 2692
Fragments arranged by size:
2692 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 236 236
Size: 4139
EcoO109I rG'GnC_Cy
Cuts at: 309 386 505 661 1278 309
Size: 77 119 156 617 3170
Fragments arranged by size:
3170 617 156 119 77
EcoRI G'AATT_C
Cuts at: 1143 1143
Size: 4139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 4139
FspI TGC'GCA
Cuts at: 1920 1920
Size: 4139
HaeII r_GCGC'y
Cuts at: 224 1573 1821 2843 3822 224
Size: 1349 248 1022 979 541
Fragments arranged by size:
1349 1022 979 541 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1981 2011 2013 2043 1981
Size: 30 2 30 4077
Fragments arranged by size:
4077 30 30 2
HincII GTy'rAC
Cuts at: 1178 1178
Size: 4139
HindIII A'AGCT_T
Cuts at: 801 801
Size: 4139
KasI G'GCGC_C
Cuts at: 1569 1817 1569
Size: 248 3891
KpnI G_GTAC'C
Cuts at: 1159 1159
Size: 4139
MluI A'CGCG_T
Cuts at: 3572 3572
Size: 4139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 890 2810 2994 890
Size: 1920 184 2035
Fragments arranged by size:
2035 1920 184
MscI TGG'CCA
Cuts at: 1900 1900
Size: 4139
MslI CAynn'nnrTG
Cuts at: 112 182 2128 2255 112
Size: 70 1946 127 1996
Fragments arranged by size:
1996 1946 127 70
MspA1I CmG'CkG
Cuts at: 1126 1250 1564 1924 2937 3182 1126
Size: 124 314 360 1013 245 2083
Fragments arranged by size:
2083 1013 360 314 245 124
NaeI GCC'GGC
Cuts at: 2320 2320
Size: 4139
NarI GG'CG_CC
Cuts at: 1570 1818 1570
Size: 248 3891
NcoI C'CATG_G
Cuts at: 705 705
Size: 4139
NgoMIV G'CCGG_C
Cuts at: 2318 2318
Size: 4139
NspI r_CATG'y
Cuts at: 143 1192 1692 2599 143
Size: 1049 500 907 1683
Fragments arranged by size:
1683 1049 907 500
PciI A'CATG_T
Cuts at: 2595 2595
Size: 4139
PflMI CCAn_nnn'nTGG
Cuts at: 428 1126 428
Size: 698 3441
PmeI GTTT'AAAC
Cuts at: 1138 1138
Size: 4139
PmlI CAC'GTG
Cuts at: 180 246 180
Size: 66 4073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 593 625 3278 3310 593
Size: 32 2653 32 1422
Fragments arranged by size:
2653 1422 32 32
PpuMI rG'GwC_Cy
Cuts at: 309 386 661 309
Size: 77 275 3787
Fragments arranged by size:
3787 275 77
PspOMI G'GGCC_C
Cuts at: 504 504
Size: 4139
PstI C_TGCA'G
Cuts at: 547 618 1186 1871 547
Size: 71 568 685 2815
Fragments arranged by size:
2815 685 568 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4139
PvuII CAG'CTG
Cuts at: 1126 1564 1924 1126
Size: 438 360 3341
Fragments arranged by size:
3341 438 360
RsrII CG'GwC_CG
Cuts at: 2334 2334
Size: 4139
SacI G_AGCT'C
Cuts at: 326 559 1153 326
Size: 233 594 3312
Fragments arranged by size:
3312 594 233
SalI G'TCGA_C
Cuts at: 1176 1176
Size: 4139
SanDI GG'GwC_CC
Cuts at: 309 309
Size: 4139
SapI GCTCTTCn'nnn_
Cuts at: 2162 2372 2162
Size: 210 3929
SbfI CC_TGCA'GG
Cuts at: 1186 1186
Size: 4139
ScaI AGT'ACT
Cuts at: 3656 3656
Size: 4139
SfcI C'TryA_G
Cuts at: 33 390 543 614 684 1182 1867 2860 3051
Size: 357 153 71 70 498 685 993 191
Cuts at: 3051 33
Size: 1121
Fragments arranged by size:
1121 993 685 498 357 191 153 71
70
SfoI GGC'GCC
Cuts at: 1571 1819 1571
Size: 248 3891
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
SmaI CCC'GGG
Cuts at: 1161 1161
Size: 4139
SmlI C'TyrA_G
Cuts at: 233 1294 2209 2701 2963 233
Size: 1061 915 492 262 1409
Fragments arranged by size:
1409 1061 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4139
SphI G_CATG'C
Cuts at: 1192 1692 1192
Size: 500 3639
SspI AAT'ATT
Cuts at: 338 338
Size: 4139
StuI AGG'CCT
Cuts at: 1054 1054
Size: 4139
StyI C'CwwG_G
Cuts at: 705 1273 1627 705
Size: 568 354 3217
Fragments arranged by size:
3217 568 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 135 172 453 685 804 1722 1855 2314 2523
Size: 37 281 232 119 918 133 459 209
Cuts at: 2523 135
Size: 1751
Fragments arranged by size:
1751 918 459 281 232 209 133 119
37
TatI w'GTAC_w
Cuts at: 293 3654 293
Size: 3361 778
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 230 262 400 432 515 547 3656 3688 230
Size: 32 138 32 83 32 3109 32 681
Fragments arranged by size:
3109 681 138 83 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 537 1936 537
Size: 1399 2740
XbaI T'CTAG_A
Cuts at: 1170 1170
Size: 4139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 447 1634 447
Size: 1187 2952
XmaI C'CCGG_G
Cuts at: 1159 1159
Size: 4139
XmnI GAAnn'nnTTC
Cuts at: 349 768 349
Size: 419 3720
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AscI AvrII BbsI
BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI