Restriction map for
pF1KB6547
(Circular) (Six-base) MAPSORT of: pF1KB6547.seq Check: 5614 from: 1 to: 4223
pF1KB6547 4223 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 1261 1261
Size: 4223
Acc65I G'GTAC_C
Cuts at: 1239 1239
Size: 4223
AclI AA'CG_TT
Cuts at: 4063 4063
Size: 4223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 2048 2480 3227 3495 979
Size: 1069 432 747 268 1707
Fragments arranged by size:
1707 1069 747 432 268
AflII C'TTAA_G
Cuts at: 1096 1096
Size: 4223
AflIII A'CryG_T
Cuts at: 543 1575 2679 3656 543
Size: 1032 1104 977 1110
Fragments arranged by size:
1110 1104 1032 977
AgeI A'CCGG_T
Cuts at: 2611 2611
Size: 4223
AlwNI CAG_nnn'CTG
Cuts at: 1528 3095 1528
Size: 1567 2656
ApaLI G'TGCA_C
Cuts at: 254 2993 254
Size: 2739 1484
ApoI r'AATT_y
Cuts at: 246 343 412 426 761 1227 3576 3832 246
Size: 97 69 14 335 466 2349 256 637
Fragments arranged by size:
2349 637 466 335 256 97 69 14
AseI AT'TA_AT
Cuts at: 20 769 20
Size: 749 3474
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
AvaI C'yCGr_G
Cuts at: 155 1243 155
Size: 1088 3135
BamHI G'GATC_C
Cuts at: 1248 1248
Size: 4223
BanI G'GyrC_C
Cuts at: 1239 1653 1901 1936 1239
Size: 414 248 35 3526
Fragments arranged by size:
3526 414 248 35
BanII G_rGCy'C
Cuts at: 1050 1237 2267 1050
Size: 187 1030 3006
Fragments arranged by size:
3006 1030 187
BbeI G_GCGC'C
Cuts at: 1657 1905 1657
Size: 248 3975
BbsI GAAGACnn'nnnn_
Cuts at: 153 785 153
Size: 632 3591
BciVI GTATCCnnnnn_n'
Cuts at: 2121 2882 4223 2121
Size: 761 1341 2121
Fragments arranged by size:
2121 1341 761
BclI T'GATC_A
Cuts at: 1077 1743 1077
Size: 666 3557
BglII A'GATC_T
Cuts at: 1738 3775 1738
Size: 2037 2186
BlpI GC'TnA_GC
Cuts at: 1335 1335
Size: 4223
Bme1580I G_kGCm'C
Cuts at: 258 1848 1941 2997 258
Size: 1590 93 1056 1484
Fragments arranged by size:
1590 1484 1056 93
BmgBI CAC'GTC
Cuts at: 171 171
Size: 4223
BmrI ACTGGGnnnn_n'
Cuts at: 140 302 831 1435 1516 1851 3995 140
Size: 162 529 604 81 335 2144 368
Fragments arranged by size:
2144 604 529 368 335 162 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 4223
Bpu10I CC'TnA_GC
Cuts at: 1106 1106
Size: 4223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1034 1399 2278 2770 3068 1034
Size: 365 879 492 298 2189
Fragments arranged by size:
2189 879 492 365 298
BsaI GGTCTCn'nnnn_
Cuts at: 3927 3927
Size: 4223
BsaAI yAC'GTr
Cuts at: 864 1576 864
Size: 712 3511
BsaBI GATnn'nnATC
Cuts at: 6 1737 3774 6
Size: 1731 2037 455
Fragments arranged by size:
2037 1731 455
BsaHI Gr'CG_yC
Cuts at: 1654 1902 2604 4100 1654
Size: 248 702 1496 1777
Fragments arranged by size:
1777 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1412 1933 2611 2885 3032 3443 1412
Size: 521 678 274 147 411 2192
Fragments arranged by size:
2192 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 515 515
Size: 4223
BseYI C'CCAG_C
Cuts at: 1648 2406 2983 3479 1648
Size: 758 577 496 2392
Fragments arranged by size:
2392 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1765 1811 3019 104
Size: 1661 46 1208 1308
Fragments arranged by size:
1661 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 258 1050 1237 2015 2205 2997 258
Size: 792 187 778 190 792 1484
Fragments arranged by size:
1484 792 792 778 190 187
Bsp1286I G_dGCh'C
Cuts at: 258 1050 1237 1848 1941 2015 2205 2267 2997
Size: 792 187 611 93 74 190 62 730
Cuts at: 2997 258
Size: 1484
Fragments arranged by size:
1484 792 730 611 190 187 93 74
62
BspEI T'CCGG_A
Cuts at: 3443 3443
Size: 4223
BspHI T'CATG_A
Cuts at: 3399 3399
Size: 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 630 1273 1487 1789 2170 630
Size: 643 214 302 381 2683
Fragments arranged by size:
2683 643 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2515 2569 4053 4218 50
Size: 2465 54 1484 165 55
Fragments arranged by size:
2465 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2135 2135
Size: 4223
BsrFI r'CCGG_y
Cuts at: 2221 2402 2611 2221
Size: 181 209 3833
Fragments arranged by size:
3833 209 181
BsrGI T'GTAC_A
Cuts at: 262 262
Size: 4223
BssSI C'ACGA_G
Cuts at: 2494 2852 2494
Size: 358 3865
BstAPI GCAn_nnn'nTGC
Cuts at: 1201 1600 1201
Size: 399 3824
BstBI TT'CG_AA
Cuts at: 2584 2584
Size: 4223
BstYI r'GATC_y
Cuts at: 1 1248 1715 1738 2073 2319 3331 3417 3429
Size: 1247 467 23 335 246 1012 86 12
Cuts at: 3429 3775 1
Size: 346 449
Fragments arranged by size:
1247 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 725 725
Size: 4223
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2173 2354 2517 2173
Size: 181 163 3879
Fragments arranged by size:
3879 181 163
BtsI GCAGTG_nn'
Cuts at: 1466 1466
Size: 4223
DraI TTT'AAA
Cuts at: 746 1222 746
Size: 476 3747
DrdI GACnn_nn'nnGTC
Cuts at: 1929 2787 1929
Size: 858 3365
EaeI y'GGCC_r
Cuts at: 555 1808 1982 2373 2400 3745 555
Size: 1253 174 391 27 1345 1033
Fragments arranged by size:
1345 1253 1033 391 174 27
EagI C'GGCC_G
Cuts at: 1808 1808
Size: 4223
EarI CTCTTCn'nnn_
Cuts at: 2246 2456 2246
Size: 210 4013
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2431 2741 2887 4033 2431
Size: 310 146 1146 2621
Fragments arranged by size:
2621 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1048 1235 1048
Size: 187 4036
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 2048 2480 3227 3495 979
Size: 1069 432 747 268 1707
Fragments arranged by size:
1707 1069 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1362 1362
Size: 4223
EcoRI G'AATT_C
Cuts at: 1227 1227
Size: 4223
EcoRV GAT'ATC
Cuts at: 225 225
Size: 4223
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 4223
FspI TGC'GCA
Cuts at: 2004 2004
Size: 4223
HaeII r_GCGC'y
Cuts at: 1657 1905 2927 3906 1657
Size: 248 1022 979 1974
Fragments arranged by size:
1974 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2065 2095 2097 2127 2065
Size: 30 2 30 4161
Fragments arranged by size:
4161 30 30 2
HincII GTy'rAC
Cuts at: 1262 1262
Size: 4223
KasI G'GCGC_C
Cuts at: 1653 1901 1653
Size: 248 3975
KpnI G_GTAC'C
Cuts at: 1243 1243
Size: 4223
MfeI C'AATT_G
Cuts at: 983 983
Size: 4223
MluI A'CGCG_T
Cuts at: 3656 3656
Size: 4223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 164 544 2894 3078 164
Size: 380 2350 184 1309
Fragments arranged by size:
2350 1309 380 184
MscI TGG'CCA
Cuts at: 557 1984 557
Size: 1427 2796
MslI CAynn'nnrTG
Cuts at: 533 2212 2339 533
Size: 1679 127 2417
Fragments arranged by size:
2417 1679 127
MspA1I CmG'CkG
Cuts at: 1334 1648 2008 3021 3266 1334
Size: 314 360 1013 245 2291
Fragments arranged by size:
2291 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2404 2404
Size: 4223
NarI GG'CG_CC
Cuts at: 1654 1902 1654
Size: 248 3975
NgoMIV G'CCGG_C
Cuts at: 2402 2402
Size: 4223
NspI r_CATG'y
Cuts at: 547 1276 1776 2683 547
Size: 729 500 907 2087
Fragments arranged by size:
2087 907 729 500
PciI A'CATG_T
Cuts at: 543 2679 543
Size: 2136 2087
PflMI CCAn_nnn'nTGG
Cuts at: 694 694
Size: 4223
PmeI GTTT'AAAC
Cuts at: 1222 1222
Size: 4223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 4191
PspXI vC'TCGA_Gb
Cuts at: 155 155
Size: 4223
PstI C_TGCA'G
Cuts at: 1270 1955 1270
Size: 685 3538
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4223
PvuII CAG'CTG
Cuts at: 1648 2008 1648
Size: 360 3863
RsrII CG'GwC_CG
Cuts at: 2418 2418
Size: 4223
SacI G_AGCT'C
Cuts at: 1050 1237 1050
Size: 187 4036
SalI G'TCGA_C
Cuts at: 1260 1260
Size: 4223
SapI GCTCTTCn'nnn_
Cuts at: 2246 2456 2246
Size: 210 4013
SbfI CC_TGCA'GG
Cuts at: 1270 1270
Size: 4223
ScaI AGT'ACT
Cuts at: 3740 3740
Size: 4223
SfcI C'TryA_G
Cuts at: 33 917 1266 1951 2944 3135 33
Size: 884 349 685 993 191 1121
Fragments arranged by size:
1121 993 884 685 349 191
SfoI GGC'GCC
Cuts at: 1655 1903 1655
Size: 248 3975
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
SmaI CCC'GGG
Cuts at: 1245 1245
Size: 4223
SmlI C'TyrA_G
Cuts at: 155 1049 1096 1378 2293 2785 3047 155
Size: 894 47 282 915 492 262 1331
Fragments arranged by size:
1331 915 894 492 282 262 47
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4223
SphI G_CATG'C
Cuts at: 1276 1776 1276
Size: 500 3723
SspI AAT'ATT
Cuts at: 683 942 683
Size: 259 3964
StyI C'CwwG_G
Cuts at: 1357 1711 1357
Size: 354 3869
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1806 1939 2398 2607 1806
Size: 133 459 209 3422
Fragments arranged by size:
3422 459 209 133
TatI w'GTAC_w
Cuts at: 262 933 3738 262
Size: 671 2805 747
Fragments arranged by size:
2805 747 671
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 501 533 563 595 3740 3772 501
Size: 32 30 32 3145 32 952
Fragments arranged by size:
3145 952 32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 2020 2020
Size: 4223
XbaI T'CTAG_A
Cuts at: 1254 1254
Size: 4223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1718 1718
Size: 4223
XhoI C'TCGA_G
Cuts at: 155 155
Size: 4223
XmaI C'CCGG_G
Cuts at: 1243 1243
Size: 4223
XmnI GAAnn'nnTTC
Cuts at: 248 248
Size: 4223
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PmeI PpiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BpmI BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I BtgI ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI ZraI