Restriction map for
pF1KB6521
(Circular) (Six-base) MAPSORT of: pF1KB6521.seq Check: 29 from: 1 to: 4202
pF1KB6521 4202 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 1240 1240
Size: 4202
Acc65I G'GTAC_C
Cuts at: 1218 1218
Size: 4202
AclI AA'CG_TT
Cuts at: 4042 4042
Size: 4202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 2459 3206 3474 2027
Size: 432 747 268 2755
Fragments arranged by size:
2755 747 432 268
AfeI AGC'GCT
Cuts at: 849 849
Size: 4202
AflII C'TTAA_G
Cuts at: 819 819
Size: 4202
AflIII A'CryG_T
Cuts at: 1554 2658 3635 1554
Size: 1104 977 2121
Fragments arranged by size:
2121 1104 977
AgeI A'CCGG_T
Cuts at: 2590 2590
Size: 4202
AlwNI CAG_nnn'CTG
Cuts at: 1507 3074 1507
Size: 1567 2635
ApaLI G'TGCA_C
Cuts at: 2972 2972
Size: 4202
ApoI r'AATT_y
Cuts at: 480 694 722 1206 3555 3811 480
Size: 214 28 484 2349 256 871
Fragments arranged by size:
2349 871 484 256 214 28
AseI AT'TA_AT
Cuts at: 20 20
Size: 4202
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4202
AvaI C'yCGr_G
Cuts at: 1222 1222
Size: 4202
BamHI G'GATC_C
Cuts at: 1227 1227
Size: 4202
BanI G'GyrC_C
Cuts at: 1218 1632 1880 1915 1218
Size: 414 248 35 3505
Fragments arranged by size:
3505 414 248 35
BanII G_rGCy'C
Cuts at: 1216 2246 1216
Size: 1030 3172
BbeI G_GCGC'C
Cuts at: 1636 1884 1636
Size: 248 3954
BbvCI CC'TCA_GC
Cuts at: 576 576
Size: 4202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4168
BciVI GTATCCnnnnn_n'
Cuts at: 2100 2861 4202 2100
Size: 761 1341 2100
Fragments arranged by size:
2100 1341 761
BclI T'GATC_A
Cuts at: 1722 1722
Size: 4202
BfrBI ATG'CAT
Cuts at: 436 436
Size: 4202
BglII A'GATC_T
Cuts at: 315 1717 3754 315
Size: 1402 2037 763
Fragments arranged by size:
2037 1402 763
BlpI GC'TnA_GC
Cuts at: 1314 1314
Size: 4202
Bme1580I G_kGCm'C
Cuts at: 1827 1920 2976 1827
Size: 93 1056 3053
Fragments arranged by size:
3053 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1414 1495 1830 3974 1414
Size: 81 335 2144 1642
Fragments arranged by size:
2144 1642 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2569 2569
Size: 4202
Bpu10I CC'TnA_GC
Cuts at: 576 584 576
Size: 8 4194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1378 2257 2749 3047 445
Size: 933 879 492 298 1600
Fragments arranged by size:
1600 933 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3906 3906
Size: 4202
BsaAI yAC'GTr
Cuts at: 1555 1555
Size: 4202
BsaBI GATnn'nnATC
Cuts at: 6 1716 3753 6
Size: 1710 2037 455
Fragments arranged by size:
2037 1710 455
BsaHI Gr'CG_yC
Cuts at: 1633 1881 2583 4079 1633
Size: 248 702 1496 1756
Fragments arranged by size:
1756 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1391 1912 2590 2864 3011 3422 1391
Size: 521 678 274 147 411 2171
Fragments arranged by size:
2171 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 601 631 601
Size: 30 4172
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 182 182
Size: 4202
BseYI C'CCAG_C
Cuts at: 904 1627 2385 2962 3458 904
Size: 723 758 577 496 1648
Fragments arranged by size:
1648 758 723 577 496
BsiEI CG_ry'CG
Cuts at: 104 1744 1790 2998 104
Size: 1640 46 1208 1308
Fragments arranged by size:
1640 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1216 1994 2184 2976 1216
Size: 778 190 792 2442
Fragments arranged by size:
2442 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1216 1827 1920 1994 2184 2246 2976 1216
Size: 611 93 74 190 62 730 2442
Fragments arranged by size:
2442 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3422 3422
Size: 4202
BspHI T'CATG_A
Cuts at: 3378 3378
Size: 4202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1252 1466 1768 2149 1252
Size: 214 302 381 3305
Fragments arranged by size:
3305 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2494 2548 4032 4197 50
Size: 2444 54 1484 165 55
Fragments arranged by size:
2444 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 746 899 2114 746
Size: 153 1215 2834
Fragments arranged by size:
2834 1215 153
BsrFI r'CCGG_y
Cuts at: 2200 2381 2590 2200
Size: 181 209 3812
Fragments arranged by size:
3812 209 181
BssSI C'ACGA_G
Cuts at: 2473 2831 2473
Size: 358 3844
BstAPI GCAn_nnn'nTGC
Cuts at: 1129 1579 1129
Size: 450 3752
BstBI TT'CG_AA
Cuts at: 2563 2563
Size: 4202
BstXI CCAn_nnnn'nTGG
Cuts at: 372 372
Size: 4202
BstYI r'GATC_y
Cuts at: 1 315 1227 1694 1717 2052 2298 3310 3396
Size: 314 912 467 23 335 246 1012 86
Cuts at: 3396 3408 3754 1
Size: 12 346 449
Fragments arranged by size:
1012 912 467 449 346 335 314 246
86 23 12
BtgI C'CryG_G
Cuts at: 605 605
Size: 4202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2152 2333 2496 2152
Size: 181 163 3858
Fragments arranged by size:
3858 181 163
BtsI GCAGTG_nn'
Cuts at: 293 1445 293
Size: 1152 3050
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 233 268 233
Size: 35 4167
DraI TTT'AAA
Cuts at: 1201 1201
Size: 4202
DrdI GACnn_nn'nnGTC
Cuts at: 1908 2766 1908
Size: 858 3344
EaeI y'GGCC_r
Cuts at: 1787 1961 2352 2379 3724 1787
Size: 174 391 27 1345 2265
Fragments arranged by size:
2265 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1787 1787
Size: 4202
EarI CTCTTCn'nnn_
Cuts at: 2225 2435 2225
Size: 210 3992
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 521 2410 2720 2866 4012 521
Size: 1889 310 146 1146 711
Fragments arranged by size:
1889 1146 711 310 146
EcoICRI GAG'CTC
Cuts at: 1214 1214
Size: 4202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 2459 3206 3474 2027
Size: 432 747 268 2755
Fragments arranged by size:
2755 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1341 1341
Size: 4202
EcoRI G'AATT_C
Cuts at: 1206 1206
Size: 4202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 136 2221 136
Size: 2085 2117
FspI TGC'GCA
Cuts at: 1983 1983
Size: 4202
HaeII r_GCGC'y
Cuts at: 851 1636 1884 2906 3885 851
Size: 785 248 1022 979 1168
Fragments arranged by size:
1168 1022 979 785 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 147 179 601 633 2044 2074 2076 2106 147
Size: 32 422 32 1411 30 2 30 2243
Fragments arranged by size:
2243 1411 422 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1110 1241 1110
Size: 131 4071
KasI G'GCGC_C
Cuts at: 1632 1880 1632
Size: 248 3954
KpnI G_GTAC'C
Cuts at: 1222 1222
Size: 4202
MluI A'CGCG_T
Cuts at: 3635 3635
Size: 4202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2873 3057 2873
Size: 184 4018
MscI TGG'CCA
Cuts at: 1963 1963
Size: 4202
MslI CAynn'nnrTG
Cuts at: 2191 2318 2191
Size: 127 4075
MspA1I CmG'CkG
Cuts at: 153 580 919 1313 1627 1987 3000 3245 153
Size: 427 339 394 314 360 1013 245 1110
Fragments arranged by size:
1110 1013 427 394 360 339 314 245
NaeI GCC'GGC
Cuts at: 2383 2383
Size: 4202
NarI GG'CG_CC
Cuts at: 1633 1881 1633
Size: 248 3954
NcoI C'CATG_G
Cuts at: 605 605
Size: 4202
NgoMIV G'CCGG_C
Cuts at: 2381 2381
Size: 4202
NsiI A_TGCA'T
Cuts at: 438 438
Size: 4202
NspI r_CATG'y
Cuts at: 1255 1755 2662 1255
Size: 500 907 2795
Fragments arranged by size:
2795 907 500
PasI CC'CwG_GG
Cuts at: 224 224
Size: 4202
PciI A'CATG_T
Cuts at: 2658 2658
Size: 4202
PflMI CCAn_nnn'nTGG
Cuts at: 1044 1044
Size: 4202
PfoI T'CCnGG_A
Cuts at: 269 269
Size: 4202
PmeI GTTT'AAAC
Cuts at: 1201 1201
Size: 4202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 3373 3341
Size: 32 4170
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 437 469 437
Size: 32 4170
PstI C_TGCA'G
Cuts at: 1249 1934 1249
Size: 685 3517
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4202
PvuII CAG'CTG
Cuts at: 580 919 1627 1987 580
Size: 339 708 360 2795
Fragments arranged by size:
2795 708 360 339
RsrII CG'GwC_CG
Cuts at: 2397 2397
Size: 4202
SacI G_AGCT'C
Cuts at: 1216 1216
Size: 4202
SalI G'TCGA_C
Cuts at: 1239 1239
Size: 4202
SapI GCTCTTCn'nnn_
Cuts at: 2225 2435 2225
Size: 210 3992
SbfI CC_TGCA'GG
Cuts at: 1249 1249
Size: 4202
ScaI AGT'ACT
Cuts at: 141 3719 141
Size: 3578 624
SfcI C'TryA_G
Cuts at: 33 1245 1930 2923 3114 33
Size: 1212 685 993 191 1121
Fragments arranged by size:
1212 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1634 1882 1634
Size: 248 3954
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4202
SmaI CCC'GGG
Cuts at: 1224 1224
Size: 4202
SmlI C'TyrA_G
Cuts at: 424 819 1357 2272 2764 3026 424
Size: 395 538 915 492 262 1600
Fragments arranged by size:
1600 915 538 492 395 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4202
SphI G_CATG'C
Cuts at: 1255 1755 1255
Size: 500 3702
StyI C'CwwG_G
Cuts at: 158 605 1336 1690 158
Size: 447 731 354 2670
Fragments arranged by size:
2670 731 447 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 216 1785 1918 2377 2586 216
Size: 1569 133 459 209 1832
Fragments arranged by size:
1832 1569 459 209 133
TatI w'GTAC_w
Cuts at: 139 3717 139
Size: 3578 624
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3719 3751 3719
Size: 32 4170
Tth111I GACn'n_nGTC
Cuts at: 1999 1999
Size: 4202
XbaI T'CTAG_A
Cuts at: 682 1233 682
Size: 551 3651
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1697 1697
Size: 4202
XmaI C'CCGG_G
Cuts at: 1222 1222
Size: 4202
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BglI BmgBI BmtI BpmI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PmlI PpuMI
PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI