Restriction map for
pF1KB6480
(Circular) (Six-base) MAPSORT of: pF1KB6480.seq Check: 5614 from: 1 to: 4124
pF1KB6480 4124 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AatII G_ACGT'C
Cuts at: 1055 1055
Size: 4124
AccI GT'mk_AC
Cuts at: 293 1162 293
Size: 869 3255
Acc65I G'GTAC_C
Cuts at: 1140 1140
Size: 4124
AclI AA'CG_TT
Cuts at: 3964 3964
Size: 4124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1949 2381 3128 3396 826
Size: 1123 432 747 268 1554
Fragments arranged by size:
1554 1123 747 432 268
AflIII A'CryG_T
Cuts at: 1476 2580 3557 1476
Size: 1104 977 2043
Fragments arranged by size:
2043 1104 977
AgeI A'CCGG_T
Cuts at: 2512 2512
Size: 4124
AhdI GACnn_n'nnGTC
Cuts at: 345 1065 345
Size: 720 3404
AlwNI CAG_nnn'CTG
Cuts at: 246 1429 2996 246
Size: 1183 1567 1374
Fragments arranged by size:
1567 1374 1183
ApaLI G'TGCA_C
Cuts at: 2894 2894
Size: 4124
ApoI r'AATT_y
Cuts at: 1128 3477 3733 1128
Size: 2349 256 1519
Fragments arranged by size:
2349 1519 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4124
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
AvaI C'yCGr_G
Cuts at: 1144 1144
Size: 4124
BamHI G'GATC_C
Cuts at: 1149 1149
Size: 4124
BanI G'GyrC_C
Cuts at: 1140 1554 1802 1837 1140
Size: 414 248 35 3427
Fragments arranged by size:
3427 414 248 35
BanII G_rGCy'C
Cuts at: 1138 2168 1138
Size: 1030 3094
BbeI G_GCGC'C
Cuts at: 1558 1806 1558
Size: 248 3876
BbsI GAAGACnn'nnnn_
Cuts at: 144 144
Size: 4124
BbvCI CC'TCA_GC
Cuts at: 206 411 206
Size: 205 3919
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4090
BciVI GTATCCnnnnn_n'
Cuts at: 2022 2783 4124 2022
Size: 761 1341 2022
Fragments arranged by size:
2022 1341 761
BclI T'GATC_A
Cuts at: 1644 1644
Size: 4124
BglI GCCn_nnn'nGGC
Cuts at: 169 169
Size: 4124
BglII A'GATC_T
Cuts at: 1639 3676 1639
Size: 2037 2087
BlpI GC'TnA_GC
Cuts at: 458 1236 458
Size: 778 3346
Bme1580I G_kGCm'C
Cuts at: 1749 1842 2898 1749
Size: 93 1056 2975
Fragments arranged by size:
2975 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 654 1336 1417 1752 3896 654
Size: 682 81 335 2144 882
Fragments arranged by size:
2144 882 682 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 4124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 705 518
Size: 187 3937
Bpu10I CC'TnA_GC
Cuts at: 206 268 411 206
Size: 62 143 3919
Fragments arranged by size:
3919 143 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1300 2179 2671 2969 1300
Size: 879 492 298 2455
Fragments arranged by size:
2455 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3828 3828
Size: 4124
BsaAI yAC'GTr
Cuts at: 1477 1477
Size: 4124
BsaBI GATnn'nnATC
Cuts at: 6 1638 3675 6
Size: 1632 2037 455
Fragments arranged by size:
2037 1632 455
BsaHI Gr'CG_yC
Cuts at: 1052 1555 1803 2505 4001 1052
Size: 503 248 702 1496 1175
Fragments arranged by size:
1496 1175 702 503 248
BsaWI w'CCGG_w
Cuts at: 1083 1313 1834 2512 2786 2933 3344 1083
Size: 230 521 678 274 147 411 1863
Fragments arranged by size:
1863 678 521 411 274 230 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 194 576 194
Size: 382 3742
BseYI C'CCAG_C
Cuts at: 191 462 1549 2307 2884 3380 191
Size: 271 1087 758 577 496 935
Fragments arranged by size:
1087 935 758 577 496 271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 322
Size: 4124
BsiEI CG_ry'CG
Cuts at: 104 115 338 1666 1712 2920 104
Size: 11 223 1328 46 1208 1308
Fragments arranged by size:
1328 1308 1208 223 46 11
BsiHKAI G_wGCw'C
Cuts at: 471 1138 1916 2106 2898 471
Size: 667 778 190 792 1697
Fragments arranged by size:
1697 792 778 667 190
Bsp1286I G_dGCh'C
Cuts at: 471 1138 1749 1842 1916 2106 2168 2898 471
Size: 667 611 93 74 190 62 730 1697
Fragments arranged by size:
1697 730 667 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1083 3344 1083
Size: 2261 1863
BspHI T'CATG_A
Cuts at: 3300 3300
Size: 4124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 231 1174 1388 1690 2071 231
Size: 943 214 302 381 2284
Fragments arranged by size:
2284 943 381 302 214
BsrBI CCG'CTC
Cuts at: 50 202 2416 2470 3954 4119 50
Size: 152 2214 54 1484 165 55
Fragments arranged by size:
2214 1484 165 152 55 54
BsrDI GCAATG_nn'
Cuts at: 2036 2036
Size: 4124
BsrFI r'CCGG_y
Cuts at: 2122 2303 2512 2122
Size: 181 209 3734
Fragments arranged by size:
3734 209 181
BsrGI T'GTAC_A
Cuts at: 227 364 227
Size: 137 3987
BssSI C'ACGA_G
Cuts at: 279 2395 2753 279
Size: 2116 358 1650
Fragments arranged by size:
2116 1650 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1501 1501
Size: 4124
BstBI TT'CG_AA
Cuts at: 2485 2485
Size: 4124
BstEII G'GTnAC_C
Cuts at: 549 549
Size: 4124
BstXI CCAn_nnnn'nTGG
Cuts at: 702 702
Size: 4124
BstYI r'GATC_y
Cuts at: 1 1149 1616 1639 1974 2220 3232 3318 3330
Size: 1148 467 23 335 246 1012 86 12
Cuts at: 3330 3676 1
Size: 346 449
Fragments arranged by size:
1148 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 167 614 863 167
Size: 447 249 3428
Fragments arranged by size:
3428 447 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 585 2074 2255 2418 585
Size: 1489 181 163 2291
Fragments arranged by size:
2291 1489 181 163
BtsI GCAGTG_nn'
Cuts at: 395 1367 395
Size: 972 3152
DraI TTT'AAA
Cuts at: 309 992 1123 309
Size: 683 131 3310
Fragments arranged by size:
3310 683 131
DrdI GACnn_nn'nnGTC
Cuts at: 1830 2688 1830
Size: 858 3266
EaeI y'GGCC_r
Cuts at: 335 1709 1883 2274 2301 3646 335
Size: 1374 174 391 27 1345 813
Fragments arranged by size:
1374 1345 813 391 174 27
EagI C'GGCC_G
Cuts at: 335 1709 335
Size: 1374 2750
EarI CTCTTCn'nnn_
Cuts at: 977 2147 2357 977
Size: 1170 210 2744
Fragments arranged by size:
2744 1170 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2332 2642 2788 3934 2332
Size: 310 146 1146 2522
Fragments arranged by size:
2522 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1136 1136
Size: 4124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 518 705 826 1949 2381 3128 3396 518
Size: 187 121 1123 432 747 268 1246
Fragments arranged by size:
1246 1123 747 432 268 187 121
EcoO109I rG'GnC_Cy
Cuts at: 243 676 1263 243
Size: 433 587 3104
Fragments arranged by size:
3104 587 433
EcoRI G'AATT_C
Cuts at: 1128 1128
Size: 4124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 4124
FspI TGC'GCA
Cuts at: 1905 1905
Size: 4124
HaeII r_GCGC'y
Cuts at: 1558 1806 2828 3807 1558
Size: 248 1022 979 1875
Fragments arranged by size:
1875 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 331 363 1051 1083 1966 1996 1998 2028 331
Size: 32 688 32 883 30 2 30 2427
Fragments arranged by size:
2427 883 688 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1163 1163
Size: 4124
KasI G'GCGC_C
Cuts at: 1554 1802 1554
Size: 248 3876
KpnI G_GTAC'C
Cuts at: 1144 1144
Size: 4124
MluI A'CGCG_T
Cuts at: 3557 3557
Size: 4124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 687 2795 2979 687
Size: 2108 184 1832
Fragments arranged by size:
2108 1832 184
MscI TGG'CCA
Cuts at: 1885 1885
Size: 4124
MslI CAynn'nnrTG
Cuts at: 355 547 2113 2240 355
Size: 192 1566 127 2239
Fragments arranged by size:
2239 1566 192 127
MspA1I CmG'CkG
Cuts at: 169 195 1235 1549 1909 2922 3167 169
Size: 26 1040 314 360 1013 245 1126
Fragments arranged by size:
1126 1040 1013 360 314 245 26
NaeI GCC'GGC
Cuts at: 2305 2305
Size: 4124
NarI GG'CG_CC
Cuts at: 1555 1803 1555
Size: 248 3876
NgoMIV G'CCGG_C
Cuts at: 2303 2303
Size: 4124
NspI r_CATG'y
Cuts at: 1177 1677 2584 1177
Size: 500 907 2717
Fragments arranged by size:
2717 907 500
PciI A'CATG_T
Cuts at: 2580 2580
Size: 4124
PmeI GTTT'AAAC
Cuts at: 1123 1123
Size: 4124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 4092
PpuMI rG'GwC_Cy
Cuts at: 243 243
Size: 4124
PstI C_TGCA'G
Cuts at: 1171 1856 1171
Size: 685 3439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4124
PvuII CAG'CTG
Cuts at: 195 1549 1909 195
Size: 1354 360 2410
Fragments arranged by size:
2410 1354 360
RsrII CG'GwC_CG
Cuts at: 2319 2319
Size: 4124
SacI G_AGCT'C
Cuts at: 1138 1138
Size: 4124
SacII CC_GC'GG
Cuts at: 170 170
Size: 4124
SalI G'TCGA_C
Cuts at: 1161 1161
Size: 4124
SapI GCTCTTCn'nnn_
Cuts at: 2147 2357 2147
Size: 210 3914
SbfI CC_TGCA'GG
Cuts at: 1171 1171
Size: 4124
ScaI AGT'ACT
Cuts at: 3641 3641
Size: 4124
SfcI C'TryA_G
Cuts at: 33 1167 1852 2845 3036 33
Size: 1134 685 993 191 1121
Fragments arranged by size:
1134 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1556 1804 1556
Size: 248 3876
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
SmaI CCC'GGG
Cuts at: 1146 1146
Size: 4124
SmlI C'TyrA_G
Cuts at: 1279 2194 2686 2948 1279
Size: 915 492 262 2455
Fragments arranged by size:
2455 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4124
SphI G_CATG'C
Cuts at: 1177 1677 1177
Size: 500 3624
StuI AGG'CCT
Cuts at: 285 932 1021 285
Size: 647 89 3388
Fragments arranged by size:
3388 647 89
StyI C'CwwG_G
Cuts at: 496 1022 1258 1612 496
Size: 526 236 354 3008
Fragments arranged by size:
3008 526 354 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 129 474 1707 1840 2299 2508 129
Size: 345 1233 133 459 209 1745
Fragments arranged by size:
1745 1233 459 345 209 133
TatI w'GTAC_w
Cuts at: 227 364 728 3639 227
Size: 137 364 2911 712
Fragments arranged by size:
2911 712 364 137
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 950 982 3641 3673 950
Size: 32 2659 32 1401
Fragments arranged by size:
2659 1401 32 32
Tth111I GACn'n_nGTC
Cuts at: 1921 1921
Size: 4124
XbaI T'CTAG_A
Cuts at: 1155 1155
Size: 4124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1619 1619
Size: 4124
XmaI C'CCGG_G
Cuts at: 1144 1144
Size: 4124
XmnI GAAnn'nnTTC
Cuts at: 873 1103 873
Size: 230 3894
ZraI GAC'GTC
Cuts at: 1053 1053
Size: 4124
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI