Restriction map for
pF1KB6545
(Circular) (Six-base) MAPSORT of: pF1KB6545.seq Check: 7610 from: 1 to: 4208
pF1KB6545 4208 bp
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 1246 1246
Size: 4208
Acc65I G'GTAC_C
Cuts at: 1224 1224
Size: 4208
AclI AA'CG_TT
Cuts at: 4048 4048
Size: 4208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 2033 2465 3212 3480 202
Size: 1831 432 747 268 930
Fragments arranged by size:
1831 930 747 432 268
AflIII A'CryG_T
Cuts at: 1560 2664 3641 1560
Size: 1104 977 2127
Fragments arranged by size:
2127 1104 977
AgeI A'CCGG_T
Cuts at: 2596 2596
Size: 4208
AlwNI CAG_nnn'CTG
Cuts at: 1513 3080 1513
Size: 1567 2641
ApaLI G'TGCA_C
Cuts at: 2978 2978
Size: 4208
ApoI r'AATT_y
Cuts at: 763 889 1212 3561 3817 763
Size: 126 323 2349 256 1154
Fragments arranged by size:
2349 1154 323 256 126
AseI AT'TA_AT
Cuts at: 20 665 1048 20
Size: 645 383 3180
Fragments arranged by size:
3180 645 383
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
AvaI C'yCGr_G
Cuts at: 204 1228 204
Size: 1024 3184
BamHI G'GATC_C
Cuts at: 1072 1233 1072
Size: 161 4047
BanI G'GyrC_C
Cuts at: 820 1224 1638 1886 1921 820
Size: 404 414 248 35 3107
Fragments arranged by size:
3107 414 404 248 35
BanII G_rGCy'C
Cuts at: 1222 2252 1222
Size: 1030 3178
BbeI G_GCGC'C
Cuts at: 1642 1890 1642
Size: 248 3960
BbsI GAAGACnn'nnnn_
Cuts at: 373 995 373
Size: 622 3586
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4174
BciVI GTATCCnnnnn_n'
Cuts at: 2106 2867 4208 2106
Size: 761 1341 2106
Fragments arranged by size:
2106 1341 761
BclI T'GATC_A
Cuts at: 788 1728 788
Size: 940 3268
BglII A'GATC_T
Cuts at: 1723 3760 1723
Size: 2037 2171
BlpI GC'TnA_GC
Cuts at: 398 1320 398
Size: 922 3286
Bme1580I G_kGCm'C
Cuts at: 1833 1926 2982 1833
Size: 93 1056 3059
Fragments arranged by size:
3059 1056 93
BmgBI CAC'GTC
Cuts at: 1016 1016
Size: 4208
BmrI ACTGGGnnnn_n'
Cuts at: 993 1420 1501 1836 3980 993
Size: 427 81 335 2144 1221
Fragments arranged by size:
2144 1221 427 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 339 2575 339
Size: 2236 1972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 555 983 1201 448
Size: 107 428 218 3455
Fragments arranged by size:
3455 428 218 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 396 1384 2263 2755 3053 133
Size: 263 988 879 492 298 1288
Fragments arranged by size:
1288 988 879 492 298 263
BsaI GGTCTCn'nnnn_
Cuts at: 573 868 3912 573
Size: 295 3044 869
Fragments arranged by size:
3044 869 295
BsaAI yAC'GTr
Cuts at: 1561 1561
Size: 4208
BsaBI GATnn'nnATC
Cuts at: 6 1722 3759 6
Size: 1716 2037 455
Fragments arranged by size:
2037 1716 455
BsaHI Gr'CG_yC
Cuts at: 1639 1887 2589 4085 1639
Size: 248 702 1496 1762
Fragments arranged by size:
1762 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1397 1918 2596 2870 3017 3428 1397
Size: 521 678 274 147 411 2177
Fragments arranged by size:
2177 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 853 883 853
Size: 30 4178
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 136 667 861 127
Size: 9 531 194 3474
Fragments arranged by size:
3474 531 194 9
BseYI C'CCAG_C
Cuts at: 111 710 1633 2391 2968 3464 111
Size: 599 923 758 577 496 855
Fragments arranged by size:
923 855 758 599 577 496
BsiEI CG_ry'CG
Cuts at: 104 1750 1796 3004 104
Size: 1646 46 1208 1308
Fragments arranged by size:
1646 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 976 1222 2000 2190 2982 976
Size: 246 778 190 792 2202
Fragments arranged by size:
2202 792 778 246 190
BsmI GAATG_Cn'
Cuts at: 756 756
Size: 4208
Bsp1286I G_dGCh'C
Cuts at: 976 1222 1833 1926 2000 2190 2252 2982 976
Size: 246 611 93 74 190 62 730 2202
Fragments arranged by size:
2202 730 611 246 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3428 3428
Size: 4208
BspHI T'CATG_A
Cuts at: 3384 3384
Size: 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 694 1035 1258 1472 1774 2155 694
Size: 341 223 214 302 381 2747
Fragments arranged by size:
2747 381 341 302 223 214
BsrBI CCG'CTC
Cuts at: 50 2500 2554 4038 4203 50
Size: 2450 54 1484 165 55
Fragments arranged by size:
2450 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 257 588 696 2120 257
Size: 331 108 1424 2345
Fragments arranged by size:
2345 1424 331 108
BsrFI r'CCGG_y
Cuts at: 2206 2387 2596 2206
Size: 181 209 3818
Fragments arranged by size:
3818 209 181
BssSI C'ACGA_G
Cuts at: 940 2479 2837 940
Size: 1539 358 2311
Fragments arranged by size:
2311 1539 358
BstAPI GCAn_nnn'nTGC
Cuts at: 170 1585 170
Size: 1415 2793
BstBI TT'CG_AA
Cuts at: 2569 2569
Size: 4208
BstEII G'GTnAC_C
Cuts at: 123 432 123
Size: 309 3899
BstYI r'GATC_y
Cuts at: 1 524 918 1072 1233 1700 1723 2058 2304
Size: 523 394 154 161 467 23 335 246
Cuts at: 2304 3316 3402 3414 3760 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 523 467 449 394 346 335 246
161 154 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2158 2339 2502 2158
Size: 181 163 3864
Fragments arranged by size:
3864 181 163
BtsI GCAGTG_nn'
Cuts at: 1451 1451
Size: 4208
DraI TTT'AAA
Cuts at: 1207 1207
Size: 4208
DrdI GACnn_nn'nnGTC
Cuts at: 1914 2772 1914
Size: 858 3350
EaeI y'GGCC_r
Cuts at: 775 1793 1967 2358 2385 3730 775
Size: 1018 174 391 27 1345 1253
Fragments arranged by size:
1345 1253 1018 391 174 27
EagI C'GGCC_G
Cuts at: 1793 1793
Size: 4208
EarI CTCTTCn'nnn_
Cuts at: 221 1075 2231 2441 221
Size: 854 1156 210 1988
Fragments arranged by size:
1988 1156 854 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2416 2726 2872 4018 2416
Size: 310 146 1146 2606
Fragments arranged by size:
2606 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1220 1220
Size: 4208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 448 555 983 1201 2033 2465 3212 3480
Size: 246 107 428 218 832 432 747 268
Cuts at: 3480 202
Size: 930
Fragments arranged by size:
930 832 747 432 428 268 246 218
107
EcoNI CCTnn'n_nnAGG
Cuts at: 649 649
Size: 4208
EcoO109I rG'GnC_Cy
Cuts at: 1347 1347
Size: 4208
EcoRI G'AATT_C
Cuts at: 763 1212 763
Size: 449 3759
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 4208
FspI TGC'GCA
Cuts at: 1989 1989
Size: 4208
HaeII r_GCGC'y
Cuts at: 1642 1890 2912 3891 1642
Size: 248 1022 979 1959
Fragments arranged by size:
1959 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 853 885 2050 2080 2082 2112 853
Size: 32 1165 30 2 30 2949
Fragments arranged by size:
2949 1165 32 30 30 2
HincII GTy'rAC
Cuts at: 252 660 1138 1247 252
Size: 408 478 109 3213
Fragments arranged by size:
3213 478 408 109
KasI G'GCGC_C
Cuts at: 1638 1886 1638
Size: 248 3960
KpnI G_GTAC'C
Cuts at: 1228 1228
Size: 4208
MluI A'CGCG_T
Cuts at: 3641 3641
Size: 4208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2879 3063 2879
Size: 184 4024
MscI TGG'CCA
Cuts at: 1969 1969
Size: 4208
MslI CAynn'nnrTG
Cuts at: 2197 2324 2197
Size: 127 4081
MspA1I CmG'CkG
Cuts at: 184 1319 1633 1993 3006 3251 184
Size: 1135 314 360 1013 245 1141
Fragments arranged by size:
1141 1135 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2389 2389
Size: 4208
NarI GG'CG_CC
Cuts at: 1639 1887 1639
Size: 248 3960
NgoMIV G'CCGG_C
Cuts at: 2387 2387
Size: 4208
NspI r_CATG'y
Cuts at: 1261 1761 2668 1261
Size: 500 907 2801
Fragments arranged by size:
2801 907 500
PciI A'CATG_T
Cuts at: 2664 2664
Size: 4208
PfoI T'CCnGG_A
Cuts at: 527 960 1060 1075 527
Size: 433 100 15 3660
Fragments arranged by size:
3660 433 100 15
PmeI GTTT'AAAC
Cuts at: 1207 1207
Size: 4208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 4176
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 625 657 625
Size: 32 4176
PstI C_TGCA'G
Cuts at: 1255 1940 1255
Size: 685 3523
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4208
PvuII CAG'CTG
Cuts at: 184 1633 1993 184
Size: 1449 360 2399
Fragments arranged by size:
2399 1449 360
RsrII CG'GwC_CG
Cuts at: 2403 2403
Size: 4208
SacI G_AGCT'C
Cuts at: 1222 1222
Size: 4208
SalI G'TCGA_C
Cuts at: 1245 1245
Size: 4208
SapI GCTCTTCn'nnn_
Cuts at: 2231 2441 2231
Size: 210 3998
SbfI CC_TGCA'GG
Cuts at: 1255 1255
Size: 4208
ScaI AGT'ACT
Cuts at: 3725 3725
Size: 4208
SfcI C'TryA_G
Cuts at: 33 508 1251 1936 2929 3120 33
Size: 475 743 685 993 191 1121
Fragments arranged by size:
1121 993 743 685 475 191
SfoI GGC'GCC
Cuts at: 1640 1888 1640
Size: 248 3960
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4208
SmaI CCC'GGG
Cuts at: 1230 1230
Size: 4208
SmlI C'TyrA_G
Cuts at: 148 375 1363 2278 2770 3032 148
Size: 227 988 915 492 262 1324
Fragments arranged by size:
1324 988 915 492 262 227
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4208
SphI G_CATG'C
Cuts at: 1261 1761 1261
Size: 500 3708
StyI C'CwwG_G
Cuts at: 816 884 1342 1696 816
Size: 68 458 354 3328
Fragments arranged by size:
3328 458 354 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1791 1924 2383 2592 1791
Size: 133 459 209 3407
Fragments arranged by size:
3407 459 209 133
TatI w'GTAC_w
Cuts at: 3723 3723
Size: 4208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1081 1113 3725 3757 1081
Size: 32 2612 32 1532
Fragments arranged by size:
2612 1532 32 32
Tth111I GACn'n_nGTC
Cuts at: 2005 2005
Size: 4208
XbaI T'CTAG_A
Cuts at: 1239 1239
Size: 4208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1703 1703
Size: 4208
XmaI C'CCGG_G
Cuts at: 1228 1228
Size: 4208
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmtI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI