Restriction map for
pF1KB6538
(Circular) (Six-base) MAPSORT of: pF1KB6538.seq Check: 1369 from: 1 to: 4286
pF1KB6538 4286 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 1324 1324
Size: 4286
Acc65I G'GTAC_C
Cuts at: 1302 1302
Size: 4286
AclI AA'CG_TT
Cuts at: 4126 4126
Size: 4286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 2111 2543 3290 3558 620
Size: 1491 432 747 268 1348
Fragments arranged by size:
1491 1348 747 432 268
AflIII A'CryG_T
Cuts at: 1638 2742 3719 1638
Size: 1104 977 2205
Fragments arranged by size:
2205 1104 977
AgeI A'CCGG_T
Cuts at: 2674 2674
Size: 4286
AhdI GACnn_n'nnGTC
Cuts at: 495 495
Size: 4286
AlwNI CAG_nnn'CTG
Cuts at: 315 726 930 1165 1591 3158 315
Size: 411 204 235 426 1567 1443
Fragments arranged by size:
1567 1443 426 411 235 204
ApaI G_GGCC'C
Cuts at: 1243 1243
Size: 4286
ApaLI G'TGCA_C
Cuts at: 3056 3056
Size: 4286
ApoI r'AATT_y
Cuts at: 769 1290 3639 3895 769
Size: 521 2349 256 1160
Fragments arranged by size:
2349 1160 521 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4286
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
AvaI C'yCGr_G
Cuts at: 1306 1306
Size: 4286
BamHI G'GATC_C
Cuts at: 1311 1311
Size: 4286
BanI G'GyrC_C
Cuts at: 1302 1716 1964 1999 1302
Size: 414 248 35 3589
Fragments arranged by size:
3589 414 248 35
BanII G_rGCy'C
Cuts at: 188 215 782 937 1063 1117 1154 1243 1300
Size: 27 567 155 126 54 37 89 57
Cuts at: 1300 2330 188
Size: 1030 2144
Fragments arranged by size:
2144 1030 567 155 126 89 57 54
37 27
BbeI G_GCGC'C
Cuts at: 1720 1968 1720
Size: 248 4038
BbsI GAAGACnn'nnnn_
Cuts at: 348 388 472 504 1268 348
Size: 40 84 32 764 3366
Fragments arranged by size:
3366 764 84 40 32
BbvCI CC'TCA_GC
Cuts at: 962 962
Size: 4286
BciVI GTATCCnnnnn_n'
Cuts at: 2184 2945 4286 2184
Size: 761 1341 2184
Fragments arranged by size:
2184 1341 761
BclI T'GATC_A
Cuts at: 1806 1806
Size: 4286
BglII A'GATC_T
Cuts at: 1801 3838 1801
Size: 2037 2249
BlpI GC'TnA_GC
Cuts at: 1148 1398 1148
Size: 250 4036
Bme1580I G_kGCm'C
Cuts at: 532 1243 1911 2004 3060 532
Size: 711 668 93 1056 1758
Fragments arranged by size:
1758 1056 711 668 93
BmrI ACTGGGnnnn_n'
Cuts at: 1148 1498 1579 1914 4058 1148
Size: 350 81 335 2144 1376
Fragments arranged by size:
2144 1376 350 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 4286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 818 952 397
Size: 421 134 3731
Fragments arranged by size:
3731 421 134
Bpu10I CC'TnA_GC
Cuts at: 784 962 784
Size: 178 4108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 1462 2341 2833 3131 748
Size: 714 879 492 298 1903
Fragments arranged by size:
1903 879 714 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3990 3990
Size: 4286
BsaAI yAC'GTr
Cuts at: 1639 1639
Size: 4286
BsaBI GATnn'nnATC
Cuts at: 6 1800 3837 6
Size: 1794 2037 455
Fragments arranged by size:
2037 1794 455
BsaHI Gr'CG_yC
Cuts at: 1717 1965 2667 4163 1717
Size: 248 702 1496 1840
Fragments arranged by size:
1840 1496 702 248
BsaWI w'CCGG_w
Cuts at: 221 1475 1996 2674 2948 3095 3506 221
Size: 1254 521 678 274 147 411 1001
Fragments arranged by size:
1254 1001 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 199 229 904 919 934 949 199
Size: 30 675 15 15 15 3536
Fragments arranged by size:
3536 675 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 225 670 673 724 1147 225
Size: 445 3 51 423 3364
Fragments arranged by size:
3364 445 423 51 3
BseYI C'CCAG_C
Cuts at: 519 549 609 1100 1243 1711 2469 3046 3542
Size: 30 60 491 143 468 758 577 496
Cuts at: 3542 519
Size: 1263
Fragments arranged by size:
1263 758 577 496 491 468 143 60
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 575
Size: 4286
BsiEI CG_ry'CG
Cuts at: 104 1828 1874 3082 104
Size: 1724 46 1208 1308
Fragments arranged by size:
1724 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 146 188 1300 2078 2268 3060 146
Size: 42 1112 778 190 792 1372
Fragments arranged by size:
1372 1112 792 778 190 42
BsmI GAATG_Cn'
Cuts at: 818 818
Size: 4286
Bsp1286I G_dGCh'C
Cuts at: 146 188 215 532 782 937 1063 1117 1154
Size: 42 27 317 250 155 126 54 37
Cuts at: 1154 1243 1300 1911 2004 2078 2268 2330 3060
Size: 89 57 611 93 74 190 62 730
Cuts at: 3060 146
Size: 1372
Fragments arranged by size:
1372 730 611 317 250 190 155 126
93 89 74 62 57 54 42 37
27
BspEI T'CCGG_A
Cuts at: 221 3506 221
Size: 3285 1001
BspHI T'CATG_A
Cuts at: 3462 3462
Size: 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 323 1336 1550 1852 2233 323
Size: 1013 214 302 381 2376
Fragments arranged by size:
2376 1013 381 302 214
BsrBI CCG'CTC
Cuts at: 50 242 273 362 1193 2578 2632 4116 4281
Size: 192 31 89 831 1385 54 1484 165
Cuts at: 4281 50
Size: 55
Fragments arranged by size:
1484 1385 831 192 165 89 55 54
31
BsrDI GCAATG_nn'
Cuts at: 444 1059 2198 444
Size: 615 1139 2532
Fragments arranged by size:
2532 1139 615
BsrFI r'CCGG_y
Cuts at: 2284 2465 2674 2284
Size: 181 209 3896
Fragments arranged by size:
3896 209 181
BssSI C'ACGA_G
Cuts at: 525 652 2557 2915 525
Size: 127 1905 358 1896
Fragments arranged by size:
1905 1896 358 127
BstAPI GCAn_nnn'nTGC
Cuts at: 1663 1663
Size: 4286
BstBI TT'CG_AA
Cuts at: 2647 2647
Size: 4286
BstXI CCAn_nnnn'nTGG
Cuts at: 684 684
Size: 4286
BstYI r'GATC_y
Cuts at: 1 329 1311 1778 1801 2136 2382 3394 3480
Size: 328 982 467 23 335 246 1012 86
Cuts at: 3480 3492 3838 1
Size: 12 346 449
Fragments arranged by size:
1012 982 467 449 346 335 328 246
86 23 12
BtgI C'CryG_G
Cuts at: 621 683 1034 621
Size: 62 351 3873
Fragments arranged by size:
3873 351 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 385 2236 2417 2580 385
Size: 1851 181 163 2091
Fragments arranged by size:
2091 1851 181 163
BtsI GCAGTG_nn'
Cuts at: 671 1529 671
Size: 858 3428
DraI TTT'AAA
Cuts at: 1285 1285
Size: 4286
DrdI GACnn_nn'nnGTC
Cuts at: 1992 2850 1992
Size: 858 3428
EaeI y'GGCC_r
Cuts at: 414 618 624 746 879 893 1871 2045 2436
Size: 204 6 122 133 14 978 174 391
Cuts at: 2436 2463 3808 414
Size: 27 1345 892
Fragments arranged by size:
1345 978 892 391 204 174 133 122
27 14 6
EagI C'GGCC_G
Cuts at: 1871 1871
Size: 4286
EarI CTCTTCn'nnn_
Cuts at: 280 2309 2519 280
Size: 2029 210 2047
Fragments arranged by size:
2047 2029 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1273 2494 2804 2950 4096 1273
Size: 1221 310 146 1146 1463
Fragments arranged by size:
1463 1221 1146 310 146
EcoICRI GAG'CTC
Cuts at: 186 1298 186
Size: 1112 3174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 620 818 952 2111 2543 3290 3558 397
Size: 223 198 134 1159 432 747 268 1125
Fragments arranged by size:
1159 1125 747 432 268 223 198 134
EcoNI CCTnn'n_nnAGG
Cuts at: 706 706
Size: 4286
EcoO109I rG'GnC_Cy
Cuts at: 312 927 1239 1240 1425 312
Size: 615 312 1 185 3173
Fragments arranged by size:
3173 615 312 185 1
EcoRI G'AATT_C
Cuts at: 769 1290 769
Size: 521 3765
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2305 2305
Size: 4286
FspI TGC'GCA
Cuts at: 643 2067 643
Size: 1424 2862
HaeII r_GCGC'y
Cuts at: 1720 1968 2990 3969 1720
Size: 248 1022 979 2037
Fragments arranged by size:
2037 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 199 231 481 508 513 540 898 904 919
Size: 32 250 27 5 27 358 6 15
Cuts at: 919 930 936 951 1003 1035 1104 1136 2128
Size: 11 6 15 52 32 69 32 992
Cuts at: 2128 2158 2160 2190 199
Size: 30 2 30 2295
Fragments arranged by size:
2295 992 358 250 69 52 32 32
32 30 30 27 27 15 15 11
6 6 5 2
HincII GTy'rAC
Cuts at: 1325 1325
Size: 4286
KasI G'GCGC_C
Cuts at: 1716 1964 1716
Size: 248 4038
KpnI G_GTAC'C
Cuts at: 1306 1306
Size: 4286
MluI A'CGCG_T
Cuts at: 3719 3719
Size: 4286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2957 3141 2957
Size: 184 4102
MscI TGG'CCA
Cuts at: 748 895 2047 748
Size: 147 1152 2987
Fragments arranged by size:
2987 1152 147
MslI CAynn'nnrTG
Cuts at: 827 2275 2402 827
Size: 1448 127 2711
Fragments arranged by size:
2711 1448 127
MspA1I CmG'CkG
Cuts at: 441 726 1397 1711 2071 3084 3329 441
Size: 285 671 314 360 1013 245 1398
Fragments arranged by size:
1398 1013 671 360 314 285 245
NaeI GCC'GGC
Cuts at: 2467 2467
Size: 4286
NarI GG'CG_CC
Cuts at: 1717 1965 1717
Size: 248 4038
NcoI C'CATG_G
Cuts at: 683 1034 683
Size: 351 3935
NgoMIV G'CCGG_C
Cuts at: 2465 2465
Size: 4286
NspI r_CATG'y
Cuts at: 540 1339 1839 2746 540
Size: 799 500 907 2080
Fragments arranged by size:
2080 907 799 500
PasI CC'CwG_GG
Cuts at: 1236 1236
Size: 4286
PciI A'CATG_T
Cuts at: 2742 2742
Size: 4286
PflMI CCAn_nnn'nTGG
Cuts at: 930 930
Size: 4286
PfoI T'CCnGG_A
Cuts at: 485 485
Size: 4286
PmeI GTTT'AAAC
Cuts at: 1285 1285
Size: 4286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 4254
PpuMI rG'GwC_Cy
Cuts at: 312 927 312
Size: 615 3671
PspOMI G'GGCC_C
Cuts at: 1239 1239
Size: 4286
PstI C_TGCA'G
Cuts at: 506 731 1333 2018 506
Size: 225 602 685 2774
Fragments arranged by size:
2774 685 602 225
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4286
PvuII CAG'CTG
Cuts at: 441 726 1711 2071 441
Size: 285 985 360 2656
Fragments arranged by size:
2656 985 360 285
RsrII CG'GwC_CG
Cuts at: 2481 2481
Size: 4286
SacI G_AGCT'C
Cuts at: 188 1300 188
Size: 1112 3174
SalI G'TCGA_C
Cuts at: 1323 1323
Size: 4286
SapI GCTCTTCn'nnn_
Cuts at: 280 2309 2519 280
Size: 2029 210 2047
Fragments arranged by size:
2047 2029 210
SbfI CC_TGCA'GG
Cuts at: 506 1333 506
Size: 827 3459
ScaI AGT'ACT
Cuts at: 3803 3803
Size: 4286
SfcI C'TryA_G
Cuts at: 33 502 727 1046 1329 2014 3007 3198 33
Size: 469 225 319 283 685 993 191 1121
Fragments arranged by size:
1121 993 685 469 319 283 225 191
SfoI GGC'GCC
Cuts at: 1718 1966 1718
Size: 248 4038
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
SmaI CCC'GGG
Cuts at: 1308 1308
Size: 4286
SmlI C'TyrA_G
Cuts at: 763 1441 2356 2848 3110 763
Size: 678 915 492 262 1939
Fragments arranged by size:
1939 915 678 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4286
SphI G_CATG'C
Cuts at: 1339 1839 1339
Size: 500 3786
StyI C'CwwG_G
Cuts at: 200 683 702 1034 1420 1774 200
Size: 483 19 332 386 354 2712
Fragments arranged by size:
2712 483 386 354 332 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1194 1869 2002 2461 2670 1194
Size: 675 133 459 209 2810
Fragments arranged by size:
2810 675 459 209 133
TatI w'GTAC_w
Cuts at: 1156 3801 1156
Size: 2645 1641
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 151 183 463 495 3803 3835 151
Size: 32 280 32 3308 32 602
Fragments arranged by size:
3308 602 280 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2083 2083
Size: 4286
XbaI T'CTAG_A
Cuts at: 1317 1317
Size: 4286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1251 1781 114
Size: 1137 530 2619
Fragments arranged by size:
2619 1137 530
XmaI C'CCGG_G
Cuts at: 1306 1306
Size: 4286
XmnI GAAnn'nnTTC
Cuts at: 343 343
Size: 4286
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI