Restriction map for
pF1KB6534
(Circular) (Six-base) MAPSORT of: pF1KB6534.seq Check: 114 from: 1 to: 4283
pF1KB6534 4283 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1321 1321
Size: 4283
Acc65I G'GTAC_C
Cuts at: 1299 1299
Size: 4283
AclI AA'CG_TT
Cuts at: 4123 4123
Size: 4283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 620 2108 2540 3287 3555 430
Size: 190 1488 432 747 268 1158
Fragments arranged by size:
1488 1158 747 432 268 190
AflIII A'CryG_T
Cuts at: 940 942 998 1635 2739 3716 940
Size: 2 56 637 1104 977 1507
Fragments arranged by size:
1507 1104 977 637 56 2
AgeI A'CCGG_T
Cuts at: 2671 2671
Size: 4283
AlwNI CAG_nnn'CTG
Cuts at: 1135 1588 3155 1135
Size: 453 1567 2263
Fragments arranged by size:
2263 1567 453
ApaLI G'TGCA_C
Cuts at: 3053 3053
Size: 4283
ApoI r'AATT_y
Cuts at: 111 379 481 673 703 1287 3636 3892 111
Size: 268 102 192 30 584 2349 256 502
Fragments arranged by size:
2349 584 502 268 256 192 102 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
AvaI C'yCGr_G
Cuts at: 1303 1303
Size: 4283
BamHI G'GATC_C
Cuts at: 1308 1308
Size: 4283
BanI G'GyrC_C
Cuts at: 1299 1713 1961 1996 1299
Size: 414 248 35 3586
Fragments arranged by size:
3586 414 248 35
BanII G_rGCy'C
Cuts at: 1297 2327 1297
Size: 1030 3253
BbeI G_GCGC'C
Cuts at: 1717 1965 1717
Size: 248 4035
BciVI GTATCCnnnnn_n'
Cuts at: 2181 2942 4283 2181
Size: 761 1341 2181
Fragments arranged by size:
2181 1341 761
BclI T'GATC_A
Cuts at: 285 1803 285
Size: 1518 2765
BfrBI ATG'CAT
Cuts at: 400 400
Size: 4283
BglII A'GATC_T
Cuts at: 825 1798 3835 825
Size: 973 2037 1273
Fragments arranged by size:
2037 1273 973
BlpI GC'TnA_GC
Cuts at: 1395 1395
Size: 4283
Bme1580I G_kGCm'C
Cuts at: 1908 2001 3057 1908
Size: 93 1056 3134
Fragments arranged by size:
3134 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1495 1576 1911 4055 1495
Size: 81 335 2144 1723
Fragments arranged by size:
2144 1723 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 4283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 799 853 1106 509
Size: 290 54 253 3686
Fragments arranged by size:
3686 290 253 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 976 1459 2338 2830 3128 976
Size: 483 879 492 298 2131
Fragments arranged by size:
2131 879 492 483 298
BsaI GGTCTCn'nnnn_
Cuts at: 871 1078 3987 871
Size: 207 2909 1167
Fragments arranged by size:
2909 1167 207
BsaAI yAC'GTr
Cuts at: 943 1636 943
Size: 693 3590
BsaBI GATnn'nnATC
Cuts at: 6 1164 1269 1797 3834 6
Size: 1158 105 528 2037 455
Fragments arranged by size:
2037 1158 528 455 105
BsaHI Gr'CG_yC
Cuts at: 1714 1962 2664 4160 1714
Size: 248 702 1496 1837
Fragments arranged by size:
1837 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1472 1993 2671 2945 3092 3503 1472
Size: 521 678 274 147 411 2252
Fragments arranged by size:
2252 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1056 1086 1056
Size: 30 4253
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1135 1135
Size: 4283
BseYI C'CCAG_C
Cuts at: 1708 2466 3043 3539 1708
Size: 758 577 496 2452
Fragments arranged by size:
2452 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 239
Size: 4283
BsiEI CG_ry'CG
Cuts at: 104 1825 1871 3079 104
Size: 1721 46 1208 1308
Fragments arranged by size:
1721 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1297 2075 2265 3057 1297
Size: 778 190 792 2523
Fragments arranged by size:
2523 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1297 1908 2001 2075 2265 2327 3057 1297
Size: 611 93 74 190 62 730 2523
Fragments arranged by size:
2523 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3503 3503
Size: 4283
BspHI T'CATG_A
Cuts at: 3459 3459
Size: 4283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1333 1547 1849 2230 1333
Size: 214 302 381 3386
Fragments arranged by size:
3386 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2575 2629 4113 4278 50
Size: 2525 54 1484 165 55
Fragments arranged by size:
2525 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2195 2195
Size: 4283
BsrFI r'CCGG_y
Cuts at: 2281 2462 2671 2281
Size: 181 209 3893
Fragments arranged by size:
3893 209 181
BsrGI T'GTAC_A
Cuts at: 786 786
Size: 4283
BssSI C'ACGA_G
Cuts at: 1075 2554 2912 1075
Size: 1479 358 2446
Fragments arranged by size:
2446 1479 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1135 1660 1135
Size: 525 3758
BstBI TT'CG_AA
Cuts at: 2644 2644
Size: 4283
BstXI CCAn_nnnn'nTGG
Cuts at: 801 1017 801
Size: 216 4067
BstYI r'GATC_y
Cuts at: 1 825 1308 1775 1798 2133 2379 3391 3477
Size: 824 483 467 23 335 246 1012 86
Cuts at: 3477 3489 3835 1
Size: 12 346 449
Fragments arranged by size:
1012 824 483 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 1010 1068 1010
Size: 58 4225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2233 2414 2577 2233
Size: 181 163 3939
Fragments arranged by size:
3939 181 163
BtsI GCAGTG_nn'
Cuts at: 251 667 890 1526 251
Size: 416 223 636 3008
Fragments arranged by size:
3008 636 416 223
ClaI AT'CG_AT
Cuts at: 860 860
Size: 4283
DraI TTT'AAA
Cuts at: 1282 1282
Size: 4283
DraIII CAC_nnn'GTG
Cuts at: 121 121
Size: 4283
DrdI GACnn_nn'nnGTC
Cuts at: 1989 2847 1989
Size: 858 3425
EaeI y'GGCC_r
Cuts at: 709 1868 2042 2433 2460 3805 709
Size: 1159 174 391 27 1345 1187
Fragments arranged by size:
1345 1187 1159 391 174 27
EagI C'GGCC_G
Cuts at: 1868 1868
Size: 4283
EarI CTCTTCn'nnn_
Cuts at: 433 875 971 1184 1258 2306 2516 433
Size: 442 96 213 74 1048 210 2200
Fragments arranged by size:
2200 1048 442 213 210 96 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2491 2801 2947 4093 2491
Size: 310 146 1146 2681
Fragments arranged by size:
2681 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1295 1295
Size: 4283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 509 620 799 853 1106 2108 2540 3287
Size: 79 111 179 54 253 1002 432 747
Cuts at: 3287 3555 430
Size: 268 1158
Fragments arranged by size:
1158 1002 747 432 268 253 179 111
79 54
EcoNI CCTnn'n_nnAGG
Cuts at: 1096 1096
Size: 4283
EcoO109I rG'GnC_Cy
Cuts at: 798 1422 798
Size: 624 3659
EcoRI G'AATT_C
Cuts at: 111 379 1287 111
Size: 268 908 3107
Fragments arranged by size:
3107 908 268
EcoRV GAT'ATC
Cuts at: 1026 1026
Size: 4283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 4283
FspI TGC'GCA
Cuts at: 2064 2064
Size: 4283
HaeII r_GCGC'y
Cuts at: 1717 1965 2987 3966 1717
Size: 248 1022 979 2034
Fragments arranged by size:
2034 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1056 1088 2125 2155 2157 2187 1056
Size: 32 1037 30 2 30 3152
Fragments arranged by size:
3152 1037 32 30 30 2
HincII GTy'rAC
Cuts at: 1322 1322
Size: 4283
HindIII A'AGCT_T
Cuts at: 367 874 367
Size: 507 3776
KasI G'GCGC_C
Cuts at: 1713 1961 1713
Size: 248 4035
KpnI G_GTAC'C
Cuts at: 1303 1303
Size: 4283
MluI A'CGCG_T
Cuts at: 3716 3716
Size: 4283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 180 2954 3138 180
Size: 2774 184 1325
Fragments arranged by size:
2774 1325 184
MscI TGG'CCA
Cuts at: 711 2044 711
Size: 1333 2950
MslI CAynn'nnrTG
Cuts at: 739 838 1015 2272 2399 739
Size: 99 177 1257 127 2623
Fragments arranged by size:
2623 1257 177 127 99
MspA1I CmG'CkG
Cuts at: 1070 1141 1150 1394 1708 2068 3081 3326 1070
Size: 71 9 244 314 360 1013 245 2027
Fragments arranged by size:
2027 1013 360 314 245 244 71 9
NaeI GCC'GGC
Cuts at: 2464 2464
Size: 4283
NarI GG'CG_CC
Cuts at: 1714 1962 1714
Size: 248 4035
NcoI C'CATG_G
Cuts at: 1010 1010
Size: 4283
NdeI CA'TA_TG
Cuts at: 402 402
Size: 4283
NgoMIV G'CCGG_C
Cuts at: 2462 2462
Size: 4283
NsiI A_TGCA'T
Cuts at: 402 402
Size: 4283
NspI r_CATG'y
Cuts at: 1336 1836 2743 1336
Size: 500 907 2876
Fragments arranged by size:
2876 907 500
PciI A'CATG_T
Cuts at: 2739 2739
Size: 4283
PfoI T'CCnGG_A
Cuts at: 800 800
Size: 4283
PmeI GTTT'AAAC
Cuts at: 1282 1282
Size: 4283
PmlI CAC'GTG
Cuts at: 943 943
Size: 4283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 4251
PpuMI rG'GwC_Cy
Cuts at: 798 798
Size: 4283
PshAI GACnn'nnGTC
Cuts at: 943 943
Size: 4283
PstI C_TGCA'G
Cuts at: 1140 1330 2015 1140
Size: 190 685 3408
Fragments arranged by size:
3408 685 190
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4283
PvuII CAG'CTG
Cuts at: 1141 1708 2068 1141
Size: 567 360 3356
Fragments arranged by size:
3356 567 360
RsrII CG'GwC_CG
Cuts at: 2478 2478
Size: 4283
SacI G_AGCT'C
Cuts at: 1297 1297
Size: 4283
SacII CC_GC'GG
Cuts at: 1071 1071
Size: 4283
SalI G'TCGA_C
Cuts at: 1320 1320
Size: 4283
SapI GCTCTTCn'nnn_
Cuts at: 433 2306 2516 433
Size: 1873 210 2200
Fragments arranged by size:
2200 1873 210
SbfI CC_TGCA'GG
Cuts at: 1330 1330
Size: 4283
ScaI AGT'ACT
Cuts at: 3800 3800
Size: 4283
SfcI C'TryA_G
Cuts at: 33 1136 1151 1326 2011 3004 3195 33
Size: 1103 15 175 685 993 191 1121
Fragments arranged by size:
1121 1103 993 685 191 175 15
SfoI GGC'GCC
Cuts at: 1715 1963 1715
Size: 248 4035
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
SmaI CCC'GGG
Cuts at: 1305 1305
Size: 4283
SmlI C'TyrA_G
Cuts at: 991 1438 2353 2845 3107 991
Size: 447 915 492 262 2167
Fragments arranged by size:
2167 915 492 447 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4283
SphI G_CATG'C
Cuts at: 1336 1836 1336
Size: 500 3783
SspI AAT'ATT
Cuts at: 463 609 463
Size: 146 4137
StuI AGG'CCT
Cuts at: 1102 1102
Size: 4283
StyI C'CwwG_G
Cuts at: 794 1010 1417 1771 794
Size: 216 407 354 3306
Fragments arranged by size:
3306 407 354 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 546 1866 1999 2458 2667 546
Size: 1320 133 459 209 2162
Fragments arranged by size:
2162 1320 459 209 133
TatI w'GTAC_w
Cuts at: 786 3798 786
Size: 3012 1271
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3800 3832 3800
Size: 32 4251
Tth111I GACn'n_nGTC
Cuts at: 2080 2080
Size: 4283
XbaI T'CTAG_A
Cuts at: 1314 1314
Size: 4283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1017 1778 1017
Size: 761 3522
XmaI C'CCGG_G
Cuts at: 1303 1303
Size: 4283
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BssHII BstEII
BstZ17I Bsu36I CspCI FseI FspAI HpaI MfeI NheI
NotI NruI PacI PasI PflMI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI XmnI ZraI