Restriction map for
pF1KB6532
(Circular) (Six-base) MAPSORT of: pF1KB6532.seq Check: 9569 from: 1 to: 4280
pF1KB6532 4280 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 360 719 1318 360
Size: 359 599 3322
Fragments arranged by size:
3322 599 359
Acc65I G'GTAC_C
Cuts at: 1296 1296
Size: 4280
AclI AA'CG_TT
Cuts at: 874 4120 874
Size: 3246 1034
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 268 323 403 833 1172 2105 2537 3284
Size: 150 55 80 430 339 933 432 747
Cuts at: 3284 3552 118
Size: 268 846
Fragments arranged by size:
933 846 747 432 430 339 268 150
80 55
AflII C'TTAA_G
Cuts at: 184 184
Size: 4280
AflIII A'CryG_T
Cuts at: 470 1632 2736 3713 470
Size: 1162 1104 977 1037
Fragments arranged by size:
1162 1104 1037 977
AgeI A'CCGG_T
Cuts at: 2668 2668
Size: 4280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 169 169
Size: 4280
AlwNI CAG_nnn'CTG
Cuts at: 1585 3152 1585
Size: 1567 2713
ApaLI G'TGCA_C
Cuts at: 3050 3050
Size: 4280
ApoI r'AATT_y
Cuts at: 310 1284 3633 3889 310
Size: 974 2349 256 701
Fragments arranged by size:
2349 974 701 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4280
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
AvaI C'yCGr_G
Cuts at: 1300 1300
Size: 4280
BamHI G'GATC_C
Cuts at: 453 963 1305 453
Size: 510 342 3428
Fragments arranged by size:
3428 510 342
BanI G'GyrC_C
Cuts at: 1296 1710 1958 1993 1296
Size: 414 248 35 3583
Fragments arranged by size:
3583 414 248 35
BanII G_rGCy'C
Cuts at: 1294 2324 1294
Size: 1030 3250
BbeI G_GCGC'C
Cuts at: 1714 1962 1714
Size: 248 4032
BbsI GAAGACnn'nnnn_
Cuts at: 1208 1208
Size: 4280
BciVI GTATCCnnnnn_n'
Cuts at: 2178 2939 4280 2178
Size: 761 1341 2178
Fragments arranged by size:
2178 1341 761
BclI T'GATC_A
Cuts at: 749 932 1179 1800 749
Size: 183 247 621 3229
Fragments arranged by size:
3229 621 247 183
BfrBI ATG'CAT
Cuts at: 791 791
Size: 4280
BglII A'GATC_T
Cuts at: 1131 1795 3832 1131
Size: 664 2037 1579
Fragments arranged by size:
2037 1579 664
BlpI GC'TnA_GC
Cuts at: 1392 1392
Size: 4280
Bme1580I G_kGCm'C
Cuts at: 1905 1998 3054 1905
Size: 93 1056 3131
Fragments arranged by size:
3131 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1492 1573 1908 4052 1492
Size: 81 335 2144 1720
Fragments arranged by size:
2144 1720 335 81
BmtI G_CTAG'C
Cuts at: 674 674
Size: 4280
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 4280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 1267
Size: 4280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1456 2335 2827 3125 1456
Size: 879 492 298 2611
Fragments arranged by size:
2611 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3984 3984
Size: 4280
BsaAI yAC'GTr
Cuts at: 1633 1633
Size: 4280
BsaBI GATnn'nnATC
Cuts at: 6 1794 3831 6
Size: 1788 2037 455
Fragments arranged by size:
2037 1788 455
BsaHI Gr'CG_yC
Cuts at: 1711 1959 2661 4157 1711
Size: 248 702 1496 1834
Fragments arranged by size:
1834 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1469 1990 2668 2942 3089 3500 1469
Size: 521 678 274 147 411 2249
Fragments arranged by size:
2249 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1705 2463 3040 3536 1705
Size: 758 577 496 2449
Fragments arranged by size:
2449 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 180 1822 1868 3076 104
Size: 76 1642 46 1208 1308
Fragments arranged by size:
1642 1308 1208 76 46
BsiHKAI G_wGCw'C
Cuts at: 744 1294 2072 2262 3054 744
Size: 550 778 190 792 1970
Fragments arranged by size:
1970 792 778 550 190
Bsp1286I G_dGCh'C
Cuts at: 744 1294 1905 1998 2072 2262 2324 3054 744
Size: 550 611 93 74 190 62 730 1970
Fragments arranged by size:
1970 730 611 550 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3500 3500
Size: 4280
BspHI T'CATG_A
Cuts at: 428 477 1182 1208 3456 428
Size: 49 705 26 2248 1252
Fragments arranged by size:
2248 1252 705 49 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1048 1147 1330 1544 1846 2227 1048
Size: 99 183 214 302 381 3101
Fragments arranged by size:
3101 381 302 214 183 99
BsrBI CCG'CTC
Cuts at: 50 2572 2626 4110 4275 50
Size: 2522 54 1484 165 55
Fragments arranged by size:
2522 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2192 2192
Size: 4280
BsrFI r'CCGG_y
Cuts at: 2278 2459 2668 2278
Size: 181 209 3890
Fragments arranged by size:
3890 209 181
BssSI C'ACGA_G
Cuts at: 659 2551 2909 659
Size: 1892 358 2030
Fragments arranged by size:
2030 1892 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1174 1657 1174
Size: 483 3797
BstBI TT'CG_AA
Cuts at: 2641 2641
Size: 4280
BstYI r'GATC_y
Cuts at: 1 453 530 963 1131 1305 1772 1795 2130
Size: 452 77 433 168 174 467 23 335
Cuts at: 2130 2376 3388 3474 3486 3832 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 452 449 433 346 335 246
174 168 86 77 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2230 2411 2574 2230
Size: 181 163 3936
Fragments arranged by size:
3936 181 163
BtsI GCAGTG_nn'
Cuts at: 1523 1523
Size: 4280
DraI TTT'AAA
Cuts at: 1279 1279
Size: 4280
DrdI GACnn_nn'nnGTC
Cuts at: 1986 2844 1986
Size: 858 3422
EaeI y'GGCC_r
Cuts at: 177 1865 2039 2430 2457 3802 177
Size: 1688 174 391 27 1345 655
Fragments arranged by size:
1688 1345 655 391 174 27
EagI C'GGCC_G
Cuts at: 177 1865 177
Size: 1688 2592
EarI CTCTTCn'nnn_
Cuts at: 2303 2513 2303
Size: 210 4070
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2488 2798 2944 4090 127
Size: 2361 310 146 1146 317
Fragments arranged by size:
2361 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 1292 1292
Size: 4280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 268 323 403 833 1172 1267 2105 2537
Size: 150 55 80 430 339 95 838 432
Cuts at: 2537 3284 3552 118
Size: 747 268 846
Fragments arranged by size:
846 838 747 432 430 339 268 150
95 80 55
EcoNI CCTnn'n_nnAGG
Cuts at: 608 608
Size: 4280
EcoO109I rG'GnC_Cy
Cuts at: 252 483 1060 1419 252
Size: 231 577 359 3113
Fragments arranged by size:
3113 577 359 231
EcoRI G'AATT_C
Cuts at: 1284 1284
Size: 4280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 419 2299 419
Size: 1880 2400
FspI TGC'GCA
Cuts at: 2061 2061
Size: 4280
HaeII r_GCGC'y
Cuts at: 133 1714 1962 2984 3963 133
Size: 1581 248 1022 979 450
Fragments arranged by size:
1581 1022 979 450 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2122 2152 2154 2184 2122
Size: 30 2 30 4218
Fragments arranged by size:
4218 30 30 2
HincII GTy'rAC
Cuts at: 299 361 1319 299
Size: 62 958 3260
Fragments arranged by size:
3260 958 62
HindIII A'AGCT_T
Cuts at: 1105 1105
Size: 4280
HpaI GTT'AAC
Cuts at: 299 299
Size: 4280
KasI G'GCGC_C
Cuts at: 1710 1958 1710
Size: 248 4032
KpnI G_GTAC'C
Cuts at: 1300 1300
Size: 4280
MfeI C'AATT_G
Cuts at: 1097 1097
Size: 4280
MluI A'CGCG_T
Cuts at: 3713 3713
Size: 4280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2951 3135 2951
Size: 184 4096
MscI TGG'CCA
Cuts at: 2041 2041
Size: 4280
MslI CAynn'nnrTG
Cuts at: 799 2269 2396 799
Size: 1470 127 2683
Fragments arranged by size:
2683 1470 127
MspA1I CmG'CkG
Cuts at: 678 853 1391 1705 2065 3078 3323 678
Size: 175 538 314 360 1013 245 1635
Fragments arranged by size:
1635 1013 538 360 314 245 175
NaeI GCC'GGC
Cuts at: 2461 2461
Size: 4280
NarI GG'CG_CC
Cuts at: 1711 1959 1711
Size: 248 4032
NcoI C'CATG_G
Cuts at: 107 107
Size: 4280
NgoMIV G'CCGG_C
Cuts at: 2459 2459
Size: 4280
NheI G'CTAG_C
Cuts at: 670 670
Size: 4280
NsiI A_TGCA'T
Cuts at: 793 793
Size: 4280
NspI r_CATG'y
Cuts at: 474 1333 1833 2740 474
Size: 859 500 907 2014
Fragments arranged by size:
2014 907 859 500
PasI CC'CwG_GG
Cuts at: 487 487
Size: 4280
PciI A'CATG_T
Cuts at: 470 2736 470
Size: 2266 2014
PfoI T'CCnGG_A
Cuts at: 533 981 533
Size: 448 3832
PmeI GTTT'AAAC
Cuts at: 1279 1279
Size: 4280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3419 3451 3419
Size: 32 4248
PpuMI rG'GwC_Cy
Cuts at: 483 1060 483
Size: 577 3703
PshAI GACnn'nnGTC
Cuts at: 179 179
Size: 4280
PsiI TTA'TAA
Cuts at: 467 467
Size: 4280
PstI C_TGCA'G
Cuts at: 1327 2012 1327
Size: 685 3595
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4280
PvuII CAG'CTG
Cuts at: 678 853 1705 2065 678
Size: 175 852 360 2893
Fragments arranged by size:
2893 852 360 175
RsrII CG'GwC_CG
Cuts at: 2475 2475
Size: 4280
SacI G_AGCT'C
Cuts at: 1294 1294
Size: 4280
SalI G'TCGA_C
Cuts at: 359 1317 359
Size: 958 3322
SapI GCTCTTCn'nnn_
Cuts at: 2303 2513 2303
Size: 210 4070
SbfI CC_TGCA'GG
Cuts at: 1327 1327
Size: 4280
ScaI AGT'ACT
Cuts at: 3797 3797
Size: 4280
SexAI A'CCwGG_T
Cuts at: 633 633
Size: 4280
SfcI C'TryA_G
Cuts at: 33 256 1323 2008 3001 3192 33
Size: 223 1067 685 993 191 1121
Fragments arranged by size:
1121 1067 993 685 223 191
SfoI GGC'GCC
Cuts at: 1712 1960 1712
Size: 248 4032
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4280
SmaI CCC'GGG
Cuts at: 1302 1302
Size: 4280
SmlI C'TyrA_G
Cuts at: 184 1435 2350 2842 3104 184
Size: 1251 915 492 262 1360
Fragments arranged by size:
1360 1251 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4280
SphI G_CATG'C
Cuts at: 1333 1833 1333
Size: 500 3780
StyI C'CwwG_G
Cuts at: 107 1414 1768 107
Size: 1307 354 2619
Fragments arranged by size:
2619 1307 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 268 1863 1996 2455 2664 268
Size: 1595 133 459 209 1884
Fragments arranged by size:
1884 1595 459 209 133
TatI w'GTAC_w
Cuts at: 725 1147 1194 3795 725
Size: 422 47 2601 1210
Fragments arranged by size:
2601 1210 422 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3797 3829 3797
Size: 32 4248
Tth111I GACn'n_nGTC
Cuts at: 2077 2077
Size: 4280
XbaI T'CTAG_A
Cuts at: 1311 1311
Size: 4280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1775 1775
Size: 4280
XmaI C'CCGG_G
Cuts at: 1300 1300
Size: 4280
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI NspI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI Bpu10I BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI NdeI NotI NruI PacI PflMI PmlI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI