Restriction map for
pF1KB6533
(Circular) (Six-base) MAPSORT of: pF1KB6533.seq Check: 189 from: 1 to: 4214
pF1KB6533 4214 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 526 1252 526
Size: 726 3488
Acc65I G'GTAC_C
Cuts at: 1230 1230
Size: 4214
AclI AA'CG_TT
Cuts at: 4054 4054
Size: 4214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 987 1162 2039 2471 3218 3486 234
Size: 753 175 877 432 747 268 962
Fragments arranged by size:
962 877 753 747 432 268 175
AflIII A'CryG_T
Cuts at: 1566 2670 3647 1566
Size: 1104 977 2133
Fragments arranged by size:
2133 1104 977
AgeI A'CCGG_T
Cuts at: 2602 2602
Size: 4214
AleI CACnn'nnGTG
Cuts at: 259 1117 259
Size: 858 3356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 277 703 277
Size: 426 3788
AlwNI CAG_nnn'CTG
Cuts at: 596 812 1023 1044 1069 1519 3086 596
Size: 216 211 21 25 450 1567 1724
Fragments arranged by size:
1724 1567 450 216 211 25 21
ApaLI G'TGCA_C
Cuts at: 2984 2984
Size: 4214
ApoI r'AATT_y
Cuts at: 1218 3567 3823 1218
Size: 2349 256 1609
Fragments arranged by size:
2349 1609 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4214
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
AvaI C'yCGr_G
Cuts at: 1234 1234
Size: 4214
BamHI G'GATC_C
Cuts at: 1239 1239
Size: 4214
BanI G'GyrC_C
Cuts at: 124 266 280 340 391 406 1102 1230 1644
Size: 142 14 60 51 15 696 128 414
Cuts at: 1644 1892 1927 124
Size: 248 35 2411
Fragments arranged by size:
2411 696 414 248 142 128 60 51
35 15 14
BanII G_rGCy'C
Cuts at: 320 542 1078 1186 1228 2258 320
Size: 222 536 108 42 1030 2276
Fragments arranged by size:
2276 1030 536 222 108 42
BbeI G_GCGC'C
Cuts at: 270 284 344 395 1648 1896 270
Size: 14 60 51 1253 248 2588
Fragments arranged by size:
2588 1253 248 60 51 14
BbsI GAAGACnn'nnnn_
Cuts at: 453 453
Size: 4214
BciVI GTATCCnnnnn_n'
Cuts at: 472 2112 2873 4214 472
Size: 1640 761 1341 472
Fragments arranged by size:
1640 1341 761 472
BclI T'GATC_A
Cuts at: 716 1734 716
Size: 1018 3196
BglI GCCn_nnn'nGGC
Cuts at: 421 887 421
Size: 466 3748
BglII A'GATC_T
Cuts at: 1729 3766 1729
Size: 2037 2177
BlpI GC'TnA_GC
Cuts at: 541 1168 1326 541
Size: 627 158 3429
Fragments arranged by size:
3429 627 158
Bme1580I G_kGCm'C
Cuts at: 127 308 1839 1932 2988 127
Size: 181 1531 93 1056 1353
Fragments arranged by size:
1531 1353 1056 181 93
BmrI ACTGGGnnnn_n'
Cuts at: 1426 1507 1842 3986 1426
Size: 81 335 2144 1654
Fragments arranged by size:
2144 1654 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 4214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 313 878 158
Size: 155 565 3494
Fragments arranged by size:
3494 565 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1390 2269 2761 3059 1390
Size: 879 492 298 2545
Fragments arranged by size:
2545 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3918 3918
Size: 4214
BsaAI yAC'GTr
Cuts at: 1567 1567
Size: 4214
BsaBI GATnn'nnATC
Cuts at: 6 1728 3765 6
Size: 1722 2037 455
Fragments arranged by size:
2037 1722 455
BsaHI Gr'CG_yC
Cuts at: 267 281 341 392 1645 1893 2595 4091 267
Size: 14 60 51 1253 248 702 1496 390
Fragments arranged by size:
1496 1253 702 390 248 60 51 14
BsaWI w'CCGG_w
Cuts at: 1403 1924 2602 2876 3023 3434 1403
Size: 521 678 274 147 411 2183
Fragments arranged by size:
2183 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 181 211 793 823 851 881 1182 1212 181
Size: 30 582 30 28 30 301 30 3183
Fragments arranged by size:
3183 582 301 30 30 30 30 28
BseYI C'CCAG_C
Cuts at: 156 1069 1201 1639 2397 2974 3470 156
Size: 913 132 438 758 577 496 900
Fragments arranged by size:
913 900 758 577 496 438 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 534 609 534
Size: 75 4139
BsiEI CG_ry'CG
Cuts at: 104 327 425 1098 1756 1802 3010 104
Size: 223 98 673 658 46 1208 1308
Fragments arranged by size:
1308 1208 673 658 223 98 46
BsiHKAI G_wGCw'C
Cuts at: 299 320 1094 1228 2006 2196 2988 299
Size: 21 774 134 778 190 792 1525
Fragments arranged by size:
1525 792 778 774 190 134 21
BsmI GAATG_Cn'
Cuts at: 858 858
Size: 4214
BsmBI CGTCTCn'nnnn_
Cuts at: 729 1140 729
Size: 411 3803
Bsp1286I G_dGCh'C
Cuts at: 127 299 308 320 542 1078 1094 1186 1228
Size: 172 9 12 222 536 16 92 42
Cuts at: 1228 1839 1932 2006 2196 2258 2988 127
Size: 611 93 74 190 62 730 1353
Fragments arranged by size:
1353 730 611 536 222 190 172 93
92 74 62 42 16 12 9
BspEI T'CCGG_A
Cuts at: 3434 3434
Size: 4214
BspHI T'CATG_A
Cuts at: 3390 3390
Size: 4214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1264 1478 1780 2161 1264
Size: 214 302 381 3317
Fragments arranged by size:
3317 381 302 214
BsrBI CCG'CTC
Cuts at: 50 169 197 2506 2560 4044 4209 50
Size: 119 28 2309 54 1484 165 55
Fragments arranged by size:
2309 1484 165 119 55 54 28
BsrDI GCAATG_nn'
Cuts at: 181 2126 181
Size: 1945 2269
BsrFI r'CCGG_y
Cuts at: 217 229 337 424 2212 2393 2602 217
Size: 12 108 87 1788 181 209 1829
Fragments arranged by size:
1829 1788 209 181 108 87 12
BssHII G'CGCG_C
Cuts at: 428 445 447 428
Size: 17 2 4195
Fragments arranged by size:
4195 17 2
BssSI C'ACGA_G
Cuts at: 2485 2843 2485
Size: 358 3856
BstAPI GCAn_nnn'nTGC
Cuts at: 180 303 596 1591 180
Size: 123 293 995 2803
Fragments arranged by size:
2803 995 293 123
BstBI TT'CG_AA
Cuts at: 2575 2575
Size: 4214
BstXI CCAn_nnnn'nTGG
Cuts at: 114 291 114
Size: 177 4037
BstYI r'GATC_y
Cuts at: 1 1239 1706 1729 2064 2310 3322 3408 3420
Size: 1238 467 23 335 246 1012 86 12
Cuts at: 3420 3766 1
Size: 346 449
Fragments arranged by size:
1238 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 284 321 353 533 1082 107
Size: 177 37 32 180 549 3239
Fragments arranged by size:
3239 549 180 177 37 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 210 2164 2345 2508 210
Size: 1954 181 163 1916
Fragments arranged by size:
1954 1916 181 163
BtsI GCAGTG_nn'
Cuts at: 666 876 1457 666
Size: 210 581 3423
Fragments arranged by size:
3423 581 210
DraI TTT'AAA
Cuts at: 1213 1213
Size: 4214
DraIII CAC_nnn'GTG
Cuts at: 303 354 303
Size: 51 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1920 2778 1920
Size: 858 3356
EaeI y'GGCC_r
Cuts at: 219 324 335 422 1799 1973 2364 2391 3736
Size: 105 11 87 1377 174 391 27 1345
Cuts at: 3736 219
Size: 697
Fragments arranged by size:
1377 1345 697 391 174 105 87 27
11
EagI C'GGCC_G
Cuts at: 324 422 1799 324
Size: 98 1377 2739
Fragments arranged by size:
2739 1377 98
EarI CTCTTCn'nnn_
Cuts at: 2237 2447 2237
Size: 210 4004
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2422 2732 2878 4024 2422
Size: 310 146 1146 2612
Fragments arranged by size:
2612 1146 310 146
EcoICRI GAG'CTC
Cuts at: 318 1226 318
Size: 908 3306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 234 313 878 987 1162 2039 2471 3218
Size: 76 79 565 109 175 877 432 747
Cuts at: 3218 3486 158
Size: 268 886
Fragments arranged by size:
886 877 747 565 432 268 175 109
79 76
EcoO109I rG'GnC_Cy
Cuts at: 1353 1353
Size: 4214
EcoRI G'AATT_C
Cuts at: 1218 1218
Size: 4214
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 4214
FspI TGC'GCA
Cuts at: 1995 1995
Size: 4214
HaeII r_GCGC'y
Cuts at: 154 270 284 344 380 395 1648 1896 2918
Size: 116 14 60 36 15 1253 248 1022
Cuts at: 2918 3897 154
Size: 979 471
Fragments arranged by size:
1253 1022 979 471 248 116 60 36
15 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 181 213 793 825 921 953 2056 2086 2088
Size: 32 580 32 96 32 1103 30 2
Cuts at: 2088 2118 181
Size: 30 2277
Fragments arranged by size:
2277 1103 580 96 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 240 527 1253 240
Size: 287 726 3201
Fragments arranged by size:
3201 726 287
KasI G'GCGC_C
Cuts at: 266 280 340 391 1644 1892 266
Size: 14 60 51 1253 248 2588
Fragments arranged by size:
2588 1253 248 60 51 14
KpnI G_GTAC'C
Cuts at: 1234 1234
Size: 4214
MfeI C'AATT_G
Cuts at: 732 732
Size: 4214
MluI A'CGCG_T
Cuts at: 3647 3647
Size: 4214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2885 3069 2885
Size: 184 4030
MscI TGG'CCA
Cuts at: 1975 1975
Size: 4214
MslI CAynn'nnrTG
Cuts at: 112 118 259 778 1087 1117 2203 2330 112
Size: 6 141 519 309 30 1086 127 1996
Fragments arranged by size:
1996 1086 519 309 141 127 30 6
MspA1I CmG'CkG
Cuts at: 323 1020 1205 1325 1639 1999 3012 3257 323
Size: 697 185 120 314 360 1013 245 1280
Fragments arranged by size:
1280 1013 697 360 314 245 185 120
NaeI GCC'GGC
Cuts at: 219 231 339 426 2395 219
Size: 12 108 87 1969 2038
Fragments arranged by size:
2038 1969 108 87 12
NarI GG'CG_CC
Cuts at: 267 281 341 392 1645 1893 267
Size: 14 60 51 1253 248 2588
Fragments arranged by size:
2588 1253 248 60 51 14
NcoI C'CATG_G
Cuts at: 107 284 533 1082 107
Size: 177 249 549 3239
Fragments arranged by size:
3239 549 249 177
NgoMIV G'CCGG_C
Cuts at: 217 229 337 424 2393 217
Size: 12 108 87 1969 2038
Fragments arranged by size:
2038 1969 108 87 12
NotI GC'GGCC_GC
Cuts at: 324 324
Size: 4214
NspI r_CATG'y
Cuts at: 690 1267 1767 2674 690
Size: 577 500 907 2230
Fragments arranged by size:
2230 907 577 500
PciI A'CATG_T
Cuts at: 2670 2670
Size: 4214
PflMI CCAn_nnn'nTGG
Cuts at: 354 1044 354
Size: 690 3524
PmeI GTTT'AAAC
Cuts at: 1213 1213
Size: 4214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3353 3385 3353
Size: 32 4182
PstI C_TGCA'G
Cuts at: 601 1028 1261 1946 601
Size: 427 233 685 2869
Fragments arranged by size:
2869 685 427 233
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4214
PvuII CAG'CTG
Cuts at: 1020 1205 1639 1999 1020
Size: 185 434 360 3235
Fragments arranged by size:
3235 434 360 185
RsrII CG'GwC_CG
Cuts at: 2409 2409
Size: 4214
SacI G_AGCT'C
Cuts at: 320 1228 320
Size: 908 3306
SacII CC_GC'GG
Cuts at: 324 324
Size: 4214
SalI G'TCGA_C
Cuts at: 525 1251 525
Size: 726 3488
SapI GCTCTTCn'nnn_
Cuts at: 2237 2447 2237
Size: 210 4004
SbfI CC_TGCA'GG
Cuts at: 1261 1261
Size: 4214
ScaI AGT'ACT
Cuts at: 558 3731 558
Size: 3173 1041
SfcI C'TryA_G
Cuts at: 33 597 1024 1257 1942 2935 3126 33
Size: 564 427 233 685 993 191 1121
Fragments arranged by size:
1121 993 685 564 427 233 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 421 421
Size: 4214
SfoI GGC'GCC
Cuts at: 268 282 342 393 1646 1894 268
Size: 14 60 51 1253 248 2588
Fragments arranged by size:
2588 1253 248 60 51 14
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4214
SmaI CCC'GGG
Cuts at: 1236 1236
Size: 4214
SmlI C'TyrA_G
Cuts at: 1369 2284 2776 3038 1369
Size: 915 492 262 2545
Fragments arranged by size:
2545 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4214
SphI G_CATG'C
Cuts at: 1267 1767 1267
Size: 500 3714
StuI AGG'CCT
Cuts at: 143 958 143
Size: 815 3399
StyI C'CwwG_G
Cuts at: 107 275 284 533 698 1082 1348 1702 107
Size: 168 9 249 165 384 266 354 2619
Fragments arranged by size:
2619 384 354 266 249 168 165 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 575 1112 1797 1930 2389 2598 575
Size: 537 685 133 459 209 2191
Fragments arranged by size:
2191 685 537 459 209 133
TatI w'GTAC_w
Cuts at: 556 764 3729 556
Size: 208 2965 1041
Fragments arranged by size:
2965 1041 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 571 603 851 883 3731 3763 571
Size: 32 248 32 2848 32 1022
Fragments arranged by size:
2848 1022 248 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2011 2011
Size: 4214
XbaI T'CTAG_A
Cuts at: 1245 1245
Size: 4214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 282 1709 282
Size: 1427 2787
XmaI C'CCGG_G
Cuts at: 1234 1234
Size: 4214
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BseRI BsiWI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI NdeI NheI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI