Restriction map for
pF1KB6490
(Circular) (Six-base) MAPSORT of: pF1KB6490.seq Check: 4807 from: 1 to: 4184
pF1KB6490 4184 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 469 469
Size: 4184
AccI GT'mk_AC
Cuts at: 485 803 1222 485
Size: 318 419 3447
Fragments arranged by size:
3447 419 318
Acc65I G'GTAC_C
Cuts at: 1200 1200
Size: 4184
AclI AA'CG_TT
Cuts at: 4024 4024
Size: 4184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 2009 2441 3188 3456 250
Size: 1759 432 747 268 978
Fragments arranged by size:
1759 978 747 432 268
AflIII A'CryG_T
Cuts at: 1536 2640 3617 1536
Size: 1104 977 2103
Fragments arranged by size:
2103 1104 977
AgeI A'CCGG_T
Cuts at: 2572 2572
Size: 4184
AleI CACnn'nnGTG
Cuts at: 400 400
Size: 4184
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 110 142 110
Size: 32 4152
AlwNI CAG_nnn'CTG
Cuts at: 1152 1489 3056 1152
Size: 337 1567 2280
Fragments arranged by size:
2280 1567 337
ApaLI G'TGCA_C
Cuts at: 2954 2954
Size: 4184
ApoI r'AATT_y
Cuts at: 1188 3537 3793 1188
Size: 2349 256 1579
Fragments arranged by size:
2349 1579 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4184
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
AvaI C'yCGr_G
Cuts at: 1204 1204
Size: 4184
BamHI G'GATC_C
Cuts at: 143 1209 143
Size: 1066 3118
BanI G'GyrC_C
Cuts at: 1200 1614 1862 1897 1200
Size: 414 248 35 3487
Fragments arranged by size:
3487 414 248 35
BanII G_rGCy'C
Cuts at: 454 1198 2228 454
Size: 744 1030 2410
Fragments arranged by size:
2410 1030 744
BbeI G_GCGC'C
Cuts at: 1618 1866 1618
Size: 248 3936
BbsI GAAGACnn'nnnn_
Cuts at: 1062 1062
Size: 4184
BbvCI CC'TCA_GC
Cuts at: 994 994
Size: 4184
BciVI GTATCCnnnnn_n'
Cuts at: 369 2082 2843 4184 369
Size: 1713 761 1341 369
Fragments arranged by size:
1713 1341 761 369
BclI T'GATC_A
Cuts at: 1704 1704
Size: 4184
BglI GCCn_nnn'nGGC
Cuts at: 458 458
Size: 4184
BglII A'GATC_T
Cuts at: 1699 3736 1699
Size: 2037 2147
BlpI GC'TnA_GC
Cuts at: 1296 1296
Size: 4184
Bme1580I G_kGCm'C
Cuts at: 407 475 907 1109 1809 1902 2958 407
Size: 68 432 202 700 93 1056 1633
Fragments arranged by size:
1633 1056 700 432 202 93 68
BmgBI CAC'GTC
Cuts at: 1029 1029
Size: 4184
BmrI ACTGGGnnnn_n'
Cuts at: 805 1396 1477 1812 3956 805
Size: 591 81 335 2144 1033
Fragments arranged by size:
2144 1033 591 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 334 2551 334
Size: 2217 1967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 677 841 921 148
Size: 529 164 80 3411
Fragments arranged by size:
3411 529 164 80
Bpu10I CC'TnA_GC
Cuts at: 994 994
Size: 4184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 772 877 1360 2239 2731 3029 709
Size: 63 105 483 879 492 298 1864
Fragments arranged by size:
1864 879 492 483 298 105 63
BsaI GGTCTCn'nnnn_
Cuts at: 3888 3888
Size: 4184
BsaAI yAC'GTr
Cuts at: 492 1537 492
Size: 1045 3139
BsaBI GATnn'nnATC
Cuts at: 6 1698 3735 6
Size: 1692 2037 455
Fragments arranged by size:
2037 1692 455
BsaHI Gr'CG_yC
Cuts at: 1615 1863 2565 4061 1615
Size: 248 702 1496 1738
Fragments arranged by size:
1738 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1373 1894 2572 2846 2993 3404 1373
Size: 521 678 274 147 411 2153
Fragments arranged by size:
2153 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 146 166 176 196 146
Size: 20 10 20 4134
Fragments arranged by size:
4134 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 880 880
Size: 4184
BseYI C'CCAG_C
Cuts at: 1609 2367 2944 3440 1609
Size: 758 577 496 2353
Fragments arranged by size:
2353 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 1009 613
Size: 396 3788
BsiEI CG_ry'CG
Cuts at: 104 1726 1772 2980 104
Size: 1622 46 1208 1308
Fragments arranged by size:
1622 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1198 1976 2166 2958 1198
Size: 778 190 792 2424
Fragments arranged by size:
2424 792 778 190
BsmI GAATG_Cn'
Cuts at: 234 234
Size: 4184
Bsp1286I G_dGCh'C
Cuts at: 407 454 475 907 1109 1198 1809 1902 1976
Size: 47 21 432 202 89 611 93 74
Cuts at: 1976 2166 2228 2958 407
Size: 190 62 730 1633
Fragments arranged by size:
1633 730 611 432 202 190 93 89
74 62 47 21
BspEI T'CCGG_A
Cuts at: 3404 3404
Size: 4184
BspHI T'CATG_A
Cuts at: 3360 3360
Size: 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 469 1234 1448 1750 2131 469
Size: 765 214 302 381 2522
Fragments arranged by size:
2522 765 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2476 2530 4014 4179 50
Size: 2426 54 1484 165 55
Fragments arranged by size:
2426 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 671 2096 671
Size: 1425 2759
BsrFI r'CCGG_y
Cuts at: 2182 2363 2572 2182
Size: 181 209 3794
Fragments arranged by size:
3794 209 181
BsrGI T'GTAC_A
Cuts at: 944 944
Size: 4184
BssSI C'ACGA_G
Cuts at: 2455 2813 2455
Size: 358 3826
BstAPI GCAn_nnn'nTGC
Cuts at: 1561 1561
Size: 4184
BstBI TT'CG_AA
Cuts at: 2545 2545
Size: 4184
BstXI CCAn_nnnn'nTGG
Cuts at: 139 365 139
Size: 226 3958
BstYI r'GATC_y
Cuts at: 1 143 1209 1676 1699 2034 2280 3292 3378
Size: 142 1066 467 23 335 246 1012 86
Cuts at: 3378 3390 3736 1
Size: 12 346 449
Fragments arranged by size:
1066 1012 467 449 346 335 246 142
86 23 12
BstZ17I GTA'TAC
Cuts at: 486 486
Size: 4184
Bsu36I CC'TnA_GG
Cuts at: 223 223
Size: 4184
BtgI C'CryG_G
Cuts at: 107 467 107
Size: 360 3824
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2134 2315 2478 2134
Size: 181 163 3840
Fragments arranged by size:
3840 181 163
BtsI GCAGTG_nn'
Cuts at: 1427 1427
Size: 4184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 861 896 861
Size: 35 4149
DraI TTT'AAA
Cuts at: 1183 1183
Size: 4184
DrdI GACnn_nn'nnGTC
Cuts at: 1890 2748 1890
Size: 858 3326
EaeI y'GGCC_r
Cuts at: 459 1769 1943 2334 2361 3706 459
Size: 1310 174 391 27 1345 937
Fragments arranged by size:
1345 1310 937 391 174 27
EagI C'GGCC_G
Cuts at: 1769 1769
Size: 4184
EarI CTCTTCn'nnn_
Cuts at: 250 2207 2417 250
Size: 1957 210 2017
Fragments arranged by size:
2017 1957 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 979 2392 2702 2848 3994 979
Size: 1413 310 146 1146 1169
Fragments arranged by size:
1413 1169 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1196 1196
Size: 4184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 250 677 841 921 2009 2441 3188 3456
Size: 102 427 164 80 1088 432 747 268
Cuts at: 3456 148
Size: 876
Fragments arranged by size:
1088 876 747 432 427 268 164 102
80
EcoNI CCTnn'n_nnAGG
Cuts at: 1144 1144
Size: 4184
EcoO109I rG'GnC_Cy
Cuts at: 1149 1323 1149
Size: 174 4010
EcoRI G'AATT_C
Cuts at: 1188 1188
Size: 4184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 4184
FspI TGC'GCA
Cuts at: 1965 1965
Size: 4184
HaeII r_GCGC'y
Cuts at: 1618 1866 2888 3867 1618
Size: 248 1022 979 1935
Fragments arranged by size:
1935 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2026 2056 2058 2088 2026
Size: 30 2 30 4122
Fragments arranged by size:
4122 30 30 2
HincII GTy'rAC
Cuts at: 152 1223 152
Size: 1071 3113
KasI G'GCGC_C
Cuts at: 1614 1862 1614
Size: 248 3936
KpnI G_GTAC'C
Cuts at: 1204 1204
Size: 4184
MluI A'CGCG_T
Cuts at: 3617 3617
Size: 4184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2855 3039 2855
Size: 184 4000
MscI TGG'CCA
Cuts at: 461 1945 461
Size: 1484 2700
MslI CAynn'nnrTG
Cuts at: 202 363 400 2173 2300 202
Size: 161 37 1773 127 2086
Fragments arranged by size:
2086 1773 161 127 37
MspA1I CmG'CkG
Cuts at: 597 1295 1609 1969 2982 3227 597
Size: 698 314 360 1013 245 1554
Fragments arranged by size:
1554 1013 698 360 314 245
NaeI GCC'GGC
Cuts at: 2365 2365
Size: 4184
NarI GG'CG_CC
Cuts at: 1615 1863 1615
Size: 248 3936
NcoI C'CATG_G
Cuts at: 107 467 107
Size: 360 3824
NgoMIV G'CCGG_C
Cuts at: 2363 2363
Size: 4184
NspI r_CATG'y
Cuts at: 945 1237 1737 2644 945
Size: 292 500 907 2485
Fragments arranged by size:
2485 907 500 292
PciI A'CATG_T
Cuts at: 2640 2640
Size: 4184
PflMI CCAn_nnn'nTGG
Cuts at: 468 1152 468
Size: 684 3500
PmeI GTTT'AAAC
Cuts at: 1183 1183
Size: 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 4152
PpuMI rG'GwC_Cy
Cuts at: 1149 1149
Size: 4184
PstI C_TGCA'G
Cuts at: 1231 1916 1231
Size: 685 3499
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4184
PvuII CAG'CTG
Cuts at: 597 1609 1969 597
Size: 1012 360 2812
Fragments arranged by size:
2812 1012 360
RsrII CG'GwC_CG
Cuts at: 2379 2379
Size: 4184
SacI G_AGCT'C
Cuts at: 1198 1198
Size: 4184
SalI G'TCGA_C
Cuts at: 1221 1221
Size: 4184
SapI GCTCTTCn'nnn_
Cuts at: 2207 2417 2207
Size: 210 3974
SbfI CC_TGCA'GG
Cuts at: 1231 1231
Size: 4184
ScaI AGT'ACT
Cuts at: 3701 3701
Size: 4184
SfcI C'TryA_G
Cuts at: 33 387 543 1227 1912 2905 3096 33
Size: 354 156 684 685 993 191 1121
Fragments arranged by size:
1121 993 685 684 354 191 156
SfoI GGC'GCC
Cuts at: 1616 1864 1616
Size: 248 3936
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
SmaI CCC'GGG
Cuts at: 1206 1206
Size: 4184
SmlI C'TyrA_G
Cuts at: 724 787 892 1339 2254 2746 3008 724
Size: 63 105 447 915 492 262 1900
Fragments arranged by size:
1900 915 492 447 262 105 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4184
SphI G_CATG'C
Cuts at: 1237 1737 1237
Size: 500 3684
SspI AAT'ATT
Cuts at: 733 733
Size: 4184
StyI C'CwwG_G
Cuts at: 107 467 1318 1672 107
Size: 360 851 354 2619
Fragments arranged by size:
2619 851 360 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 787 1767 1900 2359 2568 787
Size: 980 133 459 209 2403
Fragments arranged by size:
2403 980 459 209 133
TatI w'GTAC_w
Cuts at: 944 3699 944
Size: 2755 1429
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 146 178 3701 3733 146
Size: 32 3523 32 597
Fragments arranged by size:
3523 597 32 32
Tth111I GACn'n_nGTC
Cuts at: 353 1981 353
Size: 1628 2556
XbaI T'CTAG_A
Cuts at: 1215 1215
Size: 4184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1679 1679
Size: 4184
XmaI C'CCGG_G
Cuts at: 1204 1204
Size: 4184
XmnI GAAnn'nnTTC
Cuts at: 232 232
Size: 4184
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmBI BssHII BstEII
ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI