Restriction map for
pF1KB6473
(Circular) (Six-base) MAPSORT of: pF1KB6473.seq Check: 8057 from: 1 to: 4088
pF1KB6473 4088 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1126 1126
Size: 4088
Acc65I G'GTAC_C
Cuts at: 1104 1104
Size: 4088
AclI AA'CG_TT
Cuts at: 3928 3928
Size: 4088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1913 2345 3092 3360 1060
Size: 853 432 747 268 1788
Fragments arranged by size:
1788 853 747 432 268
AfeI AGC'GCT
Cuts at: 142 142
Size: 4088
AflII C'TTAA_G
Cuts at: 1051 1051
Size: 4088
AflIII A'CryG_T
Cuts at: 1440 2544 3521 1440
Size: 1104 977 2007
Fragments arranged by size:
2007 1104 977
AgeI A'CCGG_T
Cuts at: 2476 2476
Size: 4088
AlwNI CAG_nnn'CTG
Cuts at: 546 1393 2960 546
Size: 847 1567 1674
Fragments arranged by size:
1674 1567 847
ApaLI G'TGCA_C
Cuts at: 2858 2858
Size: 4088
ApoI r'AATT_y
Cuts at: 334 1024 1092 3441 3697 334
Size: 690 68 2349 256 725
Fragments arranged by size:
2349 725 690 256 68
AseI AT'TA_AT
Cuts at: 20 20
Size: 4088
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
AvaI C'yCGr_G
Cuts at: 1108 1108
Size: 4088
BamHI G'GATC_C
Cuts at: 1113 1113
Size: 4088
BanI G'GyrC_C
Cuts at: 1104 1518 1766 1801 1104
Size: 414 248 35 3391
Fragments arranged by size:
3391 414 248 35
BanII G_rGCy'C
Cuts at: 1102 2132 1102
Size: 1030 3058
BbeI G_GCGC'C
Cuts at: 1522 1770 1522
Size: 248 3840
BciVI GTATCCnnnnn_n'
Cuts at: 1986 2747 4088 1986
Size: 761 1341 1986
Fragments arranged by size:
1986 1341 761
BclI T'GATC_A
Cuts at: 1608 1608
Size: 4088
BglII A'GATC_T
Cuts at: 1603 3640 1603
Size: 2037 2051
BlpI GC'TnA_GC
Cuts at: 1200 1200
Size: 4088
Bme1580I G_kGCm'C
Cuts at: 1713 1806 2862 1713
Size: 93 1056 2939
Fragments arranged by size:
2939 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1300 1381 1716 3860 1300
Size: 81 335 2144 1528
Fragments arranged by size:
2144 1528 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 4088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1264 2143 2635 2933 952
Size: 312 879 492 298 2107
Fragments arranged by size:
2107 879 492 312 298
BsaI GGTCTCn'nnnn_
Cuts at: 903 1060 3792 903
Size: 157 2732 1199
Fragments arranged by size:
2732 1199 157
BsaAI yAC'GTr
Cuts at: 1441 1441
Size: 4088
BsaBI GATnn'nnATC
Cuts at: 6 1602 3639 6
Size: 1596 2037 455
Fragments arranged by size:
2037 1596 455
BsaHI Gr'CG_yC
Cuts at: 1519 1767 2469 3965 1519
Size: 248 702 1496 1642
Fragments arranged by size:
1642 1496 702 248
BsaWI w'CCGG_w
Cuts at: 381 1277 1798 2476 2750 2897 3308 381
Size: 896 521 678 274 147 411 1161
Fragments arranged by size:
1161 896 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 445 1513 2271 2848 3344 445
Size: 1068 758 577 496 1189
Fragments arranged by size:
1189 1068 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1630 1676 2884 104
Size: 1526 46 1208 1308
Fragments arranged by size:
1526 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1102 1880 2070 2862 1102
Size: 778 190 792 2328
Fragments arranged by size:
2328 792 778 190
BsmI GAATG_Cn'
Cuts at: 447 447
Size: 4088
Bsp1286I G_dGCh'C
Cuts at: 1102 1713 1806 1880 2070 2132 2862 1102
Size: 611 93 74 190 62 730 2328
Fragments arranged by size:
2328 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 381 3308 381
Size: 2927 1161
BspHI T'CATG_A
Cuts at: 3264 3264
Size: 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 955 1138 1352 1654 2035 955
Size: 183 214 302 381 3008
Fragments arranged by size:
3008 381 302 214 183
BsrBI CCG'CTC
Cuts at: 50 2380 2434 3918 4083 50
Size: 2330 54 1484 165 55
Fragments arranged by size:
2330 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2000 2000
Size: 4088
BsrFI r'CCGG_y
Cuts at: 2086 2267 2476 2086
Size: 181 209 3698
Fragments arranged by size:
3698 209 181
BssSI C'ACGA_G
Cuts at: 2359 2717 2359
Size: 358 3730
BstAPI GCAn_nnn'nTGC
Cuts at: 1465 1465
Size: 4088
BstBI TT'CG_AA
Cuts at: 2449 2449
Size: 4088
BstXI CCAn_nnnn'nTGG
Cuts at: 120 850 120
Size: 730 3358
BstYI r'GATC_y
Cuts at: 1 1113 1580 1603 1938 2184 3196 3282 3294
Size: 1112 467 23 335 246 1012 86 12
Cuts at: 3294 3640 1
Size: 346 449
Fragments arranged by size:
1112 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1071 1071
Size: 4088
BtgI C'CryG_G
Cuts at: 107 107
Size: 4088
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 778 2038 2219 2382 778
Size: 1260 181 163 2484
Fragments arranged by size:
2484 1260 181 163
BtsI GCAGTG_nn'
Cuts at: 214 745 867 1331 214
Size: 531 122 464 2971
Fragments arranged by size:
2971 531 464 122
ClaI AT'CG_AT
Cuts at: 533 533
Size: 4088
DraI TTT'AAA
Cuts at: 1087 1087
Size: 4088
DrdI GACnn_nn'nnGTC
Cuts at: 458 1794 2652 458
Size: 1336 858 1894
Fragments arranged by size:
1894 1336 858
EaeI y'GGCC_r
Cuts at: 110 122 345 1673 1847 2238 2265 3610 110
Size: 12 223 1328 174 391 27 1345 588
Fragments arranged by size:
1345 1328 588 391 223 174 27 12
EagI C'GGCC_G
Cuts at: 1673 1673
Size: 4088
EarI CTCTTCn'nnn_
Cuts at: 259 2111 2321 259
Size: 1852 210 2026
Fragments arranged by size:
2026 1852 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2296 2606 2752 3898 2296
Size: 310 146 1146 2486
Fragments arranged by size:
2486 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1100 1100
Size: 4088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1913 2345 3092 3360 1060
Size: 853 432 747 268 1788
Fragments arranged by size:
1788 853 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 406 1074 406
Size: 668 3420
EcoO109I rG'GnC_Cy
Cuts at: 675 1227 675
Size: 552 3536
EcoRI G'AATT_C
Cuts at: 334 1092 334
Size: 758 3330
EcoRV GAT'ATC
Cuts at: 426 426
Size: 4088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 171 2107 171
Size: 1936 2152
FspI TGC'GCA
Cuts at: 1869 1869
Size: 4088
HaeII r_GCGC'y
Cuts at: 131 144 1522 1770 2792 3771 131
Size: 13 1378 248 1022 979 448
Fragments arranged by size:
1378 1022 979 448 248 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 689 721 883 915 1930 1960 1962 1992 689
Size: 32 162 32 1015 30 2 30 2785
Fragments arranged by size:
2785 1015 162 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1127 1127
Size: 4088
KasI G'GCGC_C
Cuts at: 1518 1766 1518
Size: 248 3840
KpnI G_GTAC'C
Cuts at: 1108 1108
Size: 4088
MluI A'CGCG_T
Cuts at: 3521 3521
Size: 4088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2759 2943 2759
Size: 184 3904
MscI TGG'CCA
Cuts at: 112 124 1849 112
Size: 12 1725 2351
Fragments arranged by size:
2351 1725 12
MslI CAynn'nnrTG
Cuts at: 326 476 2077 2204 326
Size: 150 1601 127 2210
Fragments arranged by size:
2210 1601 150 127
MspA1I CmG'CkG
Cuts at: 166 374 395 1199 1513 1873 2886 3131 166
Size: 208 21 804 314 360 1013 245 1123
Fragments arranged by size:
1123 1013 804 360 314 245 208 21
NaeI GCC'GGC
Cuts at: 2269 2269
Size: 4088
NarI GG'CG_CC
Cuts at: 1519 1767 1519
Size: 248 3840
NcoI C'CATG_G
Cuts at: 107 107
Size: 4088
NgoMIV G'CCGG_C
Cuts at: 2267 2267
Size: 4088
NspI r_CATG'y
Cuts at: 748 1141 1641 2548 748
Size: 393 500 907 2288
Fragments arranged by size:
2288 907 500 393
PciI A'CATG_T
Cuts at: 2544 2544
Size: 4088
PmeI GTTT'AAAC
Cuts at: 1087 1087
Size: 4088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 4056
PpuMI rG'GwC_Cy
Cuts at: 675 675
Size: 4088
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 550 582 550
Size: 32 4056
PstI C_TGCA'G
Cuts at: 1135 1820 1135
Size: 685 3403
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4088
PvuII CAG'CTG
Cuts at: 374 1513 1873 374
Size: 1139 360 2589
Fragments arranged by size:
2589 1139 360
RsrII CG'GwC_CG
Cuts at: 2283 2283
Size: 4088
SacI G_AGCT'C
Cuts at: 1102 1102
Size: 4088
SalI G'TCGA_C
Cuts at: 1125 1125
Size: 4088
SanDI GG'GwC_CC
Cuts at: 675 675
Size: 4088
SapI GCTCTTCn'nnn_
Cuts at: 2111 2321 2111
Size: 210 3878
SbfI CC_TGCA'GG
Cuts at: 1135 1135
Size: 4088
ScaI AGT'ACT
Cuts at: 280 3605 280
Size: 3325 763
SfcI C'TryA_G
Cuts at: 33 1131 1816 2809 3000 33
Size: 1098 685 993 191 1121
Fragments arranged by size:
1121 1098 993 685 191
SfoI GGC'GCC
Cuts at: 1520 1768 1520
Size: 248 3840
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
SmaI CCC'GGG
Cuts at: 1110 1110
Size: 4088
SmlI C'TyrA_G
Cuts at: 967 1051 1243 2158 2650 2912 967
Size: 84 192 915 492 262 2143
Fragments arranged by size:
2143 915 492 262 192 84
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4088
SphI G_CATG'C
Cuts at: 1141 1641 1141
Size: 500 3588
StuI AGG'CCT
Cuts at: 494 815 916 494
Size: 321 101 3666
Fragments arranged by size:
3666 321 101
StyI C'CwwG_G
Cuts at: 107 495 1222 1576 107
Size: 388 727 354 2619
Fragments arranged by size:
2619 727 388 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1671 1804 2263 2472 1671
Size: 133 459 209 3287
Fragments arranged by size:
3287 459 209 133
TatI w'GTAC_w
Cuts at: 278 3603 278
Size: 3325 763
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3605 3637 3605
Size: 32 4056
Tth111I GACn'n_nGTC
Cuts at: 1885 1885
Size: 4088
XbaI T'CTAG_A
Cuts at: 1119 1119
Size: 4088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1583 1583
Size: 4088
XmaI C'CCGG_G
Cuts at: 1108 1108
Size: 4088
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI