Restriction map for
pF1KB6469
(Circular) (Six-base) MAPSORT of: pF1KB6469.seq Check: 2916 from: 1 to: 4085
pF1KB6469 4085 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AatII G_ACGT'C
Cuts at: 806 806
Size: 4085
AccI GT'mk_AC
Cuts at: 1123 1123
Size: 4085
Acc65I G'GTAC_C
Cuts at: 389 1101 389
Size: 712 3373
AclI AA'CG_TT
Cuts at: 3925 3925
Size: 4085
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1910 2342 3089 3357 880
Size: 1030 432 747 268 1608
Fragments arranged by size:
1608 1030 747 432 268
AflIII A'CryG_T
Cuts at: 338 576 1437 2541 3518 338
Size: 238 861 1104 977 905
Fragments arranged by size:
1104 977 905 861 238
AgeI A'CCGG_T
Cuts at: 2473 2473
Size: 4085
AleI CACnn'nnGTG
Cuts at: 655 655
Size: 4085
AlwNI CAG_nnn'CTG
Cuts at: 1390 2957 1390
Size: 1567 2518
ApaLI G'TGCA_C
Cuts at: 2855 2855
Size: 4085
ApoI r'AATT_y
Cuts at: 1089 3438 3694 1089
Size: 2349 256 1480
Fragments arranged by size:
2349 1480 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4085
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
AvaI C'yCGr_G
Cuts at: 1105 1105
Size: 4085
AvrII C'CTAG_G
Cuts at: 498 498
Size: 4085
BamHI G'GATC_C
Cuts at: 1110 1110
Size: 4085
BanI G'GyrC_C
Cuts at: 389 1101 1515 1763 1798 389
Size: 712 414 248 35 2676
Fragments arranged by size:
2676 712 414 248 35
BanII G_rGCy'C
Cuts at: 1099 2129 1099
Size: 1030 3055
BbeI G_GCGC'C
Cuts at: 1519 1767 1519
Size: 248 3837
BbsI GAAGACnn'nnnn_
Cuts at: 798 868 798
Size: 70 4015
BbvCI CC'TCA_GC
Cuts at: 608 608
Size: 4085
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4051
BciVI GTATCCnnnnn_n'
Cuts at: 1983 2744 4085 1983
Size: 761 1341 1983
Fragments arranged by size:
1983 1341 761
BclI T'GATC_A
Cuts at: 1605 1605
Size: 4085
BglII A'GATC_T
Cuts at: 1600 3637 1600
Size: 2037 2048
BlpI GC'TnA_GC
Cuts at: 1197 1197
Size: 4085
Bme1580I G_kGCm'C
Cuts at: 664 1710 1803 2859 664
Size: 1046 93 1056 1890
Fragments arranged by size:
1890 1056 1046 93
BmrI ACTGGGnnnn_n'
Cuts at: 1297 1378 1713 3857 1297
Size: 81 335 2144 1525
Fragments arranged by size:
2144 1525 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4085
Bpu10I CC'TnA_GC
Cuts at: 608 608
Size: 4085
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 2140 2632 2930 1261
Size: 879 492 298 2416
Fragments arranged by size:
2416 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3789 3789
Size: 4085
BsaAI yAC'GTr
Cuts at: 579 1438 579
Size: 859 3226
BsaBI GATnn'nnATC
Cuts at: 6 1599 3636 6
Size: 1593 2037 455
Fragments arranged by size:
2037 1593 455
BsaHI Gr'CG_yC
Cuts at: 803 1516 1764 2466 3962 803
Size: 713 248 702 1496 926
Fragments arranged by size:
1496 926 713 702 248
BsaWI w'CCGG_w
Cuts at: 1274 1795 2473 2747 2894 3305 1274
Size: 521 678 274 147 411 2054
Fragments arranged by size:
2054 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1075 1075
Size: 4085
BseYI C'CCAG_C
Cuts at: 671 1510 2268 2845 3341 671
Size: 839 758 577 496 1415
Fragments arranged by size:
1415 839 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 907 145
Size: 762 3323
BsiEI CG_ry'CG
Cuts at: 104 1627 1673 2881 104
Size: 1523 46 1208 1308
Fragments arranged by size:
1523 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1099 1877 2067 2859 1099
Size: 778 190 792 2325
Fragments arranged by size:
2325 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 664 1099 1710 1803 1877 2067 2129 2859 664
Size: 435 611 93 74 190 62 730 1890
Fragments arranged by size:
1890 730 611 435 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3305 3305
Size: 4085
BspHI T'CATG_A
Cuts at: 440 3261 440
Size: 2821 1264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 821 1135 1349 1651 2032 821
Size: 314 214 302 381 2874
Fragments arranged by size:
2874 381 314 302 214
BsrBI CCG'CTC
Cuts at: 50 2377 2431 3915 4080 50
Size: 2327 54 1484 165 55
Fragments arranged by size:
2327 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 422 1997 422
Size: 1575 2510
BsrFI r'CCGG_y
Cuts at: 2083 2264 2473 2083
Size: 181 209 3695
Fragments arranged by size:
3695 209 181
BssSI C'ACGA_G
Cuts at: 2356 2714 2356
Size: 358 3727
BstAPI GCAn_nnn'nTGC
Cuts at: 1462 1462
Size: 4085
BstBI TT'CG_AA
Cuts at: 2446 2446
Size: 4085
BstXI CCAn_nnnn'nTGG
Cuts at: 547 694 723 547
Size: 147 29 3909
Fragments arranged by size:
3909 147 29
BstYI r'GATC_y
Cuts at: 1 1110 1577 1600 1935 2181 3193 3279 3291
Size: 1109 467 23 335 246 1012 86 12
Cuts at: 3291 3637 1
Size: 346 449
Fragments arranged by size:
1109 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 693 911 693
Size: 218 3867
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2035 2216 2379 2035
Size: 181 163 3741
Fragments arranged by size:
3741 181 163
BtsI GCAGTG_nn'
Cuts at: 128 1328 128
Size: 1200 2885
DraI TTT'AAA
Cuts at: 1084 1084
Size: 4085
DrdI GACnn_nn'nnGTC
Cuts at: 1791 2649 1791
Size: 858 3227
EaeI y'GGCC_r
Cuts at: 1670 1844 2235 2262 3607 1670
Size: 174 391 27 1345 2148
Fragments arranged by size:
2148 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1670 1670
Size: 4085
EarI CTCTTCn'nnn_
Cuts at: 983 2108 2318 983
Size: 1125 210 2750
Fragments arranged by size:
2750 1125 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2293 2603 2749 3895 2293
Size: 310 146 1146 2483
Fragments arranged by size:
2483 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1097 1097
Size: 4085
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1910 2342 3089 3357 880
Size: 1030 432 747 268 1608
Fragments arranged by size:
1608 1030 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 400 495 1224 400
Size: 95 729 3261
Fragments arranged by size:
3261 729 95
EcoRI G'AATT_C
Cuts at: 1089 1089
Size: 4085
EcoRV GAT'ATC
Cuts at: 642 729 642
Size: 87 3998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 4085
FspI TGC'GCA
Cuts at: 1866 1866
Size: 4085
HaeII r_GCGC'y
Cuts at: 1519 1767 2789 3768 1519
Size: 248 1022 979 1836
Fragments arranged by size:
1836 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1927 1957 1959 1989 1927
Size: 30 2 30 4023
Fragments arranged by size:
4023 30 30 2
HincII GTy'rAC
Cuts at: 1124 1124
Size: 4085
HindIII A'AGCT_T
Cuts at: 718 718
Size: 4085
KasI G'GCGC_C
Cuts at: 1515 1763 1515
Size: 248 3837
KpnI G_GTAC'C
Cuts at: 393 1105 393
Size: 712 3373
MluI A'CGCG_T
Cuts at: 3518 3518
Size: 4085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 174 580 597 2756 2940 174
Size: 406 17 2159 184 1319
Fragments arranged by size:
2159 1319 406 184 17
MscI TGG'CCA
Cuts at: 1846 1846
Size: 4085
MslI CAynn'nnrTG
Cuts at: 416 481 526 545 655 2074 2201 416
Size: 65 45 19 110 1419 127 2300
Fragments arranged by size:
2300 1419 127 110 65 45 19
MspA1I CmG'CkG
Cuts at: 675 795 1196 1510 1870 2883 3128 675
Size: 120 401 314 360 1013 245 1632
Fragments arranged by size:
1632 1013 401 360 314 245 120
NaeI GCC'GGC
Cuts at: 2266 2266
Size: 4085
NarI GG'CG_CC
Cuts at: 1516 1764 1516
Size: 248 3837
NcoI C'CATG_G
Cuts at: 693 693
Size: 4085
NgoMIV G'CCGG_C
Cuts at: 2264 2264
Size: 4085
NspI r_CATG'y
Cuts at: 342 1138 1638 2545 342
Size: 796 500 907 1882
Fragments arranged by size:
1882 907 796 500
PciI A'CATG_T
Cuts at: 338 2541 338
Size: 2203 1882
PflMI CCAn_nnn'nTGG
Cuts at: 147 879 147
Size: 732 3353
PmeI GTTT'AAAC
Cuts at: 1084 1084
Size: 4085
PmlI CAC'GTG
Cuts at: 579 579
Size: 4085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 4053
PpuMI rG'GwC_Cy
Cuts at: 495 495
Size: 4085
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 617 649 617
Size: 32 4053
PstI C_TGCA'G
Cuts at: 800 836 1132 1817 800
Size: 36 296 685 3068
Fragments arranged by size:
3068 685 296 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4085
PvuII CAG'CTG
Cuts at: 795 1510 1870 795
Size: 715 360 3010
Fragments arranged by size:
3010 715 360
RsrII CG'GwC_CG
Cuts at: 2280 2280
Size: 4085
SacI G_AGCT'C
Cuts at: 1099 1099
Size: 4085
SalI G'TCGA_C
Cuts at: 1122 1122
Size: 4085
SapI GCTCTTCn'nnn_
Cuts at: 2108 2318 2108
Size: 210 3875
SbfI CC_TGCA'GG
Cuts at: 836 1132 836
Size: 296 3789
ScaI AGT'ACT
Cuts at: 248 3602 248
Size: 3354 731
SexAI A'CCwGG_T
Cuts at: 686 686
Size: 4085
SfcI C'TryA_G
Cuts at: 33 509 796 832 1128 1813 2806 2997 33
Size: 476 287 36 296 685 993 191 1121
Fragments arranged by size:
1121 993 685 476 296 287 191 36
SfoI GGC'GCC
Cuts at: 1517 1765 1517
Size: 248 3837
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
SmaI CCC'GGG
Cuts at: 1107 1107
Size: 4085
SmlI C'TyrA_G
Cuts at: 1240 2155 2647 2909 1240
Size: 915 492 262 2416
Fragments arranged by size:
2416 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4085
SphI G_CATG'C
Cuts at: 1138 1638 1138
Size: 500 3585
StuI AGG'CCT
Cuts at: 850 850
Size: 4085
StyI C'CwwG_G
Cuts at: 498 693 1219 1573 498
Size: 195 526 354 3010
Fragments arranged by size:
3010 526 354 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1668 1801 2260 2469 1668
Size: 133 459 209 3284
Fragments arranged by size:
3284 459 209 133
TatI w'GTAC_w
Cuts at: 246 887 3600 246
Size: 641 2713 731
Fragments arranged by size:
2713 731 641
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3602 3634 3602
Size: 32 4053
Tth111I GACn'n_nGTC
Cuts at: 1882 1882
Size: 4085
XbaI T'CTAG_A
Cuts at: 1116 1116
Size: 4085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 873 1580 114
Size: 759 707 2619
Fragments arranged by size:
2619 759 707
XmaI C'CCGG_G
Cuts at: 1105 1105
Size: 4085
ZraI GAC'GTC
Cuts at: 804 804
Size: 4085
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PmlI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
BaeI BfrBI BglI BmgBI BmtI BpmI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PshAI
PsiI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI