Restriction map for
pF1KB6515
(Circular) (Six-base) MAPSORT of: pF1KB6515.seq Check: 7394 from: 1 to: 4193
pF1KB6515 4193 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1231 1231
Size: 4193
Acc65I G'GTAC_C
Cuts at: 1209 1209
Size: 4193
AclI AA'CG_TT
Cuts at: 1187 4033 1187
Size: 2846 1347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 733 2018 2450 3197 3465 526
Size: 207 1285 432 747 268 1254
Fragments arranged by size:
1285 1254 747 432 268 207
AflII C'TTAA_G
Cuts at: 1104 1104
Size: 4193
AflIII A'CryG_T
Cuts at: 550 1545 2649 3626 550
Size: 995 1104 977 1117
Fragments arranged by size:
1117 1104 995 977
AgeI A'CCGG_T
Cuts at: 2581 2581
Size: 4193
AleI CACnn'nnGTG
Cuts at: 560 560
Size: 4193
AlwNI CAG_nnn'CTG
Cuts at: 275 1498 3065 275
Size: 1223 1567 1403
Fragments arranged by size:
1567 1403 1223
ApaLI G'TGCA_C
Cuts at: 2963 2963
Size: 4193
ApoI r'AATT_y
Cuts at: 1197 3546 3802 1197
Size: 2349 256 1588
Fragments arranged by size:
2349 1588 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
AvaI C'yCGr_G
Cuts at: 944 1213 944
Size: 269 3924
BamHI G'GATC_C
Cuts at: 1218 1218
Size: 4193
BanI G'GyrC_C
Cuts at: 365 1209 1623 1871 1906 365
Size: 844 414 248 35 2652
Fragments arranged by size:
2652 844 414 248 35
BanII G_rGCy'C
Cuts at: 1030 1207 2237 1030
Size: 177 1030 2986
Fragments arranged by size:
2986 1030 177
BbeI G_GCGC'C
Cuts at: 1627 1875 1627
Size: 248 3945
BbsI GAAGACnn'nnnn_
Cuts at: 135 514 829 135
Size: 379 315 3499
Fragments arranged by size:
3499 379 315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4159
BciVI GTATCCnnnnn_n'
Cuts at: 2091 2852 4193 2091
Size: 761 1341 2091
Fragments arranged by size:
2091 1341 761
BclI T'GATC_A
Cuts at: 596 1713 596
Size: 1117 3076
BglII A'GATC_T
Cuts at: 798 1708 3745 798
Size: 910 2037 1246
Fragments arranged by size:
2037 1246 910
BlpI GC'TnA_GC
Cuts at: 1305 1305
Size: 4193
Bme1580I G_kGCm'C
Cuts at: 1818 1911 2967 1818
Size: 93 1056 3044
Fragments arranged by size:
3044 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 725 1405 1486 1821 3965 725
Size: 680 81 335 2144 953
Fragments arranged by size:
2144 953 680 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 626 1070 478
Size: 148 444 3601
Fragments arranged by size:
3601 444 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1369 2248 2740 3038 1369
Size: 879 492 298 2524
Fragments arranged by size:
2524 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3897 3897
Size: 4193
BsaAI yAC'GTr
Cuts at: 1546 1546
Size: 4193
BsaBI GATnn'nnATC
Cuts at: 6 1707 3744 6
Size: 1701 2037 455
Fragments arranged by size:
2037 1701 455
BsaHI Gr'CG_yC
Cuts at: 1624 1872 2574 4070 1624
Size: 248 702 1496 1747
Fragments arranged by size:
1747 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1382 1903 2581 2855 3002 3413 1382
Size: 521 678 274 147 411 2162
Fragments arranged by size:
2162 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 195 225 1017 1047 195
Size: 30 792 30 3341
Fragments arranged by size:
3341 792 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 200 203 653 656 659 683 1038 200
Size: 3 450 3 3 24 355 3355
Fragments arranged by size:
3355 450 355 24 3 3 3
BseYI C'CCAG_C
Cuts at: 1618 2376 2953 3449 1618
Size: 758 577 496 2362
Fragments arranged by size:
2362 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1735 1781 2989 104
Size: 1631 46 1208 1308
Fragments arranged by size:
1631 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1207 1985 2175 2967 1207
Size: 778 190 792 2433
Fragments arranged by size:
2433 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 897 897
Size: 4193
Bsp1286I G_dGCh'C
Cuts at: 1030 1207 1818 1911 1985 2175 2237 2967 1030
Size: 177 611 93 74 190 62 730 2256
Fragments arranged by size:
2256 730 611 190 177 93 74 62
BspEI T'CCGG_A
Cuts at: 3413 3413
Size: 4193
BspHI T'CATG_A
Cuts at: 3369 3369
Size: 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1243 1457 1759 2140 1243
Size: 214 302 381 3296
Fragments arranged by size:
3296 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2485 2539 4023 4188 50
Size: 2435 54 1484 165 55
Fragments arranged by size:
2435 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2105 2105
Size: 4193
BsrFI r'CCGG_y
Cuts at: 2191 2372 2581 2191
Size: 181 209 3803
Fragments arranged by size:
3803 209 181
BssSI C'ACGA_G
Cuts at: 2464 2822 2464
Size: 358 3835
BstAPI GCAn_nnn'nTGC
Cuts at: 1570 1570
Size: 4193
BstBI TT'CG_AA
Cuts at: 2554 2554
Size: 4193
BstYI r'GATC_y
Cuts at: 1 798 987 1218 1685 1708 2043 2289 3301
Size: 797 189 231 467 23 335 246 1012
Cuts at: 3301 3387 3399 3745 1
Size: 86 12 346 449
Fragments arranged by size:
1012 797 467 449 346 335 246 231
189 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 845 2143 2324 2487 845
Size: 1298 181 163 2551
Fragments arranged by size:
2551 1298 181 163
BtsI GCAGTG_nn'
Cuts at: 634 1436 634
Size: 802 3391
DraI TTT'AAA
Cuts at: 1192 1192
Size: 4193
DrdI GACnn_nn'nnGTC
Cuts at: 1899 2757 1899
Size: 858 3335
EaeI y'GGCC_r
Cuts at: 1778 1952 2343 2370 3715 1778
Size: 174 391 27 1345 2256
Fragments arranged by size:
2256 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1778 1778
Size: 4193
EarI CTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2401 2711 2857 4003 2401
Size: 310 146 1146 2591
Fragments arranged by size:
2591 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1205 1205
Size: 4193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 526 626 733 1070 2018 2450 3197 3465
Size: 48 100 107 337 948 432 747 268
Cuts at: 3465 478
Size: 1206
Fragments arranged by size:
1206 948 747 432 337 268 107 100
48
EcoO109I rG'GnC_Cy
Cuts at: 1332 1332
Size: 4193
EcoRI G'AATT_C
Cuts at: 1197 1197
Size: 4193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 640 2212 640
Size: 1572 2621
FspI TGC'GCA
Cuts at: 1974 1974
Size: 4193
HaeII r_GCGC'y
Cuts at: 1627 1875 2897 3876 1627
Size: 248 1022 979 1944
Fragments arranged by size:
1944 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2035 2065 2067 2097 2035
Size: 30 2 30 4131
Fragments arranged by size:
4131 30 30 2
HincII GTy'rAC
Cuts at: 1232 1232
Size: 4193
HindIII A'AGCT_T
Cuts at: 850 850
Size: 4193
KasI G'GCGC_C
Cuts at: 1623 1871 1623
Size: 248 3945
KpnI G_GTAC'C
Cuts at: 1213 1213
Size: 4193
MluI A'CGCG_T
Cuts at: 3626 3626
Size: 4193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1171 2864 3048 1171
Size: 1693 184 2316
Fragments arranged by size:
2316 1693 184
MscI TGG'CCA
Cuts at: 1954 1954
Size: 4193
MslI CAynn'nnrTG
Cuts at: 560 2182 2309 560
Size: 1622 127 2444
Fragments arranged by size:
2444 1622 127
MspA1I CmG'CkG
Cuts at: 362 1122 1304 1618 1978 2991 3236 362
Size: 760 182 314 360 1013 245 1319
Fragments arranged by size:
1319 1013 760 360 314 245 182
NaeI GCC'GGC
Cuts at: 2374 2374
Size: 4193
NarI GG'CG_CC
Cuts at: 1624 1872 1624
Size: 248 3945
NgoMIV G'CCGG_C
Cuts at: 2372 2372
Size: 4193
NspI r_CATG'y
Cuts at: 554 910 1246 1746 2653 554
Size: 356 336 500 907 2094
Fragments arranged by size:
2094 907 500 356 336
PciI A'CATG_T
Cuts at: 550 2649 550
Size: 2099 2094
PflMI CCAn_nnn'nTGG
Cuts at: 541 541
Size: 4193
PfoI T'CCnGG_A
Cuts at: 289 494 969 289
Size: 205 475 3513
Fragments arranged by size:
3513 475 205
PmeI GTTT'AAAC
Cuts at: 1192 1192
Size: 4193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 4161
PspXI vC'TCGA_Gb
Cuts at: 944 944
Size: 4193
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1029 1061 1029
Size: 32 4161
PstI C_TGCA'G
Cuts at: 1240 1925 1240
Size: 685 3508
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4193
PvuII CAG'CTG
Cuts at: 362 1122 1618 1978 362
Size: 760 496 360 2577
Fragments arranged by size:
2577 760 496 360
RsrII CG'GwC_CG
Cuts at: 2388 2388
Size: 4193
SacI G_AGCT'C
Cuts at: 1207 1207
Size: 4193
SalI G'TCGA_C
Cuts at: 1230 1230
Size: 4193
SapI GCTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
SbfI CC_TGCA'GG
Cuts at: 1240 1240
Size: 4193
ScaI AGT'ACT
Cuts at: 3710 3710
Size: 4193
SfcI C'TryA_G
Cuts at: 33 1236 1921 2914 3105 33
Size: 1203 685 993 191 1121
Fragments arranged by size:
1203 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1625 1873 1625
Size: 248 3945
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
SmaI CCC'GGG
Cuts at: 1215 1215
Size: 4193
SmlI C'TyrA_G
Cuts at: 944 1104 1348 2263 2755 3017 944
Size: 160 244 915 492 262 2120
Fragments arranged by size:
2120 915 492 262 244 160
SpeI A'CTAG_T
Cuts at: 67 882 67
Size: 815 3378
SphI G_CATG'C
Cuts at: 1246 1746 1246
Size: 500 3693
StuI AGG'CCT
Cuts at: 1102 1102
Size: 4193
StyI C'CwwG_G
Cuts at: 1327 1681 1327
Size: 354 3839
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1776 1909 2368 2577 1776
Size: 133 459 209 3392
Fragments arranged by size:
3392 459 209 133
TatI w'GTAC_w
Cuts at: 3708 3708
Size: 4193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 175 207 399 431 1017 1049 3710 3742 175
Size: 32 192 32 586 32 2661 32 626
Fragments arranged by size:
2661 626 586 192 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1990 1990
Size: 4193
XbaI T'CTAG_A
Cuts at: 1224 1224
Size: 4193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1688 1688
Size: 4193
XhoI C'TCGA_G
Cuts at: 944 944
Size: 4193
XmaI C'CCGG_G
Cuts at: 1213 1213
Size: 4193
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI