Restriction map for
pF1KB6552
(Circular) (Six-base) MAPSORT of: pF1KB6552.seq Check: 1427 from: 1 to: 4295
pF1KB6552 4295 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 428 1333 428
Size: 905 3390
Acc65I G'GTAC_C
Cuts at: 1311 1311
Size: 4295
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1053 1188 2120 2552 3299 3567 1053
Size: 135 932 432 747 268 1781
Fragments arranged by size:
1781 932 747 432 268 135
AflIII A'CryG_T
Cuts at: 1647 2751 3728 1647
Size: 1104 977 2214
Fragments arranged by size:
2214 1104 977
AgeI A'CCGG_T
Cuts at: 2683 2683
Size: 4295
AleI CACnn'nnGTG
Cuts at: 322 322
Size: 4295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 259 259
Size: 4295
AlwNI CAG_nnn'CTG
Cuts at: 366 498 662 878 1089 1110 1600 3167 366
Size: 132 164 216 211 21 490 1567 1494
Fragments arranged by size:
1567 1494 490 216 211 164 132 21
ApaLI G'TGCA_C
Cuts at: 940 1228 3065 940
Size: 288 1837 2170
Fragments arranged by size:
2170 1837 288
ApoI r'AATT_y
Cuts at: 574 1299 3648 3904 574
Size: 725 2349 256 965
Fragments arranged by size:
2349 965 725 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4295
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 1146 1315 1146
Size: 169 4126
AvrII C'CTAG_G
Cuts at: 291 291
Size: 4295
BamHI G'GATC_C
Cuts at: 1320 1320
Size: 4295
BanI G'GyrC_C
Cuts at: 211 1311 1725 1973 2008 211
Size: 1100 414 248 35 2498
Fragments arranged by size:
2498 1100 414 248 35
BanII G_rGCy'C
Cuts at: 346 641 1309 2339 346
Size: 295 668 1030 2302
Fragments arranged by size:
2302 1030 668 295
BbeI G_GCGC'C
Cuts at: 1729 1977 1729
Size: 248 4047
BbsI GAAGACnn'nnnn_
Cuts at: 1194 1194
Size: 4295
BciVI GTATCCnnnnn_n'
Cuts at: 2193 2954 4295 2193
Size: 761 1341 2193
Fragments arranged by size:
2193 1341 761
BclI T'GATC_A
Cuts at: 1815 1815
Size: 4295
BglI GCCn_nnn'nGGC
Cuts at: 953 953
Size: 4295
BglII A'GATC_T
Cuts at: 1810 3847 1810
Size: 2037 2258
BlpI GC'TnA_GC
Cuts at: 1407 1407
Size: 4295
Bme1580I G_kGCm'C
Cuts at: 944 1232 1920 2013 3069 944
Size: 288 688 93 1056 2170
Fragments arranged by size:
2170 1056 688 288 93
BmgBI CAC'GTC
Cuts at: 1118 1118
Size: 4295
BmrI ACTGGGnnnn_n'
Cuts at: 620 838 1507 1588 1923 4067 620
Size: 218 669 81 335 2144 848
Fragments arranged by size:
2144 848 669 335 218 81
BmtI G_CTAG'C
Cuts at: 1142 1142
Size: 4295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 4295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1036 376
Size: 660 3635
Bpu10I CC'TnA_GC
Cuts at: 338 338
Size: 4295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1471 2350 2842 3140 1471
Size: 879 492 298 2626
Fragments arranged by size:
2626 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1221 3999 1221
Size: 2778 1517
BsaAI yAC'GTr
Cuts at: 1648 1648
Size: 4295
BsaBI GATnn'nnATC
Cuts at: 6 1809 3846 6
Size: 1803 2037 455
Fragments arranged by size:
2037 1803 455
BsaHI Gr'CG_yC
Cuts at: 761 1726 1974 2676 4172 761
Size: 965 248 702 1496 884
Fragments arranged by size:
1496 965 884 702 248
BsaWI w'CCGG_w
Cuts at: 1212 1484 2005 2683 2957 3104 3515 1212
Size: 272 521 678 274 147 411 1992
Fragments arranged by size:
1992 678 521 411 274 272 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 859 889 1263 1293 859
Size: 30 374 30 3861
Fragments arranged by size:
3861 374 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 391 394 783 1033 136
Size: 255 3 389 250 3398
Fragments arranged by size:
3398 389 255 250 3
BseYI C'CCAG_C
Cuts at: 1240 1282 1720 2478 3055 3551 1240
Size: 42 438 758 577 496 1984
Fragments arranged by size:
1984 758 577 496 438 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 1243 511
Size: 732 3563
BsiEI CG_ry'CG
Cuts at: 104 967 1837 1883 3091 104
Size: 863 870 46 1208 1308
Fragments arranged by size:
1308 1208 870 863 46
BsiHKAI G_wGCw'C
Cuts at: 152 944 1232 1309 2087 2277 3069 152
Size: 792 288 77 778 190 792 1378
Fragments arranged by size:
1378 792 792 778 288 190 77
Bsp1286I G_dGCh'C
Cuts at: 152 346 641 944 1232 1309 1920 2013 2087
Size: 194 295 303 288 77 611 93 74
Cuts at: 2087 2277 2339 3069 152
Size: 190 62 730 1378
Fragments arranged by size:
1378 730 611 303 295 288 194 190
93 77 74 62
BspEI T'CCGG_A
Cuts at: 3515 3515
Size: 4295
BspHI T'CATG_A
Cuts at: 3471 3471
Size: 4295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1265 1345 1559 1861 2242 1265
Size: 80 214 302 381 3318
Fragments arranged by size:
3318 381 302 214 80
BsrBI CCG'CTC
Cuts at: 50 230 1164 2587 2641 4125 4290 50
Size: 180 934 1423 54 1484 165 55
Fragments arranged by size:
1484 1423 934 180 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2207 2207
Size: 4295
BsrFI r'CCGG_y
Cuts at: 2293 2474 2683 2293
Size: 181 209 3905
Fragments arranged by size:
3905 209 181
BssHII G'CGCG_C
Cuts at: 447 447
Size: 4295
BssSI C'ACGA_G
Cuts at: 2566 2924 2566
Size: 358 3937
BstAPI GCAn_nnn'nTGC
Cuts at: 366 498 1089 1672 366
Size: 132 591 583 2989
Fragments arranged by size:
2989 591 583 132
BstBI TT'CG_AA
Cuts at: 2656 2656
Size: 4295
BstXI CCAn_nnnn'nTGG
Cuts at: 597 981 597
Size: 384 3911
BstYI r'GATC_y
Cuts at: 1 362 701 1320 1787 1810 2145 2391 3403
Size: 361 339 619 467 23 335 246 1012
Cuts at: 3403 3489 3501 3847 1
Size: 86 12 346 449
Fragments arranged by size:
1012 619 467 449 361 346 339 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 207 924 1220 107
Size: 100 717 296 3182
Fragments arranged by size:
3182 717 296 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2245 2426 2589 2245
Size: 181 163 3951
Fragments arranged by size:
3951 181 163
BtsI GCAGTG_nn'
Cuts at: 150 732 942 1538 150
Size: 582 210 596 2907
Fragments arranged by size:
2907 596 582 210
ClaI AT'CG_AT
Cuts at: 905 905
Size: 4295
DraI TTT'AAA
Cuts at: 1294 1294
Size: 4295
DrdI GACnn_nn'nnGTC
Cuts at: 2001 2859 2001
Size: 858 3437
EaeI y'GGCC_r
Cuts at: 968 1112 1880 2054 2445 2472 3817 968
Size: 144 768 174 391 27 1345 1446
Fragments arranged by size:
1446 1345 768 391 174 144 27
EagI C'GGCC_G
Cuts at: 1880 1880
Size: 4295
EarI CTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2503 2813 2959 4105 2503
Size: 310 146 1146 2693
Fragments arranged by size:
2693 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1307 1307
Size: 4295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1036 1053 1188 2120 2552 3299 3567 376
Size: 660 17 135 932 432 747 268 1104
Fragments arranged by size:
1104 932 747 660 432 268 135 17
EcoNI CCTnn'n_nnAGG
Cuts at: 463 463
Size: 4295
EcoO109I rG'GnC_Cy
Cuts at: 594 668 691 1150 1434 594
Size: 74 23 459 284 3455
Fragments arranged by size:
3455 459 284 74 23
EcoRI G'AATT_C
Cuts at: 574 1299 574
Size: 725 3570
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 4295
FspI TGC'GCA
Cuts at: 2076 2076
Size: 4295
HaeII r_GCGC'y
Cuts at: 499 1047 1729 1977 2999 3978 499
Size: 548 682 248 1022 979 816
Fragments arranged by size:
1022 979 816 682 548 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 859 891 2137 2167 2169 2199 859
Size: 32 1246 30 2 30 2955
Fragments arranged by size:
2955 1246 32 30 30 2
HincII GTy'rAC
Cuts at: 303 1334 303
Size: 1031 3264
KasI G'GCGC_C
Cuts at: 1725 1973 1725
Size: 248 4047
KpnI G_GTAC'C
Cuts at: 1315 1315
Size: 4295
MluI A'CGCG_T
Cuts at: 3728 3728
Size: 4295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2966 3150 2966
Size: 184 4111
MscI TGG'CCA
Cuts at: 1114 2056 1114
Size: 942 3353
MslI CAynn'nnrTG
Cuts at: 322 844 2284 2411 322
Size: 522 1440 127 2206
Fragments arranged by size:
2206 1440 522 127
MspA1I CmG'CkG
Cuts at: 200 1086 1286 1406 1720 2080 3093 3338 200
Size: 886 200 120 314 360 1013 245 1157
Fragments arranged by size:
1157 1013 886 360 314 245 200 120
NaeI GCC'GGC
Cuts at: 2476 2476
Size: 4295
NarI GG'CG_CC
Cuts at: 1726 1974 1726
Size: 248 4047
NcoI C'CATG_G
Cuts at: 107 107
Size: 4295
NgoMIV G'CCGG_C
Cuts at: 2474 2474
Size: 4295
NheI G'CTAG_C
Cuts at: 1138 1138
Size: 4295
NspI r_CATG'y
Cuts at: 756 1348 1848 2755 756
Size: 592 500 907 2296
Fragments arranged by size:
2296 907 592 500
PasI CC'CwG_GG
Cuts at: 216 765 216
Size: 549 3746
PciI A'CATG_T
Cuts at: 2751 2751
Size: 4295
PflMI CCAn_nnn'nTGG
Cuts at: 1110 1110
Size: 4295
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 4263
PpuMI rG'GwC_Cy
Cuts at: 668 691 1150 668
Size: 23 459 3813
Fragments arranged by size:
3813 459 23
PstI C_TGCA'G
Cuts at: 145 259 658 1094 1342 2027 145
Size: 114 399 436 248 685 2413
Fragments arranged by size:
2413 685 436 399 248 114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 1086 1286 1720 2080 1086
Size: 200 434 360 3301
Fragments arranged by size:
3301 434 360 200
RsrII CG'GwC_CG
Cuts at: 2490 2490
Size: 4295
SacI G_AGCT'C
Cuts at: 1309 1309
Size: 4295
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SanDI GG'GwC_CC
Cuts at: 668 1150 668
Size: 482 3813
SapI GCTCTTCn'nnn_
Cuts at: 2318 2528 2318
Size: 210 4085
SbfI CC_TGCA'GG
Cuts at: 1342 1342
Size: 4295
ScaI AGT'ACT
Cuts at: 3812 3812
Size: 4295
SfcI C'TryA_G
Cuts at: 33 141 255 654 1090 1338 2023 3016 3207
Size: 108 114 399 436 248 685 993 191
Cuts at: 3207 33
Size: 1121
Fragments arranged by size:
1121 993 685 436 399 248 191 114
108
SfoI GGC'GCC
Cuts at: 1727 1975 1727
Size: 248 4047
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 1317 1317
Size: 4295
SmlI C'TyrA_G
Cuts at: 1450 2365 2857 3119 1450
Size: 915 492 262 2626
Fragments arranged by size:
2626 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 1348 1848 1348
Size: 500 3795
StuI AGG'CCT
Cuts at: 1024 1024
Size: 4295
StyI C'CwwG_G
Cuts at: 107 291 590 641 1429 1783 107
Size: 184 299 51 788 354 2619
Fragments arranged by size:
2619 788 354 299 184 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 288 1878 2011 2470 2679 288
Size: 1590 133 459 209 1904
Fragments arranged by size:
1904 1590 459 209 133
TatI w'GTAC_w
Cuts at: 830 899 3810 830
Size: 69 2911 1315
Fragments arranged by size:
2911 1315 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1263 1295 3812 3844 1263
Size: 32 2517 32 1714
Fragments arranged by size:
2517 1714 32 32
Tth111I GACn'n_nGTC
Cuts at: 2092 2092
Size: 4295
XbaI T'CTAG_A
Cuts at: 1326 1326
Size: 4295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 639 978 1790 114
Size: 525 339 812 2619
Fragments arranged by size:
2619 812 525 339
XmaI C'CCGG_G
Cuts at: 1315 1315
Size: 4295
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
BaeI BbvCI BcgI BfrBI BsiWI BsmI BsmBI BsrGI
BstEII BstZ17I Bsu36I CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI