Restriction map for
pF1KB6539
(Circular) (Six-base) MAPSORT of: pF1KB6539.seq Check: 3867 from: 1 to: 4217
pF1KB6539 4217 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 1255 1255
Size: 4217
Acc65I G'GTAC_C
Cuts at: 1233 1233
Size: 4217
AclI AA'CG_TT
Cuts at: 4057 4057
Size: 4217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2042 2474 3221 3489 2042
Size: 432 747 268 2770
Fragments arranged by size:
2770 747 432 268
AflIII A'CryG_T
Cuts at: 1569 2673 3650 1569
Size: 1104 977 2136
Fragments arranged by size:
2136 1104 977
AgeI A'CCGG_T
Cuts at: 2605 2605
Size: 4217
AlwNI CAG_nnn'CTG
Cuts at: 884 1522 3089 884
Size: 638 1567 2012
Fragments arranged by size:
2012 1567 638
ApaI G_GGCC'C
Cuts at: 582 723 909 582
Size: 141 186 3890
Fragments arranged by size:
3890 186 141
ApaLI G'TGCA_C
Cuts at: 2987 2987
Size: 4217
ApoI r'AATT_y
Cuts at: 1221 3570 3826 1221
Size: 2349 256 1612
Fragments arranged by size:
2349 1612 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4217
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
AvaI C'yCGr_G
Cuts at: 1237 1237
Size: 4217
BamHI G'GATC_C
Cuts at: 1242 1242
Size: 4217
BanI G'GyrC_C
Cuts at: 319 517 1233 1647 1895 1930 319
Size: 198 716 414 248 35 2606
Fragments arranged by size:
2606 716 414 248 198 35
BanII G_rGCy'C
Cuts at: 444 582 619 723 909 1171 1231 2261 444
Size: 138 37 104 186 262 60 1030 2400
Fragments arranged by size:
2400 1030 262 186 138 104 60 37
BbeI G_GCGC'C
Cuts at: 1651 1899 1651
Size: 248 3969
BciVI GTATCCnnnnn_n'
Cuts at: 264 2115 2876 4217 264
Size: 1851 761 1341 264
Fragments arranged by size:
1851 1341 761 264
BclI T'GATC_A
Cuts at: 1737 1737
Size: 4217
BglII A'GATC_T
Cuts at: 1732 3769 1732
Size: 2037 2180
BlpI GC'TnA_GC
Cuts at: 1329 1329
Size: 4217
Bme1580I G_kGCm'C
Cuts at: 582 723 909 1842 1935 2991 582
Size: 141 186 933 93 1056 1808
Fragments arranged by size:
1808 1056 933 186 141 93
BmrI ACTGGGnnnn_n'
Cuts at: 894 1429 1510 1845 3989 894
Size: 535 81 335 2144 1122
Fragments arranged by size:
2144 1122 535 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2584 2584
Size: 4217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 522
Size: 4217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 975 1393 2272 2764 3062 975
Size: 418 879 492 298 2130
Fragments arranged by size:
2130 879 492 418 298
BsaI GGTCTCn'nnnn_
Cuts at: 3921 3921
Size: 4217
BsaAI yAC'GTr
Cuts at: 1001 1570 1001
Size: 569 3648
BsaBI GATnn'nnATC
Cuts at: 6 391 1731 3768 6
Size: 385 1340 2037 455
Fragments arranged by size:
2037 1340 455 385
BsaHI Gr'CG_yC
Cuts at: 299 1648 1896 2598 4094 299
Size: 1349 248 702 1496 422
Fragments arranged by size:
1496 1349 702 422 248
BsaWI w'CCGG_w
Cuts at: 485 1406 1927 2605 2879 3026 3437 485
Size: 921 521 678 274 147 411 1265
Fragments arranged by size:
1265 921 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 546 847 850 871 546
Size: 301 3 21 3892
Fragments arranged by size:
3892 301 21 3
BseYI C'CCAG_C
Cuts at: 575 716 1642 2400 2977 3473 575
Size: 141 926 758 577 496 1319
Fragments arranged by size:
1319 926 758 577 496 141
BsiEI CG_ry'CG
Cuts at: 104 1759 1805 3013 104
Size: 1655 46 1208 1308
Fragments arranged by size:
1655 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 478 619 1231 2009 2199 2991 478
Size: 141 612 778 190 792 1704
Fragments arranged by size:
1704 792 778 612 190 141
Bsp1286I G_dGCh'C
Cuts at: 444 478 582 619 723 909 1171 1231 1842
Size: 34 104 37 104 186 262 60 611
Cuts at: 1842 1935 2009 2199 2261 2991 444
Size: 93 74 190 62 730 1670
Fragments arranged by size:
1670 730 611 262 190 186 104 104
93 74 62 60 37 34
BspEI T'CCGG_A
Cuts at: 3437 3437
Size: 4217
BspHI T'CATG_A
Cuts at: 411 3393 411
Size: 2982 1235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 343 1200 1267 1481 1783 2164 343
Size: 857 67 214 302 381 2396
Fragments arranged by size:
2396 857 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2509 2563 4047 4212 50
Size: 2459 54 1484 165 55
Fragments arranged by size:
2459 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 381 711 2129 381
Size: 330 1418 2469
Fragments arranged by size:
2469 1418 330
BsrFI r'CCGG_y
Cuts at: 2215 2396 2605 2215
Size: 181 209 3827
Fragments arranged by size:
3827 209 181
BssSI C'ACGA_G
Cuts at: 535 2488 2846 535
Size: 1953 358 1906
Fragments arranged by size:
1953 1906 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1594 1594
Size: 4217
BstBI TT'CG_AA
Cuts at: 2578 2578
Size: 4217
BstEII G'GTnAC_C
Cuts at: 1101 1101
Size: 4217
BstXI CCAn_nnnn'nTGG
Cuts at: 1074 1074
Size: 4217
BstYI r'GATC_y
Cuts at: 1 386 1242 1709 1732 2067 2313 3325 3411
Size: 385 856 467 23 335 246 1012 86
Cuts at: 3411 3423 3769 1
Size: 12 346 449
Fragments arranged by size:
1012 856 467 449 385 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 403 403
Size: 4217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 921 2167 2348 2511 921
Size: 1246 181 163 2627
Fragments arranged by size:
2627 1246 181 163
BtsI GCAGTG_nn'
Cuts at: 1138 1460 1138
Size: 322 3895
DraI TTT'AAA
Cuts at: 1216 1216
Size: 4217
DrdI GACnn_nn'nnGTC
Cuts at: 352 490 1923 2781 352
Size: 138 1433 858 1788
Fragments arranged by size:
1788 1433 858 138
EaeI y'GGCC_r
Cuts at: 843 1802 1976 2367 2394 3739 843
Size: 959 174 391 27 1345 1321
Fragments arranged by size:
1345 1321 959 391 174 27
EagI C'GGCC_G
Cuts at: 1802 1802
Size: 4217
EarI CTCTTCn'nnn_
Cuts at: 170 523 2240 2450 170
Size: 353 1717 210 1937
Fragments arranged by size:
1937 1717 353 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2425 2735 2881 4027 2425
Size: 310 146 1146 2615
Fragments arranged by size:
2615 1146 310 146
EcoICRI GAG'CTC
Cuts at: 617 1229 617
Size: 612 3605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 2042 2474 3221 3489 522
Size: 1520 432 747 268 1250
Fragments arranged by size:
1520 1250 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1111 1111
Size: 4217
EcoO109I rG'GnC_Cy
Cuts at: 418 579 720 906 1356 418
Size: 161 141 186 450 3279
Fragments arranged by size:
3279 450 186 161 141
EcoRI G'AATT_C
Cuts at: 1221 1221
Size: 4217
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 217 2236 217
Size: 2019 2198
FspI TGC'GCA
Cuts at: 373 1998 373
Size: 1625 2592
HaeII r_GCGC'y
Cuts at: 1651 1899 2921 3900 1651
Size: 248 1022 979 1968
Fragments arranged by size:
1968 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 371 403 742 774 973 1005 2059 2089 2091
Size: 32 339 32 199 32 1054 30 2
Cuts at: 2091 2121 371
Size: 30 2467
Fragments arranged by size:
2467 1054 339 199 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1155 1256 1155
Size: 101 4116
KasI G'GCGC_C
Cuts at: 1647 1895 1647
Size: 248 3969
KpnI G_GTAC'C
Cuts at: 1237 1237
Size: 4217
MluI A'CGCG_T
Cuts at: 3650 3650
Size: 4217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2888 3072 2888
Size: 184 4033
MscI TGG'CCA
Cuts at: 1978 1978
Size: 4217
MslI CAynn'nnrTG
Cuts at: 539 2206 2333 539
Size: 1667 127 2423
Fragments arranged by size:
2423 1667 127
MspA1I CmG'CkG
Cuts at: 716 944 1328 1642 2002 3015 3260 716
Size: 228 384 314 360 1013 245 1673
Fragments arranged by size:
1673 1013 384 360 314 245 228
NaeI GCC'GGC
Cuts at: 2398 2398
Size: 4217
NarI GG'CG_CC
Cuts at: 1648 1896 1648
Size: 248 3969
NgoMIV G'CCGG_C
Cuts at: 2396 2396
Size: 4217
NspI r_CATG'y
Cuts at: 544 1048 1270 1770 2677 544
Size: 504 222 500 907 2084
Fragments arranged by size:
2084 907 504 500 222
PciI A'CATG_T
Cuts at: 2673 2673
Size: 4217
PmeI GTTT'AAAC
Cuts at: 1216 1216
Size: 4217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3356 3388 3356
Size: 32 4185
PpuMI rG'GwC_Cy
Cuts at: 418 418
Size: 4217
PspOMI G'GGCC_C
Cuts at: 578 719 905 578
Size: 141 186 3890
Fragments arranged by size:
3890 186 141
PstI C_TGCA'G
Cuts at: 1264 1949 1264
Size: 685 3532
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4217
PvuII CAG'CTG
Cuts at: 716 944 1642 2002 716
Size: 228 698 360 2931
Fragments arranged by size:
2931 698 360 228
RsrII CG'GwC_CG
Cuts at: 2412 2412
Size: 4217
SacI G_AGCT'C
Cuts at: 619 1231 619
Size: 612 3605
SalI G'TCGA_C
Cuts at: 1254 1254
Size: 4217
SapI GCTCTTCn'nnn_
Cuts at: 523 2240 2450 523
Size: 1717 210 2290
Fragments arranged by size:
2290 1717 210
SbfI CC_TGCA'GG
Cuts at: 1264 1264
Size: 4217
ScaI AGT'ACT
Cuts at: 3734 3734
Size: 4217
SfcI C'TryA_G
Cuts at: 33 210 1260 1945 2938 3129 33
Size: 177 1050 685 993 191 1121
Fragments arranged by size:
1121 1050 993 685 191 177
SfoI GGC'GCC
Cuts at: 1649 1897 1649
Size: 248 3969
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
SmaI CCC'GGG
Cuts at: 1239 1239
Size: 4217
SmlI C'TyrA_G
Cuts at: 990 1372 2287 2779 3041 990
Size: 382 915 492 262 2166
Fragments arranged by size:
2166 915 492 382 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4217
SphI G_CATG'C
Cuts at: 544 1270 1770 544
Size: 726 500 2991
Fragments arranged by size:
2991 726 500
StyI C'CwwG_G
Cuts at: 1351 1705 1351
Size: 354 3863
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 770 1125 1800 1933 2392 2601 770
Size: 355 675 133 459 209 2386
Fragments arranged by size:
2386 675 459 355 209 133
TatI w'GTAC_w
Cuts at: 3732 3732
Size: 4217
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 832 864 3734 3766 832
Size: 32 2870 32 1283
Fragments arranged by size:
2870 1283 32 32
Tth111I GACn'n_nGTC
Cuts at: 2014 2014
Size: 4217
XbaI T'CTAG_A
Cuts at: 1248 1248
Size: 4217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1712 1712
Size: 4217
XmaI C'CCGG_G
Cuts at: 1237 1237
Size: 4217
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I BtgI ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI