Restriction map for
pF1KB6541
(Circular) (Six-base) MAPSORT of: pF1KB6541.seq Check: 3972 from: 1 to: 4196
pF1KB6541 4196 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 762 913 1234 762
Size: 151 321 3724
Fragments arranged by size:
3724 321 151
Acc65I G'GTAC_C
Cuts at: 497 1212 497
Size: 715 3481
AclI AA'CG_TT
Cuts at: 4036 4036
Size: 4196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2021 2453 3200 3468 2021
Size: 432 747 268 2749
Fragments arranged by size:
2749 747 432 268
AflIII A'CryG_T
Cuts at: 349 908 1548 2652 3629 349
Size: 559 640 1104 977 916
Fragments arranged by size:
1104 977 916 640 559
AgeI A'CCGG_T
Cuts at: 2584 2584
Size: 4196
AleI CACnn'nnGTG
Cuts at: 362 362
Size: 4196
AlwNI CAG_nnn'CTG
Cuts at: 1501 3068 1501
Size: 1567 2629
ApaI G_GGCC'C
Cuts at: 970 970
Size: 4196
ApaLI G'TGCA_C
Cuts at: 2966 2966
Size: 4196
ApoI r'AATT_y
Cuts at: 1065 1134 1200 3549 3805 1065
Size: 69 66 2349 256 1456
Fragments arranged by size:
2349 1456 256 69 66
AseI AT'TA_AT
Cuts at: 20 20
Size: 4196
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
AvaI C'yCGr_G
Cuts at: 161 1216 161
Size: 1055 3141
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 481 514 481
Size: 33 4163
BamHI G'GATC_C
Cuts at: 588 1221 588
Size: 633 3563
BanI G'GyrC_C
Cuts at: 497 660 1212 1626 1874 1909 497
Size: 163 552 414 248 35 2784
Fragments arranged by size:
2784 552 414 248 163 35
BanII G_rGCy'C
Cuts at: 213 970 1210 2240 213
Size: 757 240 1030 2169
Fragments arranged by size:
2169 1030 757 240
BbeI G_GCGC'C
Cuts at: 1630 1878 1630
Size: 248 3948
BbvCI CC'TCA_GC
Cuts at: 807 807
Size: 4196
BciVI GTATCCnnnnn_n'
Cuts at: 521 2094 2855 4196 521
Size: 1573 761 1341 521
Fragments arranged by size:
1573 1341 761 521
BclI T'GATC_A
Cuts at: 1716 1716
Size: 4196
BglII A'GATC_T
Cuts at: 1711 3748 1711
Size: 2037 2159
BlpI GC'TnA_GC
Cuts at: 1308 1308
Size: 4196
Bme1580I G_kGCm'C
Cuts at: 665 970 1105 1821 1914 2970 665
Size: 305 135 716 93 1056 1891
Fragments arranged by size:
1891 1056 716 305 135 93
BmgBI CAC'GTC
Cuts at: 343 343
Size: 4196
BmrI ACTGGGnnnn_n'
Cuts at: 401 572 1408 1489 1824 3968 401
Size: 171 836 81 335 2144 629
Fragments arranged by size:
2144 836 629 335 171 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 659 1182 2563 659
Size: 523 1381 2292
Fragments arranged by size:
2292 1381 523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 524 821 317
Size: 207 297 3692
Fragments arranged by size:
3692 297 207
Bpu10I CC'TnA_GC
Cuts at: 807 1038 807
Size: 231 3965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 1372 2251 2743 3041 345
Size: 1027 879 492 298 1500
Fragments arranged by size:
1500 1027 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 690 3900 690
Size: 3210 986
BsaAI yAC'GTr
Cuts at: 183 1549 183
Size: 1366 2830
BsaBI GATnn'nnATC
Cuts at: 6 1710 3747 6
Size: 1704 2037 455
Fragments arranged by size:
2037 1704 455
BsaHI Gr'CG_yC
Cuts at: 1627 1875 2577 4073 1627
Size: 248 702 1496 1750
Fragments arranged by size:
1750 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1385 1906 2584 2858 3005 3416 1385
Size: 521 678 274 147 411 2165
Fragments arranged by size:
2165 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 635 795 635
Size: 160 4036
BseYI C'CCAG_C
Cuts at: 1621 2379 2956 3452 1621
Size: 758 577 496 2365
Fragments arranged by size:
2365 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 347
Size: 4196
BsiEI CG_ry'CG
Cuts at: 104 1738 1784 2992 104
Size: 1634 46 1208 1308
Fragments arranged by size:
1634 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 213 1210 1988 2178 2970 213
Size: 997 778 190 792 1439
Fragments arranged by size:
1439 997 792 778 190
BsmI GAATG_Cn'
Cuts at: 714 734 930 714
Size: 20 196 3980
Fragments arranged by size:
3980 196 20
BsmBI CGTCTCn'nnnn_
Cuts at: 333 333
Size: 4196
Bsp1286I G_dGCh'C
Cuts at: 213 665 970 1105 1210 1821 1914 1988 2178
Size: 452 305 135 105 611 93 74 190
Cuts at: 2178 2240 2970 213
Size: 62 730 1439
Fragments arranged by size:
1439 730 611 452 305 190 135 105
93 74 62
BspEI T'CCGG_A
Cuts at: 3416 3416
Size: 4196
BspHI T'CATG_A
Cuts at: 3372 3372
Size: 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1246 1460 1762 2143 1246
Size: 214 302 381 3299
Fragments arranged by size:
3299 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2488 2542 4026 4191 50
Size: 2438 54 1484 165 55
Fragments arranged by size:
2438 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 698 2108 698
Size: 1410 2786
BsrFI r'CCGG_y
Cuts at: 2194 2375 2584 2194
Size: 181 209 3806
Fragments arranged by size:
3806 209 181
BssSI C'ACGA_G
Cuts at: 2467 2825 2467
Size: 358 3838
BstAPI GCAn_nnn'nTGC
Cuts at: 1573 1573
Size: 4196
BstBI TT'CG_AA
Cuts at: 2557 2557
Size: 4196
BstXI CCAn_nnnn'nTGG
Cuts at: 585 585
Size: 4196
BstYI r'GATC_y
Cuts at: 1 315 588 1221 1688 1711 2046 2292 3304
Size: 314 273 633 467 23 335 246 1012
Cuts at: 3304 3390 3402 3748 1
Size: 86 12 346 449
Fragments arranged by size:
1012 633 467 449 346 335 314 273
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 914 914
Size: 4196
Bsu36I CC'TnA_GG
Cuts at: 666 666
Size: 4196
BtgI C'CryG_G
Cuts at: 986 986
Size: 4196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2146 2327 2490 2146
Size: 181 163 3852
Fragments arranged by size:
3852 181 163
BtsI GCAGTG_nn'
Cuts at: 1439 1439
Size: 4196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1169 1204 1169
Size: 35 4161
DraI TTT'AAA
Cuts at: 1195 1195
Size: 4196
DrdI GACnn_nn'nnGTC
Cuts at: 1902 2760 1902
Size: 858 3338
EaeI y'GGCC_r
Cuts at: 769 1781 1955 2346 2373 3718 769
Size: 1012 174 391 27 1345 1247
Fragments arranged by size:
1345 1247 1012 391 174 27
EagI C'GGCC_G
Cuts at: 1781 1781
Size: 4196
EarI CTCTTCn'nnn_
Cuts at: 2219 2429 2219
Size: 210 3986
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2404 2714 2860 4006 2404
Size: 310 146 1146 2594
Fragments arranged by size:
2594 1146 310 146
EcoICRI GAG'CTC
Cuts at: 211 1208 211
Size: 997 3199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 524 821 2021 2453 3200 3468 317
Size: 207 297 1200 432 747 268 1045
Fragments arranged by size:
1200 1045 747 432 297 268 207
EcoNI CCTnn'n_nnAGG
Cuts at: 650 839 650
Size: 189 4007
EcoO109I rG'GnC_Cy
Cuts at: 454 515 832 967 1335 454
Size: 61 317 135 368 3315
Fragments arranged by size:
3315 368 317 135 61
EcoRI G'AATT_C
Cuts at: 1065 1200 1065
Size: 135 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 4196
FspI TGC'GCA
Cuts at: 1977 1977
Size: 4196
HaeII r_GCGC'y
Cuts at: 140 1630 1878 2900 3879 140
Size: 1490 248 1022 979 457
Fragments arranged by size:
1490 1022 979 457 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 519 551 2038 2068 2070 2100 519
Size: 32 1487 30 2 30 2615
Fragments arranged by size:
2615 1487 32 30 30 2
HincII GTy'rAC
Cuts at: 1235 1235
Size: 4196
KasI G'GCGC_C
Cuts at: 1626 1874 1626
Size: 248 3948
KpnI G_GTAC'C
Cuts at: 501 1216 501
Size: 715 3481
MluI A'CGCG_T
Cuts at: 3629 3629
Size: 4196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 173 616 2867 3051 173
Size: 443 2251 184 1318
Fragments arranged by size:
2251 1318 443 184
MscI TGG'CCA
Cuts at: 771 1957 771
Size: 1186 3010
MslI CAynn'nnrTG
Cuts at: 362 1022 1180 2185 2312 362
Size: 660 158 1005 127 2246
Fragments arranged by size:
2246 1005 660 158 127
MspA1I CmG'CkG
Cuts at: 1307 1621 1981 2994 3239 1307
Size: 314 360 1013 245 2264
Fragments arranged by size:
2264 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2377 2377
Size: 4196
NarI GG'CG_CC
Cuts at: 1627 1875 1627
Size: 248 3948
NcoI C'CATG_G
Cuts at: 986 986
Size: 4196
NgoMIV G'CCGG_C
Cuts at: 2375 2375
Size: 4196
NspI r_CATG'y
Cuts at: 353 447 912 1249 1749 2656 353
Size: 94 465 337 500 907 1893
Fragments arranged by size:
1893 907 500 465 337 94
PasI CC'CwG_GG
Cuts at: 970 970
Size: 4196
PciI A'CATG_T
Cuts at: 349 908 2652 349
Size: 559 1744 1893
Fragments arranged by size:
1893 1744 559
PflMI CCAn_nnn'nTGG
Cuts at: 439 757 844 439
Size: 318 87 3791
Fragments arranged by size:
3791 318 87
PfoI T'CCnGG_A
Cuts at: 837 936 837
Size: 99 4097
PmeI GTTT'AAAC
Cuts at: 1195 1195
Size: 4196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3335 3367 3335
Size: 32 4164
PpuMI rG'GwC_Cy
Cuts at: 454 832 454
Size: 378 3818
PspOMI G'GGCC_C
Cuts at: 966 966
Size: 4196
PstI C_TGCA'G
Cuts at: 399 1243 1928 399
Size: 844 685 2667
Fragments arranged by size:
2667 844 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4196
PvuII CAG'CTG
Cuts at: 1621 1981 1621
Size: 360 3836
RsrII CG'GwC_CG
Cuts at: 2391 2391
Size: 4196
SacI G_AGCT'C
Cuts at: 213 1210 213
Size: 997 3199
SalI G'TCGA_C
Cuts at: 1233 1233
Size: 4196
SapI GCTCTTCn'nnn_
Cuts at: 2219 2429 2219
Size: 210 3986
SbfI CC_TGCA'GG
Cuts at: 1243 1243
Size: 4196
ScaI AGT'ACT
Cuts at: 3713 3713
Size: 4196
SfcI C'TryA_G
Cuts at: 33 395 1239 1924 2917 3108 33
Size: 362 844 685 993 191 1121
Fragments arranged by size:
1121 993 844 685 362 191
SfoI GGC'GCC
Cuts at: 1628 1876 1628
Size: 248 3948
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
SmaI CCC'GGG
Cuts at: 163 1218 163
Size: 1055 3141
SmlI C'TyrA_G
Cuts at: 360 1351 2266 2758 3020 360
Size: 991 915 492 262 1536
Fragments arranged by size:
1536 991 915 492 262
SnaBI TAC'GTA
Cuts at: 183 183
Size: 4196
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4196
SphI G_CATG'C
Cuts at: 447 1249 1749 447
Size: 802 500 2894
Fragments arranged by size:
2894 802 500
StyI C'CwwG_G
Cuts at: 511 766 986 1108 1330 1684 511
Size: 255 220 122 222 354 3023
Fragments arranged by size:
3023 354 255 222 220 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 610 879 979 1779 1912 2371 2580 610
Size: 269 100 800 133 459 209 2226
Fragments arranged by size:
2226 800 459 269 209 133 100
TatI w'GTAC_w
Cuts at: 3711 3711
Size: 4196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3713 3745 3713
Size: 32 4164
Tth111I GACn'n_nGTC
Cuts at: 1993 1993
Size: 4196
XbaI T'CTAG_A
Cuts at: 1227 1227
Size: 4196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1691 1691
Size: 4196
XmaI C'CCGG_G
Cuts at: 161 1216 161
Size: 1055 3141
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BbsI BcgI BfrBI BglI BmtI BsaXI BsiWI
BsrGI BssHII BstEII ClaI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI
XmnI ZraI