Restriction map for
pF1KB6477
(Circular) (Six-base) MAPSORT of: pF1KB6477.seq Check: 846 from: 1 to: 4091
pF1KB6477 4091 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 889 1129 889
Size: 240 3851
Acc65I G'GTAC_C
Cuts at: 1107 1107
Size: 4091
AclI AA'CG_TT
Cuts at: 3931 3931
Size: 4091
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1916 2348 3095 3363 502
Size: 1414 432 747 268 1230
Fragments arranged by size:
1414 1230 747 432 268
AflIII A'CryG_T
Cuts at: 1443 2547 3524 1443
Size: 1104 977 2010
Fragments arranged by size:
2010 1104 977
AgeI A'CCGG_T
Cuts at: 2479 2479
Size: 4091
AhdI GACnn_n'nnGTC
Cuts at: 226 226
Size: 4091
AlwNI CAG_nnn'CTG
Cuts at: 183 480 666 1396 2963 183
Size: 297 186 730 1567 1311
Fragments arranged by size:
1567 1311 730 297 186
ApaLI G'TGCA_C
Cuts at: 2861 2861
Size: 4091
ApoI r'AATT_y
Cuts at: 334 346 563 1095 3444 3700 334
Size: 12 217 532 2349 256 725
Fragments arranged by size:
2349 725 532 256 217 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 4091
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
AvaI C'yCGr_G
Cuts at: 1111 1111
Size: 4091
BamHI G'GATC_C
Cuts at: 612 1116 612
Size: 504 3587
BanI G'GyrC_C
Cuts at: 239 833 1107 1521 1769 1804 239
Size: 594 274 414 248 35 2526
Fragments arranged by size:
2526 594 414 274 248 35
BanII G_rGCy'C
Cuts at: 251 511 1105 2135 251
Size: 260 594 1030 2207
Fragments arranged by size:
2207 1030 594 260
BbeI G_GCGC'C
Cuts at: 837 1525 1773 837
Size: 688 248 3155
Fragments arranged by size:
3155 688 248
BciVI GTATCCnnnnn_n'
Cuts at: 1989 2750 4091 1989
Size: 761 1341 1989
Fragments arranged by size:
1989 1341 761
BclI T'GATC_A
Cuts at: 1611 1611
Size: 4091
BglI GCCn_nnn'nGGC
Cuts at: 391 391
Size: 4091
BglII A'GATC_T
Cuts at: 1606 3643 1606
Size: 2037 2054
BlpI GC'TnA_GC
Cuts at: 154 1044 1203 154
Size: 890 159 3042
Fragments arranged by size:
3042 890 159
Bme1580I G_kGCm'C
Cuts at: 1716 1809 2865 1716
Size: 93 1056 2942
Fragments arranged by size:
2942 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1303 1384 1719 3863 1303
Size: 81 335 2144 1531
Fragments arranged by size:
2144 1531 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 179 2458 179
Size: 2279 1812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 871 154
Size: 717 3374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 2146 2638 2936 1267
Size: 879 492 298 2422
Fragments arranged by size:
2422 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3795 3795
Size: 4091
BsaAI yAC'GTr
Cuts at: 1444 1444
Size: 4091
BsaBI GATnn'nnATC
Cuts at: 6 1605 3642 6
Size: 1599 2037 455
Fragments arranged by size:
2037 1599 455
BsaHI Gr'CG_yC
Cuts at: 834 1522 1770 2472 3968 834
Size: 688 248 702 1496 957
Fragments arranged by size:
1496 957 702 688 248
BsaWI w'CCGG_w
Cuts at: 1280 1801 2479 2753 2900 3311 1280
Size: 521 678 274 147 411 2060
Fragments arranged by size:
2060 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 586 589 596 586
Size: 3 7 4081
Fragments arranged by size:
4081 7 3
BseYI C'CCAG_C
Cuts at: 1063 1516 2274 2851 3347 1063
Size: 453 758 577 496 1807
Fragments arranged by size:
1807 758 577 496 453
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 196
Size: 4091
BsiEI CG_ry'CG
Cuts at: 104 1633 1679 2887 104
Size: 1529 46 1208 1308
Fragments arranged by size:
1529 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 251 1105 1883 2073 2865 251
Size: 854 778 190 792 1477
Fragments arranged by size:
1477 854 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 234 234
Size: 4091
Bsp1286I G_dGCh'C
Cuts at: 251 511 1105 1716 1809 1883 2073 2135 2865
Size: 260 594 611 93 74 190 62 730
Cuts at: 2865 251
Size: 1477
Fragments arranged by size:
1477 730 611 594 260 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3311 3311
Size: 4091
BspHI T'CATG_A
Cuts at: 3267 3267
Size: 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1141 1355 1657 2038 1141
Size: 214 302 381 3194
Fragments arranged by size:
3194 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2383 2437 3921 4086 50
Size: 2333 54 1484 165 55
Fragments arranged by size:
2333 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2003 2003
Size: 4091
BsrFI r'CCGG_y
Cuts at: 2089 2270 2479 2089
Size: 181 209 3701
Fragments arranged by size:
3701 209 181
BssSI C'ACGA_G
Cuts at: 959 2362 2720 959
Size: 1403 358 2330
Fragments arranged by size:
2330 1403 358
BstAPI GCAn_nnn'nTGC
Cuts at: 174 1468 174
Size: 1294 2797
BstBI TT'CG_AA
Cuts at: 2452 2452
Size: 4091
BstYI r'GATC_y
Cuts at: 1 612 663 1116 1583 1606 1941 2187 3199
Size: 611 51 453 467 23 335 246 1012
Cuts at: 3199 3285 3297 3643 1
Size: 86 12 346 449
Fragments arranged by size:
1012 611 467 453 449 346 335 246
86 51 23 12
BstZ17I GTA'TAC
Cuts at: 890 890
Size: 4091
Bsu36I CC'TnA_GG
Cuts at: 608 608
Size: 4091
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2041 2222 2385 2041
Size: 181 163 3747
Fragments arranged by size:
3747 181 163
BtsI GCAGTG_nn'
Cuts at: 1018 1334 1018
Size: 316 3775
DraI TTT'AAA
Cuts at: 1090 1090
Size: 4091
DrdI GACnn_nn'nnGTC
Cuts at: 1797 2655 1797
Size: 858 3233
EaeI y'GGCC_r
Cuts at: 1676 1850 2241 2268 3613 1676
Size: 174 391 27 1345 2154
Fragments arranged by size:
2154 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1676 1676
Size: 4091
EarI CTCTTCn'nnn_
Cuts at: 524 2114 2324 524
Size: 1590 210 2291
Fragments arranged by size:
2291 1590 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2299 2609 2755 3901 2299
Size: 310 146 1146 2489
Fragments arranged by size:
2489 1146 310 146
EcoICRI GAG'CTC
Cuts at: 249 1103 249
Size: 854 3237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 502 871 1916 2348 3095 3363 154
Size: 348 369 1045 432 747 268 882
Fragments arranged by size:
1045 882 747 432 369 348 268
EcoNI CCTnn'n_nnAGG
Cuts at: 620 620
Size: 4091
EcoO109I rG'GnC_Cy
Cuts at: 220 328 625 1068 1230 220
Size: 108 297 443 162 3081
Fragments arranged by size:
3081 443 297 162 108
EcoRI G'AATT_C
Cuts at: 334 1095 334
Size: 761 3330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 4091
FspI TGC'GCA
Cuts at: 1872 1872
Size: 4091
HaeII r_GCGC'y
Cuts at: 133 837 1525 1773 2795 3774 133
Size: 704 688 248 1022 979 450
Fragments arranged by size:
1022 979 704 688 450 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 212 244 523 555 898 930 1933 1963 1965
Size: 32 279 32 343 32 1003 30 2
Cuts at: 1965 1995 212
Size: 30 2308
Fragments arranged by size:
2308 1003 343 279 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1130 1130
Size: 4091
HindIII A'AGCT_T
Cuts at: 274 653 952 274
Size: 379 299 3413
Fragments arranged by size:
3413 379 299
KasI G'GCGC_C
Cuts at: 833 1521 1769 833
Size: 688 248 3155
Fragments arranged by size:
3155 688 248
KpnI G_GTAC'C
Cuts at: 1111 1111
Size: 4091
MluI A'CGCG_T
Cuts at: 3524 3524
Size: 4091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 723 896 2762 2946 723
Size: 173 1866 184 1868
Fragments arranged by size:
1868 1866 184 173
MscI TGG'CCA
Cuts at: 1852 1852
Size: 4091
MslI CAynn'nnrTG
Cuts at: 2080 2207 2080
Size: 127 3964
MspA1I CmG'CkG
Cuts at: 480 775 867 1202 1516 1876 2889 3134 480
Size: 295 92 335 314 360 1013 245 1437
Fragments arranged by size:
1437 1013 360 335 314 295 245 92
NaeI GCC'GGC
Cuts at: 2272 2272
Size: 4091
NarI GG'CG_CC
Cuts at: 834 1522 1770 834
Size: 688 248 3155
Fragments arranged by size:
3155 688 248
NgoMIV G'CCGG_C
Cuts at: 2270 2270
Size: 4091
NspI r_CATG'y
Cuts at: 809 1144 1644 2551 809
Size: 335 500 907 2349
Fragments arranged by size:
2349 907 500 335
PciI A'CATG_T
Cuts at: 2547 2547
Size: 4091
PmeI GTTT'AAAC
Cuts at: 1090 1090
Size: 4091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 4059
PpuMI rG'GwC_Cy
Cuts at: 220 328 625 1068 220
Size: 108 297 443 3243
Fragments arranged by size:
3243 443 297 108
PstI C_TGCA'G
Cuts at: 624 1138 1823 624
Size: 514 685 2892
Fragments arranged by size:
2892 685 514
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4091
PvuII CAG'CTG
Cuts at: 480 775 867 1516 1876 480
Size: 295 92 649 360 2695
Fragments arranged by size:
2695 649 360 295 92
RsrII CG'GwC_CG
Cuts at: 2286 2286
Size: 4091
SacI G_AGCT'C
Cuts at: 251 1105 251
Size: 854 3237
SalI G'TCGA_C
Cuts at: 1128 1128
Size: 4091
SanDI GG'GwC_CC
Cuts at: 220 220
Size: 4091
SapI GCTCTTCn'nnn_
Cuts at: 2114 2324 2114
Size: 210 3881
SbfI CC_TGCA'GG
Cuts at: 1138 1138
Size: 4091
ScaI AGT'ACT
Cuts at: 3608 3608
Size: 4091
SfcI C'TryA_G
Cuts at: 33 620 1134 1819 2812 3003 33
Size: 587 514 685 993 191 1121
Fragments arranged by size:
1121 993 685 587 514 191
SfoI GGC'GCC
Cuts at: 835 1523 1771 835
Size: 688 248 3155
Fragments arranged by size:
3155 688 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
SmaI CCC'GGG
Cuts at: 1113 1113
Size: 4091
SmlI C'TyrA_G
Cuts at: 1246 2161 2653 2915 1246
Size: 915 492 262 2422
Fragments arranged by size:
2422 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4091
SphI G_CATG'C
Cuts at: 1144 1644 1144
Size: 500 3591
StyI C'CwwG_G
Cuts at: 1225 1579 1225
Size: 354 3737
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1674 1807 2266 2475 1674
Size: 133 459 209 3290
Fragments arranged by size:
3290 459 209 133
TatI w'GTAC_w
Cuts at: 3606 3606
Size: 4091
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3608 3640 3608
Size: 32 4059
Tth111I GACn'n_nGTC
Cuts at: 183 1888 183
Size: 1705 2386
XbaI T'CTAG_A
Cuts at: 1122 1122
Size: 4091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1586 1586
Size: 4091
XmaI C'CCGG_G
Cuts at: 1111 1111
Size: 4091
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BstZ17I Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII
BstXI BtgI ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI