Restriction map for
pF1KB6503
(Circular) (Six-base) MAPSORT of: pF1KB6503.seq Check: 4138 from: 1 to: 4187
pF1KB6503 4187 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1225 1225
Size: 4187
Acc65I G'GTAC_C
Cuts at: 773 1203 773
Size: 430 3757
AclI AA'CG_TT
Cuts at: 4027 4027
Size: 4187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 2012 2444 3191 3459 128
Size: 1884 432 747 268 856
Fragments arranged by size:
1884 856 747 432 268
AflIII A'CryG_T
Cuts at: 965 1539 2643 3620 965
Size: 574 1104 977 1532
Fragments arranged by size:
1532 1104 977 574
AgeI A'CCGG_T
Cuts at: 2575 2575
Size: 4187
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 952 984 952
Size: 32 4155
AlwNI CAG_nnn'CTG
Cuts at: 533 604 864 1492 3059 533
Size: 71 260 628 1567 1661
Fragments arranged by size:
1661 1567 628 260 71
ApaLI G'TGCA_C
Cuts at: 416 2957 416
Size: 2541 1646
ApoI r'AATT_y
Cuts at: 1191 3540 3796 1191
Size: 2349 256 1582
Fragments arranged by size:
2349 1582 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
AvaI C'yCGr_G
Cuts at: 1207 1207
Size: 4187
BamHI G'GATC_C
Cuts at: 1212 1212
Size: 4187
BanI G'GyrC_C
Cuts at: 773 1203 1617 1865 1900 773
Size: 430 414 248 35 3060
Fragments arranged by size:
3060 430 414 248 35
BanII G_rGCy'C
Cuts at: 917 1201 2231 917
Size: 284 1030 2873
Fragments arranged by size:
2873 1030 284
BbeI G_GCGC'C
Cuts at: 1621 1869 1621
Size: 248 3939
BbsI GAAGACnn'nnnn_
Cuts at: 747 1009 747
Size: 262 3925
BbvCI CC'TCA_GC
Cuts at: 113 113
Size: 4187
BciVI GTATCCnnnnn_n'
Cuts at: 991 2085 2846 4187 991
Size: 1094 761 1341 991
Fragments arranged by size:
1341 1094 991 761
BclI T'GATC_A
Cuts at: 1707 1707
Size: 4187
BfrBI ATG'CAT
Cuts at: 389 389
Size: 4187
BglII A'GATC_T
Cuts at: 1702 3739 1702
Size: 2037 2150
BlpI GC'TnA_GC
Cuts at: 1299 1299
Size: 4187
Bme1580I G_kGCm'C
Cuts at: 413 420 1812 1905 2961 413
Size: 7 1392 93 1056 1639
Fragments arranged by size:
1639 1392 1056 93 7
BmgBI CAC'GTC
Cuts at: 1175 1175
Size: 4187
BmrI ACTGGGnnnn_n'
Cuts at: 1399 1480 1815 3959 1399
Size: 81 335 2144 1627
Fragments arranged by size:
2144 1627 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 4187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 473 781 931 994 1132 143
Size: 330 308 150 63 138 3198
Fragments arranged by size:
3198 330 308 150 138 63
Bpu10I CC'TnA_GC
Cuts at: 113 113
Size: 4187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 1363 2242 2734 3032 799
Size: 564 879 492 298 1954
Fragments arranged by size:
1954 879 564 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1087 3891 1087
Size: 2804 1383
BsaAI yAC'GTr
Cuts at: 1540 1540
Size: 4187
BsaBI GATnn'nnATC
Cuts at: 6 1701 3738 6
Size: 1695 2037 455
Fragments arranged by size:
2037 1695 455
BsaHI Gr'CG_yC
Cuts at: 1618 1866 2568 4064 1618
Size: 248 702 1496 1741
Fragments arranged by size:
1741 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1376 1897 2575 2849 2996 3407 1376
Size: 521 678 274 147 411 2156
Fragments arranged by size:
2156 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 301 331 301
Size: 30 4157
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 476 163
Size: 313 3874
BseYI C'CCAG_C
Cuts at: 1612 2370 2947 3443 1612
Size: 758 577 496 2356
Fragments arranged by size:
2356 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1729 1775 2983 104
Size: 1625 46 1208 1308
Fragments arranged by size:
1625 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 420 728 1025 1201 1979 2169 2961 420
Size: 308 297 176 778 190 792 1646
Fragments arranged by size:
1646 792 778 308 297 190 176
BsmI GAATG_Cn'
Cuts at: 391 391
Size: 4187
Bsp1286I G_dGCh'C
Cuts at: 413 420 728 917 1025 1201 1812 1905 1979
Size: 7 308 189 108 176 611 93 74
Cuts at: 1979 2169 2231 2961 413
Size: 190 62 730 1639
Fragments arranged by size:
1639 730 611 308 190 189 176 108
93 74 62 7
BspEI T'CCGG_A
Cuts at: 3407 3407
Size: 4187
BspHI T'CATG_A
Cuts at: 339 3363 339
Size: 3024 1163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1237 1451 1753 2134 1237
Size: 214 302 381 3290
Fragments arranged by size:
3290 381 302 214
BsrBI CCG'CTC
Cuts at: 50 123 2479 2533 4017 4182 50
Size: 73 2356 54 1484 165 55
Fragments arranged by size:
2356 1484 165 73 55 54
BsrDI GCAATG_nn'
Cuts at: 2099 2099
Size: 4187
BsrFI r'CCGG_y
Cuts at: 823 2185 2366 2575 823
Size: 1362 181 209 2435
Fragments arranged by size:
2435 1362 209 181
BssSI C'ACGA_G
Cuts at: 2458 2816 2458
Size: 358 3829
BstAPI GCAn_nnn'nTGC
Cuts at: 1564 1564
Size: 4187
BstBI TT'CG_AA
Cuts at: 2548 2548
Size: 4187
BstYI r'GATC_y
Cuts at: 1 194 1212 1679 1702 2037 2283 3295 3381
Size: 193 1018 467 23 335 246 1012 86
Cuts at: 3381 3393 3739 1
Size: 12 346 449
Fragments arranged by size:
1018 1012 467 449 346 335 246 193
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 611 611
Size: 4187
BtgI C'CryG_G
Cuts at: 107 107
Size: 4187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 395 2137 2318 2481 395
Size: 1742 181 163 2101
Fragments arranged by size:
2101 1742 181 163
BtsI GCAGTG_nn'
Cuts at: 1430 1430
Size: 4187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 639 674 639
Size: 35 4152
DraI TTT'AAA
Cuts at: 1186 1186
Size: 4187
DraIII CAC_nnn'GTG
Cuts at: 927 927
Size: 4187
DrdI GACnn_nn'nnGTC
Cuts at: 1893 2751 1893
Size: 858 3329
EaeI y'GGCC_r
Cuts at: 572 1772 1946 2337 2364 3709 572
Size: 1200 174 391 27 1345 1050
Fragments arranged by size:
1345 1200 1050 391 174 27
EagI C'GGCC_G
Cuts at: 1772 1772
Size: 4187
EarI CTCTTCn'nnn_
Cuts at: 455 2210 2420 455
Size: 1755 210 2222
Fragments arranged by size:
2222 1755 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2395 2705 2851 3997 2395
Size: 310 146 1146 2585
Fragments arranged by size:
2585 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1199 1199
Size: 4187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 143 473 781 931 994 1132 2012 2444
Size: 15 330 308 150 63 138 880 432
Cuts at: 2444 3191 3459 128
Size: 747 268 856
Fragments arranged by size:
880 856 747 432 330 308 268 150
138 63 15
EcoO109I rG'GnC_Cy
Cuts at: 153 530 1326 153
Size: 377 796 3014
Fragments arranged by size:
3014 796 377
EcoRI G'AATT_C
Cuts at: 1191 1191
Size: 4187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 318 2206 318
Size: 1888 2299
FspI TGC'GCA
Cuts at: 1968 1968
Size: 4187
HaeII r_GCGC'y
Cuts at: 1052 1621 1869 2891 3870 1052
Size: 569 248 1022 979 1369
Fragments arranged by size:
1369 1022 979 569 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 487 519 2029 2059 2061 2091 487
Size: 32 1510 30 2 30 2583
Fragments arranged by size:
2583 1510 32 30 30 2
HincII GTy'rAC
Cuts at: 1226 1226
Size: 4187
KasI G'GCGC_C
Cuts at: 1617 1865 1617
Size: 248 3939
KpnI G_GTAC'C
Cuts at: 777 1207 777
Size: 430 3757
MluI A'CGCG_T
Cuts at: 3620 3620
Size: 4187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1052 2858 3042 1052
Size: 1806 184 2197
Fragments arranged by size:
2197 1806 184
MscI TGG'CCA
Cuts at: 574 1948 574
Size: 1374 2813
MslI CAynn'nnrTG
Cuts at: 1090 2176 2303 1090
Size: 1086 127 2974
Fragments arranged by size:
2974 1086 127
MspA1I CmG'CkG
Cuts at: 601 891 1035 1298 1612 1972 2985 3230 601
Size: 290 144 263 314 360 1013 245 1558
Fragments arranged by size:
1558 1013 360 314 290 263 245 144
NaeI GCC'GGC
Cuts at: 825 2368 825
Size: 1543 2644
NarI GG'CG_CC
Cuts at: 1618 1866 1618
Size: 248 3939
NcoI C'CATG_G
Cuts at: 107 107
Size: 4187
NgoMIV G'CCGG_C
Cuts at: 823 2366 823
Size: 1543 2644
NsiI A_TGCA'T
Cuts at: 391 391
Size: 4187
NspI r_CATG'y
Cuts at: 969 1095 1240 1740 2647 969
Size: 126 145 500 907 2509
Fragments arranged by size:
2509 907 500 145 126
PciI A'CATG_T
Cuts at: 965 2643 965
Size: 1678 2509
PfoI T'CCnGG_A
Cuts at: 971 971
Size: 4187
PmeI GTTT'AAAC
Cuts at: 1186 1186
Size: 4187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 4155
PpuMI rG'GwC_Cy
Cuts at: 530 530
Size: 4187
PstI C_TGCA'G
Cuts at: 1234 1919 1234
Size: 685 3502
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4187
PvuII CAG'CTG
Cuts at: 601 891 1035 1612 1972 601
Size: 290 144 577 360 2816
Fragments arranged by size:
2816 577 360 290 144
RsrII CG'GwC_CG
Cuts at: 2382 2382
Size: 4187
SacI G_AGCT'C
Cuts at: 1201 1201
Size: 4187
SalI G'TCGA_C
Cuts at: 1224 1224
Size: 4187
SapI GCTCTTCn'nnn_
Cuts at: 455 2210 2420 455
Size: 1755 210 2222
Fragments arranged by size:
2222 1755 210
SbfI CC_TGCA'GG
Cuts at: 1234 1234
Size: 4187
ScaI AGT'ACT
Cuts at: 3704 3704
Size: 4187
SexAI A'CCwGG_T
Cuts at: 532 532
Size: 4187
SfcI C'TryA_G
Cuts at: 33 1230 1915 2908 3099 33
Size: 1197 685 993 191 1121
Fragments arranged by size:
1197 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1619 1867 1619
Size: 248 3939
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
SmaI CCC'GGG
Cuts at: 1209 1209
Size: 4187
SmlI C'TyrA_G
Cuts at: 778 1342 2257 2749 3011 778
Size: 564 915 492 262 1954
Fragments arranged by size:
1954 915 564 492 262
SpeI A'CTAG_T
Cuts at: 67 441 67
Size: 374 3813
SphI G_CATG'C
Cuts at: 1240 1740 1240
Size: 500 3687
StyI C'CwwG_G
Cuts at: 107 1321 1675 107
Size: 1214 354 2619
Fragments arranged by size:
2619 1214 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 917 1770 1903 2362 2571 917
Size: 853 133 459 209 2533
Fragments arranged by size:
2533 853 459 209 133
TatI w'GTAC_w
Cuts at: 308 3702 308
Size: 3394 793
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3704 3736 3704
Size: 32 4155
Tth111I GACn'n_nGTC
Cuts at: 1984 1984
Size: 4187
XbaI T'CTAG_A
Cuts at: 1218 1218
Size: 4187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 924 1682 924
Size: 758 3429
XmaI C'CCGG_G
Cuts at: 1207 1207
Size: 4187
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BcgI BglI BmtI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI