Restriction map for
pF1KB6489
(Circular) (Six-base) MAPSORT of: pF1KB6489.seq Check: 5198 from: 1 to: 4100
pF1KB6489 4100 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 136 703 136
Size: 567 3533
AccI GT'mk_AC
Cuts at: 1138 1138
Size: 4100
Acc65I G'GTAC_C
Cuts at: 120 1116 120
Size: 996 3104
AclI AA'CG_TT
Cuts at: 1086 3940 1086
Size: 2854 1246
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 669 1925 2357 3104 3372 466
Size: 203 1256 432 747 268 1194
Fragments arranged by size:
1256 1194 747 432 268 203
AflIII A'CryG_T
Cuts at: 474 511 1452 2556 3533 474
Size: 37 941 1104 977 1041
Fragments arranged by size:
1104 1041 977 941 37
AgeI A'CCGG_T
Cuts at: 2488 2488
Size: 4100
AlwNI CAG_nnn'CTG
Cuts at: 133 883 973 1405 2972 133
Size: 750 90 432 1567 1261
Fragments arranged by size:
1567 1261 750 432 90
ApaLI G'TGCA_C
Cuts at: 2870 2870
Size: 4100
ApoI r'AATT_y
Cuts at: 1104 3453 3709 1104
Size: 2349 256 1495
Fragments arranged by size:
2349 1495 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4100
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
AvaI C'yCGr_G
Cuts at: 1120 1120
Size: 4100
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 276 309 276
Size: 33 4067
BamHI G'GATC_C
Cuts at: 1125 1125
Size: 4100
BanI G'GyrC_C
Cuts at: 120 1116 1530 1778 1813 120
Size: 996 414 248 35 2407
Fragments arranged by size:
2407 996 414 248 35
BanII G_rGCy'C
Cuts at: 1114 2144 1114
Size: 1030 3070
BbeI G_GCGC'C
Cuts at: 1534 1782 1534
Size: 248 3852
BbsI GAAGACnn'nnnn_
Cuts at: 587 587
Size: 4100
BciVI GTATCCnnnnn_n'
Cuts at: 277 349 1998 2759 4100 277
Size: 72 1649 761 1341 277
Fragments arranged by size:
1649 1341 761 277 72
BclI T'GATC_A
Cuts at: 1620 1620
Size: 4100
BglII A'GATC_T
Cuts at: 1072 1615 3652 1072
Size: 543 2037 1520
Fragments arranged by size:
2037 1520 543
BlpI GC'TnA_GC
Cuts at: 1212 1212
Size: 4100
Bme1580I G_kGCm'C
Cuts at: 1725 1818 2874 1725
Size: 93 1056 2951
Fragments arranged by size:
2951 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 299 1312 1393 1728 3872 299
Size: 1013 81 335 2144 527
Fragments arranged by size:
2144 1013 527 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 754
Size: 4100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1276 2155 2647 2945 1276
Size: 879 492 298 2431
Fragments arranged by size:
2431 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 672 3804 672
Size: 3132 968
BsaAI yAC'GTr
Cuts at: 1453 1453
Size: 4100
BsaBI GATnn'nnATC
Cuts at: 6 1614 3651 6
Size: 1608 2037 455
Fragments arranged by size:
2037 1608 455
BsaHI Gr'CG_yC
Cuts at: 1531 1779 2481 3977 1531
Size: 248 702 1496 1654
Fragments arranged by size:
1654 1496 702 248
BsaWI w'CCGG_w
Cuts at: 350 935 1289 1810 2488 2762 2909 3320 350
Size: 585 354 521 678 274 147 411 1130
Fragments arranged by size:
1130 678 585 521 411 354 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 727 757 727
Size: 30 4070
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 185 185
Size: 4100
BseYI C'CCAG_C
Cuts at: 1525 2283 2860 3356 1525
Size: 758 577 496 2269
Fragments arranged by size:
2269 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 813 760
Size: 53 4047
BsiEI CG_ry'CG
Cuts at: 104 1642 1688 2896 104
Size: 1538 46 1208 1308
Fragments arranged by size:
1538 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1114 1892 2082 2874 1114
Size: 778 190 792 2340
Fragments arranged by size:
2340 792 778 190
BsmI GAATG_Cn'
Cuts at: 609 609
Size: 4100
Bsp1286I G_dGCh'C
Cuts at: 1114 1725 1818 1892 2082 2144 2874 1114
Size: 611 93 74 190 62 730 2340
Fragments arranged by size:
2340 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 935 3320 935
Size: 2385 1715
BspHI T'CATG_A
Cuts at: 665 3276 665
Size: 2611 1489
BspMI ACCTGCnnnn'nnnn_
Cuts at: 136 703 1150 1364 1666 2047 136
Size: 567 447 214 302 381 2189
Fragments arranged by size:
2189 567 447 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2392 2446 3930 4095 50
Size: 2342 54 1484 165 55
Fragments arranged by size:
2342 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 968 2012 968
Size: 1044 3056
BsrFI r'CCGG_y
Cuts at: 2098 2279 2488 2098
Size: 181 209 3710
Fragments arranged by size:
3710 209 181
BsrGI T'GTAC_A
Cuts at: 803 803
Size: 4100
BssSI C'ACGA_G
Cuts at: 2371 2729 2371
Size: 358 3742
BstAPI GCAn_nnn'nTGC
Cuts at: 1477 1477
Size: 4100
BstBI TT'CG_AA
Cuts at: 2461 2461
Size: 4100
BstXI CCAn_nnnn'nTGG
Cuts at: 331 401 331
Size: 70 4030
BstYI r'GATC_y
Cuts at: 1 177 1072 1125 1592 1615 1950 2196 3208
Size: 176 895 53 467 23 335 246 1012
Cuts at: 3208 3294 3306 3652 1
Size: 86 12 346 449
Fragments arranged by size:
1012 895 467 449 346 335 246 176
86 53 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2050 2231 2394 2050
Size: 181 163 3756
Fragments arranged by size:
3756 181 163
BtsI GCAGTG_nn'
Cuts at: 748 1343 748
Size: 595 3505
DraI TTT'AAA
Cuts at: 1099 1099
Size: 4100
DrdI GACnn_nn'nnGTC
Cuts at: 1806 2664 1806
Size: 858 3242
EaeI y'GGCC_r
Cuts at: 1685 1859 2250 2277 3622 1685
Size: 174 391 27 1345 2163
Fragments arranged by size:
2163 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1685 1685
Size: 4100
EarI CTCTTCn'nnn_
Cuts at: 2123 2333 2123
Size: 210 3890
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2308 2618 2764 3910 2308
Size: 310 146 1146 2498
Fragments arranged by size:
2498 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1112 1112
Size: 4100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 669 754 1925 2357 3104 3372 466
Size: 203 85 1171 432 747 268 1194
Fragments arranged by size:
1194 1171 747 432 268 203 85
EcoO109I rG'GnC_Cy
Cuts at: 226 623 1239 226
Size: 397 616 3087
Fragments arranged by size:
3087 616 397
EcoRI G'AATT_C
Cuts at: 1104 1104
Size: 4100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 4100
FspI TGC'GCA
Cuts at: 1881 1881
Size: 4100
HaeII r_GCGC'y
Cuts at: 1534 1782 2804 3783 1534
Size: 248 1022 979 1851
Fragments arranged by size:
1851 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 883 898 915 930 1942 1972 1974 2004 883
Size: 15 17 15 1012 30 2 30 2979
Fragments arranged by size:
2979 1012 30 30 17 15 15 2
HincII GTy'rAC
Cuts at: 1139 1139
Size: 4100
KasI G'GCGC_C
Cuts at: 1530 1778 1530
Size: 248 3852
KpnI G_GTAC'C
Cuts at: 124 1120 124
Size: 996 3104
MluI A'CGCG_T
Cuts at: 3533 3533
Size: 4100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 443 941 2771 2955 443
Size: 498 1830 184 1588
Fragments arranged by size:
1830 1588 498 184
MscI TGG'CCA
Cuts at: 1861 1861
Size: 4100
MslI CAynn'nnrTG
Cuts at: 670 2089 2216 670
Size: 1419 127 2554
Fragments arranged by size:
2554 1419 127
MspA1I CmG'CkG
Cuts at: 241 1211 1525 1885 2898 3143 241
Size: 970 314 360 1013 245 1198
Fragments arranged by size:
1198 1013 970 360 314 245
NaeI GCC'GGC
Cuts at: 2281 2281
Size: 4100
NarI GG'CG_CC
Cuts at: 1531 1779 1531
Size: 248 3852
NgoMIV G'CCGG_C
Cuts at: 2279 2279
Size: 4100
NspI r_CATG'y
Cuts at: 478 515 1153 1653 2560 478
Size: 37 638 500 907 2018
Fragments arranged by size:
2018 907 638 500 37
PciI A'CATG_T
Cuts at: 474 511 2556 474
Size: 37 2045 2018
Fragments arranged by size:
2045 2018 37
PflMI CCAn_nnn'nTGG
Cuts at: 256 334 256
Size: 78 4022
PmeI GTTT'AAAC
Cuts at: 1099 1099
Size: 4100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 4068
PpuMI rG'GwC_Cy
Cuts at: 226 226
Size: 4100
PstI C_TGCA'G
Cuts at: 147 1147 1832 147
Size: 1000 685 2415
Fragments arranged by size:
2415 1000 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4100
PvuII CAG'CTG
Cuts at: 241 1525 1885 241
Size: 1284 360 2456
Fragments arranged by size:
2456 1284 360
RsrII CG'GwC_CG
Cuts at: 2295 2295
Size: 4100
SacI G_AGCT'C
Cuts at: 1114 1114
Size: 4100
SalI G'TCGA_C
Cuts at: 1137 1137
Size: 4100
SapI GCTCTTCn'nnn_
Cuts at: 2123 2333 2123
Size: 210 3890
SbfI CC_TGCA'GG
Cuts at: 147 1147 147
Size: 1000 3100
ScaI AGT'ACT
Cuts at: 3617 3617
Size: 4100
SfcI C'TryA_G
Cuts at: 33 143 288 1143 1828 2821 3012 33
Size: 110 145 855 685 993 191 1121
Fragments arranged by size:
1121 993 855 685 191 145 110
SfoI GGC'GCC
Cuts at: 1532 1780 1532
Size: 248 3852
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
SmaI CCC'GGG
Cuts at: 1122 1122
Size: 4100
SmlI C'TyrA_G
Cuts at: 1255 2170 2662 2924 1255
Size: 915 492 262 2431
Fragments arranged by size:
2431 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4100
SphI G_CATG'C
Cuts at: 1153 1653 1153
Size: 500 3600
StuI AGG'CCT
Cuts at: 186 186
Size: 4100
StyI C'CwwG_G
Cuts at: 181 1234 1588 181
Size: 1053 354 2693
Fragments arranged by size:
2693 1053 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1683 1816 2275 2484 1683
Size: 133 459 209 3299
Fragments arranged by size:
3299 459 209 133
TatI w'GTAC_w
Cuts at: 803 3615 803
Size: 2812 1288
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 727 759 3617 3649 727
Size: 32 2858 32 1178
Fragments arranged by size:
2858 1178 32 32
Tth111I GACn'n_nGTC
Cuts at: 1897 1897
Size: 4100
XbaI T'CTAG_A
Cuts at: 1131 1131
Size: 4100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 331 729 1595 114
Size: 217 398 866 2619
Fragments arranged by size:
2619 866 398 217
XmaI C'CCGG_G
Cuts at: 1120 1120
Size: 4100
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI