Restriction map for
pF1KB6512
(Circular) (Six-base) MAPSORT of: pF1KB6512.seq Check: 4063 from: 1 to: 4265
pF1KB6512 4265 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 168 1109 1303 168
Size: 941 194 3130
Fragments arranged by size:
3130 941 194
Acc65I G'GTAC_C
Cuts at: 1281 1281
Size: 4265
AclI AA'CG_TT
Cuts at: 4105 4105
Size: 4265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 977 2090 2522 3269 3537 626
Size: 351 1113 432 747 268 1354
Fragments arranged by size:
1354 1113 747 432 351 268
AflIII A'CryG_T
Cuts at: 1617 2721 3698 1617
Size: 1104 977 2184
Fragments arranged by size:
2184 1104 977
AgeI A'CCGG_T
Cuts at: 2653 2653
Size: 4265
AhdI GACnn_n'nnGTC
Cuts at: 537 927 537
Size: 390 3875
AlwNI CAG_nnn'CTG
Cuts at: 284 541 1570 3137 284
Size: 257 1029 1567 1412
Fragments arranged by size:
1567 1412 1029 257
ApaLI G'TGCA_C
Cuts at: 3035 3035
Size: 4265
ApoI r'AATT_y
Cuts at: 1269 3618 3874 1269
Size: 2349 256 1660
Fragments arranged by size:
2349 1660 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4265
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
AvaI C'yCGr_G
Cuts at: 1285 1285
Size: 4265
BamHI G'GATC_C
Cuts at: 409 970 1290 409
Size: 561 320 3384
Fragments arranged by size:
3384 561 320
BanI G'GyrC_C
Cuts at: 1281 1695 1943 1978 1281
Size: 414 248 35 3568
Fragments arranged by size:
3568 414 248 35
BanII G_rGCy'C
Cuts at: 906 1188 1279 2309 906
Size: 282 91 1030 2862
Fragments arranged by size:
2862 1030 282 91
BbeI G_GCGC'C
Cuts at: 1699 1947 1699
Size: 248 4017
BbsI GAAGACnn'nnnn_
Cuts at: 1065 1065
Size: 4265
BbvCI CC'TCA_GC
Cuts at: 845 1205 845
Size: 360 3905
BciVI GTATCCnnnnn_n'
Cuts at: 2163 2924 4265 2163
Size: 761 1341 2163
Fragments arranged by size:
2163 1341 761
BclI T'GATC_A
Cuts at: 1785 1785
Size: 4265
BglII A'GATC_T
Cuts at: 1780 3817 1780
Size: 2037 2228
BlpI GC'TnA_GC
Cuts at: 1377 1377
Size: 4265
Bme1580I G_kGCm'C
Cuts at: 291 1890 1983 3039 291
Size: 1599 93 1056 1517
Fragments arranged by size:
1599 1517 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 856 1191 1477 1558 1893 4037 856
Size: 335 286 81 335 2144 1084
Fragments arranged by size:
2144 1084 335 335 286 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 843 2632 843
Size: 1789 2476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 917 1021 1026 1172 235
Size: 682 104 5 146 3328
Fragments arranged by size:
3328 682 146 104 5
Bpu10I CC'TnA_GC
Cuts at: 845 1205 1214 845
Size: 360 9 3896
Fragments arranged by size:
3896 360 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1441 2320 2812 3110 1441
Size: 879 492 298 2596
Fragments arranged by size:
2596 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3969 3969
Size: 4265
BsaAI yAC'GTr
Cuts at: 1618 1618
Size: 4265
BsaBI GATnn'nnATC
Cuts at: 6 1779 3816 6
Size: 1773 2037 455
Fragments arranged by size:
2037 1773 455
BsaHI Gr'CG_yC
Cuts at: 1696 1944 2646 4142 1696
Size: 248 702 1496 1819
Fragments arranged by size:
1819 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1454 1975 2653 2927 3074 3485 1454
Size: 521 678 274 147 411 2234
Fragments arranged by size:
2234 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 506 536 999 1029 506
Size: 30 463 30 3742
Fragments arranged by size:
3742 463 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 862 862
Size: 4265
BseYI C'CCAG_C
Cuts at: 1247 1690 2448 3025 3521 1247
Size: 443 758 577 496 1991
Fragments arranged by size:
1991 758 577 496 443
BsiEI CG_ry'CG
Cuts at: 104 1807 1853 3061 104
Size: 1703 46 1208 1308
Fragments arranged by size:
1703 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1279 2057 2247 3039 1279
Size: 778 190 792 2505
Fragments arranged by size:
2505 792 778 190
BsmI GAATG_Cn'
Cuts at: 349 349
Size: 4265
Bsp1286I G_dGCh'C
Cuts at: 291 906 1188 1279 1890 1983 2057 2247 2309
Size: 615 282 91 611 93 74 190 62
Cuts at: 2309 3039 291
Size: 730 1517
Fragments arranged by size:
1517 730 615 611 282 190 93 91
74 62
BspEI T'CCGG_A
Cuts at: 3485 3485
Size: 4265
BspHI T'CATG_A
Cuts at: 3441 3441
Size: 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1315 1529 1831 2212 1315
Size: 214 302 381 3368
Fragments arranged by size:
3368 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2557 2611 4095 4260 50
Size: 2507 54 1484 165 55
Fragments arranged by size:
2507 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2177 2177
Size: 4265
BsrFI r'CCGG_y
Cuts at: 2263 2444 2653 2263
Size: 181 209 3875
Fragments arranged by size:
3875 209 181
BsrGI T'GTAC_A
Cuts at: 524 524
Size: 4265
BssSI C'ACGA_G
Cuts at: 2536 2894 2536
Size: 358 3907
BstAPI GCAn_nnn'nTGC
Cuts at: 550 1642 550
Size: 1092 3173
BstBI TT'CG_AA
Cuts at: 2626 2626
Size: 4265
BstXI CCAn_nnnn'nTGG
Cuts at: 498 898 498
Size: 400 3865
BstYI r'GATC_y
Cuts at: 1 176 409 970 1290 1757 1780 2115 2361
Size: 175 233 561 320 467 23 335 246
Cuts at: 2361 3373 3459 3471 3817 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 561 467 449 346 335 320 246
233 175 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4265
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2215 2396 2559 2215
Size: 181 163 3921
Fragments arranged by size:
3921 181 163
BtsI GCAGTG_nn'
Cuts at: 1508 1508
Size: 4265
DraI TTT'AAA
Cuts at: 1264 1264
Size: 4265
DraIII CAC_nnn'GTG
Cuts at: 1017 1017
Size: 4265
DrdI GACnn_nn'nnGTC
Cuts at: 1971 2829 1971
Size: 858 3407
EaeI y'GGCC_r
Cuts at: 1850 2024 2415 2442 3787 1850
Size: 174 391 27 1345 2328
Fragments arranged by size:
2328 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1850 1850
Size: 4265
EarI CTCTTCn'nnn_
Cuts at: 2288 2498 2288
Size: 210 4055
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2473 2783 2929 4075 2473
Size: 310 146 1146 2663
Fragments arranged by size:
2663 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1277 1277
Size: 4265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 626 917 977 1021 1026 1172 2090 2522
Size: 391 291 60 44 5 146 918 432
Cuts at: 2522 3269 3537 235
Size: 747 268 963
Fragments arranged by size:
963 918 747 432 391 291 268 146
60 44 5
EcoO109I rG'GnC_Cy
Cuts at: 281 1404 281
Size: 1123 3142
EcoRI G'AATT_C
Cuts at: 1269 1269
Size: 4265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 658 2284 658
Size: 1626 2639
FspI TGC'GCA
Cuts at: 2046 2046
Size: 4265
HaeII r_GCGC'y
Cuts at: 1699 1947 2969 3948 1699
Size: 248 1022 979 2016
Fragments arranged by size:
2016 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 156 161 188 193 414 446 523 555 913
Size: 5 27 5 221 32 77 32 358
Cuts at: 913 931 945 963 2107 2137 2139 2169 156
Size: 18 14 18 1144 30 2 30 2252
Fragments arranged by size:
2252 1144 358 221 77 32 32 30
30 27 18 18 14 5 5 2
HincII GTy'rAC
Cuts at: 1304 1304
Size: 4265
KasI G'GCGC_C
Cuts at: 1695 1943 1695
Size: 248 4017
KpnI G_GTAC'C
Cuts at: 1285 1285
Size: 4265
MluI A'CGCG_T
Cuts at: 3698 3698
Size: 4265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2936 3120 2936
Size: 184 4081
MscI TGG'CCA
Cuts at: 2026 2026
Size: 4265
MslI CAynn'nnrTG
Cuts at: 395 496 896 2254 2381 395
Size: 101 400 1358 127 2279
Fragments arranged by size:
2279 1358 400 127 101
MspA1I CmG'CkG
Cuts at: 937 977 1376 1690 2050 3063 3308 937
Size: 40 399 314 360 1013 245 1894
Fragments arranged by size:
1894 1013 399 360 314 245 40
NaeI GCC'GGC
Cuts at: 2446 2446
Size: 4265
NarI GG'CG_CC
Cuts at: 1696 1944 1696
Size: 248 4017
NcoI C'CATG_G
Cuts at: 107 107
Size: 4265
NgoMIV G'CCGG_C
Cuts at: 2444 2444
Size: 4265
NspI r_CATG'y
Cuts at: 1318 1818 2725 1318
Size: 500 907 2858
Fragments arranged by size:
2858 907 500
PciI A'CATG_T
Cuts at: 2721 2721
Size: 4265
PflMI CCAn_nnn'nTGG
Cuts at: 442 442
Size: 4265
PmeI GTTT'AAAC
Cuts at: 1264 1264
Size: 4265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 3404
Size: 32 4233
PstI C_TGCA'G
Cuts at: 1312 1997 1312
Size: 685 3580
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4265
PvuII CAG'CTG
Cuts at: 937 977 1690 2050 937
Size: 40 713 360 3152
Fragments arranged by size:
3152 713 360 40
RsrII CG'GwC_CG
Cuts at: 2460 2460
Size: 4265
SacI G_AGCT'C
Cuts at: 1279 1279
Size: 4265
SalI G'TCGA_C
Cuts at: 1302 1302
Size: 4265
SapI GCTCTTCn'nnn_
Cuts at: 2288 2498 2288
Size: 210 4055
SbfI CC_TGCA'GG
Cuts at: 1312 1312
Size: 4265
ScaI AGT'ACT
Cuts at: 3782 3782
Size: 4265
SexAI A'CCwGG_T
Cuts at: 435 923 435
Size: 488 3777
SfcI C'TryA_G
Cuts at: 33 1228 1308 1993 2986 3177 33
Size: 1195 80 685 993 191 1121
Fragments arranged by size:
1195 1121 993 685 191 80
SfoI GGC'GCC
Cuts at: 1697 1945 1697
Size: 248 4017
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4265
SmaI CCC'GGG
Cuts at: 1287 1287
Size: 4265
SmlI C'TyrA_G
Cuts at: 1420 2335 2827 3089 1420
Size: 915 492 262 2596
Fragments arranged by size:
2596 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4265
SphI G_CATG'C
Cuts at: 1318 1818 1318
Size: 500 3765
StuI AGG'CCT
Cuts at: 1130 1130
Size: 4265
StyI C'CwwG_G
Cuts at: 107 1399 1753 107
Size: 1292 354 2619
Fragments arranged by size:
2619 1292 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1180 1848 1981 2440 2649 1180
Size: 668 133 459 209 2796
Fragments arranged by size:
2796 668 459 209 133
TatI w'GTAC_w
Cuts at: 524 3780 524
Size: 3256 1009
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 506 538 999 1031 3782 3814 506
Size: 32 461 32 2751 32 957
Fragments arranged by size:
2751 957 461 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2062 2062
Size: 4265
XbaI T'CTAG_A
Cuts at: 1296 1296
Size: 4265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1083 1760 1083
Size: 677 3588
XmaI C'CCGG_G
Cuts at: 1285 1285
Size: 4265
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsgI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI